Objective: To study the mechanism of differentiation of rat ectomesenchymal cells to odontoblasts. Methods: Ectomesenchymal cells were cultured in three-dimension culture model using collagen gel as frame, and the change of phenotype of ectomesenchymal cells were observed and detected by phase-contrast microscopy and immunohistochemistry after the cells had been treated by 10 ng/ml of bFGF or/and 100 ng/ml IGF-1. Results: 4 days after treatment by bFGF and IGF-1, the cells appeared to be odontoblast-like cells aligned parallelly and polarized with long cytoplasmic processes attached to one end of the cell body.The cells were positive for DSP expression. However, the cells were DSP negative and aligned disorderly in other groups. Conclusion: Ectomesenchymal cells can be induced to differentiate to odontoblast-like cells in three-dimension culture model with the treatment by bFGF and IGF-1.

To evaluate therapeutic efficacy and safety of Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection in the treatment of chronic obstructive pulmonary diseases (COPD). All clinical trial databases were retrieved from PubMed, EMBase, ClinicalTrials, Cochrane Library, CNKI, CBM, VIP, and Wanfang. Data were searched from inception to February 2018, randomized controlled trials (RCTs) by Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection combined with chemotherapy (experimental group) compared with conventional therapy (control group) in the treatment of COPD were included. All included studies were critically appraised by two independent reviewers according to the cochrane systematic review method and using Revman 5.3 Software and State 12.0 for Meta-analysis. There were 16 RCTs were included in the evaluation and screening of selected articles with a total of 1 259 patients. The Meta-analysis showed that the total clinical efficacy of the patients in the experimental group after treatment was significantly better than that in the control group [OR = 4.67, 95% CI (3.03, 7.19), P < 0.000 01]; The improvement of forced expiratory volume in one second (FEV1) was significantly better than control group [SMD = 1.43, 95% CI (1.14, 1.72), P < 0.000 01]; Forced vital capacity (FVC) was significantly better than control group [SMD = 1.53, 95% CI (1.17, 1.90), P < 0.000 01]; FEV1/FVC was significantly better than control group [SMD = 1.12, 95% CI (0.90, 1.34), P < 0.000 01]; FEV1 in the percentage of the predicted value forced vital capacity (FVC) was significantly better than control group [SMD = 0.62, 95% CI (0.31, 0.93), P < 0.000 1] and the blood gas index of PaO2 was significantly higher than control group [MD= 9.7, 95% CI (7.92, 11.65), P < 0.000 01]; PaCO2 was significantly lower than control group [SMD =-1.51, 95% CI (-1.90, -1.12), P < 0.000 01]; SaO2 was significantly higher than control group [SMD = 0.94, 95% CI (0.48, 1.40), P < 0.000 1]. For the treatment of chronic obstructive pulmonary disease, Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection combined with chemotherapy can significantly improve the lung function of patients, but the conclusions of the study still need to be confirmed by more high-quality clinical trials.

ObjectiveTo investigate the effects of ARB or/and β-blocker in treatment of dilated cardiomyopathy(DCM).Methods95 cases with DCM were randomly divided into 3 groups.ARB group(A,32 cases) was treated with Irbesartan,150 mg/d without adverse drug reaction and then to 300 mg/d weekly; β-blocker group( B,30cases) was treated with Bisoprolol,1.25 mg/d without adverse drug reaction and then to maximal dose weekly; combination group( AB,33 cases) was treated with Irbesartan and Bisoprolol to maximal dose which patients were able to tolerate.The 3 groups were observed for a period of 12 months.ResultsComparing with pretreatment,the results of 6min walk,LVEDd,LVESd,LVEF were improved significantly( all P ＜0.05).There were no significant differences between A group and B group(P ＞0.05),but the differences between combination group and A group or B group were significant( P ＜0.05 ).ConclusionEffect of ARB combined with β-blocker was better than that of ARB or β-blocker alone in treatment of DCM and well tolerated.

Objective To explore motivation alteration influencing factors of internet addiction disorder (IAD) undergraduates.Methods The study sampled randomly 793 undergraduates from China University.According to CIAS-R and Young's internet addiction diagnostic criteria,a total of 37 internet addicts were selected.Internet addiction survey was used to study the motivation alteration influencing factors of IAD undergraduates.Results (1) The negative effect of internet using on academy,personal relationship etc.(damage from internet using) had a significant positive correlation with motivation alteration (r =0.672,0.699,0.658,P ＜ 0.01 ; r =0.653,0.673,0.628,P ＜ 0.01 ; r =0.604,0.681,0.703,P ＜ 0.01).(2) Benefit from psychological satisfactory level of internet using had no significant difference with motivation alteration.(3) Self-efficiency of behavioral control on using internet had a significant negative correlation with motivation alteration (r =-0.397,-0.370,P ＜0.05).Conclusion The motivation alteration of IAD undergraduates is influenced by the factors of damage from internet using,sense of achievement,self-efficiency of behavioral control on using internet and so on.

Objective To compare the difference in clinical prognosis of patients with low malignant obstructive jaundice treated by percutaneous biliary stent insertion across or above the duodenal papilla.Methods 56 patients with malignant biliary obstruction were reviewed retrospectively.Stents were placed above the duodenal papilla in 31 cases (group A) and across the duodenal papilla in 25 cases (group B).Total bilirubin reduction rate after 4-7 days of the procedure, biliary infection rate and stent occlusion rate were evaluated and compared between two groups.Results Mean survival periods were 180.3±142.5 days for group A and 178.6±137.7 days for group B (P=0.840).Total bilirubin level was decreased by 42.0±43.6% for group A and by 41.4±28.7% for group B after 4-7 days of the procedure(P=0.950);clinical success rates were 93.5% for group A and 92.0% for group B (P=1.0).Post-procedure cholangitis occurred in 7 cases (22.6%) in group A and 5 cases (20.0%) in group B (P=0.815).Stent occlusion rates were 22.6% and 28.0% for group A and group B (P=0.642).Conclusion For patients with lower malignant biliary obstruction, both of the two modalities of stent placement are safe and effective treatment.Stent placement across the duodenal papilla do not increase the development of stent occlusion or cholangitis compared with stent placement above the duodenal papilla.

