Objective To explore the safety and feasibility of VOLUVEN and HES used for acute hyperv-olemic hemodilution on selective Hepatectomy .Methods 42 patients ( ASAⅠ ~Ⅱ) were randomized to the two groups(Ⅰ,VOLUVEN group;Ⅱ,HES group),20 minutes before the operation,the two groups of patients underwent AHH with VOLUVEN and HES respectively at the same quantity and speed ,and then the changes of haemodynamics , blood gas analysis ,electrolyte and coagulation indexes were detected .Results InⅠgroup,the changes of HB、HCT, PLT,central venous pressure had statistical significance before and after AHH (t=7.880,32.257,7.303,22.812,all P=0.000).InⅡgroup,the changes of HB,HCT,PLT,central venous pressure had statistical significance before and after AHH(t=5.398,14.924,11.171,5.620,all P=0.000).For inter-group,VOLUVEN has less effect than HES on central venous pressure(t=2.367,P=0.023).Conclusion Using VOLUVEN or HES can keep hemodynamics stable and improve the ischemic tolerance for AHH in liver surgery .VOLUVEN has less effect on central venous pressure.

Objective To manufacture the skin suction blister apparatus and observe the curative effect of autologous epidermal grafting in treating vitiligo.Methods According to the theory of local skin suction blister: local skin suction blister is decided by the two facts of the negative pressure and temperature,the skin suction blister apparatus was manufactured based on feedback principle,and was applied to autologous epidermal grafting for treating vitiligo.Results The performance of skin suction blister apparatus was stable,the operation was relative simple,and the total survival grade of autologous epidermal grafting was 98.01%.Conclusion The skin suction blister apparatus is simple to operate and its curative effect is excellent for clinical application.

Objective To design and manufacture the injecting roll booster,by which Dipospan mix liquid is injected for treating keloid.Methods According to the principle that screw roll can save labour when it is circumvolved forward,the injecting roll booster was developed.Dipospan mix liquid(Dipospan1ml,Hyaluronidase1500U,5-Fluorouracil 2~5ml) was injected for treating keloid.Results The treatment effect of the injecting roll booster was apparently better than that of general injector.The Dipospan mix liquid and Dipospan for keloid were injected,and the total efficacies were 94.45% and 85.51% respectively.There was marked difference statistically(p

With the development of hospital information construction,the focus has gradually changed from functions of the information system to data utilization.The utilization of medical data has great significance in health management,disease analysis,summary of treatment experience,and development of new technologies.By data mining technology,the paper extracts data related to the author's hospital,starts from intelligent diagnosis and simulates the establishment of the knowledge base for hospital clinical diagnosis.Upon verification,the relevance judged by the system is basically consistent with the clinical practice.Thus,it proposes the possibility to extend the application to hospital clinical pathways.

Acute Disease ; Humans ; Paraquat ; poisoning

Drugs, Chinese Herbal ; chemistry ; Humans ; Isoflavones ; isolation & purification ; pharmacology ; Phytoestrogens ; Plant Preparations ; isolation & purification ; pharmacology ; Plants, Medicinal ; chemistry ; Platelet Aggregation Inhibitors ; isolation & purification ; pharmacology ; Pueraria ; chemistry ; Scutellaria baicalensis ; chemistry

Objective:To analyze chlorogenic acid of Chaenomeles speciosa by high performance liquid chromatography-mass spectrometry. Methods:A method for determining chlorogenic acid of Chaenomeles speciosa by HPLC-MS is described. The operating conditions were Diamonsil C18 column (5μm, 4.6×250 mm) at room temperature, acetonitrile-0.05%phosphoric acid solution as mobile phase by step gradients elution at a flow of 0.8 ml/min and UV detection at 325 nm. Results: The content of chlorogenic acid of Chaenomeles speciosa is 1.3 mg/g (RSD 0.4%), respectively. Conclusion:Chlorogenic acid was detected from Chaenomeles speciosa, which may be related to its inhibition of FAS and the potential role of anticancer.

