Objective To investigate the expression of stratum corneum chymotuptic enzyme (SCCE) and its significance in human cervical adenocarcinoma.Methods Immunohistochemistry was used to detect the expressions of SCCE,ER,CEA and Vimentin in tissues from the group of 45 cervical adenocarcinoma patients,group of 24 cervical glandular intraepithelial neoplasia (CGIN) patients and group of 24 normal cervical tissues.Results The expression of SCCE was 84.44 ％ (38/45),58.33 ％ (14/24) and 8.33 ％ (2/24) in 3 groups respectively.The SCCE expression from group of cervical adenocarcinoma were more significantly than that of normal cervical tissues (P ＜ 0.012 5),the expression of SCCE from group of CGIN were more significantly than that of normal cervical tissues (P ＜ 0.012 5),but there was no difference between the cervical adenocarcinoma and the CGIN on the expression of SCCE (P ＞ 0.012 5).Conclusion SCCE could be recommended as a new biomarker for the early screening cervical adenocarcinoma and as an additional marker for the prognosis of cervical adenocarcinoma.

MicroRNAisatypeofsingle-strandednon-codingRNA,directlybondingtothecomplemen-tary target gene mRNA.That leads to the inhibition of mRNA translation of the target molecule in order to reduce expression of the target gene.MicroRNAs are not only involved in the regulation of inflammatory reac-tion,but also play important roles in the formation of neoplasm.The researches showing that the inflammatory reaction interacting with miRNAs results in the differential expressions of microRNAs in lung cancer have been widespreadly concerned.Along with the deepening of the research on the mechanisms of inflammatory reaction and differential expression of microRNAs,it will provide new strategies for the prevention,diagnosis and treat-ment of lung cancer.

Three hundred and nine community health service (CHS) practitioners were selected with randomized cluster sampling for survey with the job satisfaction questionnaire-occupational stress indicator (OSI).Degree of the job satisfaction was (68 ± 12)%.Position, work experience, income,feeling of recognition, degree of motivation, the scope for career development, the space for personal ambition and the relationship between salary and work load were potential influencing factors of job satisfaction to CHS practitioners.The authors suggest to establish the dynamic mechanisms of satisfaction study and to formulate comprehensive and effective incentive measures for CHS practitioners.

Objective To summarize our experience in the prevention and treatment of iatrogenic vascular(trauma).Methods The clinical data of 24 different types of iatrogenic vascular trauma committed from 2003 to 2006 were retrospectively analyzed.Results The 24 cases included 7 cases of superior mesenteric(arteriovenous) trauma,3 cases of portal venous trauma,4 cases of carotid arterial trauma,6 cases of iliac and femoral arterial trauma,and each one of trauma of popliteal artery,axillary artery,renal artery,left gastric artery,respectively.Treated method: Six cases underwent vascular repair,5 cases had vascular anastomosis,2 cases had vascular replacement,3 cases had vascular ligation,2 cases had covered stent implantation under intervention,and other methods included thrombectomy,thrombolysis and packing.Among the 24 cases,22 were cured completely,one patient died from massive hemorrhage 24 hours after operation,and the other died 5 days after operation.Conclusions Iatrogenic vascular trauma can be prevented and its incidence reduced by increased vigilance,clear identification of the anatomy,and accurate and careful operation.Once iatrogenic vascular trauma has occurred,its cause must be determined and then different treatment methods can be(chosen),based on the cicumstaces. When effective treatment technique is not at hand,one should promptly seek outside support or transfer the patient to an advanced hostipal.

Dongting Lake area is one of the major marshland schistosomiasis endemic areas in China. In recent years spatial epidemiology is widely used in the research of schistosomiasis which is a new opportunity to break through the current wander-ing situation of schistosomiasis control. In this article both the generalized and Dongting-Lake-specific epidemic indicators of schistosomiasis are reviewed to provide the basis to construct the schistosomiasis Geographic Information System GIS database of Hunan Province.

Aim To examine the inhibitory effect of re-combinant cardiac troponin fusion protein composed of subunit I and artificial peptide which was called CIS on tumor growth. Methods The CIS ’ s effect on the growth of human umbilical vein endothelial cells ( HU-VEC) was examined using MTT assay in vitro. Chick chorioallantoic membrane model was applied to study the alteration of angiogenesis treated with purified re-combinant CIS protein. The effect of tumor growth trea-ted with CIS was observed using several in vivo mice xenograft models. Results There was a statistically significant reduction in HUVEC cell proliferative rate when the cells were treated with purified CIS fusion protein, which was also shown in a dose-dependent manner. A decreased amount of new blood vessel for-mation ( angiogenesis) on chick embryo chorioallantoic membranes was observed in recombinant CIS protein treated group compared to the untreated control group. A significant inhibition of tumor growth rate was a-chieved in CIS treated mice compared to CIS untreated control mice in 6 different mouse xenograft models. Conclusions The fusion protein CIS shows the inhibi-tory effect on the tumor growth in our in vivo mouse models, and such inhibition could be mediated by the mechanism of CIS’ s effect on the decrease of HUVEC cell proliferation and further the reduction of angiogen-esis in tumor tissues. This work could provide the foundation for the in-depth investigations on the phar-maceutical application of CIS targeting anti-tumor ther-apy.

