Objective To manufacture the skin suction blister apparatus and observe the curative effect of autologous epidermal grafting in treating vitiligo.Methods According to the theory of local skin suction blister: local skin suction blister is decided by the two facts of the negative pressure and temperature,the skin suction blister apparatus was manufactured based on feedback principle,and was applied to autologous epidermal grafting for treating vitiligo.Results The performance of skin suction blister apparatus was stable,the operation was relative simple,and the total survival grade of autologous epidermal grafting was 98.01%.Conclusion The skin suction blister apparatus is simple to operate and its curative effect is excellent for clinical application.

Objective To design and manufacture the injecting roll booster,by which Dipospan mix liquid is injected for treating keloid.Methods According to the principle that screw roll can save labour when it is circumvolved forward,the injecting roll booster was developed.Dipospan mix liquid(Dipospan1ml,Hyaluronidase1500U,5-Fluorouracil 2~5ml) was injected for treating keloid.Results The treatment effect of the injecting roll booster was apparently better than that of general injector.The Dipospan mix liquid and Dipospan for keloid were injected,and the total efficacies were 94.45% and 85.51% respectively.There was marked difference statistically(p

Objective To explore the safety and feasibility of VOLUVEN and HES used for acute hyperv-olemic hemodilution on selective Hepatectomy .Methods 42 patients ( ASAⅠ ~Ⅱ) were randomized to the two groups(Ⅰ,VOLUVEN group;Ⅱ,HES group),20 minutes before the operation,the two groups of patients underwent AHH with VOLUVEN and HES respectively at the same quantity and speed ,and then the changes of haemodynamics , blood gas analysis ,electrolyte and coagulation indexes were detected .Results InⅠgroup,the changes of HB、HCT, PLT,central venous pressure had statistical significance before and after AHH (t=7.880,32.257,7.303,22.812,all P=0.000).InⅡgroup,the changes of HB,HCT,PLT,central venous pressure had statistical significance before and after AHH(t=5.398,14.924,11.171,5.620,all P=0.000).For inter-group,VOLUVEN has less effect than HES on central venous pressure(t=2.367,P=0.023).Conclusion Using VOLUVEN or HES can keep hemodynamics stable and improve the ischemic tolerance for AHH in liver surgery .VOLUVEN has less effect on central venous pressure.

With the development of hospital information construction,the focus has gradually changed from functions of the information system to data utilization.The utilization of medical data has great significance in health management,disease analysis,summary of treatment experience,and development of new technologies.By data mining technology,the paper extracts data related to the author's hospital,starts from intelligent diagnosis and simulates the establishment of the knowledge base for hospital clinical diagnosis.Upon verification,the relevance judged by the system is basically consistent with the clinical practice.Thus,it proposes the possibility to extend the application to hospital clinical pathways.

Acute Disease ; Humans ; Paraquat ; poisoning

Drugs, Chinese Herbal ; chemistry ; Humans ; Isoflavones ; isolation & purification ; pharmacology ; Phytoestrogens ; Plant Preparations ; isolation & purification ; pharmacology ; Plants, Medicinal ; chemistry ; Platelet Aggregation Inhibitors ; isolation & purification ; pharmacology ; Pueraria ; chemistry ; Scutellaria baicalensis ; chemistry

Objective To observe the effects and its efficacy of Ozagrel on CD62p and CD63 expressions in platelets of patients with acute cerebral infarction(ACI).Methods 64 patients with ACI were devided into two groups:Ozagrel group and Xuesaitong group(control group).The expression levels of CD62p and CD63 in 64 patients with ACI(before and after treating)and that in normal people(normal group)were measured with whole blood flow cytometry.The clinical effect of treatment in the Ozagrel group and the control group were observed and compared.Results The expression levels of CD62p and CD63 in patients with ACI were higher than those in the normal group(all P

Objective To measure Tei index in patients with idiopathic or ischemia dilated cardiomyopathy, and to evaluate its correlation with other conventional indexes of echocardiography of left ventricular(LV) function,and its applicability in assessing cardiopulmonary exercise capacity in those patients.Methods Seventy consecutive patients (53?14 years) with heart failure(New York Heart Association [NYHA] class, Ⅱ to IV) who had received echocardiographic diagnosis, and believed to be suffering from dilated or ischemia cardiomyopathy were studied. Echocardiography were performed; LV volume as well as ejection fraction were measured from the apical view of the two-dimensional echocardiogram using a modified Simpson’s rule algorithm in all patients. The following variables: peak early transmitral filling velocity (E), late transmitral filling velocity (A), their ratio (E/A), the deceleration time of E (DT), and Tei index were measured by spectral Doppler echocardiography. Furthermore, all patients underwent a cardiopulmonary exercise using 6min walk test. Results Advanced NYHA class was associated with higher Tei index values. A positive correlation was found between Tei index and early /late filling velocity ratio, while a negative correlation was found between Tei index and A wave, E wave, DT, and LV EF. Tei index showed no correlation with heart rate, blood pressure or age. Compared with the parameters of left ventricular function Tei index had a closer relation to the distance of 6min walk. Stepwise regression analysis revealed that the Tei index and the late LV filling velocity were the only independent predictors for cardiopulmonary exercise capacity.Conclusion Tei index correlates inversely with LV performance and reflects disease severity, which is a useful complimentary variable in the assessment of cardiopulmonary exercise performance in patients with heart failure.

Objective To establish a serum protein fingerprinting technique with a pattern matching algorithm to distinguish Dukes A stage from colorectal cancer of Dukes B,C,D stage. Methods Serum samples were collected from both reserved group and test group, each of the groups comprised 10 patients of colorectal cancer in Dukes A stage and 68 patients in Dukes B,C,D stage. The sera of the reserved group was combined with the surface of the IMAC3 proteinchip. Then the data of SELDI TOF MS was read and analyzed by BioMarker Wizard software and BioMarker Pattern software to get a classificatory pedigree tree, which is a standard configuration that can distinguish the sera of colorectal cancer patients of Dukes A from colorectal cancer patients of Dukes B,C,D, and the standard was confirmed by double blind test in the test group. Results At the M/Z values of 8 320 Da, 8 604Da, 8 867Da and 15 872Da, the protein contents in reserved group are obviously different between the two classes of patients. The accuracy of classification was 97 4%(76/78), corresponding sensitivity was 100%(10/10) and corresponding specificity was 97 1%(66/68). By double blind examination in test group, the corresponding accuracy was 100%(10/10), the corresponding sensitivity was 94 1%(64/68) and the corresponding specificity was 94 1%(64/68). Conclusion Colorectal cancer of Dukes A can be quickly and exactly diagnosed by this method with high sensitivity and specificity. That will be widely used in clinical application

Objective To discuss the effect of interferon ?-2a on the radiosensitivity and cell cycle for nasopharyngeal carcinoma cell line CNE-I. Methods Interferon ?-2a was given by different concentration. Then the cells were radiated with X-ray (6?MV) and the cell survival rate was calculated. The change of cell cycle dynamics was measured with flow cytometry. Results The cell survival fraction was 0.62, 0.43 and 0.20 respectively after the interferon ?-2a was given by different concentration(0.5?106, 1.0?106 and 1.5?106?IU/L). The radiosensitization ratio was 1.16, 1.57 and 1.93 respectively. Compared with the control group, increasing cell percentage in G_1 and G_2+M phage and decreasing cell percentage in S phage were observed on 24 h after the interferon ?-2a(1?106?IU/L) was given(P