1.Study on the Immune Efficiency for General Vaccine Against Avian Influenza Virus Using Human Mycobacterium Tuberculosis hsp70 as the Carrier for Peptide Epitopes
Qi-Sheng ZHENG ; Gong-Bao XU ; Hong-Yan HOU ; Xue-Hua ZHANG ; Ji-Bo HOU ;
China Biotechnology 2006;0(12):-
M2e gene of three copies for H5N1 subtype AIV was synthesized and fused with human mycobacterium tuberculosis hsp70 gene.The fused gene was cloned into the prokaryotic expression vector to get pET-3M2e and pET-3M2e-hsp70.Recombinant protein r3M2e and r3M2e-hsp70 were successfully expressed induced with IPTG and purified with Ni2+-NTA collumn.Following that,the immunity of the recombinant protein was analysized with Western blot.20-day-old AIV non-immunized chickens were vaccined with r3M2e and r3M2e hsp70,at the same time,Trx and KLH-M2e inoculated chickens were served as vector and positive controls.Two weeks after the primary vaccination,every group was boosted with the same vaccine as in the primary vaccination.The humoral immunity of the vaccined chickens was evaluated with antibody detection against M2e,cytopathic suppression test,and indirect fluorescence assay.The cellular immunity was estimated according to lymphocyte subtype analysis with flow cytometry and M2e specific cytokine detection.Four weeks after the boost vaccine,all groups were challenged with 100EID50 AIV of H9N2 subtype,and the virus from swabs was detected with Real-time PCR.Results indicated that r3M2e hsp70 vaccined chicken developed the better humoral and cellular immune response,also,made a better performance compared with r3M2e vaccined group in virus challenge.
2.Effect of different sex hormones on testicular descent and their relation to expression of INSL3 mRNA in rats
En XU ; Wei-Jue XU ; Bao-Wang ZHOU ; Jian-Wen CHEN ; Yu-Hua XIA ; Meng-Ying QIAN ; Daixian GONG ; Su YAN
Chinese Journal of Endocrinology and Metabolism 2000;0(06):-
Objective To observe the effects of estradiol benzoate (E_2),human cberionic gonadotropin (hCG) and luteinizing hormone-releasing hormone (LHRH) on testicular descent and their relation to the expression of insulin-like factor-3 (INSL3) mBNA in rats.Methods At birth,40 SD male rats were assigned to 4 groups,and each group consisted of 10 rats.Each group received injection of one of the following drugs:normal saline(NS),E_3,hCG+E_2,and LHRH+E_2 for 29 clays after birth.Total RNA was extracted from the testis on day 30 after birth.INSL3 was synthesized and further amplified by RT-PCR.Results (1) Testicular descent rate on clay 30 after birth was 100% (20/20) in the group of NS,0% (0/20) in the group of E_2,85% (17/20) of hCG + E_2,and 70% (14/20) of LHBH + E_2.There was no significant difference between the last two groups ( P = 0.45).(2) Weight of testis differed significantly among the groups ( F = 56.67,P<0.01 ),being lightest in the group of E_2.(3) E_2 could damage the germ cells of testis,however hCG and LHRH ameliorated this situation. (4) The expression of INSL3 mBNA was absent in the group of E_2,while the other groups showed normal expression.Conclusion E_2 inhibits testicular descent in rats,while hCG and LHRH promote the progress.The mechanism seems to be related to the expression of INSL3 mRNA.
3.Coexistence of Pancreatic Carcinoma and Pancreatic Tuberculosis: Case Report.
Zhen Jiang ZHENG ; Hao ZHANG ; Guang Ming XIANG ; Jun GONG ; Gang MAI ; Xu Bao LIU
Gut and Liver 2011;5(4):536-538
Pancreatic tuberculosis (TB) is extremely rare and mimics pancreatic carcinoma both clinically and radiologically. This paper discusses the occurrence of 2 heterogeneous masses located in the head and tail of the pancreas in an adult male. In this patient, laparotomy was performed because of the high suspicion of pancreatic carcinoma. Intraoperative fine needle aspiration biopsy revealed the coexistence of pancreatic carcinoma with pancreatic TB, and a combined resection of the distal pancreas and spleen was successfully performed. Following surgery, the patient received standard chemotherapy for TB. At 7-month follow-up, computed tomography showed resolution of the mass in the pancreatic head. Clinicians must maintain a high index of suspicion for pancreatic TB in patients with pancreatic masses. The coexistence of malignancy and TB should be considered when patients present with multiple pancreatic masses.
