AIM: To investingate the variety of the function of vascular endothelium in the patients with hypertensive nephropathy and the effect of Huayu Qinli Granule on (Hirudo,Rhizoma Smilacis Glabrae,Herba Leonuri,etc.) it. METHODS: This was a prospective,comparative and single-blind clinical trial.Patients in accord with the diagnosis and TCM differentiation standard were randomly divided into treatment group,united treatment group,blank control group and positive control group.Before the beginning of the trial,all patients were treated by Felodipine to lower their blood pressure to desired value.When the trial began,blank control group were treated only by Felodipine, positive control group were treated by general hypertension pills+captopril,treatment group were treated by Felodipine+Huayu Qinli Granule,united treatment group were treated by Felodipine+Huayu Qinli Granule+captopril.The course of study was for 4 weeks.The levels of urinary microalbumine(ALB),urinary ?_1-microglobulin(?_1-mg),urinary?_2-microglobulin(?_2-mg),serum nitric oxide and plasma endothelin were measured before and after the trial. RESULTS: The results showed both Huayu Qinli Granule and ACEI improved the levels of serum nitric oxide but there was slightly advantage over ACEI;both Huayu Qinli Granule and ACEI improved the levels of plasma endothelin,the improvement of Huayu Qinli Granule was(equal) to ACEI;united treatment group had the remarkable effect on serum nitric oxide and plasma endothelin. CONCLUSION: The results indicate that Huayu Qinli Granule improves the function of vascular endothelium in the patients with hypertensive nephropathy to a certain extent with the sufficiency of the treatment of depressing blood pressure.The effect of Huayu Qinli Granule is similar to that of ACEI.They cooperate with each other but mechanism of them may be different.

Objective To evaluate the academic level and the popularity of Chinese Journal of Orthopaedics from the point view of citation. Methods According to the information of Chinese Medical Citation Index (CMCI), the amount and distribution of the original articles in Chinese Journal of Orthopaedics cited by the journals included by CMCI were statistically analyzed. The data analyzed included the percentage of cited articles, the number of citation of a single article, authors of most cited articles, geological regions of cited articles, years of citation, citing journals and self citing rate. Results The percentage of cited papers (749 articles, 4213 times) in all published 1184 articles in Chinese Journal of Orthopaedics from 2000 to 2004 were 76.43%, 75.20%, 71.49%, 57.46% and 30.19%, with an average of 63.26%. The original articles cited by other researchers were 7.86, 6.54, 5.48, 2.98 and 1.78, with an average of 5.62. The highest one was citated by 68 times. The most frequently cited authors were from 30 provinces of China and the city Beijing, Shanghai and Tianjin were the three topest cities among other cities in the naion. There were 461 citing journals, and there was 191 items self citsted, self-citing rate is 0.045. Conclusion Chinese Journal of Orthopaedics has published high quality articles. It has been become one of the most important information resource for orthopaedic researchers and one of the most important medical journals in China.

Bibliometrics ; China ; Occupational Health ; statistics & numerical data ; Periodicals as Topic ; statistics & numerical data

AIM: To investigate the topography of the anterior surface of the human crystalline lens. ·METHODS: A Non-contact three-coodinate measuring system was utilized to scan the anterior surfaces of 8 human eye lenses in vitro. Acquired data were processed and computer models of the anterior lens surfaces were made using the program (Surfacer v 10.0). The to-pography of the anterior lens surfaces were established. Radii of curvature were measured at preset spots over the lens surfaces and two way analysis of variance was performed. The data were analysed to determine whether the radius of curvature varied systematically with the position on the lens surface from which the measurement was made. Lens surface asymmetric index (LSAI) was defined and calculated. The vertical and horizontal meridians of the modeled lens were calculated, and the best curve fit to any conic section was determined. ·RESULTS: The topography of the anterior lens surface indicated that the central zone (the central radius of curvature is (9.09±0.80)mm was steeper than that of the peripheral zone(17.05±2.20)mm. Two way analysis of variance of the radii of curvature at preset spots over the lens surfaces showed that the differences were statistically significant (P ＜ 0.05). Curve regression of radii of curvature at preset spots and their distances to the surface center revealed that the correlation of them was the third power function. LSAI increased steadily from the lens surface center(0.013±0.005) to the periphery(0.184± 0.065). The dots on the horizontal and vertical meridians were fit to four kinds of curves, and the determinate coefficient of hyperbola fit were the largest (0.9989-0.9999). ·CONCLUSION: The anterior lens surface is imperfectly rotational symmetric. Moreover, the nearer to the center, the more rotational symmetric it is. Radii of curvature increase nonlinearly from the surface center to the periphery. Anterior lens surface is typically hyperbolic.

