Aim To compare the bioavailability of two ubiquinone tablets in healthy volunteers. Methods A HPLC method was used to determine the serum ubiquinone 10 concentrations at 0,1,2,3,4,6,8 and 12 h after oral administration for 7 days ( 20 mg, tid ) in a cross-over test. Results AUC, Cmax and Tpeak of the test tablets were (5.91?1.78)?g?h?ml-1 ,(0.66?0.17)?g?ml-1 and (4.00?1.25) h, respectively,and these of the reference tablets were (6.30?2.09)?g?h?ml-1,(0.70?0.20)?g?ml-1 and (4.60?1.58)h , respectively . All of these parameters between the two kinds of tablets were not significantly different statistically. Conclusion The related bioavailability of the test tablets versus the reference tablets is 93.9%. The two formulations of ubiquinone 10 are bioequivalent.