ObjectiveTo investigate the effect of high volume hemofiltration (HVHF) combined with mechanical ventilation (MV) on seawater respiratory distress syndrome (SW-RDS) canine models.MethodsTen nomal hybrid dogs were randomly assigned into two groups:MV group(MV group,n=5),all the animals only received MV after establishing model successfully; HVHF combined with MV group (HVHF+MV group,n=5),all were received HVHF plus MV after establishing model successfully.Both groups were observed for 4 hours.Mean arterial pressure (MAP),heart rate (HR),central venous pressure(CVP),arterial blood gas and venous plasma osmotic pressure were detected at baseline,0 min(model establishment),60 min,120 min,180 min,240min after treatment.Venous blood was collected to detect inflammatory mediators (IL-8,IL-6,TNF-α)at baseline,0 min,120 min,240 min after treatment.The lung pathology was examined at the end of the experiment.Results(1)All the animals were suvival after four hous of treatment in both groups. (2)Pattial pressure ofoxygen(PaO2) and O2 saturation(SaO2) rised after four hours of treatment in both groups(P＜0.05), and HVHF+MV group was better than MV group.After 4 hours of treatment,pH,actual bicarbonate (AB),bases excess(BE) in HVHF+MV group were significantly better than those in MV group(P＜0.05),recovering to the baseline values.(3)MAP,HR,CVP were stable during the four hours of treatment,and compared with 0 min,there was no significant differences after 4 hours of treatment in bothgroups. There were no significant differemces at the same time of treatment in both groups. (4)Plasma osmotic pressure were stable during the four hours of treatment,and compared with 0 min,there was no significant difference in MV group.But in HVHF+MV group,osmotic pressure was significantly higher after 4 hours of treatment than that at the same time in MV group(P＜0.05),and compared with 0 min and 180 min,those were higher too(P＜0.01). (5)Compared with those at the same time in MV group,plasma inflammatory mediators (IL-8,IL-6,TNF-α) were significantly decreased after 4 hours of treatment in HVHF+MV group(P＜0.01).After 4 hours of treatment IL-8,TNF-α in MV group were higher than those at the same time in 0 min (P＜0.05).(6)Compared with those in MV group,there were less infiltration of neutrophils,edema and injury of alveolar epithelium from pulmonary pathology.ConclusionsHVHF combined with MV can significantly improve hypoxemia and correct acidosis of SW-RDS model in canines.HVHF can effectively clear plasma inflammatory mediators and redundant water,improve pulmonary pathology changes.HVHF has no impact on MAP,HR and CVP of SW-RDS.

Recently, more and more public attention has been paid to nanomaterials in various fields. Especially, the preparation methods of core/shell nanoparticles have been drastically updated and developed. There exists great application prospect for the development of biosensing core/shell nanoparticles. This paper emphatically introduced the operation principle, preparation methods of biosensing core/shell nanopaticles and the latest application progress in electrochemical biosensor, optical biosensor and piezoelectric crystal biosensor.