Adult
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Biopsy
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Biopsy, Fine-Needle
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Follow-Up Studies
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Head
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Humans
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Laparotomy
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Male
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Pancreas
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Pancreatic Neoplasms
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Spleen
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Tuberculosis
4.Pancreatic Panniculitis Associated with Acinar Cell Carcinoma of the Pancreas: A Case Report.
Zhen Jiang ZHENG ; Jun GONG ; Guang Ming XIANG ; Gang MAI ; Xu Bao LIU
Annals of Dermatology 2011;23(2):225-228
Pancreatic panniculitis is a rare type of disorder associated with pancreatic diseases. We describe here a case of 54-year-old man who was admitted to the Department of Dermatology with the diagnosis of erythema nodosum. The patient presented with a 9-month history of painful erythematous nodules on the extremities, joint pain and swelling, and weight loss. A highly elevated level of pancreatic lipase was found on the laboratory examinations. The biopsy specimens from the skin lesions showed subcutaneous fat necrosis. Abdominal computed tomography (CT) revealed a large mass with central necrosis in the body and tail of the pancreas. Distal pancreatectomy, splenectomy and partial transverse colectomy were successfully performed on day 17 of the hospitalization. The histopathologic findings supported the diagnosis of acinar cell carcinoma of the pancreas (ACCP). Postoperatively, the level of serum lipase returned to normal, and the skin lesions and joint manifestations gradually regressed. However, the swelling did not significantly resolve in the left knee. In view of the non-specific clinical presentation of this disease, clinicians should be alert and have a high index of suspicion for pancreatic panniculitis.
Acinar Cells
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Arthralgia
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Biopsy
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Carcinoma, Acinar Cell
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Colectomy
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Dermatology
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Erythema Nodosum
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Extremities
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Hospitalization
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Humans
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Joints
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Knee
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Lipase
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Middle Aged
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Necrosis
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Pancreas
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Pancreatectomy
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Pancreatic Diseases
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Panniculitis
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Skin
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Splenectomy
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Subcutaneous Fat
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Weight Loss
5.Awn needle therapy on the impact of urinary retention after spinal cord injury.
Ren-Fu QUAN ; Rong-Liang CHEN ; Shi-Chao XU ; Fei QU ; An GONG ; Zong-Bao YANG
China Journal of Orthopaedics and Traumatology 2013;26(1):54-58
OBJECTIVETo investigate the therapeutic mechanism of point 8L54-through-point ST2S acupuncture with elongate needles in treating urinary retention after spinal cord injury.
METHODSThirty-five healthy Japanese rabbits (weight 2.5 +/- 0.25 kg, 3 months old, male or female), used as experimental subjects, were divided into 4 groups involving blank group, model group, acupuncture group, elongate needles group (5 rabbits in blank group, 10 rabbits in each of the other group 10). Modified Allen's method was used to cause spinal cord injury model for the model group, the acupuncture group and the elongate needles group, the blank group was only sham surgery. Immediately after spinal cord injury model,the elongated needle group was given the bilateral acupoints of the elongated needle penetration needling and electric stimulation, electrical stimulation 15 mm stimulation frequency of 20 to 40 times/mm, intensity 1.5 to 3 V the acupuncture group immediately was given ordinary electric needle acupuncture, given other disposals as the elongated needle group; model group was not given electric needle acupuncture, waiting 15 minutes, started to detect and record the observed indicator as the elongated needle group; the blank group was only sham surgery. The following indicators including the Tarlov score ,urinary bladder pressure point, the threshold urination, voided volume, were observed in the first day and the fifth day after surgery.
RESULTSThree groups of animals which modeled were urinary retention after operation. Compared to 1 d, Tarlov score of model group, acupuncture group and elongate needles group significantly improved at postoperative 5 d (P < 0.05). Compared with acupuncture group, the Tarlov score of elongate needles group had no significant difference at postoperative 5 d (P > 0.05). Compared with model group, values of urinary bladder pressure point decreased with varying degrees in the acupuncture group and elongate needles group at postoperative 5 d (P < 0.05); relative to the acupuncture group, the point of the bladder voiding pressure in the elongate needles group decreased more significantly at postoperative 5 d (P < 0.05); compared with model group, micturition threshold of acupuncture group and elongate needles group had been significantly improved at the fifth day after surgery (P < 0.05); relative to the acupuncture group, the improvement is more obvious in the elongate needles group. Compared with model group, voided volume of acupuncture group and elongate needles group had increased significantly at the fifth day after surgery (P < 0.05); relative to the acupuncture group, the increment is more evident in the elongate needles group (P < 0.05).
