AIM: To study fibroblast proliferation and collagen synthesis during wound healing in diabetic rats induced by streptozotocin. METHODS: 30 Wistar male rats were randomly divided into control group and model group. 55 mg/kg STZ were given intraperitoneally to model rats. After 3 weeks, a round skin of 2.04 cm~2 was excised on all dorsal back of rats. The healing time and healing rate were observed according to re-epithelization. The numbers of fibroblasts and the expression of proliferating cell nuclear antigen (PCNA) were observed by Hematoxylin-Eosin (HE) staining and immuno-histochemistry assay. Collagen Ⅰ and Ⅲ stained by Picric acid-Sirius red were calculated by image analysis. RESULTS: The healing time in model group was (27.13?1.81) days,significantly longer than that in control group [(15.25?1.67) days, P

Objective To observe the clinical effects of modified Shengxian decoction in the treatment of thyroid cancer postoperative hypothyroidism patients.Methods 60 patients of thyroid cancer postoperative hypothyroidism due to deficiency of spleen and kidney were randomly divided into a control group and a treatment group.The control group (28 cases) was by levothyroxine sodium tablets conventional treatment,while the treatment group (32 cases) was treated with Shengxian decoction based on thecontrol group.The changes of the symptom integral and HAMD-17 were observed between two groups before and after treatment.Results After treatment,the total effective rate was 87.5％ in the treatment group and 64.3％ in the control group,the clinical effect of treatment group was significantly better than the control group (x2=4.499,P＜ 0.05).After treatment,the total score of HAMD-17 was 6.8±2.3 in the treatment group and 15.8±2.1 in the control group,both significantly improved than that before the treatment (which were 19.4±1.45 and 18.9± 1.32 respectively).Compared with the control group,the treatment group improved significantly (P＜ 0.01).Conclusion Shengxian decoction plus western medicine has remarkable curative effect in the treatment of thyroid cancer postoperative hypothyroidism,it can improve the depressive state of patients and improve the quality of life.

AIM: To study the effects of Yangyu Tuji (YYTJ) on delayed healing wound of diabetic rats caused by streptozotocin (STZ). METHODS: SD male rats were randomly divided into control group (control), model group (model); and 3 different dose groups of YYTJ. 55 mg/kg STZ were given by intraperitoneal injection except for control group. After 30 days, a round skin of 1.6 cm diametre was excised on all dorsal back of rats. The healing time and healing rate were observed according to re-epithelization. The content of collagenⅠ and Ⅲ was observed by Picric acid-Sirius red staining , Matrix metalloproteinase-1, 13 (MMP-1, -13), tissue inhibitor of metalloproteinases-1 (TIMP-1) by immuno-histochemistry assay. All data were analyzed by IPP software. RESULTS: The healing time in each group treated with YYTJ was shorter than that in model group (P

The 81st annual scientific meeting of the American Diabetes Association (ADA) was held online from June 25 to 29, 2021. The conference encompassed following theme areas: Acute and chronic complications of diabetes, behavioral medicine, clinical nutrition, education and exercise, clinical diabetes/therapeutics, epidemiology/genetics, immunology/transplantation, insulin action/molecular metabolism, integrated physiology/obesity and islet biology/insulin secretions, which covers both clinical and basic aspects of diabetes mellitus and other metabolic diseases. Here is the summary of the main points of academic progress in diabetes and other metabolic diseases.

Objective To study the effect of Xiaoyin Jiedu Fang, a Chinese medicine compound formulated by TCM principle of cooling blood with detoxification, on nonreceptor tyrosine kinase JAK1/ signal transducer and activator of transcription 3 (STAT3) signaling pathway in human acute leukemia Jurkat T cells.Methods The abnormal activation of T cells of psoriasis with TCM pattern of blood-heat was induced by stimulating Jurkat T cells with phytohaemagglutinin (PHA) and IL-6.The medicated serum contained Xiaoyin Jiedu Fang granule (XYJDF), Liangxue Fang (LXF) or Jiedu Fang granule (JDF) which was a disassembled prescription of XYJDF, acitretin (positive drug) and distilled water (negative control) were prepared.The model cells were inoculated into 24-hole cell culture plate and were cultured with the prepared medicated serum and Filgotinb(GLPG0634), an inhibitor of JAK/STAT signaling pathway for 48 hours.Jurkat T cells with no treatment was served as the blank group.Protein and mRNA expression of JAK1, STAT3, glucoprotein(GP)130 were detected by using Western blot and real-time PCR.Results The protein and mRNA expression of JAK1,STAT3 and GP130 in the model group were significantly higher than those of the blank group (P<0.01).Compared with the model group, the protein expression of pJAK1/JAK1, pSTAT3/STAT3 and GP130 in all medicated group were decreased significantly (P<0.01), and the mRNA expression of JAK1, STAT3 and GP130 declined (P<0.01 or P<0.05).Conclusion The effect of XYJDF against psoriasis could be via the way of inhibiting JAK1/STAT3 signaling pathway leading to prevent abnormal activation of T cells.

Background::Given the established genetic linkage between triggering receptors expressed on myeloid cells 2 (TREM2) and Alzheimer’s disease (AD), an expanding research body has delved into the intricate role of TREM2 within the AD context. However, a conflicting landscape of outcomes has emerged from both in vivo and in vitro investigations. This study aimed to elucidate the multifaceted nuances and gain a clearer comprehension of the role of TREM2. Methods::PubMed database was searched spanning from its inception to January 2022. The search criteria took the form of ( "Alzheimer’s disease" OR "AD" ) AND ( "transgenic mice model" OR "transgenic mouse model" ) AND ( "Triggering receptor expressed on myeloid cells" OR "TREM2" ). Inclusion criteria consisted of the following: (1) publication of original studies in English; (2) utilization of transgenic mouse models for AD research; and (3) reports addressing the subject of TREM2.Results::A total of 43 eligible articles were identified. Our analysis addresses four pivotal queries concerning the interrelation of TREM2 with microglial function, Aβ accumulation, tau pathology, and inflammatory processes. However, the diverse inquiries posed yielded inconsistent responses. Nevertheless, the inconsistent roles of TREM2 within these AD mouse models potentially hinge upon factors such as age, sex, brain region, model type, and detection methodologies.Conclusions::This review substantiates the evolving understanding of TREM2’s disease progression-dependent impacts. Furthermore, it reviews the interplay between TREM2 and its effects across diverse tissues and temporal stages.