Objective To analyze the core authors, and their organizations, geographical regional distribution of Chinese Journal of Orthopaedics. Methods The core authors, coauthors and their organizations whose articles were published in Chinese Journal of Orthopaedics between 1995 and 2004 were quantitatively analyzed using literature search through CMCI, which programmed by the People's Liberation Army Medical Library and their geographical regions distribution, the organizations of whom had high quantitative publication were determined using literature metrology methods. Results There were 2660 articles published in Chinese Journal of Orthopaedics between 1995 and 2004. All of 1123 authors of 2660 articles had only one article published which accounted for 69.67% of total first authors on the Chinese Journal of Orthopaedics in ten years. There were 2385 articles with one or more coauthors and the cooperative rate was 89.66%, and cooperative degree was 4.11 in the period of time. There were 275 articles with single author, which accounted for 10.34% of total articles. The articles written by the authors from military hospitals, university affiliated hospitals and provinces-level hospitals were 646(24.28%), 1091(41.01%) and 706(26.54%), respectively during the period. There were 218 core authors with 959(36.05%) articles published and there were 20 high-quantities organizations with 1007(37.86%) articles published in this study. The number one region of published articles was Beijing with 667(25.08%) articles. There were 2443 (91.84%) articles pressed from the organizations of affiliated hospitals of universities, provinces-level hospitals and army hospitals. Among them, Tianjin hospital (101 articles), Beijing Jishuitan hospital (100 articles) and affiliated Xijing hospital of the 4th military medical mniversity (79 articles) occupied 10.53% of the pressed papers and they were in the first three positions. Conclusion Authors of Chinese Journal of Orthopaedics have a wide distribution and highly cooperative rate. There were a group of active and talented core authors who has a great influence on the journal.

Articulation Disorders ; surgery

AIM:To observe and compare clinical effects of coaxial 1. 8mm microincision phacoemulsification and 3. 2mm small incision phacoemulsification.
●METHODS:A total of 117 eyes of 85 patients with age-related cataract in our hospital were divided randomly into two groups:43 patients (59 eyes) in the coaxial 1. 8 mm microincision cataract surgery group ( C - MlCS ) , 42 patients (58 eyes) in the coaxial 3. 2 mm traditional small incision cataract surgery group (C-SlCS). A total of 117 eyes were received phacoemulsification with intraocular lens implantation. Uncorrected visual acuity was recorded preoperatively and postoperatively at 1, 7, 30 and 90d. The effective phacoemulsification time and average ultrasound energy were recorded in surgery. Corneal endothelial cell and corneal topography were recorded preoperatively and postoperatively at 90 d.
●RESULTS:Uncorrected visual acuity ( logMAR) was no overall statistical significance difference between C-MlCS group and C-SlCS group (P>0. 05), but was significant statistical difference in different time-point within both groups(P<0. 05). Uncorrected visual acuity in different time-point had nothing to do with corneal wound size in cataract surgery(P>0. 05). On the 1 day after surgery, uncorrected visual acuity was 0. 16±0. 11 in C-MlCS group and 0. 22±0. 18 in C-SlCS group(P<0. 05). AVE was (7. 00± 2.72)% in C-MlCS group and (6. 16±3. 16)% in C-SlCS group (P>0. 05). EPT was (3. 09±1. 61)s in C-MlCS group and (3. 20±1. 92)s in C-SlCS group (P>0. 05). At 90 d after surgery, corneal endothelial cell loss percentage was (5. 81±2. 28)% in C-MlCS group and (5. 69±2. 38)% in C-SlCS group (P>0.05), SlA was (0.35±0.11) Din C-MlCS group and (0. 61±0. 13) D in C-SlCS group (P<0. 05).
● CONCLUSION: Compared with coaxial 3. 2mm traditional small incision cataract surgery, 1. 8mm coaxial microincision cataract surgery can get earlier visual rehabilitation and significantly reduce SlA. The coaxial 1. 8mm microincision cataract surgery is safe, effective and deserves further clinical applications.

