Objective: To investigate the effects of silk fibroin on the immobilization of thrombin. Methods: The immobilized thrombin was prepared using silk fibroin as the carrier and glutaraldehyde as the crosslinking agent. With activity yield as the index, the process conditions of silk fibroin immobilized thrombin were determined by an orthogonal test. Results:The optimum process con-ditions of immobilized thrombin treated with silk fibroin were as follows:the immobilization time was 6 h, the enzyme dosage was 2 400 NIH·g-1 casein, the temperature was 25℃ and pH was 7. 6. The activity recovery of immobilized thrombin was 67. 22%. Conclu-sion:Silk fibroin has the positive immobilization effect on thrombin.

AIM: To compare anterior segment parameters in eyes with cataracts and different axial lengths measured with Pentacam and IOL Master.

METHODS: This study included 170 eyes of 143 patients diagnosed as cataract at Tangdu Hospital, Air Force Medical University. For each patient, anterior segment parameters were measured before surgery with Pentacam and IOL Master system, respectively.

RESULTS: There were no statistically significant differences in K1 and K2 values of eyes with long axial length(P>0.05). And there were statistically significant differences in K2 values(P<0.01), but not K1 values(P>0.05)of eyes with short and middle axial lengths. There were statistically significant differences in ACD values of eyes with any axial length(P<0.01). The Bland-Altman showed that ACD values of eyes with any axial length and K2 values of eyes with long and middle axial lengths were both in good agreement, and K1 values of eyes with any axial length were in great agreement.

CONCLUSION: Pentacam and IOL Master can be interchanged for keratometry in eyes with long axial length, but the measurements of keratometry in eyes with short and middle axial lengths and ACD of eyes with any axial length should be compared in clinical application.