Objective To explore the underlying anatomy of iliac vein compression syndrome (IVCS) using CT, and discuss the imageological index for subtype diagnosis and potential clinical significance. Methods We retrospectively analyzed the imageological data of 69 IVCS patients from July, 2009 to June, 2014. According to CT findings, patients were categorized to simple IVCS (sIVCS, the iliac vein is compressed by only the anterior iliac artery, n=22), lumbar degeneration?related IVCS ( dIVCS, besides the iliac artery, the lower lumbar degenerative changes including osteophyte, protruded disc, etc. pressed the iliac vein from the back, n=33) and other IVCS causes (oIVCS, including tuberculosis, inflammation and fracture hematoma of the lumbar spine, n=14); meanwhile, 69 age? and sex?matched subjects was set as a control group. Evaluated indexes were onset age, course of lower limb swelling and pain, size of the iliac vein tunnel ahead lumbar (IVTAL), lower lumbar spine forward curvature angle (LLSCA), iliac vein?pressed signs by lower lumbar degeneration, compression sites, scope of deep venous thrombosis and interventional therapeutic effect. The differences of those indexes of various groups were compared. Data as onset age, course of disease, sizes of IVTAL and LLSCA were evaluated using variance analysis of the general linear model, with Bofferroni test correction for multiple comparisons. Data as iliac vein?pressed site, scope of venous embolism and therapeutic effect were assessed by crossing table χ2 test. All statistical analyses were performed using SPSS17.0 software (SPSS company, Chicago, USA). Bilateral P≤0.05 were considered to be significant. Results The onset mean age was (61.5 ± 10.6) yr. in dIVCS, (42.3 ± 6.5)yr in sIVCS. and(53.1 ± 16.8)yr. in oIVCS with a significant difference ( F=11.030, P<0.01). Mean sagittal diameter of the IVTAL and LLSCA were(2.3±0.5)mm and(121.8±5.4)° in dIVCS and(2.5± 0.5)mm and(124.4 ± 3.9)° in sIVCS, respectively; which were smaller than those of control group [(6.4 ± 1.6)mm and(127.5 ± 7.3)° , respectively ] and oIVCS [(5.9 ± 2.3)mm and(129.5 ± 5.9)° , respectively ](F=125.275,P<0.01 for sigittal diameter and F=7.95,P<0.01 for LLSCA). The degenerative changes compressing the iliac vein of dIVCS had 41 sites of 33 patients which were respectively the discal forward protrusion or bulge (51%, 17/33), vertebral anterior osteophyte (50%, 16/33)and lower lumbar vertebrae slippage (19%,8/33). The compression sites of dIVCS located exactly anterior to the fifth lumbar(18/33, 56%), the intervertebral disk between the fourth and fifth lumbar(9/33, 26%), the right front (3/33, 9%) and left front (3/33, 9%) of the fifth lumbar, however, that of sIVCS sited the right front(14/22, 64%), exactly the front(4/22, 18%)of the fifth lumbar and exactly anterior(4/22, 18%)to the intervertebral disk between the fourth and fifth lumbar, with a significant difference of location distribution(χ2=19.305, P<0.01). In sIVCSs, deep vein thrombosis of all exceeded the iliac vein length and implicated the femoral vein by 27%(6/22)and the popliteal vein by 73%(16/22), while in dIVCSs 18%(6/33)patients had deep vein thrombosis in the iliac vein, 30%(10/33)in the femoral vein and 52%(17/33)in the popliteal vein, and in oIVCSs, 30%(4/14)patients had deep vein thrombosis in the iliac vein, 40%(6/14)in the femoral vein and 30%(4/14)in the popliteal vein, with a significant difference of thrombosis scope(χ2=9.28, P<0.01). 86%sIVCSs needed intravenous stent?implanted operation to obtain effective treatment, only 52%dIVCSs were performed stent?implanted operation for effective therapy, none of oIVCSs had stent?implanted operation before the iliac vein recanalization. Conclusion CT can precisely display the pathological anatomy features of different IVCS patients, which can potentially help clinicians plan accurate treatment strategy.

Objective To evaluate the effects of Acorustatarinowii Schott(ATS)on chronic restraint stress mice and its possible mechanisms. Methods Chronic stressed mice underwent a repeated 8h/d constraint in a specific behavior blocker ,however ,only CSA mice received once gavages of 4.5 g/(kg · d)ATS decoction for con-secutive 28 days. Morris water maze(MWM)task was conducted for evaluating learning and memory of the mice. ELISA was used to examine the levels of plasma corticosteroid. Results ATS dramatically ameliorated cognitive impairments and decreased serum corticosteroid level in the stressed mice(P<0.01). Conclusion ATS improves cognitive deficits provoked by chronic stress in mice ,which may attribute to decreasing plasma corticosteroid levels.

Objective To analyze the influencing factors of AVM bleeding for surgical treatment.Methods The 64 cases of bleeding AVM patients,clinical informations,condition of surgery and inspection of pathology were summarized.Results The prognosis was evaluated by Glasgow outcome scale(GOS)for every patient after the operation.36(53.3 %)recovered well,13(20.3 %)moderately disabled,4(6.4 %)severely disabled,and 11(17.3 %)died.Conclusions The effect of surgical treatment in patients with the age of onset,the site of hematoma,the bleeding volume and timing of surgery-related.The incidence between the ages of 8～14 years of age,the amount of bleeding

It elaborates streaming media technology and the principle of it's work, introduces the selection of streaming media system and recounted the constitute of the Windows Media Technology system and the method of making medical teaching lesson pieces using streaming media technique. At last, it discusses the application in the LAN.

