No abstract available.
Cartilage* ; Glutaral* ; Rabbits* ; Transplants*

The test was performed on 226 tuberculosis patients of different forms and 119 healthy controls and patients of other diseases. The results showed high percentage of tuberculosis patients having positive reaction, different significantly from healthy controls and patients with leprosy and lung cancer (71,43-100% versus 0-4%). Together with ELISA to detect IgG anti M.tuberculosis, glutaraldehyde test showed to have sensitivity of 84-87% in extrapulmonary cases, 89-100% in pulmonary tuberculosis and specificity about 94% when compared with final clinical diagnosis and data obtained from healthy controls
Tuberculosis ; Glutaral ; diagnosis

Glutaraldehyde is a kind of volatile and irritating aldehyde organic compound, which belongs to high-efficiency disinfectant. It has a strong stimulating effect on the mucous membranes of the eyes, respiratory tract and digestive tract, and skin causing denaturation, liquefaction and necrosis of mucous membrane proteins. This article analyzes the treatment process of a patient with high-concentration glutaraldehyde poisoning by oral and inhalation, and discusses the clinical manifestations and prognosis of high-concentration glutaraldehyde poisoning, so as to provide a basis for clinical treatment.
Administration, Inhalation ; Aldehydes ; Glutaral ; Humans ; Respiratory System

Female epispadias is an uncommon congenital anomaly in genitourinary tract. We experienced a case of female epispadias with total urinary incontinence which was improved with periurethral injection of Glutaraldehyde Cross-Linked Collagen(GAX-Collagen) into the area of the bladder neck. The procedure was simple to perform and without significant complications. Herein we report a case of female epispadias in 29-year old female.
Adult ; Epispadias* ; Female* ; Glutaral ; Humans ; Neck ; Urinary Bladder ; Urinary Incontinence

We investigated the effect by the chemical fixative on human fibroblast cells (HFCs) in order to make nano-scale images using by the atomic force microscopy (AFM). The cell fixation needed to be optimized as prerequisite step for the preparation before analysis. AFM imaging after optimal wet fixation can provide practical, simple and fast technique for scanning living cells. In this study, AFM images - topography and amplitude - and the optic images of HFCs which were fixed with phosphate buffered saline (PBS), 2:1 ethanol:acetic acid, 4% glutaraldehyde and 37% formaldehyde were compared respectively. The final effect by washing with PBS or distilled water (D.W.) was examined after 4% glutaraldehyde fixation. To determine the optimal fixation method for HFCs, we performed quantitative and qualitative analysis by the height profile, the presence of artifacts and the morphology of well-conserved fibroblastic topography image by AFM. From AFM image which showed fibroblastic cellular morphology and differential height value of cytoplasm (670+/-47 nm, n=10) and nucleus (847+/-32 nm, n=10) in HFCs, we proposed that wet fixation by 4% glutaraldehyde, followed by final washing with PBS, could be the most suitable preparation for AFM imaging of HFCs, which enable us to approach easily on living cells with the least shrinkage.
Artifacts ; Cytoplasm ; Fibroblasts ; Formaldehyde ; Glutaral ; Humans ; Microscopy, Atomic Force ; Water

BACKGROUND/AIMS: Safety of endoscopic procedures has been a major issue over the last 10 years. Most endoscopy units use 2% glutaraldehyde and automated endoscope reprocessors (AERs) for disinfecting gastrointestinal endoscopes. We attempted an in-use evaluation of the current reprocessing procedures. METHODS: Thirty flexible endoscopes were randomly collected just after upper endoscopic examinations and were disinfected using 2% glutaraldehyde in an AER. Cultures were taken from biopsy channels (S-1), tip of the insertion tubes (S-2), umbilical cords (S-3), and angulation knobs (S-4). RESULTS: In 63.3% (19/30) of endoscopes, there was no microbial contamination after disinfection procedures. The culture positive rates of S-1, S-2, S-3, and S-4 samples were 20.0%, 0.0%, 3.3%, and 20.0%, respectively. Microorganisms of 13 species were identified, but there was no pathogen related with reported infectious complications after endoscopic procedures. CONCLUSIONS: Current disinfection procedure using 2% glutaraldehyde and an AER appears to be very effective in decontaminating patient-used endoscopes. Low level microbial contamination of endoscopes after conventional reprocessing methods may not impose great risk on patients.
Biopsy ; Disinfection* ; Endoscopes* ; Endoscopes, Gastrointestinal ; Endoscopy ; Glutaral* ; Humans ; Umbilical Cord

