Objective:To establish a RP HPLC method to determine the content of icariin in COMPOUND XIANLINGPI INJECTION.Methods:The samples was determined by RP HPLC on an ODS column. UV detection wavelength was at 270 nm. Icariin as an external standard and CH 3OH H 2O(30∶70) as a mobile phase.Results: The average recovery was 98.32%. RSD was 1.48%.Conclusion:The method is simple and accurate. It can be used to control the quality of COMPOUND XIANLINGPI INJECTION.