Acute Disease ; Humans ; Paraquat ; poisoning

OBJECTIVE: To establish a near infrared spectroscopy method for the determination of the concentration of tiopronin for injection. METHODS: By using optical fiber probes and 1 mm sampling accessories, the near infrared transmission reflectance spectra of tiopronin for injection from seven pharmaceutical factories were obtained. The partial least square method was applied to establish the model with HPLC as the reference method. RESULTS: The model had a good prediction performance. The value of the correlation coefficient (r2) was 99.0%, the mean difference of prediction for test sets was 1.30%, and the root mean square error of prediction (RMSEP) was 0.982%. CONCLUSION: NIR is a rapid, reliable and powerful method for the quantitation of tiopronin for injection, and can be applied to rapid drug determination on the spot and stability study. Copyright 2012 by the Chinese Pharmaceutical Association.

Objective To explore the pattern and quantify the heat shock protein HSP)70 and HSP70 mRNA in Vero-E6 cells after infection with Hantann virus(HTNV).Methods The expres- sion of HSP70 and change of its mRNA level were detected by immunocytochemical staining,nucleic acid hybridization in situ and RT-PCR.Results In situ hybridization and RT-PCR were used to eval- uate the level of HSP70 mRNA during Hantaan 76-118 infection.HSP70 mRNA increased 0.5 h after infection,reached its peak by 12 h and gradually declined to steady state level by 72 h(vs.sham infec- ted group,P＜0.05).The expression of HSP70 protein induced by Hantaan 76-118 infection was e- valuated by quantitative immunocytochemical staining.HSP70 increased 0.5 h after infection,reached its peak by 12 h and decreased at 72 h after infection(vs.sham infected group,P＜0.05).Conclu- sions HSP70 can be induced directly by HTNV infection at both mRNA and protein levels,It pro- vides a basis for the further study of the pathogenesis,prevention and treatment of hemorrhagic fever with renal syndrome(HFRS).

Objective To explore the effect of Qingretonglin pill combined with levofloxacin tablet on urinary tract infection after spinal cord injury. Methods 55 spinal cord injury patients with urinary tract infection were divided into control group (n=27) and observation group (n=28). Levofloxacin tablets were orally administered in both groups, 500 mg everyday, 14 days. The Qingretonglin pill was also orally administered in the observation group, 1.6 g, 3 times every day, 14 days. The improvement time of frequent micturition, urgent urination and odynuria, and urinary leukocyte number, urinary bacterial quantitative culture changes and the side effect before and after treatment were observed. Results The effective rate was higher in the observation group (89.3%) than in the control group (63%) (P<0.05). The improvement time of frequent micturition, urgent urination and odynuria was shorter in the observation group than in the control group (P< 0.05). The number of urinary leukocyte and urinary bacterial significantly decreased after treatment (P<0.001), and was less in the observation group than in the control group (P<0.05). The rate of side effect was low in both groups, and there was no significant difference (P> 0.05). Conclusion Qingretonglin pill can release the clinical symptoms of urinary tract infection after spinal cord injury, and improve the clearance rate of bacteria.

OBJECTIVETo explore the features of various mediastinal lymphadenopathies using computed tomography perfusion (CTP).

METHODSCTP parameters (CTPs) of the selected mediastinal nodes from 59 patients with pathology-proven malignant lymph nodes and of those from 29 patients with clinically diagnosed or pathology-proven inflammatory lymphadenopathies were collected. Patients were divided into subgroups by etiology and phase of primary disease, including different pathological malignant nodes and diverse inflammatory nodes. CTPs were defined as blood flow (BF), blood volume (BV), mean transit time (MTT), permeability (PMB), and time to peak (TTP). Differences of CTPs were compared between malignant and benign nodes, and among subgroups, respectively.

RESULTSIn the mediastinum, no significant differences of CTPs were found between malignant and benign groups (all P>0.05), the same for subgroups of malignant nodes (all P>0.05). Acute lymphadenitis had higher BF and BV than chronic inflammatory, lymphoid tuberculosis, sarcoidosis and malignant nodes. The BF of malignant nodes was markedly slower than that of acute lymphadenitis (P=0.01), but faster than chronic inflammatory nodes (P=0.04) and sarcoidosis (P=0.03), with no significant difference compared with lymphoid tuberculosis. Pneumonia-complicated lymphoid tuberculosis showed the longest MTT while sarcoidosis displayed the shortest MTT, and inflammatory nodes, lymphoid tuberculosis without complicated pneumonia and malignant nodes had moderate MTT.

