0.05)and could be induced significantly after 12 h (P

ObjectiveTo study the expression and clinical significance of osteopontin in nasopharyngeal carcinoma tissues.MethodsOsteopontin expression was determined in nasopharyngeal carcinoma tissues in 44 cases and normal mucosa tissues in 25 cases by immunohistochemical staining.The relationship between osteopontin expression and pathological features of nasopharyngeal car(e)inoma was analyzed.ResultsThe positive expression rate of osteopontin in nasopharyngeal carcinoma tissues was significantly higher than that in normal tissues and the positive staining area was larger [77.27％(34/44) vs.8.00％(2/25),(60.24 ± 17.51 )％ vs.(1.32 ± 0.48)％ ](P＜0.05).The positive expression of osteopontin was correlated with clinical staging and lymph node metastasis(P ＜ 0.05),while had no relationship with pathological staging(P＞ 0.05).ConclusionsThere is a close correlation between expression of osteopontin and tumor cell invasion and metastasis.Over-expression of osteopontin may be one of the important factors contributing to the invasion and migration of nasopharyngeal carcinoma.

Objective:To observe whether the Simiao Yongan Decoction regulate the nuclear factor-?B(NF-?B) expression and related factors in atherosclerosis(AS)rabbit model,thus restrain the occurrence of atherosclerosis and plaque formation.Methods:56 male Japanese rabbits were randomly divided into normal group,model group,Simvastatin group and Simiao Yongan Decoction group,in addition to the normal group,other groups were established aortic atherosclerotic plaque model.From the1st day of experiment,the corresponding drug intervention began,10 weeks later,the rabbits were killed over the weekend after the anesthesia line access aortic NF-?B immunohistochemical staining,and the experimental dynamic 0,3,6,10 weeks in serum TNF-?,IL-1 and MCP-1 content.Results:The expression of NF-?B p65 subunit in model group was rich,and compared with it,the expression of NF-?B p65 subunit in treatment groups decreased,and the Simiao Yongan Decoction can obviously inhibit the expression of NF-?B p65 subunit.In model group,the expressions of IL-1,TNF-?and MCP-1 in serum increased significantly at different time points(P

This study was aimed to determine effect of Bu-Shen Kang-Shuai (BSKS) Tablet on HO-1 mRNA and its associated oxidative stress levels among atherosclerotic rabbits. A total of 56 rabbits were randomly divided into the normal group (8 rabbits) and the experimental group (48 rabbits). Normal diet was given to the normal group. Atherosclerotic rabbits models were established in the experimental group. At the eighth week, rabblits in the experi-mental group were randomly divided into the model group, BSKS Tablet group and simvastatin group. Blood samples were collected before medication, 8-, 12-, 16-week after medication from rabbits of each group. Rabbits were sacri-ficed under aseptic conditions at the last blood collection. Expressions of aortic HO-1 mRNA and PPARα mRNA were measured by Q-PCR method. The level of MMP-9 was measured by immunohistochemical assay. Serum HbCO, COX-2 activity and cGMP level were measured by ELISA assay. The results showed that after the intervention of BSKS Tablet, serum HbCO level decreasd, cGMP was obviously increased. However, there was no obvious change on the COX-2 activity. The immunohistochemical assay showed that BSKS Tablet obviously reduced MMP-9 level of rabbits. There was only small amount of aortic HO-1 mRNA expression in the normal group. However, the expres-sion of aortic HO-1 mRNA in the atherosclerosis group was increased. After intervention of BSKS Tablet, the ex-pression of HO-1 mRNA was increased with statistical significance (P < 0.05). Simvastatin had similar antioxidant effect. It was concluded that the compound preparation of traditional Chinese medicine (TCM) BSKS Tablets had an important antioxidant effect in treatment of atherosclerosis. Its protective mechanism may be through the regulation of HO-1 mRNA gene expression and effects of HO-1/CO-cGMP pathway activities of related enzymes while peroxida-tion stability of atherosclerotic plaque.

Objective To observe the syndrome manifestations of rabbit atherosclerosis models and explore the syndrome attribution rules of the models.Methods The 24 male Japanese rabbits were randomized into a control group(n=8)and a experimental group(n=16).The control group was given normal feed,and the experimental group was treated with high-fat diet and immune injury and surgical injury through the femoral artery balloon.They were fed totally for 10 weeks.Vascular morphological changes and blood lipid determine were used to evaluate the models.The ear,tongue,eye,and the overall state of rabbits were regularly observed to see the changes of TCM syndromes.Results The rabbits of the experimental group gradually reduced the amount of activity in early stage.When the climate changed,their nasal secretion was increased,and they had wheezing breathing,fever and other common cold symptoms like red auricle.In the medium stage,the fatty plaque or belt around the eyes,cold auricle,and listlessness could be seen,and the blood fat was significantly higher than that of the control group.To the tenth week,the dark tongue with blood spots and purple cold auricle appeared in the rabbits of the experimental group.It was difficult to draw blood from the ear,and the emboli could be seen in the vessels of auricles.Compared with the control group,the oxidized low density lipoprotein and malondialdehyde of the experimental group were significantly increased,and the activity of superoxide dismutase decreased.Conclusion The syndromes of rabbit atherosclerosis models are in the changing state,in early stage they can be manifested as Qi deficiency,in middle stage the manifestation can be Qi deficiency with phlegm retention,and in late stage there will be Qi deficiency with blood stasis.The rule is similar to that of the pathogenesis of atherosclerosis in clinic.