The changes of CaM content and distribution in acute cerebral ischemia of Mongolian gerbils were investigated with enzyme-linked immuno-sorbent assay (ELISA )and immunogold staining(IGS). The ELISA results showed that which ligation of both common carotid arteries for 10 rain the contents of CaM were significantly lower (P

The effects of bepridil, ni-modipine and nicardipine on the activity of CaM - dependent protein kinase Ⅱ from mouse cerebrum were studied in vitro. Tt was found that bepridil significantly inhibitied the activity of CaM - PK Ⅱ with an IC50 value of 0. 3 mmol ? L-1. but nimodipine and nicardipine did not basi-cally influence the activity of the enzyme. The experimental results showed that the inhibitory effect of bepridil on the activity of CaM - PK Ⅱ was an antagonistic action of bepridil to CaM.

0.1), while in Yunnan Baiyao group, there were significant reductions in all scores (P

Objective To compare the preemptive analgesia effect of flurbiprofen axetil with tramadol in patients undergoing laparoscopic hysterectomy. Methods Ninety patients undergoing laparoscopic hysterectomy were divided randomly into group A (n=30), group B (n=30) and group C (n=30). The patients in group A were administrated 1mg/kg flurbiprofen axetil intravenously before tracheal intubation, and in group B 2mg/kg tramadol intravenously before tracheal intubation, while in group C 1mg/kg flurbiprofen axetil were administrated intravenously at the end of operation. The postoperative analgesic effects (1, 2, 4, 8, 12, 24 hours) were evaluated by VAS score and BCS score, while the times of pain-killer boosting and the side effects were recorded. Results VAS scores of group C were significantly higher than those of group A and B at all time points (P<0.05), except for 12 hours and 24 hours after the surgery, while BCS scores of group C Were lower than those of group A and B at the same time points (P<0.05).The side effects of group A and group C were significantly lower than those of group B (P<0.05). Conclusion Preemptive analgesia with flurbiprofen axetil and tramadol in patients undergoing laparoscopic hysterectomy is effective and convenient, while flurbiprofen axetil has less side effects.

The feature of TCM is embodied in its unique thought style. Standing at TCM self thought rule, it discusses the future development of TCM, pointing out that TCM development must preserve traditional thought feature, thereby reform present TCM thought method and set up modern TCM thought system.

Object To compare the contents of chlorogenic acid in the different processed crude drugs of Lonicera fulvotomentosa Hsu et S.C. Cheng. Methods The contents of chlorogenic acid were deter mined by HPLC. Chromatographic conditions included Hypersil C 18 column and the mobile phase consisting of a mixture of acetonitrile 0 4% phosphoric acid (13∶87). Chlorogenic acid was detected at 327 nm wavelength. Results The contents of chlorogenic acid were 0 54%, 0 81%, 0 57% in the sun cured flower bud, white bloom, gold flower in L. fulvotomentosa and 2 14%, 1 65%, 2 04% in the steaming sun cured crude drugs, respectively. Conclusion The contents of chlorogenic acid in the steaming sun cured crude drugs are higher than those in the sun cured crude drugs.

Objective To analyze the significant impact factors of postoperative hemorrhage after pancreaticoduodenectomy.Method The clinical data of 154 patients who underwent pancreatieoduodeneetomy from Jan.2009 to Jun.2012 were retrospectively analyzed.Results The incidence of postoperative hemorrhage was 10.4％ (16/154).The mortality caused by this complication was 12.5％ (2/16).Among these patients,intra-abdominal hemorrhage occurred in seven patients,and gastrointestinal hemorrhage occurred in nine patients.One patient suffered from both these complications.There were five and two patients presenting with early or delayed intra-abdominal hemorrhage,respectively.Early and delayed gastrointestinal hemorrhage occurred in three and six patients,respec tively.Statistical analysis showed that infection (P=0.002,P＜ 0.01) and pancreatic fistula (P=0.048,P＜0.05) are significantly associated with postoperative hemorrhage.Conclusions Postoperative hemorrhage after pancreaticoduodenectomy is a severe complication.Adequate hemostasis and prevention of pancreatic fistula and infection are the key points in reducing postoperative hemorrhage.Proper treatments should be used according to the site,type of onset and severity of hemorrhage.

Objective To investigate the relationships between Cyclooxygenase(COX-2) and angiogenesis and expressions of COX-2 and vascular endothelial growth factor(VEGF) in thyroid cancer and its clinical significance.Methods Immunohistochemistry was used to detect the expressions of COX-2 and VEGF in 60 thyroid cancer,(15 thyroid)adenomas and 10 normal thyroid tissues.Results The expression rates of COX-2 and VEGF in thyroid cancer were higher than those in thyroid adenomas and normal tissues(P

Objective To understand the status of human resources for AIDS control at city and county level in Hebei Province,and to improve the outfit of human resources.Methods General investigation and self-designed questionnaire were used by the trained investigators.Epi Data 3.0 was used to establish a database,and SPSS 13.0 was used to analyze the data.Results There were 1 347 missionaries at the city and county level,547 of whom were full-time staff.Significant differences existed in academic qualifications and titles between city and county level.A lack in technical staff in county level appeared.Conclusions The number of staff for HIV control in Hebei is not sufficient.Therefore human resources in these places should be strengthened and rationally allocated for AIDS control.

Objective To explore the role of tetramethylpyrazine (TMP) in protection of homocysteine (HCY)-induced apoptosis of hippocampal neurons in mice. Methods One hundred healthy adult Kunming mice of clean grade were randomly divided into 5 groups, 20 for each group. HCY was injected into lateral ventricle using stereotaxic technique. FJB staining was used to detect neural apoptosis and the Morris water maze test was applied to assess memory and cognitive abilities. Results FJB staining showed that apoptosis of hippocampal neurons in the mice treated with TMP at different doses decreased significantly as compared with that in HCY group, especially at a dose of 20 g/L. Conclusions TMP can alleviate HCY-induced damage of the hippocampal neurons in mice and improve memory and cognitive functions, which suggests TMP has a potential clinical value in prevention and treatment of Alzheimer′s disease.