CONCLUSIONCTPs show promising potential in distinguishing various lymphadenopathies in the mediastinum, but more studies are needed to improve their specificity.

Adult ; Female ; Humans ; Lymph Nodes ; diagnostic imaging ; Lymphatic Diseases ; diagnostic imaging ; Male ; Mediastinal Diseases ; diagnostic imaging ; Perfusion ; Retrospective Studies ; Tomography, X-Ray Computed ; methods

Objective　To examine the left ventricular diastolic function of patients with coronary artery diseases by noninvasive echocardiography and invasive coronary angiography.Methods　Mitral and pulmonary venous Doppler signals were recorded by the continuous wave Doppler technique in 62 patients, both coronary angiography and left ventricle pressure were measured. Patients were divided into normal and abnormal groups based on coronary angiography.Results　Left ventricular end diastolic pressure (LVEDP) (17.6 mm Hg vs 13.2 mm Hg,P<0.05),the left ventricular negative Δp/Δt before atria contraction ［-(224.63±52.18),-(87.81±24.41),P<0.05］,and the elevated value of left ventricular pressure derived from atria contraction ［(6.66±0.92)mm Hg vs (0.96±1.87)mm Hg,P=0.0094］ in abnormal group were more than those in the normal. The ratio of slope in pulmonary vein and mitral A wave peak was less in normal group than that in the abnormal (5.10±0.31 vs 4.12±0.20,P＝0.0106). But the isovolume relaxation parameters had no differences between the two groups.Conclusions　Coronary arter ischemia is an important cause of left ventricular impaired relaxation aggravation and stiffness, the diastolic abnormalities existed in the whole diastolic duration. Except ischemia, other factors also could cause relaxation disfuction and even elevate the LVEDP.

Objective To explore the influence of lung IL-10 on interstitial dendritic cells in multiple organ dysfunction syndrome(MODS),and its role in immunological dysfunction.Methods The model of MODS was replicated by injecting zymosan into the peritoneal cavity of C57BL/6 mice.The mice were then randomly divided into normal groups as well as the groups of 3-6 hours,12-48 hours,5-7 days and 10-12 days post trauma.The level of CCR7 mRNA in lung was detected by RT-PCR and the proteins of CCR7 and IL-10 were immunohistochemically stained.The number of mononuclear cells in MHC-II positive peripheral blood was determined by flow cytometry.Results At the early stage of injury(3-6 hours),the number of IL-10 positive cells and the expression of CCR7 increased,but the number of mononuclear cells in MHC-II positive peripheral blood decreased.At the stage of 12-48 hours,the number of IL-10 positive cells and the expression of CCR7 continued to increase obviously,and that of mononuclear cells in MHC-II positive peripheral blood went on decreasing.At the stage of 10-12 days,the number of IL-10 positive cells increased dramatically compared with that in normal group,but the expression of CCR7 declined obviously in contrast to that in 12-48 hours,and the number of mononuclear cells in MHC-II positive peripheral blood decreased to the lowest level.Conclusions The expression of IL-10 in lung could inhibit the excessive immunological damage induced by dendritic cells on the one hand,and it probably leads to immunosuppression by reducing the CCR7 expression of dendritic cells and the number of mononuclear cells in MHC-II positive peripheral blood on the other hand.

2(38.2 vs 34.4 months, P=0.214).The 1, 2 and 3-year local control rates were 84.0%, 72.9% and 56.7%, respectively.Conclusion:When given a sufficient combination of modality treatment for selected elderly patients with esophageal carcinoma, the tolerance and therapeutic efficacy were not significantly worse than those of younger patients.Therefore, combined chemoradiotherapy for elderly patients is worthy of further study.

With the analyses of correlative techniques, such as ODBC and ASP, and the application characteristics of HIS, this paper develops an object-oriented design project and its application system.