To construct gene knock-out mutant of a two-component signal transduction system named 2148hk/rr in Streptococcus suis type 2 virulent strain 05ZYH33.Recombinant gene knock-out vector was constructed consisting of Spc~r cassette with flanking homology regions to the target genes,the isogenic 2148hk/rr-deficient mutant was screened by allelic replacement.PCR analysis and Southern hybridization confirmed that the coding genes of 2148hk/rr were replaced completely by spc~r cassette.Conclusion The mutant of 05ZYH33 2148hk/rr system was successfully constructed,which laid the foundation for farther research on the role of this two-component signal transduction system during infection.

Primary gallbladder cancer is a relatively common biliarv malignant tumor but with a high mortality rate.Most patients with this disease reach an advanced stage when late onset symptoms occtur, and the prognosis tends to be poor even after radical operation.Searching for new diagnostic approaches of primary gallbladder, standardizing treatment strategy, conducting multieenter clinical trials of new drug, developing preventive measures according to different situations are significant methodls to improve the treatment effect prognosis.In this article, we reviewed the research progress of high risk factors, diagnostics, and treatment strategies of primary gallbladder cancer.

Aim To evaluate the effect of paeonol on the activity of tyrosinase and provide experimental evidence for the treatment of hyperpigmentation disorders. Methods Tyrosinase activity was estimated by measuring the oxidation rate of L-3,4-dihydroxyphenylalanine (L-Dopa). The inhibitory effects of paeonol on the activity of mushroom tyrosinase and Michaelis-Menten kinetics were deduced from the Lineweaver-Burk plots. Results The inhibitory concentration of paeonol leading to 50% enzyme Paeonol is a potential mixed inhibitor of mushroom tyrosinase. The mixed inhibition function may originate from its ability to form a Schiff base with a primary amino group and to chelate copper at the active site of tyrosinase.

Resveratrol is a polyphenolic compounds in many plants.It has many effects inchuding antioxidation,anti-inflammation,inhibiting apoptosis,and scavenging free radicals.Resveratrol preconditioning has neuroprotective effect in ischemia-reperfusion in rats by activating silent information regulator 2 homolog 1.This effect is similar to brain ischemic preconditioning.In addition,resveratrol can also attenuate the brain infraction volume of cerebral ischemia/reperfusion in mice and improve neurological function.This article reviews the neuroprotective effect of resveratrol and its mechanisms in cerebral ischemia.

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Neural Conduction ; physiology ; Paraplegia ; diagnosis ; physiopathology ; Sural Nerve ; physiopathology

Adenosine monophosphate-activated protein kinase (AMPK) is a serine/threonine protein kinase,which is a energy regulation switch in the cells of the body.In the case of nutritional deficiencies and ischemia,AMPK system is activated as the metabolism and stress signal transduction component to regulate the expression of downstream target proteins.After acute ischemic stroke,AMPK is activated and aggravates neuronal apoptosis,and giving AMPK inhibitor may reduce cerebral ischemic injury.The activation of AMPK after stroke may result in the upregulation of brain-derived neurotrophic factor expression and the activation of endothelial nitric oxide synthase,and they play the protective roles for neuronal regeneration and repair.This article reviews the advances in research on the roles of AMPK in experimental cerebral ischemia.

Purpose:To study the influence of chemotherapy on radiotherapy in locally advanced nasopharyngeal carcinoma. Methods:96 patients with histologically proven nasopharyngeal carcinoma in stages Ⅲ,Ⅳ were divided randomly into two groups. Radiotherapy group (48 cases) only received radiotherapy with DT66—76Gy in nasopharynx and DT50—70Gy in the neck. Chemoradiotherapy group (48 cases) received chemotherapy with cisplatin and 5 fluorouracil before the same radiotherapy as the radiotherapy only group. Results:The 1,3,5 year survival rates were 94.2%,72.9%,65.7% in chemoradiotherapy group and 89.1%,56.8 %,46.8% in radiotherapy group respectively, with significant difference between the two groups ( P

An artificial neural network method for quality estimation of traditional Chinese medicine was suggested, and quality of Hou-Po was estimated by the proposed method in comparison with the analytical results of gas-liquid chromatography. The successful rate reached 100%. The results showed that the neural network method is reliable, and therefore may be referred to as an effective technique for the quality estimation of traditional Chinese medicine.

Objective To investigate the effect of bezafibrate on serum pro-inflammatory cytokine levels and blood pressure change in patients with hypertriglyceridemia and hypertension after antihypertensive therapy with extended release nifedipine. Methods Fifty-eight patients with both hypertriglyceridemia and hypertension, which was treated with extended release nifedipine, were randomized into 2 groups: bezafibrate group (n=30) and placebo group (n=28). Changes in levels of blood lipids, high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor?(TNF-?) and interleukin-6 (IL-6) were observed following the treatment. A correlation between these changes and blood pressure was analyzed in both groups. Results The levels of triglyceride, total and low density lipoprotein cholesterol were significantly reduced (P

Objective To investigate the safety and efficacy of stent insertion combined with high-intensity focused ultrasound(HIFU) ablation for malignant obstructive jaundice caused by pancreatic carcinoma.Methods From January 2013 to February 2017,a total of 80 patients with malignant obstructive jaundice caused by pancreatic carcinoma underwent biliary stent insertion in our center,who were divided into combined treatment(stent insertion with HIFU ablation) group(n=38) and solitary stent insertion group(n=42).The stent patency time and survival time were compared between the two groups.Results Relief of jaundice were archived in all patients in both groups.Cholangitis was observed in 7 cases in both two groups.Reobstruction of stent was observed in 15 patients in both two groups.The median stent patency time was 179 days in combined treatment group and 119 days in solitary stent insertion group(P<0.01);the median survival time was 209 days in combined treatment group and 145 days in solitary stent insertion group,the differences were significant(P<0.01).Based on the univariate and multivariate Cox regression analyses,the predictors of prolonging survival included subsequent HIFU ablation after stent insertion and earlier tumor stage(P<0.05).Conclusion Combined biliary stent insertion and HIFU ablation is a safe and effective method for malignant obstructive jaundice caused by pancreatic carcinoma,and seems to extend stent patency time and survival when compared with biliary stent only.