BACKGROUND: Studies have confirmed that nerve growth factor can promote wound tissue to release al kinds of endogenous growth factors and growth factor receptors, which play a positive regulatory role. The nerve growth factor can promote cel proliferation and accelerate wound healing, thus making the wound healing developed from the passive waiting healing to active control healing. OBJECTIVE: To investigate the effects of local application of nerve growth factor on the expressions of transforming growth factor β1 and basic fibroblast growth factor in rat burn wounds. METHODS: Twenty-four adult Sprague Dawley rats were used in the study, and Ⅱ degree deep burn wound was made on the back of rats. Then, these rats were randomly divided into four groups. After burn wound debridement, the wounds were covered with gauzes containing 1, 2.5 and 5 μg/mL nerve growth factor solution and normal saline respectively. At 3, 5, 9 and 14 days after treatment, the wound healing time and percentage of residual wound were observed. Then, wound tissues were cut for histological examination, in order to detect the expressions of transforming growth factor β1 and basic fibroblast growth factor in wounds, as wel as the cel ular DNA cycle. RESULTS AND CONCLUSION: The wound healing time in the treatment groups was shorter than that in the control group, especial y in 5 ug/mL nerve growth factor treatment group (P < 0.01), and the percentage of residual wound in the treatment groups was less than that in the control group. The histological examination showed the number of nucleated cells in the superficial dermis of the treatment groups was significant increased when compared with that in the control group; the expressions of transforming growth factor β1 and basic fibroblast growth factor in the treatment groups at different time points were stronger than those in the control group, and the expressions at 5 and 9 days were stronger than those at 3 and 14 days; percentage of cells in S phase of the treatment groups was significantly increased, especial y in 5 mg/L nerve growth factor group (P < 0.01). The results indicate that local application of nerve growth factor can accelerate wound healing by increasing the expressions of transforming growth factor β1 and basic fibroblast growth factor, stimulating mitosis and promoting proliferation.

OBJECTIVETo study the effects of Chrysanthemum morifolium based on functional diversity in soil microbial community with different cropping system and fertilization and offer scientific basis for the establishment of the reasonable planting patterns.

METHODCombined yield and quality of Ch. morifolium, 8 treatments of different cropping system and fertilization on functional diversity in soil microbial community of Ch. morifolium were investigated by the Biolog.

RESULTThe AWCD of Ch. morifolium paddy-dryland rotation was higher than that of the continuous cropping, the AWCD of organic fertilizer and compound NPK > single organic fertilizer > single compound NPK > no fertilizer. The principal component analysis about Ch. morifolium soil microbial carbon source use showed that the contribution rate of principal component 1 was 45.5% and principal component 2 was 12.1%, which could explain most information about the variation. Different cropping system of Ch. morfolium differentiated in principal component 1 axis, different fertilization differentiated in principal component 2 axis. The yield of Ch. morifolium and volatile oil content paddy-dryland rotation was significantly higher than that of continuous cropping.

CONCLUSIONThe Ch. morifolium should be cultivated with organic fertilizer and compound NPK by paddy-dryland rotation patters.

Biota ; Chrysanthemum ; metabolism ; microbiology ; Fertilizers ; Oils, Volatile ; analysis ; Soil Microbiology

Objective:To investigate whether the combined injection of micronized acellular dermal matrix(mADM) and granular fat can improve the volume retention rate of the graft and the optimal mixture ratio of these two elements.Methods:From November 2022 to March 2023, the inguinal fat of rats was collected from the Animal Experiment Department of the 940th Hospital of the Joint Logistics Support Force of PLA, and then mixed with mADM in different proportions. Subsequently, different mixtures were injected into both sides of the back in another group of 15 rats: granular fat group (control group, C1), mADM group(control group, C2), granular fat+ mADM(1∶1)group(experimental group, E1), granular fat+ mADM(2∶1) group (experimental group, E2), granular fat+ mADM(4∶1) group(experimental group, E3), granular fat + mADM(6∶1) group (experimental group, E4), and 0.5 ml was injected in each group. At the 4th week, 8th week and 12th week, 5 specimens were randomly selected to detect the volume of grafts, histological analysis and neovascularization of grafts. SPSS 25.0 software was used for statistical analysis. Repeated measures analysis of variance was used to compare the data at multiple time points among multiple groups, and one-way analysis of variance was used to compare the data at each time point among multiple groups. P<0.05 was considered statistically significant. Results:The volume of the grafts in the 6 groups changed significantly with time, and the difference was statistically significant ( P <0.05). At the 4th, 8th and 12th week after injection, the volume of C2 group was larger than that of C1 group. The histological analysis showed that there was no obvious inflammatory cell infiltration in the early stage of C2 group, and the inflammatory reaction gradually occurred after 8 weeks. In the C1 group, vacuolization was observed, and the inflammatory cell infiltration was obvious. The larger the proportion of mADM and granular fat mixed injection, the more collagen fibers arranged regularly. There was a significant difference in the number of neovascularization among the 6 groups at the 4th week after transplantation ( P<0.05), but there was no significant difference at the 8th and 12th week ( P>0.05). Conclusion:mADM combined with granular fat injection can effectively maintain the volume of injection for a long time, and more suitable effect is achieved when the two are mixed at a ratio of 1∶1.