AIM:To investigate the efficacy of controlled continuous curvilinear capsulorhexis(CCC) technique in short axial length and shallow anterior chamber eyes.METHODS:Sixty-eight patients(68 eyes) with short axial length and shallow anterior chamber were included.The routine CCC technique was used in 32 cases (32 eyes) and controlled CCC technique was used in 36 cases (36 eyes).The success rate and complication were compared between two groups. RESULTS:The success rate of the routine technique group and controlled technique group was 53. 13% and 86.11% respectively. Incomplete CCC leading to posterior capsule tears was 9.38% and zero in two groups respectively.CONCLUSION: Controlled CCC technique can increase the success rate and reduce complications in short axial length and shallow anterior chamber eyes.KEYWORDS:phacoemulsification; continuous curvilinear capsulorhexis; complication

Background Vitronectin is a glycoprotein that has a variety of functions.Its expression was markedly higher in the retina of oxygen induced mice,which was confirmed in our animal model,and also increased in human umbilical vein endothelial cells (人 UVECs) that were cultured in high glucose.However,there was no evidence that showed vitronectin was involved in retinal neovascularization.Objective This study was to observe the influence of vitronectin on cytoskeleton remodeling,cell migration and blood vessel formation in 人 UVECs conditioned by high glucose.Methods 人 UVECs were cultured in high glucose and the expression of vitronectin was knocked down using RNA interference technology.The experiments were divided into the high glucose group (人 UVECs were conditioned with DMEM medium that contained 50 mmol/L glucose),negative interference group (人 UVECs were transfected with control siRNA in advance,and then were conditioned with DMEM medium that contained 50 mmol/L glucose) and positive interference group (HUVEC were transfected with vitronectin siRNA in advance,and then were conditioned with DMEM medium that contained 50 mmol/L glucose).The protein expression of vitronectin was measured by Western blot,and the microfilament cytoskeleton of 人 UVECs was examined by immunofluorescence cytochemical staining followed by fluorescence microscopy.Cell migration ability in a scratch wound assay and blood vessel formation ability in a matrigel assay of 人 UVECs were evaluated.The general differences were analysed by One-Way ANOVA ;further contrasts of the two groups were analysed by the LSD-t test.Results The differences in vitronectin expression of the three groups were not obvious at 0 hour (F=1.064,P＞0.05).After 24 hours,vitronectin expression was highest in the high glucose group,lower in the negative interference group,and the lowest in the positive interference group,and the differences were significant (F =15.519,P＜0.05).After 48 hours,vitronectin expression of the three groups displayed the same pattern,and the differences were also significant (F=37.521,P＜0.05).Immunofluorescence showed that the cytoskeleton structure was most obvious in the high glucose group,moderate in the negative interference group,and was the least obvious in the positive interference group,after both 24 hours and 48 hours.In the scratch wound assay,the cell migration ability of the high glucose group was the highest,lower in the negative interference group,and the lowest in the positive interference group after 24 hours,and the differences were significant (F=90.685,P＜0.05).After 48 hours,the cell migration abilities of the three groups displayed the same pattern,and the differences were also significant (F=67.880,P＜0.05).In the matrigel assay,after 6 hours,the number of blood vessels formed in the high glucose group was more than that in the negative interference group,and the least amount was found in the positive interference group.The differences among of them were significant (F =86.653,P＜0.05).The number of blood vessel formed in the positive interference group was also the lowest after 12 hours,and the differences were also significant (F=18.992,P＜0.05).Conclusions Vitronectin can bring about cytoskeleton remodeling,increase in cell migration,and enhancement of blood vessel formation in 人 UVECs conditioned in high glucose.It may be one of the important influence factors of diabetic retinopathy.