Objective To study the chemical constituents from Calystegia sepium. Methods The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20, ODS column chromatography, preparative HPLC and so on. Their structures were determined on the basis of physicochemical properties and their spectroscopic data, as well as literatures. Results A total of 20 compounds were separated and identified as tyrosol (1), quercetin-3-O-β-D-glucopyranoside (2), scutellarein-7-O-β- glucopyranoside (3), kaempferol-3-O-β-D-glucopyranoside (4), kaempferol-3-O-galactoside (5), kaempferol-3-O-β-robinobioside (6), nicotiflorin (7), rutin (8), uridine (9), tachioside (10), 2-methoxy-4-(2’-hydroxyethyl)-phenyl-1-O-β-D-glucopyranoside (11), 4-hydroxyphenethylol-4-O-β-D-glucopyranoside (12), polybotrin (13), pyridine-3,5-dicarboamide (14), skimmin (15), benzylalcohol-β-D-glucopyranoside (16), 5-hydroxyl-2-hydroxymethylpyridine (17), picein (18), 5-hydroxymethyl-furaldehyde (19), and sessiline (20). Conclusion Compounds 1-20 are isolated from Calystegia sepium for the first time.

Objectives Aspidin BB, a typical phloroglucinol derivative from Dryopteris fragrans, possesses significant antifungal property. This study aimed to investigate potential mechanism of antifungal activity of Aspidin BB against Trichophyton rubrum which is the most common pathogens responsible for chronic dermatophytosis. Methods The minimum inhibitory concentration (MIC) of Aspidin BB against strains was determined by broth microdilution. The effects of Aspidin BB on ergosterol biosynthesis were investigated by content determination based on UPLC method. Besides, the effects of drugs on squalene epoxidase (SE) in T. rubrum cell membrane were analyzed. Results MIC value of Aspidin BB against T. rubrum was 25.0 μg/mL. Aspidin BB reduced ergosterol content significantly, but no notable effect on squalene epoxidase activity. Conclusion The results suggested that Aspidin BB inhibited ergosterol biosynthesis. However, it was not squalene epoxidase but other components may sever as possible targets in ergosterol biosynthesis pathway.

Objective: To establish the process technology for vinegar-steamed Cyperi Rhizoma and provide a reference for improving the quality of vinegar-steamed Cyperi Rhizoma. Methods: Using extract detection, and volatile oil content detection analysis method, the influences of materials rice vinegar dilution times, moistening time, steamed pressure, temperature, and time on the quality of vinegar-steamed Cyperi Rhizoma were investigated. Results: The appearance and index components of vinegar-steamed Cyperi Rhizoma obtained from different processing technologies were compared. The results showed that the best effect was obtained through taking the vinegar with 20% weight of medicine, diluting by the vinegar with 20% weight of water to homogenization, and moistening for 70 h under the condition of steaming pressure 0.10 MPa, temperature of 110°C, steaming for 4.5 h (big medicinal materials) or 4 h (small medicinal materials). Conclusion: The processing technology is stable and reliable. Compared with the processing technology in Chinese Pharmacopoeia 2010, the present technology could be better for the quality control of vinegar-steamed Cyperi Rhizoma and provide the scientific basis for processing vinegar-steamed Cyperi Rhizoma.

Adolescent ; Adult ; Arteriovenous Fistula ; surgery ; therapy ; Child ; Child, Preschool ; Female ; Heart Defects, Congenital ; surgery ; therapy ; Heparin ; therapeutic use ; Humans ; Infant ; Male ; Middle Aged ; Pulmonary Artery ; abnormalities ; surgery ; Pulmonary Veins ; abnormalities ; surgery ; Retrospective Studies ; Telangiectasia, Hereditary Hemorrhagic ; surgery ; therapy ; Young Adult

OBJECTIVETo investigate the mechanisms of Kebimin decoction (KD) in treating allergic rhinitis (AR).

METHODSSixty patients with AR were randomly divided into two groups, patients in the treated group (TG) were treated with KD, those in the control group (CG) were given Xinfang rhinitis capsule. The serum levels of adhesion molecule (sICAM-1, P-selectin), Th1 cytokines, including interferon-gamma (IFN-gamma) and interleukin-2 (IL-2), and Th2 cytokines (IL-4 and IL-5) were determined by enzyme-linked immunosorbent assay (ELISA) before and after treatment, and compared with those in 30 healthy subjects as control.

RESULTSThe serum levels of adhesion molecule and Th2 cytokines were higher and Th1 cytokines were lower in AR patients than those in healthy control (P < 0.01). After treatment, these indexes were restored significantly (P < 0.01) in the treated group, but no significant change was found in the control group.

CONCLUSIONKD has definite effect in treating allergic rhinitis by inhibiting production of adhesion molecule and regulating the levels of Th1/Th2 cytokines to correct the imbalanced Th1/Th2 cytokines network.

Adolescent ; Adult ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Intercellular Adhesion Molecule-1 ; blood ; Interferon-gamma ; blood ; Interleukin-2 ; blood ; Interleukin-4 ; blood ; Interleukin-5 ; blood ; Male ; Middle Aged ; P-Selectin ; blood ; Phytotherapy ; Rhinitis, Allergic, Perennial ; drug therapy ; metabolism ; Th1 Cells ; metabolism ; Th2 Cells ; metabolism