Objective To analyze causes for postoperative coxa vara and anti-rotation nail cutting-out after treatment of brittle femoral intertrochanteric fractures with proximal femoral nails ( PFN ).Methods An retrospective study was done on 227 patients with intertrochanteric fracture treated with PFN from June 2006 to February 2009. The causes for postoperative coxa vara and anti-rotation nail cutting-out were analyzed. Harris score was used to evaluate the functional recovery of the hip joint. Results Of all, 221 patients were followed up for 12-48 months (mean 23 months) and six patients were died from serious internal disease within one year. According to Harris evaluation system, the results were excellent and good in 183 patients, fair in 30 and poor in 14. Postoperative coxa vara and anti-rotation nail cuttingout occurred in 16 patients, eight of whom received reoperation to remove internal fixation and skeletal traction at abducent position and the other eight received prosthetic replacement. Conclusions Treatment of proximal femoral fracture with PFN requires a high precision of reduction and operation. Many factors including lateral cortical bone conditions of tuberosity, postoperative patient's cognitive condition,use of improved Jensen-Evans classification and Singh's classification may affect operation outcome.

To determinate the chikusetsusaponin IVa in Rhizoma Panacis Majoris from different producing areas. The HPLC separation was performed on a Inertsil ODS-sp column (4.6 mm x 150 mm, 5 microm). A mixture of acetonitrile and 0.2% phosphoric acid solution (35:65) as the mobile phase the column. Temperature was set in 30 degrees C. The flow rate was 1.0 mL x min(-1), and the wave length of the detector is 203 nm. The content of the chikusetsusaponin IVa in Rhizoma Panacis Majoris from Meixian, Shaanxi is the highest and the lowest is from Enshi, Hubei. There have most differerence among the content of the chikusetsusaponin IVa in Rhizoma Panacis Majoris from different producing areas.
Chromatography, High Pressure Liquid ; Linear Models ; Oleanolic Acid ; analogs & derivatives ; analysis ; isolation & purification ; Panax ; chemistry ; Reproducibility of Results ; Rhizome ; chemistry ; Saponins ; analysis ; isolation & purification

Objective To investigate the effect of CCK-8 on CHOP/caspase-11/caspase-1 expressions induced by LPS in RAW264.7 cells.Methods RAW264.7 cells were incubated with LPS and/or CCK-8 and/or CCK-8 1R blocking agent devazepide for indicated times .CHOP and caspase-11 protein expressions were determined by West-ern blot , caspase-1 activity was analyzed by kit , IL-1βexpression level was analyzed by ELISA .Results Both CHOP and caspase-11 expression were upregulated in LPS-activated RAW264.7 cell, which were inhibited by pre-treatment of CCK-8 .Pre-treatment of devazepide inhibited the effects of CCK-8 significantly .The same effects were found in caspase-1 activity and IL-1βexpression .Conclusions CCK-8 exerts an anti-inflammatory effect by inhib-iting CHOP/caspase-11/caspase-1 expressions induced by LPS in RAW264.7 cell.CCK 1R may contribute to the anti-inflammatory effect of CCK-8.

Objective: To investigate the multivoxel proton MR spectroscopy findings of brain tumors and the clinical value in differential diagnosis. Methods:Sixty patients with brain tumors underwent multivoxel proton MR spectroscopic examination with PRESS sequence. Distribution of the 60 final diagnoses of neoplasms was as follow: meningioma (n=22), glioma (n=21), lymphoma (n=3), metastasis (n=5), acoustic neuroma, pituitary tumor, PNET (n=2, respectively), vascularblastoma, arachnoid cyst, neurofibromatosis (n=1,respectively). The concentrations of NAA, Cho, Cr, Lac-Lip were obtained in the tumors and the contralateral normal brain region. The ratios of NAA/Cr, Cho/Cr, NAA/Cho, tumor NAA/normal NAA, tumor Cho/normal Cho, tumor Cr/normal Cr were calculated. Results: 1H MRS showed decreased NAA, Cr and elevated Cho. NAA concentrations in meningioma, glioma, lymphoma decreased significantly to acoustic neuroma, metastasis, but Cho in tumors changed without significance. NAA/Cr ratio was significantly lower in glioma than in acoustic neuroma and metastasis, and p value was 0.014, 0.027, respectively. No significance was found in tumor Cho/Cr data. Conclusion:Multivoxel proton MR spectroscopy is available for study of tumor metabolites. The tumor NAA/normal NAA, NAA/Cr were helpful for the diagnosis and differential diagnosis of intracranial tumors.