Objective To observe the behavior of Schwann cell migration and wrapping up of fibres during co-culture of Schwann cells with steroframe of PLA and PLGA fibres. Methods Schwann cells were mixed into 30% ECM gel and co-cultured with PLA non-spinning fibre steroframes and seeded on one ends of PLGA fibres which were treated with collagen,polylysine or ECM. The attachment,growth and migration of Schwann cells were observed under phase contrast microscope and laser scan confocus microscope. Results (1)When Schwann cells were co-cultured with PLA steroframes,most of the Schwann cells kept in the fibre holes and attached on PLA fibres to form B?ngner band likd structures. (2)When co-cultured with PLGA fibers,Schwann cells could attach and migrate on PLGA fibres and the number of Schwann cells were obviously increased when PLGA fibres were treated by ECM. Conclusions (1)ECM gel colud promote Schwann cells attachment,growth and migration on PLA steroframes and PLGA fibers. (2)ECM gel is a good integrating substance for constructing tissue engineering bioartificial nerve.

0.05) . [Conclusion] RAI has certain therapeutic effect on peripheral blood leukopenia caused by viral infection in children.

Objective To investigate the methods and effect of the dual incision lock type golf combined with support plate in the treatment of tibial plateau fracture.Methods 80 cases with comminuted fracture of the tibial plateau were randomly divided into two groups.40 cases in the observation group were given the dual incision lock type golf combined with support plate.and the control group used traditional single incision T-shaped steel treatment.The clinical efficacy was compared between the two groups.Results 80 patients were followed up.Excellent rate of the observation group was 67.5％,which was significantly higher than 45.0％ of the control group.The incidence rate of complication in observation group was 2.5 ％,which was significantly lower than 22.5 ％ in the control group (P ＜ 0.05).The reset extent of patients,healing time,loading time,HSS score of the observation group were significantly better than those of the control group (P ＜ 0.05).Conclusion The dual incision lock type golf combined with support plate in the treatment of tibial plateau fracture has less trauma,reliable fixation,early functional exercise andfewer complications.

With the continuous improvement and innovation of modern engineering technology and biomedical technology,many efforts and process have been made on multifunctional prosthesis systems and have gained more and more attentions.The multifunctional powered prosthesis has become one of the research areas in the current muscular rehabilitation medicine and neural engineering.The bionic control of prosthesis,especially using the neural signal to control the upper prosthesis effectively,is one of the most important fields of prosthesis technology.Choosing the proper neural signals seems to be particularly important for bionic prosthetic control strategy.Currently,several different signal controls such as EMG control,EEG control,voice signal control and peripheral nerve signal control have been proposed and investigated for the bionic control of multi-function prosthesis.This paper reviews the latest efforts and progress of multifunctional upper prosthesis.

Objective To evaluate the techniques and efficacy of artery embolization in the treatment of benign prostatic hyperplasia(BHP).Methods This study included 12 patients(age range,61-82 years) who were diagnosed to have BHP by clinical manifestations,CT and B-ultrasound,with the disease course of 2-16 years.The relevant parameters were as follows:mean residual urine(RU) of 138 ml,Qmax of 9.6 ml/s,mean IPSS of 24.2 and QOL of 4.8.In these patients,the prostate blood-supply arteries were confirmed by super-selective arteriography,and were embolized by injection of PVA and Gelfoam via artery catheters.The pre-and post-operative IPSS,QOL,Qmax and RU were compared.Meanwhile the changes of prostate volume and blood supply were evaluated by CT and B-ultrasound.In addition,the change of urethral diameter was evaluated by urethrography.Results In the 12 patients,21 prostate blood-supply arteries were embolized,including 5 branches of internal iliac artery,9 branches of inferior vesical artery,5 branches of internal pudendal artery,2 branches of obturator artery.The arteries were embolized bilaterally in 9 patients and embolized unilaterally in 3 patients.Postoperatively,the mean IPSS was 4.8;QOL,1.3;Qmax,18.9 ml/s;RU,0-3 ml,which indicated that the urethral obstruction was obviously improved after operation.CT and B-ultrasound showed that the prostate volume was obviously decreased from 127 ml to 90 ml on average with a reduction rate of 71%,and the urethral stricture disappeared on X-ray examination.Color Doppler imaging showed that the blood supply inside the prostate was reduced.Conclusions The artery embolization for the treatment of BHP is a new method with several virtues of less trauma, marked effect,better safety and fewer complications.

