Background and purpose:The genesis and progression of cervical cancer are closly related to E6 and E7 oncogenes of HPV.Special ribozyme and antisense oligonucleotide could inhibit the expression of E6 or E7.but both of them were not the best methods.The aim of this study was to investigate the inhibitive effect of chemically synthetic siRNA on E6 gene in the cervical cancer cell line SiHa and the following biological changes of SiHa cells.Methods:Three specific siRNAs targeting to HPV16 E6 were synthesized and transfected into SiHa cells by lipofectamine.The level of E6 mRNA was tested by RT-PCR,proliferation activities,the cell cycle distribution,expression of p53 protein and the ultramicrostructure changes of SiHa cells were detected by MTT,FCM,immunochemistry and TEM respectively.Results:All the 3 siRNAs could inhibit the level of E6 mRNA singificantly,among which siRNA1 was most effective.Decreased cellular proliferation activity was observed by MTT,especially when the cells were transfected with siRNA1 at 50 nmol/L concentration.Flow cytometry revealed obvious G1 arrest in cell cycle.The expression of p53 protein in transfected cells(0.75?0.06)was increased compared to the control group(0.43?0.03),the difference was singificant.Cell-substance concentration and vacuolus were found in endochylema by TEM.Conclusions:Chemically synthetic siRNA can interfere in the expression of E6 mRNA in SiHa cells specifically and induce the biological changes of the target cells.

【Objective】 To reduce the incidence of postoperative intestinal obstruction, we tried to improve surgical techniques by closing the cavity formed during radical cystectomy + ileal passage (Bricker) via laparoscopy to prevent the formation of abdominal hernia. 【Methods】 During Oct.2018 and Feb.2022, 41 patients were involved (conventional group). After standard laparoscopic radical cystectomy + pelvic lymphadenectomy, the ileum channel was established. The right medial retroperitoneum was sutured to cover the mesothelium and end of the ileum channel under open operation or endoscope. The space between the ureter and mesothelium of the ileum channel was sealed, and the end of the ileum channel and both ureters were externalized. During Feb.2022 and Dec.2022, 15 patients were involved (modified group). The right inner and outer lateral peritoneums below the ileal conduit were sutured to "bottom out" the gap between the ileal conduit and the right abdominal wall in addition to standard procedures. The recovery of intestinal function and incidence of bowel obstruction were compared between the two groups. 【Results】 In the conventional group, the intestinal function recovered within 2 to 6 days after surgery, with a median ventilation time of 3 days. Intestinal obstruction occurred in 3 patients, 2 of whom improved after conservative treatment while 1 underwent surgical exploration after ineffective conservative therapy. There were no significant differences in the time of discharge and ventilation between the two groups, but no intestinal obstruction occurred in the modified group. 【Conclusion】 Peritoneal externalization at the end of ileal passage can reduce the incidence of intra-abdominal hernia and postoperative intestinal obstruction, which is worthy of clinical application.