OBJECTIVE: To investigate the pathological factors of syndrome pathomechanism through studying the correlation between syndromes of posthepatitic cirrhosis and biological parameters. METHODS: Clinical information of three hundred and fifty-five patients with posthepatitic cirrhosis was collected and the database was established. Parameters with statistical significance were analyzed with multi-factor regression analysis to investigate the main influencing factors of the syndromes of posthepatitic cirrhosis. RESULTS: Formulae of six syndromes, including syndrome of yin deficiency of liver and kidney, syndrome of damp heat, syndrome of stagnated heat smoldering, syndrome of yang deficiency of spleen and kidney, syndrome of stagnation of liver qi and spleen deficiency and syndrome of blood stasis due to qi deficiency, were established with stepwise regression analysis. CONCLUSIONS: One of the pathophysiological bases of syndrome of blood stasis due to qi deficiency in cirrhosis is synthetic dysfunction of hepatocytes. The pathophysiological basis of syndrome of damp heat is inflammatory injury, which is also syndrome of stagnated heat smoldering in cirrhosis patients. The relationship between syndrome of yin deficiency of liver and kidney and stasis and damp heat may be the pathophysiological basis of the posthepatitic cirrhosis..

The influence of Cibotium barometz (L.) J. Sm. and its processed samples on thrombin-induced platelet aggregation in rabbits was studied. The results showed that all differently processed sam-ples tested could inhibit platelet aggregation, with activities in the decreasing order of Rhizoma Cibotiiroasted in stirring sand＞steamed after being salted＞steamed after steeped in wine＞simply steamed＞theunprocessed crude Rhizoma Cibotii.

Objectives:To compare the advantage and the disadvantage between the normally inserted central venous catheter(NICC) and the peripherally inserted central venous catheter(PICC). Methods:The patients were divided into two groups by different insertion methods:NICC group and PICC group.The success rate of first insertion point,the time of insertion, the symptom of patients during insertion, the complication of insertion and the rate of catheter septicemia were compared. Results:The group of PICC had a higher rate of successful insertion, a less time of insertion, a less time of insertion, a lower rate of complication, but a higher rate of catheter septicemia,when compared with the group of NICC. Conclusions:①PICC have some advantages being simple in insertion and of less complications.②The PICC patients have more chances to suffer from the catheter septicemia.③Perepheral venous inflammation and veno-occlusion more earily occurr in PICC patients.

OBJECTIVETo investigate the effect of Fuzheng Huayu decoction (FHD) intervention on hepatic fibrosis.

METHODSWistar rats were randomly divided into 3 groups: rats in the normal group only treated with subcutaneous injection of saline, rats in the model group and the FHD group were made into hepatic fibrosis by subcutaneous injection of 40% carbon tetrachloride (CCl4)-olive solution and then those in the FHD group were treated with FHD by gastric perfusion after modeling. Liver samples of the rats were obtained for routine pathological observation, hydroxyproline determination and proteome quantitative determination. After then, the proteome profile was obtained through 2-dimensional electrophoresis and silver staining, and analyzed. More than 30 proteins with different expression were identified by MALDI-TOF-MS.

RESULTS(1) The integral response of vital movement such as body weight and activity of hepatic fibrosis declined in the CCl4 induced liver fibrosis rats; (2) Liver fibrosis were associated with abnormal metabolism; (3) There were four material metabolism-related protein showed by hepatic proteome mass spectrography, which expressed different between the normal and the fibrotic rats, i. e. the perchloric acid soluble protein, the phosphatidylinositol transferase, the phosphoglycerate kinase and the endoplasmic reticulum-60 protease; (4) The expressions of the above-mentioned four proteins in the FHD group were nearly the same as those of normal level.

CONCLUSION(1) Liver fibrosis is accompanied with abnormal protein synthesis and decomposition, as well as the enhanced activity of glycolysis; (2) The existence of metabolism-related proteins is one of the elements for the liver in regulating metabolism; (3) The regulation on the expressions of metablism-related proteins is one of the pathways for FHD to exert its anti-hepatic fibrosis effect.

