1.Effects of curcumin on apoptosis and caspase-3 expression in cortex tissue in rats with hypoxic ischemic brain damage.
Guang-Mei YIN ; Lin-Sheng YU ; Shu-Zhen WU ; Guang-Hua YE
Chinese Journal of Applied Physiology 2010;26(4):504-506
Animals
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Apoptosis
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drug effects
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Brain
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drug effects
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Caspase 3
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metabolism
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Curcumin
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pharmacology
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Hypoxia-Ischemia, Brain
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metabolism
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pathology
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Male
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Rats
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Rats, Sprague-Dawley
2.STUDY ON THE FERMENTATION CONDITION PRODUCING 2-KETO-LGLUCONIC ACID BY USING MIXED CULTURE OF MICROORGANISM
Wen-Chu LIN ; Qing YE ; Chun-Hong QIAO ; Guang-Lin YIN ;
Microbiology 1992;0(04):-
Vitamin C precusor-2-keto-L-gulonic acid can be prepared directly by mixed culture of Ghiconobacter oxy-dans SCB329 and Guconobacter subaxydans SCB110. To obtained its high yield, firstly, the proportion of the two micro- organisms, the ingredients of medium and the initial pH were optimized in shake flaskd, then L9 (34) orthogonal experiment confirmed that urea, C. S. L had high degree statistical meaning. Based on these data, an optimized fermentation media was obta ined: D-Sorbitol 9g, C. S.L1.5g, Urea1.5g, KH2PO40.1g, CaCO30.2g. By-product can be inhabited to the greatest extent and the yield increases by 20%.
3.A new bisabolane-type sesquiterpenoid from Coreopsis tinctoria.
Jin-jun LIU ; Yin-jun YANG ; Yin-di ZHU ; Guang-zhi LI ; Wen-hua HUANG ; Bao-lin GUO
China Journal of Chinese Materia Medica 2015;40(11):2132-2137
To study the chemical constituents of the inflorescences of Coreopsis tinctoria from Xinjiang, isolation and purification of constituents were carried out by column chromatography on macroporous resin (D101) , MCI gel, MDS gel, silica gel, Sephadex LH-20 and semi-preparative HPLC. The structures of the compounds were identified by physicchemical properties and spectral data analysis. Fourteen compounds were isolated and identified as coretinterpenoid A (1), coretinphenol (2), quercetin (3), quercetin-3-O-β-glucopyranoside (4), luteolin (5), taxifolin (6), 7, 3', 5'-trihydroxyflavanone (7), isookanin (8), isookanin-7-O-β-D-glucopyranoside (9), 5, 7, 3', 5'-tetrahydroxyflavanone-7-O-β-D-glucopyranoside (10), butein (11), okanin (12), sulfuretin (13), and linocinnamarin (14). Compound 1 was a new isabolane-type sesquiterpenoid and compounds 4, 10 and 13 were isolated from this plant for the first time.
Coreopsis
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chemistry
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Sesquiterpenes
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chemistry
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isolation & purification
4.The Microbe Cell-surface Engineering Study Progress
Jing SHI ; Yong-Guang YIN ; Guei-Lin ZHANG ; Jian-Qun YU ;
Microbiology 1992;0(05):-
Microbe cell-surface engineering , which use the microbe cell surface display technology to display foreign proteins on the microbe cell surface to produce cell-surface proteins, was developed in recent years. I t can be utilizedto develop cell-catalyst, cell-adsorbent , live vaccine, biosensor and so on, and have a wide application perspective. But in our county, the microbe cell-surface engineering is studied just now. This review explain the development of the microbe cell surface engineering, overview the study and progress of microbe cell-surface engineering, and look this technology into the future.
5.Antidepression Action of Curcumin and Its Mechanism
Wenxing CHEN ; Leping LIU ; Lin LI ; Yin LU ; Guang CHENG ; Ning LI
Traditional Chinese Drug Research & Clinical Pharmacology 2000;0(05):-
Objective To study the antidepression action of curcumin and to explore its mechanism. Methods Mouse reserpine-induced depression model and mouse tetrabenazine-induced acquired despair model were used to observe the effect of curcumin on relieving depression. According to the hypothesis of monoamine, the effect of curcumin on activating monoamine, and inhibiting reuptake of monoamine neurotransmitters and monoamine oxidase inhibitor (MAOI) were observed. Results Curcumin in the dose of 50 mg/kg and more can relieve melancholic symptoms in mice induced by reserpine and tetrabenazine. Curcumin had no direct activation on monoamine either had no obvious inhibition on reuptake of monoamine neurotransmitters of noradrenalin, 5-hydroxytriptamine and dopamine . However, Curcumin had an obvious effect on improving rat forelimb spasm induced by tryptamine hydrochloride. Conclusion Curcumin acts like a kind of monoamine oxidase inhibitor, which exerts antidepression action by inhibiting monoamine oxidase activity and increasing the concentration of monoamine neurotransmitter in brain.