CONCLUSIONTo treat urinary retention after spinal cord injury, the method, which was point-through-point acupuncture with elongate needles in the "Zhibian" and "Shuidao", was better than general acupuncture method. The therapeutic mechanism may be related to stimulate peripheral nerve of bladder, and to rectify the dysfunction between detrusor and sphincter.
Acupuncture Points ; Acupuncture Therapy ; Animals ; Electroacupuncture ; Female ; Male ; Rabbits ; Spinal Cord Injuries ; complications ; Urinary Retention ; etiology ; therapy
6.The understanding of cancer pain by medical doctors and its therapeutic status in Shanghai-a survey in 2007
Peng PENG ; Jiejun WANG ; Zhezhou HUANG ; Kai GU ; Chunfang WANG ; Yongmei XIANG ; Pingping BAO ; Yangming GONG ; Chunxiao WU ; Defeng XU ; Ying ZHENG
Tumor 2009;(12):1158-1162
Objective:To investigate the understanding of cancer pain by medical doctors and its therapeutic status in Shanghai. Methods:We recruited 1 982 medical physicians from 106 secondary and tertiary hospitals in Shanghai city from January to March in 2007. The understanding and the therapeutic status of cancer pain were investigated by conducting a questionnaire survey and the results were statistically analyzed.Rusults:There were 52.3% physicians who believed that they understand the treatment protocol of the Three-step Analgesic Ladder regimen. The rate increased by 21.6 percentage point compared with the results in 1999. The understanding degree of oncologists was significantly better than non-oncologists. There were 16.8% physicians giving the correct answers on the three main principles of the dosage titration in analgesic management. The correct rate of oncologists was significantly higher than that of non-oncologists. Opium with strong analgesic effect had become the first choice for severe cancer pain, and the use of pethidine (Dolantin) for severe cancer pain decreased significantly.The pain was significantly relieved in 74.8% patients. The pain-relieving rate increased by 25.4 percentage point compared with the results in 1999. There were 81.1% medical doctors confirming the effect of pain treatment and among them oncologists were more optimistic to therapeutic outcome than others. There were 93.3% physicians who agreed that it was necessary to carry out the standardized training of cancer pain treatment and the requirement of non-oncologists were much emergent. The over-strict controls by the hospitals and the regulations were the main obstacles for using analgesic drugs.Conclusion:The understanding of medical doctors and the diagnostic and therapeutic levels of cancer pain were improved significantly compared with those in 1999. But more training of cancer pain treatment are still required to publicize the standardized therapy and overcome the obstacles of using analgesic drug.
7.Tracing to the source of staphylococcus aureus isolates from ice cream.
Yan-Jun ZHANG ; Dan-Ge XU ; Ye-Zhen FANG ; Pu GONG ; Min ZHU ; Fang-Zhen BAO
Chinese Journal of Preventive Medicine 2008;42(7):499-501
OBJECTIVETo investigate the contamination of Staphylococcus aureus isolates in ice cream by phenotypic typing and molecular typing.
METHODSThe Staphylococcus aureus isolates were separated from ice cream, filler, cutter, salves and material. The separated isolates were characterized by drug-resistance, staphylococcal enterotoxin (SEA-E), SE (A-E, G-J) genes and pulsed-field gel electrophoresis (PFGE) types.
RESULTTwo Staphylococcus aureus isolates were separated, one from ice cream, another from cutter. Their characteristics of drug-resistance, staphylococcal enterotoxin (SEA-E), SE (A-E,G-J) genes and PFGE type were the same.
CONCLUSIONThe two Staphylococcus aureus isolates were the same clone. The contaminated Staphylococcus aureus isolates could be traced to the contaminated cutters.
Anti-Bacterial Agents ; pharmacology ; Bacterial Typing Techniques ; Electrophoresis, Gel, Pulsed-Field ; Enterotoxins ; genetics ; Food Microbiology ; Ice Cream ; microbiology ; Microbial Sensitivity Tests ; Staphylococcus aureus ; classification ; drug effects ; isolation & purification
8.Removal of αGal xenotransplantation antigen by a novel α-galactosidase.
