Objective To research the optimal preparation technology of salinomycin micelle.Methods DSPE-PEG2000 was selected as the carrier.Salinomycin was selected as the model drug.The film dispersion method, the ethanol injection method and the dialysis method were used to prepare salinomycin micelles respectively.The comprehensive evaluation indexes included entrapment rate and drug-loading rate, release capacity and vitro cytotoxicity test in order to select the most suitable preparation technology of salinomycin micelle .Results The film dispersion method is the most suitable preparation technology of salinomycin micelle in the three methods.Its average grain diameter was (14 ±2.3) nm, entrapment rate was (82 ± 2.6)%, drug-loading rate was (6.3 ±2.1)%, IC50 to HepG2 tumor cells was 16.10 ±3.71.Conclusion The film dispersion method of salinomycin micelles has the advantages with the smallest size, the highest entrapment rate and the largest drug-loading rate, which has the function to kill tunmor cells and release slowly.

Objective To investigate the effects of autologous blood transfusion and acute normovolemic hemodilution inflamma‐tory response in patients with spinal surgery .Methods 60 patients with spinal surgery ,were randomly divided into the control group ,autologous blood transfusion group ,acute normovolemic hemodilution group ,20 cases in each group .The control group in in‐traoperative bleeding time and blood input variants of inventory .Autologous blood transfusion group was used in combination with intraoperative autologous blood recovery unit ,lose banked blood hemoglobin was low .Acute normovolemic hemodilution group via peripheral vein input must first crystal liquid or gel liquid ,then through internal jugular vein slowly pulled out the body′s blood into the special bags containing anticoagulant ,swing machine through blood kept shaking ,input when appropriate .Preoperative(T1) , After surgery 2 h(T2) ,6 h(T3) ,12 h(T4) and 24 h(T5) blood at each time point 5 mL ,detect the WBC ,IL‐6 ,TNF‐α.Records with and without postoperative complications .Results WBC in autologous blood transfusion group and acute normovolemic he‐modilution group were higher than that of preoperative ,difference was statistically significant(P<0 .05);In T2 -T5 ,serum IL‐6 , TNF‐a and the WBC concentration compared with the basis of their respective value(T1) increased significantly ,but were signifi‐cantly lower than control group ,the difference was statistically significant(P< 0 .05) .Postoperative follow‐up of the autologous blood transfusion group and acute normovolemic hemodilution group and no complications .Conclusion The autologous blood trans‐fusion group and acute normovolemic hemodilution group could effectively reduce the intraoperative and postoperative systemic in‐flammatory response ,which obviously save blood resources .

Objective To investigate and analyze the epidemiological characteristics of epidemic hemorrhagic fever (EHF),and explore the causes and possible routes of transmission in Yingkou City for proposing prevention and control measures.Methods A case restropective investigation was done to all outbreak cases of EHF in Yingkou City,relevant epidemiological investigation,clinical examination and laboratory test were done.Search of case and new case were carried out in the population.Density of rats (night clip) and rate of rat infection (Hanta virus antigenemia) in the region of the epidemic area of EHF were investigated.Blood samples were collected,antibody of EHF was detected with enzyme linked immunosorbent assay (ELISA).The rat lung was detected virus antigen using immunofluorescence.EHF diagnosis was based on Diagnostic Criteria for Epidemic Hemorrhagic Fever (WS 278-2008).Results The results of the case study showed that a family of three was infected with the same disease in one month.It was a family cluster of EHF.Of the three cases,one case was Seoul virus (SEO) positive and showed infection for rattus type.Totally 54 human serum samples from the case search in key groups were tested.Two cases were seropositive with IgG,through epidemiological investigation,all people were healthy.On physical examination they had no symptoms such as viral infection with fever,fatigue,symptoms of three red and pains of EHF.Two cases were determined latent infection.The patient's living and health conditions were very poor.The floor of indoor was soil,all of the things were placed in chaos in this family,there were many signs of rats and rat holes in the yard,and there were several rat holes around the wellhead.Rat density surveillance showed that cloth clip 108 times,effective clamp 105 times,4 rats were captured,and the density of rats was 3.81％.The density of rats exceeded the national standard of no more than 1％ of the residential area.All the rats were Rattus norvegicus.Detection of lung antigen was negative in rats by immunofluorescence.Conclusions This event is a family cluster of EHF caused by Rattus norvegicus.Rodent prevention and control,improving family conditions and environmental management are essential measures for blocking of the disease transmission and infection controlling.

【Objective】 To evaluate the performance of electrochemiluminescence immunoassay (ECLIA) in detecting HIV antigen/antibody in blood screening. 【Methods】 A total of 128 donors, reactive to anti-HIV(ELISA)testing, from September 2016 to September 2020 were enrolled, and seven samples were reactive to double anti-HIV reagents, among which 6 were confirmed by WB, 1 confirmed by NAT as negative.Two group of donors, reactive to solo anti-HIV reagent but being confirmed negative by WB(n=121) vs.randomly selected donors non-reactive to ELISA + NAT(June to September 2020, n=1360), were subjected to HIV antigen/antibody testing using ECLIA to compare the testing results, including concordance rate, sensitivity and specificity. 【Results】 The ECLIA results remained non-reactive for 1360 samples initially non-reactive to both ELISA and NAT.The concordance rate of anti-HIV reactivity by ECLIA and ELISA+ WB were 100%(6/6). For 122 samples, reactive to ELISA anti-HIV testing but nonreactive to confirmatory testing, 4(3.28%)of them were reactive to HIV antigen/antibody testing and 118(96.72%) nonreactive, with the concordance rate of ECLIA and ELISA at 96.88%(124/128). The sensitivity, specificity and false positive rate of ECLIA and ELISA were 100% vs 100%, 99.73% vs 91.77%, and 0.27% vs 8.23%, respectively. 【Conclusion】 ECLIA for HIV antigen/antibody detection has good sensitivity and specificity, which can meet the requirements of blood screening, and the false positive rate is lower than that of ELISA, adopted commonly in blood bank at present.