Objective:To detect the anti-cancer component, trans-resveratrol in red wine by high performance liquid chromatography, and to determine the influence of environmental factors on its content. Methods: A method for determining trans-resveratrol in red wine by HPLC is described. The operating conditions were ZORBAX-C18 column(4.6 mm×250 mm, 5μm) at room temperature, 30%acetonitrile as mobile phase at a flow of 1ml/min and UV detection at 306 nm. Results:The content of trans-resveratrol in Changyu dry red wine and Great Wall Claret Cabernet are (1.01±0.03)mg/L and (2.36±0.02)mg/L, respectively. The results show that, sunlight, high temperature and poor seal condition can decrease the content of trans-resveratrol. Conclusion:The content of trans-resveratrol varies in different brands of red wine. Red wine should be stored in cool, dark and sealed conditions.

Objective To evaluate the effect of heat treatment on ultraviolet B (UVB)-induced oxidative injury to human melanocytes.Methods Melanocytes were isolated from adult foreskins,and subjected to a primary culture.After 3-4 passages of subculture,the melanocytes were classified into 4 groups:control group incubated at 37 ℃,heat treatment group incubated at 42 ℃ for 1 hour,UVB group exposed to UVB irradiation at 100 mJ/cm2,combination group receiving heat treatment at 42 ℃ for 1 hour followed by UVB irradiation at 100 mJ/cm2.After three successive days of treatment,MTT assay was performed to evaluate cell viability,a biochemical method to determine the activity of superoxide dismutase (SOD) and concentration of malonaldehyde (MDA),and flow cytometry to detect intracellular reactive oxygen species (ROS) and apoptosis in melanocytes.Results The cell survival rate,apoptosis rate,SOD activity,MDA concentration and ROS level were (100 ± 6.14)％,(4.66 ± 0.58)％,(53.39 ± 8.23) U/gprot,(1.09 ± 0.32) mmol/gprot,and 1070.85 ± 42.07 in the control group respectively.UVB exposure induced a significant increase in apoptosis rate (24.14％ ± 2.90％,P ＜ 0.001),MDA concentration (1.65 ± 0.33 mmol/gprot,P ＜ 0.01) and ROS level (1416.45 ± 79.12,P＜ 0.01),but a significant decrease in cell survival rate (50.23％ ± 5.36％,P＜ 0.01)and SOD activity (31.98 ± 1 1.89 U/gprot,P ＜ 0.01) in the UVB group compared with the control group,while the heat pretreatment markedly downregulated the UVB-induced increase in apoptosis rate (14.9％ ± 1.49％,P ＜ 0.001),MDA concentration (1.10 ± 0.26 mmol/gprot) and ROS level (1033.30 ± 68.41,P＜ 0.01),as well as the decrease in cell survival rate (74.12％ ± 6.17％,P＜ 0.01) and SOD activity (51.63 ± 6.55 U/gprot,P＜ 0.01) in the combination group.Conclusion Heat treatment could protect melanocytes from UVB-induced oxidative injury.

BACKGROUND:In recent years, calcitonin has been reported to have better clinical efficacy in the treatment of osteoarthritis, but its mechanism of action for osteoarthritis is rarely reported. OBJECTIVE:To investigate the effect of calcitonin on the cartilage morphology and proteoglycan expression in rats with osteoarthritis. METHODS:Thirty Sprague Dawley rats were randomly divided into three groups: model group, treatment group, and sham group. Anterior cruciate ligament transaction of the right limbs was implemented in the model and treatment groups, and only the joint cavity was exposed in the sham group. At 2 days after modeling, the treatment group received a daily subcutaneous injection of salmon calcitonin, 15 IU/(kg?d), and the model group and sham group were administered with normal saline at the same dose. The injection lasted for 6 weeks. At 10 weeks after modeling, the articular surface of the tibia of rats in each group was generaly observed; bone mineral density of the distal femoral bone and subchondral bone of the lateral and medial ankle were detected using X-ray test; bone morphology and proteoglycan secretion were measured by hematoxylin-eosin staining and toluidine blue staining, respectively. RESULTS AND CONCLUSION:The tibial articular surface was smooth and glossy in the sham group, but oxblood in the model group with large-area ulcers; the treatment group showed rough and local ulceration. Compared with the sham group, the bone mineral density of the subchondral bone of medial and lateral ankle were increased significantly in the model group (P < 0.05), but trabecular separation and proteoglycan content were decreased (P < 0.05). Compared with the model group, the bone mineral density of the subchondral bone of medial and lateral ankle were decreased significantly in the treatment group (P < 0.05), while the trabecular separation and proteoglycan content were increased (P < 0.05). These findings indicate that calcitonin has better protection on the cartilage of rats with osteoarthritis, and can promote bone the secretion of proteoglycan.