Hong-Wei GAO ; Xue ZHANG ; Su-Bo LI ; Ying-Xia TAN ; Guo-Qiang BAO ; Ying-Li WANG ; Li-Juan XU ; Shou-Ping JI ; Feng GONG
Journal of Experimental Hematology 2012;20(5):1231-1234
αGal, a xenotransplantations antigen (XTA), can lead to hyper acute reaction (HAR) in xenotransplantation. α-Galactosidase from B. fragilis is a novel galactosidase belong to CAZy GH110 which can clear the terminal αGal from branched and linear oligosaccharides. This study was purposed to investigate the removal effect of a novel α-galactosidase on α-Gal XTA on surface of red blood cells. The αGal XTA from the red blood cells of cattle, pig, dog and rabbit was digested by using recombinant α-galactosidase; the α-Gal antigens on surface of cells was detected by flow cytometry. The results showed that the XTA was disappeared completely or mainly. It is concluded that the novel α-galactosidase is a potential enzyme to remove the XTA on the surface of xenotransplants and can be used to overcome the HAR in xenotransplantation.
Animals
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Antigens, Heterophile
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immunology
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Cattle
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Dogs
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Epitopes
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Erythrocytes
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immunology
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Macaca mulatta
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Mice
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Mice, Inbred BALB C
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Rabbits
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Swine
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Transplantation, Heterologous
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alpha-Galactosidase
;
immunology
9.Effect of GMCSF-absence on neovascularization during wound healing.
Yong FANG ; Sheng-Ji GONG ; Ying WANG ; Ying-Hua XU ; Shi-san BAO
Chinese Journal of Plastic Surgery 2007;23(3):233-235
OBJECTIVETo study the effect of GMCSF-absence on the rate of wound healing and neovascularization during wound repair.
METHODS30 wild type (WT) mice and 30 GMCSF- absence mice (GMCSF-KO) were obtained. They were received full thickness skin wound (0.8 cm x 0.8 cm) in each side of midline after deeply anesthesia. In the different post-injury time points, the wound sites were digitally photographed to calculate the percentage of wound closure by using computer image analyses software. The wound specimens were also obtained dynamically for immunohistological analysis of CD31 at wound site.
RESULTSThe analysis of the wound closure showed that wound healing in GMCSF-KO mice was delayed significantly comparing with that in WT mice from the day 3 post-wounding. At days 7 and 10 after wounding significantly more numbers of blood vessels were formed in the WT controls compared to the GMCSF-KO mice.
CONCLUSIONSGMCSF-absence inhibits neovascularization during wound repair and leads to the delay of wound healing.
Animals ; Disease Models, Animal ; Female ; Granulocyte-Macrophage Colony-Stimulating Factor ; deficiency ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Monocytes ; Neovascularization, Physiologic ; Skin ; blood supply ; injuries ; Wound Healing
10.Analysis of 'treating different diseases by the same method' from aspect of theoretical research and clinical application of 'replenishing Qi and strengthening Shen'.
Jing-Cheng DONG ; Jin-Feng WU ; Hong-Ying ZHANG ; Wei-Yi GONG ; Bao-Jun LIU ; Hai-lin XU
Chinese Journal of Integrated Traditional and Western Medicine 2013;33(5):695-700
Treating different diseases by the same method is one of the most important characteristics in Chinese medicine, and as the main principle of treatment it has been widely applied in Chinese clinics. Its clinical effect is clear. The integration of 'differentiation of diseases' and 'differentiation of syndrome' should be the prerequisite and basis of 'treating different diseases by the same method'. Only if different diseases have the same syndrome, the same treatment can be used on them. Replenishing qi and strengthening Shen is a widely used method that carries out 'treating different diseases by the same method'. It is indicated that the method of 'replenishing qi and strengthening Shen' has preferable effects on many diseases. Part of its mechanism is associated with the improvement of function of neuro-endocrine-immune network, and therefore, it has the clinical effect of 'adjustment of the whole and improvement of the part' on partial disorders. Asthma, chronic obstructive pulmonary disease (COPD), uterine bleeding in puberty, anovulatory infertility, Kidney syndrome and aging, although they are attributed to different diseases and states, only if they have the syndrome of Shen deficiency, the principle of 'treating different diseases by the same method' and the method of 'replenishing qi 'and strengthening Shen' can be used effectively.
Drugs, Chinese Herbal
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therapeutic use
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Humans
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Medicine, Chinese Traditional
;
methods
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Phytotherapy
;
methods