95%) and perfect stability,which could be quickly cleared from blood and excreted though liver and kidney.

0.05).(2)The plasma GMP-140 levels of both groups was remarkably increased immediately and a decreased trend 24 h after PCI,but did not return to normal(P

Objective To investigate the efficacy of lateral lung volume reduction with small incision and ligation for the treatment of serious chronic obstructive pulmonary disease(COPD).Methods From April 1994 to May 2006,36 patients suffering from serious COPD complicated with severe emphysema underwent lateral lung volume reduction in our hospital.Small incision and ligation were used during the operation.Results After the surgery,6 patients developed pulmonary air-leak,and were cured after 180-minute to 5-day treatments(mean,1.6 days).No perioperative death in this series.One year after the operation,the patient had significantly higher FEV1 and PaO2,and lower TLC and RV than those determined preoperation [(839.9?76.0)ml vs(589.0 ?80.5)ml,t=18.451;(72?10)mm Hg vs(62?8)mm Hg,t=4.686;(5167.6?639.9)ml vs(6725.8?976.6)ml,t=8.116;(3062.4?722.0)ml vs(4329.6?669.9)ml,t=7.720;all P=0.000].In addition,they had significantly longer 6MWT than that before the operation [(306.0?24.8)m vs(223.8?36.7)m,t=11.135,P=0.000].Conclusion For the patients with COPD who had indications for lateral lung volume deduction,resection of emphysemic tissues by the procedure using small incision and ligation can improve their symptoms and physiological conditions.

Objectives:To explore a new way in treatment of severe acute pancreatitis(SAP). Methods:From 1995 to 2001,23 patients with SAP proved by clinic and CT were treated, and compared the local artery infusion with intravenous drip on effect,mortality and time of hospitalization. Results:The mortality and time of hospitalization in 12 artery infusion and 11 intravenous drip were (14.4?3.1),(29.3?6.1) days of hospitalization and 8.33%,27.27%(mortality),respectively. Conclusions:The mortality and the time of hospitalization can be reduced by local artery infusion of medicine.

Objective: To analyze the prevalence of silicosis in the militias with contribution to the former Xiangyu Railway construction in Nanchong, China, from 2008 to 2017, and to provide a scientific basis for the future development of pneumoconiosis prevention and control strategies based on the current status. Methods: A database was established for the data of 11863 militias with contribution to the former Xiangyu Railway construction in Nanchong, China, who attended our hospital from 2008 to 2017, including 11485 males (96.81%) and 378 females (3.19%). The SPSS 17.0 software was used to analyze the differences in the detection rate of silicosis between militias with different sexes or those with different job types, as well as the age, length of service, and regional distribution of patients. Results: Of the 11863 railway militias in Nanchong, 3169 (26.71%) were diagnosed with silicosis, including 2761 (87.12%) in stage I, 359 (11.33%) in stage II, and 49 (1.55%) in stage III; the males had a significantly higher detection rate of silicosis than the females (χ²=64.496, P<0.05); there was a significant difference in the detection rate of silicosis between the militias with different job types (χ²=856.839, P<0.05). There were significant differences in the mean age of diagnosis and the mean length of service between the patients with different stages of silicosis (F=4.944, P<0.05; F=3.174, P<0.05). There was a significant difference in the number of militia patients with silicosis between different areas of Nanchong (χ²=843.330, P<0.05). Conclusion The prevalence of silicosis is relatively high among the militias with contribution to the former Xiangyu Railway. It is necessary to strengthen the occupational health monitoring of patients, actively treat and prevent complications, and improve the quality of life of patients.

BACKGROUND: Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is a kind of cytokine which can stimulate the differentiation and proliferation of haematopoictic precursor cells and plays an important role in the process of immunoloregulation. Escherichia coli (E.coli) expression system has advantages,such as low cost and high output, over other systems.Therefore, E.coli is still the most commonly used exogenous gene expression system.OBJECTIVE: To observe the expression of hGM-CSF in the E.coli swain DH5α.DESIGN, TIME AND SETTING: The present observational experiment was performed at the Central Laboratory of Biotechnology Research,China Three Gorges University between February and June 2007. MATERIALS: Plasmid pBR322hGM-CSF was purchased from American Type Culture Collection, USA.hGM-CSF antibody was sourced from Sigma Company, USA.IPTG, X-gal,and vector pMGT-18 were purchased from Shanghai Bioengineering Technology Company, China. METHODS: Primer was designed according to hGM-CSF gene. Taking cloning vector plasmid pBR322-hGM-CSF as template, hGM-CSF gene was acquired and recombined into prokaryotic expression vector pGEX-4T-1. Recombinant plasmid confirmed by enzyme digestion and sequencing was transformed into E.coli strain DH5α and induced by isopropy-β-D-thiogalactoside (IPTG). Expression product was identified by sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE) and detected by Western blotting assay. MAIN OUTCOME MEASURES: hGM-CSF expression in the E.coli strain DH5α. RESULTS: SDS-PAGE results revealed that the recombinant hGM-CSF protein with relative molecular weight of 40 500 was produced up to approximately 17.9% of total protein. Western blotting detection results indicated that there was a 40 500 bp brand. CONCLUSION: The present study reconstructed prokaryotic expression vector pGEX-hGM-CSF, induced its expression in the E.coli strain DH5α, and obtained hGM-CSF fusion protein.