Objective To compare the fatigue characteristics between people with fatigue-predominant sub-health and patients with ulcerative colitis.Methods Fatigue Self-assessment Scale (FSAS) was applied in people with fatigue-predominant sub-health and patients with ulcerative colitis.By using the multivariate statistical analysis,the fatigue characteristics of them were analyzed.Results The fatigue characteristics of two groups of people were different.The scores of physical fatigue,mental fatigue,fatigue consequences,total fatigue,reaction of fatigue after sleep and rest,situation of fatigue in people with fatigue-predominant sub-health were 31.06 ± 17.08,24.63 ± 14.50,24.38±13.39,26.36 ± 11.97,30.63 ± 22.70,42.89 ± 21.47,and in patients with ulcerative colitis were 25.60 ± 22.98,17.80 ± 18.44,20.54 ± 18.53,21.20 ± 17.15,18.18 ± 25.47,41.21 ± 25.70.There were statistical differences on the two groups (P ＜ 0.05 ).There were statistical differences between the percents of four degrees ( unconspicuous,mild,moderate,heavy) of physical fatigue,mental fatigue,fatigue consequences between in people with fatigue-predominant sub-health and in patients with ulcerative colitis (P ＜ 0.05 ).There were statistical differences on the percents of five degrees ( no obvious,slightly,less obvious,obvious,very obvious) of unrelieved fatigue after sleep and rest,situation of fatigue between in people with fatigue-predominant sub-health and in patients with ulcerative colitis (P ＜ 0.05 ).Conclusion The type,severity of fatigue,and characteristics ( such as unrelieved fatigue after sleep and rest,situation of fatigue) are different between people with fatigue-predominant sub-health and patients with ulcerative colitis.