Animals ; Carbon Tetrachloride ; Drugs, Chinese Herbal ; therapeutic use ; Hydroxyproline ; metabolism ; Liver ; metabolism ; Liver Cirrhosis, Experimental ; drug therapy ; metabolism ; Male ; Phosphoglycerate Kinase ; metabolism ; Phytotherapy ; Proteins ; metabolism ; Random Allocation ; Rats ; Rats, Wistar

ObjectiveTo explore the mechanism of Shangerhuang Wan （SEHW） and its subdivisions in alleviating loperamide-induced functional constipation （FC） in rats by regulating bile acid metabolism. MethodsSixty rats were randomly assigned into six groups （n=10）： normal control， model， positive control （domperidone， 8 mg·kg^-1）， Cimicifugae Rhizoma-Bupleuri Radix （SC， 1.33 g·kg^-1）， Scutellariae Radix-Coptidis Rhizoma （QL， 1.20 g·kg^-1）， and SEHW （3.33 g·kg^-1）. The remaining groups except the normal control group were subjected to subcutaneous injection with loperamide at 3 mg·kg^-1 twice daily （total dose of 6 mg·kg^-1·d^-1） for 7 days to induce a model of FC. Drug administration was initiated on day 3 of modeling， which continued throughout the modeling period. The small intestinal propulsion rate of each group was measured via the ink propulsion method. LC-MS/MS was employed to measure the fecal bile acid content in each group， and the serum bile acid level was measured by a microplate. The 5-hydroxytryptamine （5-HT） and cyclic adenosine monophosphate （cAMP） levels in the colon tissue of each group were measured by enzyme-linked immunosorbent assay （ELISA）. Western blot was employed to determine the protein levels of apical sodium-dependent bile acid transporter （ASBT） in the ileum terminus and Takeda G protein-coupled receptor 5 （TGR5） in the colon. ResultsCompared with the normal control group， the model group exhibited decreases in the small intestinal propulsion rate and total fecal bile acid content （P<0.01）， an elevation in the serum total bile acid level， lowered 5-HT and cAMP levels （P<0.01）， up-regulation in the protein level of ASBT （P<0.01）， and down-regulation in the protein level of TGR5 （P<0.05）. Compared with the model group， the positive control， SEHW， and QL groups showed increases in the small intestinal propulsion rate （P<0.05， P<0.01） and total fecal bile acid content （P<0.01） and a decline in the serum bile acid level. Compared with the SEHW group， the SC group had decreased total fecal bile acid content （P<0.01） and an elevated serum bile acid level， while the QL group showed increased total fecal bile acid content （P<0.01） and a lowered serum bile acid level. Compared with the model group， the positive control， SEHW， and QL groups demonstrated down-regulation in the protein level of ASBT （P<0.05， P<0.01） and up-regulation in the protein level of TGR5 （P<0.05， P<0.01）. The SC group showed no significant change in the protein level of ASBT and up-regulation in the protein level of TGR5 compared with the model group （P<0.05）. Additionally， compared with the model group， the positive control， SEHW， and QL groups showed elevated 5-HT and cAMP levels （P<0.05， P<0.01）. The SC group showed no significant difference in the 5-HT level but a rise in the cAMP level （P<0.01）. ConclusionSEHW， through the compatibility of QL and SC， demonstrates a dual regulatory effect on bile acid metabolism， embodying the principle of descending turbidity and ascending lucidity， which highlights the compatibility and scientific rationale of this formula. SEHW inhibits ASBT expression in the ileum to reduce ileal bile acid reabsorption， increase colonic bile acid content， and bind to colonic TGR5 to release 5-HT， thereby enhancing intestinal motility and promoting intestinal contraction.

Objective:To understand the mediating role of resourcefulness between perceived stress and depression in primary caregivers of stroke patients.Methods:The primary caregivers of 311 inpatients with stroke in three Class Ⅲ Grade A hospitals in Yinchuan from December 2020 to August 2021 were selected by convenience sampling and investigated with the general information form, Resourcefulness Scale (RS) , Chinese Perceived Stress Scale (CPSS) and Self-Rating Depression Scale (SDS) . SPSS 25.0 and PROCESS were used to analyze the relationship between the three, and to explore the mediating role of resourcefulness between perceived stress and depression in the primary caregivers of stroke patients.Results:In this study, 311 questionnaires were distributed, 311 questionnaires were recovered, and 308 questionnaires were valid. The primary caregivers of stroke patients scored (24.98±6.38) for perceived stress, (83.98±12.85) for resourcefulness, and (44.35±8.40) for depression. Resourcefulness was negatively correlated with perceived stress and depression scores ( r=-0.313, -0.317; P <0.01) , and perceived stress was positively correlated with depression ( r=0.425, P<0.01) . The Bootstrap method further validated that resourcefulness played a partial mediating role between perceived stress and depression, and the mediating effect accounted for 14.95% of the total effect. Conclusions:Resourcefulness is a mediating variable between the perceived stress and depression of the primary caregivers of stroke patients, and resourcefulness plays a partial mediating role between perceived stress and depression.