BACKGROUND: Permanent or transient implantation of biomaterials can result in biomaterials-centered infections (BCI) in lung cancer patients.OBJECTIVE: To investigate the relationship between BCI and peripheral blood transforming growth factor β1 (TGF-β1) in patients with lung cancer.METHODS: A total of 248 lung cancer patients undergoing in vivo intravascular catheter indwelling > 7 days were included.Quantitative method was used for intubation, bacteriological culture and paired blood culture, and API Staph strips were adopted for positive patients. While enzyme-linked immunosorbent assay was used to detect TGF-β1 levels in the peripheral blood of patients with lung cancer and 75 healthy volunteers as normal controls.RESULTS AND CONCLUSION: Among the 248 patients, there were 82 BCI-positive cases, and 166 BCI-negative cases.Thirteen patients were confirmed to have catheter-related bloodstream infection. There were 48 Gram-positive bacteria, 24Gram-negative bacilli, and 10 fungal. The levels of TGF-β1 were higher in BCI-positive patients than BCI-negative patients (P < 0.05); the levels of TGF-β1 in the BCI-negative group were higher than those in the normal control group (P < 0.05). For lung cancer patients with nosocomial infection induced BCI, there are various species of pathogenic bacteria, and Gram-positive bacteria are more common. To detect TGF-β1 levels in patients with lung cancer is of significance for early prevention of BCI.

Objective To study the effect of epinephrine on biofilm formation of the qseC-deleted mutant of Escherichia coli on biomaterial.Methods The strains used in this study are Escherichia coli MC1000 and MC1000AqseC.LB was used for all the experiments.To determine the effect of epinephrine on motility,halos were measured in LB medium at 37℃ in the presence of epinephrine(50 μmol/L).LB with epinephrine and without epinephrine were used,and then the experiment of bacterial biofilm formation on PVC material was taken.The relative amount of biofilm was estimated.The thickness of bacterial community and bacterial community quantity in the unit area on PVC materials were measured by confocal laser scanning microscope( CLSM),and the surface structure of biofilm formation was observed by scanning electron microscope(SEM).Results The mutant strain formed less biofilm than the wild-type strain in LB.The increment in motility of wild-type strain due to epinephrine addition was shown,but mutant strain is unaffected.Similarly,biofilm formation of the wild-type strain was increased by epinephrine,but epinephrine did not affect the biofilm formation of the qseC mutant.The CLSM and SEM showed that epinephrine stimulated biofilm formation of wild-type strain on PVC materials,but had no effect on qseC-deleted mutant strain.Conclusion Epinephrine increases Escherichia coli biofilms on biomaterials through qseC.

BACKGROUND:Studies have demonstrated that prosthetic valve endocarditis is primarily caused by bacteria adhering on the surface of the materials.Thus,the relationship of prosthetic valve materials with bacteria adhesion and growth is an important subject.OBJECTIVE:To explore the influence of prosthetic valve materials on bacteria growth through observing the relationship of bacteria adhesion on prosthetic valve materials and bacteria growth.DESIGN,TIME AND SETTING:Repetitive measurement was performed at the Department of Cardiac and Thoracic Surgery,Third Hospital of Kunming Medical College from January to March 2001.MATERIALS:Terylene(Dacron)was purchased from Man-made Blood Vessel Laboratory of Suzhou Weaves Belt Factory;polytetrafluoroethylene was provided by Teflon-GoreTexW.L.Gore & Associates,Inc.Arizona,USA;pyrolytic carbon was provided by Department of Biological Material of Sichuan Union University;staphylococcus anreus,Escherichia coli,staphylococcus epidermidis,and Pseudomonas aerugmosa were prepared by our laboratory.METHODS:The growth curve of staphylococcus aureus,staphylococcus epidermidis,Escherichia coli and Pseudomonas aeruginosa on dacron,pyrolytic carbon and pelytetrafluoroethylene were quantitatively determined respectively by plate counting and gamma.ray counting of 125 Ⅰ radiolabeled bacteria in vitro.Bacteria growing normally served as control.All bacteria were cultured for 30 hours,and bacteria concentration was determined every 2 hours.In addition,the adhesive capacities of foMr kinds of bacteria on dacron,pyrolytic carbon and polytetrafluoroethylene were detected Escherichia coli and Pseudomonas aeruginosa on dacron,pyrolytic carbon and polytetrafluoroethytene.RESULTS:There were no significant differences in adhesive capacities of each bacterium on dacron,pyrolytic carbon and polytetrafluoroethylene at the same time point(P>0.05).The differences in growth curve of four kinds of bacteria on prosthetic valve materials were not remarkable compared to the control(P>0.05).Different bacteria showed different adhesion degree on the materials:staphylococcus aureus exhibited strongest adhesion on dacron;staphylococcus epidermidis on pyrolytic carbon;Escherichia coli on dacron.The adhesive capacity of Pseudomonas aerugmosa on dacron reached peak within 12 hours,and gradually decreased,but maintained strong adhesion on the other materials.The adhesive capacmes of four bacteria on the materials did not increase or maintain with time.CONCLUSION:The adhesive capacity of one bacterium to different artificial valve materials and different bacteria to one prosthetic valve materials is different.The materials.show little influence on bacterium growth cycle.

Objective To evaluate the association of c-erbB-2 protein expression with the prognosis of gastric carcinoma. Methods Eleven literatures were analyzed which published in recent 15 years on the relationship of c-erbB-2 with prognosis quantitively by software RevMan. Results The pooled odds ratio(OR) of 5-year survival rates was 0.69(95% CI:0.54～0.87)for all 11 literatures. The pooled odds ratio(OR) of three literatures from eleven literatures was 0.67(95%CI:0.48～0.94)which 5-year survival rates was determined as final index and OR as associated.For well-differentiated and advanced gastric carcinoma, the pooled odds ratio(OR) of 5～year survival rates were 0.18(95%CI:0.09～0.38)and 0.72(95%CI:0.47～1.09)respectively.Conclusion The association of c-erbB-2 expression with prognosis of gastric carcinoma is negative.