Objective To observe the behavior of Schwann cell migration and wrapping up of fibres during co-culture of Schwann cells with steroframe of PLA and PLGA fibres. Methods Schwann cells were mixed into 30% ECM gel and co-cultured with PLA non-spinning fibre steroframes and seeded on one ends of PLGA fibres which were treated with collagen,polylysine or ECM. The attachment,growth and migration of Schwann cells were observed under phase contrast microscope and laser scan confocus microscope. Results (1)When Schwann cells were co-cultured with PLA steroframes,most of the Schwann cells kept in the fibre holes and attached on PLA fibres to form B?ngner band likd structures. (2)When co-cultured with PLGA fibers,Schwann cells could attach and migrate on PLGA fibres and the number of Schwann cells were obviously increased when PLGA fibres were treated by ECM. Conclusions (1)ECM gel colud promote Schwann cells attachment,growth and migration on PLA steroframes and PLGA fibers. (2)ECM gel is a good integrating substance for constructing tissue engineering bioartificial nerve.

0.05) . [Conclusion] RAI has certain therapeutic effect on peripheral blood leukopenia caused by viral infection in children.

Objective To investigate the methods and effect of the dual incision lock type golf combined with support plate in the treatment of tibial plateau fracture.Methods 80 cases with comminuted fracture of the tibial plateau were randomly divided into two groups.40 cases in the observation group were given the dual incision lock type golf combined with support plate.and the control group used traditional single incision T-shaped steel treatment.The clinical efficacy was compared between the two groups.Results 80 patients were followed up.Excellent rate of the observation group was 67.5％,which was significantly higher than 45.0％ of the control group.The incidence rate of complication in observation group was 2.5 ％,which was significantly lower than 22.5 ％ in the control group (P ＜ 0.05).The reset extent of patients,healing time,loading time,HSS score of the observation group were significantly better than those of the control group (P ＜ 0.05).Conclusion The dual incision lock type golf combined with support plate in the treatment of tibial plateau fracture has less trauma,reliable fixation,early functional exercise andfewer complications.

Objective To evaluate the techniques and efficacy of artery embolization in the treatment of benign prostatic hyperplasia(BHP).Methods This study included 12 patients(age range,61-82 years) who were diagnosed to have BHP by clinical manifestations,CT and B-ultrasound,with the disease course of 2-16 years.The relevant parameters were as follows:mean residual urine(RU) of 138 ml,Qmax of 9.6 ml/s,mean IPSS of 24.2 and QOL of 4.8.In these patients,the prostate blood-supply arteries were confirmed by super-selective arteriography,and were embolized by injection of PVA and Gelfoam via artery catheters.The pre-and post-operative IPSS,QOL,Qmax and RU were compared.Meanwhile the changes of prostate volume and blood supply were evaluated by CT and B-ultrasound.In addition,the change of urethral diameter was evaluated by urethrography.Results In the 12 patients,21 prostate blood-supply arteries were embolized,including 5 branches of internal iliac artery,9 branches of inferior vesical artery,5 branches of internal pudendal artery,2 branches of obturator artery.The arteries were embolized bilaterally in 9 patients and embolized unilaterally in 3 patients.Postoperatively,the mean IPSS was 4.8;QOL,1.3;Qmax,18.9 ml/s;RU,0-3 ml,which indicated that the urethral obstruction was obviously improved after operation.CT and B-ultrasound showed that the prostate volume was obviously decreased from 127 ml to 90 ml on average with a reduction rate of 71%,and the urethral stricture disappeared on X-ray examination.Color Doppler imaging showed that the blood supply inside the prostate was reduced.Conclusions The artery embolization for the treatment of BHP is a new method with several virtues of less trauma, marked effect,better safety and fewer complications.

With the continuous improvement and innovation of modern engineering technology and biomedical technology,many efforts and process have been made on multifunctional prosthesis systems and have gained more and more attentions.The multifunctional powered prosthesis has become one of the research areas in the current muscular rehabilitation medicine and neural engineering.The bionic control of prosthesis,especially using the neural signal to control the upper prosthesis effectively,is one of the most important fields of prosthesis technology.Choosing the proper neural signals seems to be particularly important for bionic prosthetic control strategy.Currently,several different signal controls such as EMG control,EEG control,voice signal control and peripheral nerve signal control have been proposed and investigated for the bionic control of multi-function prosthesis.This paper reviews the latest efforts and progress of multifunctional upper prosthesis.

ObjectiveTo study the relation between premature and cerebral palsy(CP).MethodsTo sample all the children between the age of 1 to 6 years old for investigating CP situation in Nanning city, Heng county and Qinbei region of Qinzhou city (once named Qinzhou county ) from May to December, 1998 by qualified pediatricians.ResultsThe total investigating cases were 150,806,in which diagnosed CP were 193 cases and the incidence of CP was 1.28‰. Among them, premature infants were 872 and diagnosed CP were 46,the incidence of CP was 52.75‰ in this group. While term laboring infants were 149,934 with CP 142 and the incidence of CP was 0.95‰. The difference between two groups was very significant(P<0.001).The shorter the gestational weeks were, the higher the incidence of the CP was. Among 46 premature infants with CP, there were 18 cases' mothers(26 person times) involved in abnormal pregnancy and delivery history, accounted for 39.1%, while 36 with pregnancy complications(78.3%). There were 23 cases (30 person times) suffered from the disease during neonate, accounted for 50%.ConclusionThe premature delivery is a risk factor associated with childhood CP.