Objective:To investigate whether the combined injection of micronized acellular dermal matrix(mADM) and granular fat can improve the volume retention rate of the graft and the optimal mixture ratio of these two elements.Methods:From November 2022 to March 2023, the inguinal fat of rats was collected from the Animal Experiment Department of the 940th Hospital of the Joint Logistics Support Force of PLA, and then mixed with mADM in different proportions. Subsequently, different mixtures were injected into both sides of the back in another group of 15 rats: granular fat group (control group, C1), mADM group(control group, C2), granular fat+ mADM(1∶1)group(experimental group, E1), granular fat+ mADM(2∶1) group (experimental group, E2), granular fat+ mADM(4∶1) group(experimental group, E3), granular fat + mADM(6∶1) group (experimental group, E4), and 0.5 ml was injected in each group. At the 4th week, 8th week and 12th week, 5 specimens were randomly selected to detect the volume of grafts, histological analysis and neovascularization of grafts. SPSS 25.0 software was used for statistical analysis. Repeated measures analysis of variance was used to compare the data at multiple time points among multiple groups, and one-way analysis of variance was used to compare the data at each time point among multiple groups. P<0.05 was considered statistically significant. Results:The volume of the grafts in the 6 groups changed significantly with time, and the difference was statistically significant ( P <0.05). At the 4th, 8th and 12th week after injection, the volume of C2 group was larger than that of C1 group. The histological analysis showed that there was no obvious inflammatory cell infiltration in the early stage of C2 group, and the inflammatory reaction gradually occurred after 8 weeks. In the C1 group, vacuolization was observed, and the inflammatory cell infiltration was obvious. The larger the proportion of mADM and granular fat mixed injection, the more collagen fibers arranged regularly. There was a significant difference in the number of neovascularization among the 6 groups at the 4th week after transplantation ( P<0.05), but there was no significant difference at the 8th and 12th week ( P>0.05). Conclusion:mADM combined with granular fat injection can effectively maintain the volume of injection for a long time, and more suitable effect is achieved when the two are mixed at a ratio of 1∶1.

Objective:To investigate the feasibility and clinical effects of using free perforator propeller myocutaneous flap from buttock in repairing complex wounds in the buttock with deep dead cavity.Methods:A retrospective observational study was conducted. From June 2020 to June 2022, 9 patients with complex wounds in the buttock with deep dead cavity who met the inclusion criteria were admitted to Lanzhou University Second Hospital, including 6 males and 3 females, aged 26 to 62 years, with original wound area ranging from 4.0 cm×3.0 cm to 8.0 cm×7.0 cm and dead cavity depth of 7 to 11 cm. All the wounds were repaired with free perforator propeller myocutaneous flap from buttock, with flap area of 6.0 cm×2.5 cm to 13.0 cm×7.0 cm and muscle flap length of 6 to 11 cm. All the wounds in the donor area were closed and sutured directly. Postoperative myocutaneous flap survival, complications, as well as donor and recipient wound healing were observed, and the shape of donor and recipient areas were followed up.Results:Congestion occurred under the myocutaneous flap of one patient due to poor drainage on post surgery day 2, which was healed after 15 days of drainage and dressing change. The myocutaneous flaps of other patients survived successfully after surgery. The wounds in the donor and recipient areas were all well healed. During the follow-up of 3 to 10 months, the donor and recipient areas were full in shape, with little difference from the healthy side, and were able to bear pressure.Conclusions:The free perforator propeller myocutaneous flap from buttock can repair the deep dead cavity and surface wounds at the same time. The use of this myocutaneous flap in repairing complex wounds in the buttock with deep dead cavity results in minimal damage to the donor area, allows pressure-bearing of the donor and recipient areas after surgery, and ensures a full buttock shape.