No abstract available.
Glycoproteins* ; Goblet Cells*

No abstract available.
Animals ; Capsaicin* ; Goblet Cells* ; Rats*

The mucous layer located on the cornea epithelial surface is mainly composed of mucin.It is unique in being composed of a central protein back bone surrounded by an outermost dense oligosaccharide matrix, yet its pre-cise composition is still unknown. Conjunctival goblet cells have been known to express the mucin MUC5AC. We attempted to reveal the presence of the secretory mucin MUC5AC as a component of the mucous layer in the rabbit corneal tear film. An antibody to a MUC5AC peptide was used to detect the MUC5AC mucin present in the tear film by immunohistochemistry. Human conjuncti-val impression cytology and immunohistochemistry were done with the same antibody. The MUC5AC mucin was observed in the precorneal tear film and con-junctival goblet cells by immunohistochemistry.The MUC5AC mucin is a component of the mucous layer and is produced by the goblet cells of con-junctiva.
Cornea ; Goblet Cells ; Humans ; Immunohistochemistry ; Mucins ; Tears*

PURPOSE: To evaluate the immediate effects of 3% diquafosol ophthalmic solution on tear MUC5AC concentration and corneal wetting property in rabbit eyes. METHODS: Six New Zealand white rabbits were used in the present study. Fifteen minutes after instilling 50 microL of 3% diquafosol into the right eye of each rabbit and 50 microL of saline into the left eye, corneal wetting property, tear MUC5AC concentrations and the area of periodic acid-Schiff (PAS)-stained conjunctival goblet cells were evaluated under general anesthesia using conjunctival impression cytology. Corneal wetting property was evaluated by measuring the duration from when the image of the microscopic light beam was clear on the corneal surface immediately after blinking to when the image began to blur. RESULTS: The mean time of corneal wetting property was 86.40 (+/- 17.90) seconds in the diquafosol group and 49.00 (+/- 6.35) seconds in the control group. There was a significant difference between the two groups (p = 0.043). The mean concentration of tear MUC5AC was significantly higher in the diquafosol group (18.21 +/- 1.52 ng/mL) than the control group (12.75 +/- 1.82 ng/mL; p = 0.028). Conjunctival impression cytology showed the area of PAS-stained conjunctival goblet cells was significantly lower in the diquafosol group (23.17 +/- 0.05%) than the control group (32.49 +/- 0.08%; p = 0.028). CONCLUSIONS: Immediately after instilling 3% diquafosol, corneal wetting property improved significantly. Also tear MUC5AC concentration, which was released from conjunctival goblet cells increased compared to saline.
Anesthesia, General ; Blinking ; Goblet Cells ; Rabbits* ; Tears

PURPOSE: To evaluate the characteristics of MUC2, MUC5AC expression and the effect of beta-blocker on MUC2, MUC5AC expression in cultured human conjunctival cell. METHODS: Human conjunctival cell was cultured. After obtaining the monoclonality of conjunctival cells, secondary culture was done. Cultured conjunctival cell was treated with 0.2 nM timolol. Specimen was collected in 1, 3, 5, 10 days after the confluence of cultured conjunctival cells. To determine the effect of beta blocker, Periodic acid-Schiff (PAS) staining, immunohistochemistry, RT-PCR, and flowcytometry were performed. RESULTS: Goblet cell was found in cultures of conjunctival cell. MUC5AC was detected in RT-PCR, immunohistochemistry, and flowcytometry, but MUC2 was detected only in flowcytometry. Beta blocker didn't have significant effects on expression of MUC2 and MUC5AC in flowcytometry. CONCLUSIONS: MUC2 and MUC5AC were detected in cultured conjunctival cell. Beta blocker may not affect goblet cell. The other factor will be related to goblet cell suppressing the mucin in long standing antiglaucomatous medication.
Goblet Cells ; Humans* ; Immunohistochemistry ; Mucins ; Timolol

No abstract available.
Animals ; Goblet Cells* ; Rats* ; Salt Gland*

Humans ; Goblet Cells ; Stomach Neoplasms ; Carcinoma

In this study, we investigated whether sphingosine-1-phosphate, furosemide, and indomethacin affect mucin release from airway goblet cells. Confluent primary hamster tracheal surface epithelial cells were metabolically radiolabeled and chased for 30 min or 24 hr in the presence of varying concentrations of the above agents to assess the effects on 3H-mucin release. Sphingosine-1-phosphate stimulated mucin release during 30 min of treatment period in a dose-dependent manner. However, furosemide and indomethacin showed no effect on both basal and stimulated mucin release during 30 min or 24 hr of treatment period. We conclude that sphingosine-1-phosphate can affect mucin release by directly acting on airway mucin-secreting cells.
Animals ; Cricetinae ; Epithelial Cells ; Furosemide* ; Goblet Cells* ; Indomethacin* ; Mucins*

The authors evaluated whether cataract surgery developed ocular surface changes or not. Fifty three cataract patients were divided into 2 groups according to the method of subconjunctival injection at the end of the operation. In the first group, betamethason was injected in the lower nasal quadrant and gentamicin was injected in the lower temporal quadrant. In the second group, both betamethason and gentamicin were injected together in the lower nasal quadrant. The tear film break-up time and Schirmer test results were reduced significantly after cataract surgery in both groups. Goblet cell numbers were reduced significantly on the 12 o'clock, lower temporal quadrant and lower nasal quadrant conjunctiva, and especially that of the lower quadrant, in which gentamicin was injected, was reduced more. Instilled drug before and after the operation, electric cauterization, conjunctival incision, and subconjunctival injection of drugs, such as gentamicin and betamethason, may induce the precorneal tear film instability.
Cataract* ; Cautery ; Conjunctiva ; Gentamicins ; Goblet Cells ; Humans ; Tears

PURPOSE: To evaluate the changes in tear film and the ocular surface before and after punctal occlusion by silicone punctal plug insertion in dry eye patients. METHODS: Twenty-four eyes of 19 patients with dry eye were included. We performed corneal sensitivity test, tear film break-up time (BUT), basal tear secretion test (BST), fluorescein staining, tear clearance test (Tcr), and conjunctival impression cytology and then compared the measurements before and after punctal occlusion. RESULTS: Before silicone pucntal plug insertion corneal sensitivity, BUT, BST, fluorescein staining score, and Tcr were: 52.50+/-6.55 mm, 4.47+/-1.33 sec, 4.07+/-1.86 mm, 4.74+/-3.21 and 3.83+/-1.62, respectively. The grade of conjunctival squamous metaplasia was 2.45+/-1.26, and goblet cell density was 131.82+/-125.53 cells/mm2 before punctal occlusion. After punctal plug insertion corneal sensitivity was 58.13+/-3.72 mm (p=0.015), BUT was 6.76+/-2.59 sec (p<0.01), BST was 5.57+/-3.32 mm (p=0.161), fluorescein staining score was 1.74+/-2.13 (p<0.01) and Tcr was 4.50+/-1.29 (p=0.198). The degree of conjunctival squamous metaplasia was 1.12+/-0.83 (p=0.019) and goblet cell density was 206.67+/-155.78 cells/mm2 (p=0.229) after punctal plug insertion. CONCLUSIONS: The tear films and ocular surface changes after punctal plug insertion in dry eye patients include increased corneal sensitivity, improved quality of tear films and decreased conjunctival squamous metaplasia.
Dry Eye Syndromes* ; Fluorescein ; Goblet Cells ; Humans ; Metaplasia ; Silicones* ; Tears