6.Synthesis and biological evaluation of 2-(3-butynoicamidophenyl) benzothiazole derivatives as antitumor agents.
Gui-Lin YIN ; Yan LI ; Ke TANG ; Xiao-Feng JIN ; Xiao-Guang CHEN ; Li LI ; Zhi-Qiang FENG
Acta Pharmaceutica Sinica 2014;49(6):888-895
A series of 2-(3-butynoicamidophenyl)benzothiazole derivatives were synthesized starting from 4-fluoro-3-nitrobenzoic acid. Structures of all the synthesized compounds were confirmed by 1H NMR and HR-MS. Their antitumor activities against human tumor cells lines (HCT116, Mia-PaCa2, U87-MG, A549, NCI-H1975) were evaluated by MTT assay. The results revealed that most of the synthesized compounds showed potent activities against HCT116, Mia-PaCa2, U87-MG tumor cells lines. Particularly, compounds 14c and 14h exhibited better activity with IC50 values of 1 x 10(-8) mol x L(-1) against U87-MG and HCT116 respectively. The structure-activity relationship of compounds was also discussed preliminarily.
Antineoplastic Agents
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chemical synthesis
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pharmacology
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Benzothiazoles
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chemical synthesis
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pharmacology
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Cell Line, Tumor
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Drug Screening Assays, Antitumor
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Humans
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Nitrobenzoates
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Structure-Activity Relationship
7.Relationship between the polymorphism of lipoprotein lipase gene and serum lipid levels in the Guangxi Hei Yi Zhuang population
Yong WANG ; Rui-Xing YIN ; Guang-Qin CHEN ; Wei-Xiong LIN ; De-Zhai YANG ; Shang-Ling PAN ;
Chinese Journal of Laboratory Medicine 2003;0(09):-
0.05 for all).Conclusions The allelic frequencies of LPL gene at Pvu Ⅱ locus in Hei Yi Zhuang were different from those in Han,but the genotypie frequencies in Hei Yi Zhuang were not different from those in Han.There was no significant correlation between the polymorphism of LPL gene at Pvu Ⅱ site and the serum lipid levels in two ethnic groups.
8.Correlation between level of TSH receptor antibody and activity of Graves ophthalmopathy after treatment with glucocorticoid
Hai-Bing JU ; Guang-Lin WANG ; Deng-Ming HU ; Zi-Zhen SHU ; Zi-Yin ZHU ; Fei-Fei SHEN ;
Chinese Journal of Endocrinology and Metabolism 2001;0(05):-
Fifty-six patients with Graves' ophthalmopathy(GO)were treated with antithyroid drug and oral prednisone for three months,TSH receptor antibody(TRAb)level was reduced,GO activity and severity of some patients were ameliorated but still positively associated with TRAb.It suggests that TRAb not only triggers off GO but also plays a possible role in the maintenance of the autoimmune process in GO.
10.Location and negotiation of second mesiobuccal canals in maxillary molars.
Cheng-fei ZHANG ; Rui-yu DING ; Xing-zhe YIN ; Bao-hong ZHAO ; Qiong-guang LIN
Chinese Journal of Stomatology 2003;38(2):86-88
OBJECTIVETo investigate prevalence, location, negotiation and the effect of operating microscope (OM) in the treatment of the second mesiobuccal canal (MB2) in maxillary molars.
METHODS113 maxillary molars were studied. The crowns of the teeth were removed 1 mm above the chamber bottom for searching MB2 canal. Initially location and negotiation of MB2 canal were attempted without OM. Teeth in which MB2 canal were not located or negotiated were further explored under OM. Canal orifices including MB orifice, MB2 orifice and palatal orifice were filled by red gutta-percha cones, then the teeth were scanned by scanner. The images were analyzed by Image-Proplus 4.0 software to measure the relationship between MB2 canal and other canals.
RESULTSThe MB-MB2 distance is (1.47 +/- 0.54) mm, MB-P distance is (5.77 +/- 0.66) mm, the vertical distance from MB2 to MB-P line is (0.53 +/- 0.28) mm, the angle between MB-P line and MB-MB2 line is 23.07 degrees +/- 13.08 degrees. MB2 orifices were located in 70 teeth (61.9%) and negotiated in 53 teeth (46.9%) without OM. With OM, additional MB2 orifices were located in other 6 teeth, 4 were negotiated; and 2 were negotiated in which MB2 canal were not negotiated without OM.
CONCLUSIONSMB2 canal can be located in 67.3% and negotiated in 52.2% of maxillary molars. Ability to locate and negotiate MB2 canal is facilitated by OM. The MB2 canal was located less than 1 mm mesially to the MB-P line and 2 mm palatally from the MB orifice.
Dental Pulp Cavity ; anatomy & histology ; Humans ; Maxilla ; Microscopy ; Molar ; anatomy & histology