We investigated the long-term scleral toxicity after single intraoperative exposure to mitomycin-C(MMC) at 2 different exposure length of time and with or without profuse irrigation after the MMC applications during glaucoma filtering surgery(GFS) in rabbit eyes. Albino rabbits undergoing GFS received a single exposure to MMC 0.5 mg/ml for 1 or 5 minutes with placing MMC-soaked sponges between the conjunctival flap and the scleral bed. A subsequent irrigation of the exposed tissue with 250 ml of balanced salt solution(BSS) was done in one group but not in the other groups. Six months after GFS, the eyes were enucleated and fixed in glutaraldehyde for transmission electron microscopy(TEM). TEM revealed degenerative changes of the fibroblasts at the middle portion of sclera in all MMC treated groups. There were some differences between the groups with 1 minute and 5 minutes exposure to MMC. No discernible differences existed in the ultrastructural findings of the scleral fibroblasts between the groups with or without BSS irrgation after 5-minutes exposure to MMC. Our observation suggest that an exposure to MMC 0.5 mg/ml can cause longterm scleral fibroblast toxicity. There was no discernible differences in scleral fibroblast toxicity between the groups with and without profuse BSS irrigation.
Fibroblasts* ; Filtering Surgery* ; Glaucoma* ; Glutaral ; Mitomycin* ; Porifera ; Rabbits ; Sclera

We evaluated histopathologically the effect of the viscoelastic substance on surgery of the extraocular muscle in the 26 rabbits with 1. 6-2. 5kg of weight. The viscoelastic substances were 1% sodium hyaluronate(Healon) and 2% hydroxypropyl methylcellulose(Methocel), which were introduced between the extraocular muscle and its adjacent tissue on surgery. The rabbits were categorized by group 1(the rabbits with Healon), group 2(with Methocel), and group 3(with normal saline as control). These rabbits were enuclated at 3rd, 7th, 14th, 28th and 56th day following operations, and the eyes were fixed in 2.5% glutaraldehyde solution. The tissue was stained with hematoxylin-eosin and Masson's trichrome, and examined under a light microscope. The results were as follows: 1. In the first group(Healon), the tissue adhesion and degeneration of the muscle were milder than those of control(saline) group. 2. The changes in second group(Methocel) was milder than in the first group and more severe than in control group. 3. It is concluded that 2% Methocel can be used clinically in the surgery of the extraocular muscle.
Glutaral ; Methylcellulose ; Muscles* ; Rabbits ; Sodium ; Tissue Adhesions ; Viscoelastic Substances

The discovery of an ideal technique for sterilising contaminated respirators and other anesthesia equipment remains a major problems, The antimicrobial activities of a recently discovered disinfectant alktaline-glutaraldehyde(Cidex), studied in vitro against various species of bacteria and fungi. The antimicrobial activity tests were performed according to the modified Kolmer method. The testing organisms were cultured in broth media at 37 degrees C and 25 degrees C for 18 hours to 14 days, and the disinfectant was diluted with sterile distilled;water to 0.4% and 2.0%. One milliliter of cultured broth was transferred into disinfectant-containing media and after 1, 2, 5, 10, 20, 30 and 60 minutes, one loopful of the mateials was removed from the media and inoculated into the broth media. All of the subcultures were incubated at 37 degrees C for 24 hours and fungal subcultures were incubated at 25 degrees C for 14 days. Results were obtained as follows: 1) Most of the bacteria were completely growth-inhibited by treatment with 0.4% active alkaline-glutaraldehyde solution for 2 minutes except a few strains such as St. aureus, B. subtilis and M. tuberculosis, which required from 16 to 20 min. 2) Mycobacterium tuberculosis was relatively resistant but it could be growth-inhibited by treatment with 2.0% solution for 2 minutes. 3) Growth inhibiting of fungi could be obtained by treatment with 2.0% solution for 5 to 10 minutes.
Anesthesia ; Bacteria ; Fungi ; Glutaral* ; Mycobacterium tuberculosis ; Tuberculosis ; Ventilators, Mechanical

BACKGROUND: The chemical modification of RBC surface antigen has many advantages for safe transfusion practice. We evaluated the change of antibody reactivity to RBC surface antigen before and after glutaraldehyde crosslinking. MATERIALS AND METHODS: The 10 mL of blood were collected from 20 volunteers and were treated by 2-3% glutaraldehyde at 4degrees C. After 30 minute incubation, Agglutinability of various RBC surface antigen (ABO, Rh-C, c, D, E, e) was measured by titration using anti-sera (Green Cross, Korea, Dade, USA), and compared the agglutinability changes before and after glutaraldehyde crosslinking. RESLUTS: The agglutinability of Rh surface antigens (D, C, c, E, e) was disappeared after glutaraldehyde crosslinking. However, ABO antigens (n=20) still showed strong agglutinability against antisera with some decreased. CONCLUSIONS: It would be useful to apply glutaraldehyde crossliked RBCs for rare blood group transfusion practice, if the safety problem were solved.
Antigens, Surface* ; Blood Substitutes ; Glutaral* ; Immune Sera ; Korea ; Volunteers