Objective To investigate the relations between low birth weight and infantile cerebral palsy. Methods Population based survey was done in townships in Nanning city, Heng county and northern district of Qinzhou city from May to December in 1998. All children in the project townships, from 1 to 6 years old, was investigated for cerebral palsy(CCP). The pediatricians were well trained and qualified before participated. Results One hundred and fifty thousand eight hundred and six infants were enrolled, and 193 infants with cerebral palsy were diagnosed, and the morality of CP was 1.28‰. Sixty one infants with cerebral palsy were found among 1 450 low birth weight infants(42.07‰. One hundred and twenty five cerebral palsy infant were diagnosed among 148 599 infants with normal birth weight(0.84‰). The cerebral palsy rates were significantly different between low birth weight and normal birth weight infants. The lower the birth weight was, the higher the morality. Among the 61 cases of the cerebral palsy infants with low birth weight, there were different conditions of the mothers in the aspects of environment and nutrition during their pregnant period. Among these mothers, 1/3 of them had been got the unhealthy factors;26 cases of them (42.6%) had had complications of pregnancy;38 cases of them(62.3%)had been ill during the neonatal period;20 case of them(32.8%) had been got the abnormal pregnant and delivering history or the premature labor history. Conclusions The low birth weight is a highly risk factor related to cerebral palsy, the mothers have similar disease in the pregnant period. Low birth weight infants should get intensive care, and the illness neonates should be prevented.

ObjectiveTo study the relation between premature and cerebral palsy(CP).MethodsTo sample all the children between the age of 1 to 6 years old for investigating CP situation in Nanning city, Heng county and Qinbei region of Qinzhou city (once named Qinzhou county ) from May to December, 1998 by qualified pediatricians.ResultsThe total investigating cases were 150,806,in which diagnosed CP were 193 cases and the incidence of CP was 1.28‰. Among them, premature infants were 872 and diagnosed CP were 46,the incidence of CP was 52.75‰ in this group. While term laboring infants were 149,934 with CP 142 and the incidence of CP was 0.95‰. The difference between two groups was very significant(P<0.001).The shorter the gestational weeks were, the higher the incidence of the CP was. Among 46 premature infants with CP, there were 18 cases' mothers(26 person times) involved in abnormal pregnancy and delivery history, accounted for 39.1%, while 36 with pregnancy complications(78.3%). There were 23 cases (30 person times) suffered from the disease during neonate, accounted for 50%.ConclusionThe premature delivery is a risk factor associated with childhood CP.

Objective To evaluate the cLinical outcome and influencing factors of open reduction and internal fixation in treatment of hip dislocation combined with acetabular fractures. Methods A retrospective analysis was performed on 51 patients with hip dislocation combined with twetabular frac-tures, who were treated with open reduction and internal fixation under general anesthesia in the emergen-cy department on admission. Of all, 41 patients were treated with open reduction and plate/screw internal fixation, for which the reduction result was evaluated by postoperative X-rays and follow up X-rays accord-ing to Matta's criteria and the functional outcome by Merle d' Aubigne's criteria. Results Of 41 pa-tients, 33 were followed up for 1-7 years (mean 3.1 years). X-ray evaluation showed anatomic reduction in 27 patients (82%), imperfect reduction in five (15%) and poor reduction in one (3%). The clini-cal outcome at the time of final follow-up was graded as excellent in 18 patients (55%), good in 8 (24%), mederate in 3 (9%) and peor in4 (12%), with total excellence rate of 79%. Conclusion Prompt reduction of hip dislocation, precise reduction of the acetabular fracture and decrease of periopera-tive comphcations are key to excellent clinical outcome.

Objective To evaluate the clinical results of minimally invasive plate osteosynthesis (MIPO) in treatment of complex fractures of the distal femur. Methads From July 2005 to December 2006, 41 patients with complex distal femur fractures induced by high energy trauma were treated with MIPO plus locking compression plate(LCP). There were 31 males and 10 females, at average age of 38 years(17-65 years). According to the AO/OTA fracture classification system, there were 11 patients with type A3 fractures, 22 with type C2 and 8 with type C3. There were 16 patients with open fractures. The clinical result was evaluated by using function examination and radiographic assessment. Results All patients were followed up for average 8 months(2-18 months), which showed fracture healing, with mean healing time of 13 weeks(7. 16 weeks). There was only 1 patient with superficial infection but no fixation failure or implant breakage were found. The average knee range of motion was 110°(45°-135°). Hospital for special surgery knee rating scale(HSS) showed excellent or good results in 36 patients (88%). No patient with greater than 5°difference in either varus or valgus was detected when compared 2 days after operation with latest follow-up. Conclusion In treatment of complex distal femur frac-tures, MIPO plus LCP have advantages of minor trauma, stable fLxation, slight interference on soft tis-sues, early fracture healing and few complications.

Breeding and quality control of SPF nude mice involves many links and works .The quality and quantity of animals are directly affected by breeding management and quality control .A standardized breeding system of SPF BALB/c nude mice were gradually established at the center after 2007.This paper introduces specific practices and experience in facilities, stock, breeding management , and quality control of SPF BALB/c nude mice, in order to provide the reference for related breeding and study in SPF BALB/c nude mice.