Objective To investigate the relations between low birth weight and infantile cerebral palsy. Methods Population based survey was done in townships in Nanning city, Heng county and northern district of Qinzhou city from May to December in 1998. All children in the project townships, from 1 to 6 years old, was investigated for cerebral palsy(CCP). The pediatricians were well trained and qualified before participated. Results One hundred and fifty thousand eight hundred and six infants were enrolled, and 193 infants with cerebral palsy were diagnosed, and the morality of CP was 1.28‰. Sixty one infants with cerebral palsy were found among 1 450 low birth weight infants(42.07‰. One hundred and twenty five cerebral palsy infant were diagnosed among 148 599 infants with normal birth weight(0.84‰). The cerebral palsy rates were significantly different between low birth weight and normal birth weight infants. The lower the birth weight was, the higher the morality. Among the 61 cases of the cerebral palsy infants with low birth weight, there were different conditions of the mothers in the aspects of environment and nutrition during their pregnant period. Among these mothers, 1/3 of them had been got the unhealthy factors;26 cases of them (42.6%) had had complications of pregnancy;38 cases of them(62.3%)had been ill during the neonatal period;20 case of them(32.8%) had been got the abnormal pregnant and delivering history or the premature labor history. Conclusions The low birth weight is a highly risk factor related to cerebral palsy, the mothers have similar disease in the pregnant period. Low birth weight infants should get intensive care, and the illness neonates should be prevented.

Objective To quantitatively analyze the fund theses published in the Journal of Practical Radiology(JPR)and Chinese Journal of Radiology(CJR)in order to detect effective approaches to the improvement of the journal quality and academic level of the JPR.Methods The number of funds-supported theses published,the ratio of fund theses,grade,regional,source of institutions and publication time-lag distribution of the fund theses were statistically analyzed by using bibliometrics in the two journals in 2013.Re-sults Six hundred and eighteen theses were published in JPR in 2013,the total number of fund theses were 137(accounted for 22.2% of total articles),the ratio of fund theses were 0.22,the provincial and municipal fund theses accounted for 59.2%.All fund theses were from 23 regions and 82 institutions throughout the country.The average of publication time-lag was 235.6 days.Three hundred and thirteen theses were published in CJR in 2013,the total number of fund theses were 97(accounted for 31.0% of total articles),the ratio of fund theses were 0.31,the provincial and municipal fund theses accounted for 32.0%.All fund theses were from 18 regions and 70 institutions throughout the country.The average of publication time-lag was 228.4 days.Conclusion JPR has its own superi-ority,meanwhile,it also has a significant disparity and insufficiency comparing to CJR.In order to further promote the quality conno-tations and the academic level of JPR,efforts should be made on subject selection and planning,initiative collection of manuscripts, priority publishment to excellent manuscripts,shortening publication time-lag,etc.Additionally,a database of core authors and ex-perts should be established.

Breeding and quality control of SPF nude mice involves many links and works .The quality and quantity of animals are directly affected by breeding management and quality control .A standardized breeding system of SPF BALB/c nude mice were gradually established at the center after 2007.This paper introduces specific practices and experience in facilities, stock, breeding management , and quality control of SPF BALB/c nude mice, in order to provide the reference for related breeding and study in SPF BALB/c nude mice.

BACKGROUND:Studies have shown that umbilical cord blood stem cel transplantation promote the recovery of spinal cord injury, and electroacupuncture also can inhibit the proliferation of astrocytes to reduce damage to scar formation, suggesting that a combination of umbilical cord blood stem cel transplantation and electroacupuncture may play an important role in the treatment of acute spinal cord injuries. OBJECTIVE:To observe the influence of transplantation of human umbilical cord blood stem cels combined with electroacupuncture at theDu channel on expression of nerve growth factor and neurotrophin 3 in rats with spinal cord injuries. METHODS: Seventy-two female Sprague-Dawlay rats were randomly divided into control group, injury group, transplantation group and combined therapy group. In the control group, only an incision on the back was sutured;in the injury group, a piece of saline-infiltrated gelatin sponge, 1 mm×2 mm×2 mm, was placed into the transected spinal cord at T10 level; in the transplantation group and combined therapy group, a piece of gelatin sponged infiltrated in the suspension of human umbilical cord blood stem cels was placed into the transected spinal cord, respectively, and then, electroacupuncture stimulation at the Duchannel was performed in the combined therapy group at 1 hour after modeling. Specimens were taken at 7, 14, 28 days after modeling in each group, and then immunohistochemistry, western blot and real time-PCR methods were used to detect the expression of nerve growth factor and neurotrophin 3. RESULTS AND CONCLUSION:Compared with the transplantation group, the expression of nerve growth factor and neurotrophin 3 was lower in the injury group but higher in the combined therapy group at 7, 14, 28 days after modeling (P < 0.05). The results of western blot and real time-PCR were consistent with those of immunohistochemical detection. Findings show that human umbilical cord blood stem cel transplantation combined with electroacupuncture has a remarkable synergistic effect in the treatment of spinal cord injury that can significantly up-regulate the expression of nerve growth factor and neurotrophin 3, and contribute to injured spinal cord repair, regeneration and functional recovery after spinal cord injury.