No abstract available.
Cartilage* ; Glutaral* ; Rabbits* ; Transplants*

The test was performed on 226 tuberculosis patients of different forms and 119 healthy controls and patients of other diseases. The results showed high percentage of tuberculosis patients having positive reaction, different significantly from healthy controls and patients with leprosy and lung cancer (71,43-100% versus 0-4%). Together with ELISA to detect IgG anti M.tuberculosis, glutaraldehyde test showed to have sensitivity of 84-87% in extrapulmonary cases, 89-100% in pulmonary tuberculosis and specificity about 94% when compared with final clinical diagnosis and data obtained from healthy controls
Tuberculosis ; Glutaral ; diagnosis

Glutaraldehyde is a kind of volatile and irritating aldehyde organic compound, which belongs to high-efficiency disinfectant. It has a strong stimulating effect on the mucous membranes of the eyes, respiratory tract and digestive tract, and skin causing denaturation, liquefaction and necrosis of mucous membrane proteins. This article analyzes the treatment process of a patient with high-concentration glutaraldehyde poisoning by oral and inhalation, and discusses the clinical manifestations and prognosis of high-concentration glutaraldehyde poisoning, so as to provide a basis for clinical treatment.
Administration, Inhalation ; Aldehydes ; Glutaral ; Humans ; Respiratory System

The discovery of an ideal technique for sterilising contaminated respirators and other anesthesia equipment remains a major problems, The antimicrobial activities of a recently discovered disinfectant alktaline-glutaraldehyde(Cidex), studied in vitro against various species of bacteria and fungi. The antimicrobial activity tests were performed according to the modified Kolmer method. The testing organisms were cultured in broth media at 37 degrees C and 25 degrees C for 18 hours to 14 days, and the disinfectant was diluted with sterile distilled;water to 0.4% and 2.0%. One milliliter of cultured broth was transferred into disinfectant-containing media and after 1, 2, 5, 10, 20, 30 and 60 minutes, one loopful of the mateials was removed from the media and inoculated into the broth media. All of the subcultures were incubated at 37 degrees C for 24 hours and fungal subcultures were incubated at 25 degrees C for 14 days. Results were obtained as follows: 1) Most of the bacteria were completely growth-inhibited by treatment with 0.4% active alkaline-glutaraldehyde solution for 2 minutes except a few strains such as St. aureus, B. subtilis and M. tuberculosis, which required from 16 to 20 min. 2) Mycobacterium tuberculosis was relatively resistant but it could be growth-inhibited by treatment with 2.0% solution for 2 minutes. 3) Growth inhibiting of fungi could be obtained by treatment with 2.0% solution for 5 to 10 minutes.
Anesthesia ; Bacteria ; Fungi ; Glutaral* ; Mycobacterium tuberculosis ; Tuberculosis ; Ventilators, Mechanical

We evaluated histopathologically the effect of the viscoelastic substance on surgery of the extraocular muscle in the 26 rabbits with 1. 6-2. 5kg of weight. The viscoelastic substances were 1% sodium hyaluronate(Healon) and 2% hydroxypropyl methylcellulose(Methocel), which were introduced between the extraocular muscle and its adjacent tissue on surgery. The rabbits were categorized by group 1(the rabbits with Healon), group 2(with Methocel), and group 3(with normal saline as control). These rabbits were enuclated at 3rd, 7th, 14th, 28th and 56th day following operations, and the eyes were fixed in 2.5% glutaraldehyde solution. The tissue was stained with hematoxylin-eosin and Masson's trichrome, and examined under a light microscope. The results were as follows: 1. In the first group(Healon), the tissue adhesion and degeneration of the muscle were milder than those of control(saline) group. 2. The changes in second group(Methocel) was milder than in the first group and more severe than in control group. 3. It is concluded that 2% Methocel can be used clinically in the surgery of the extraocular muscle.
Glutaral ; Methylcellulose ; Muscles* ; Rabbits ; Sodium ; Tissue Adhesions ; Viscoelastic Substances

Female epispadias is an uncommon congenital anomaly in genitourinary tract. We experienced a case of female epispadias with total urinary incontinence which was improved with periurethral injection of Glutaraldehyde Cross-Linked Collagen(GAX-Collagen) into the area of the bladder neck. The procedure was simple to perform and without significant complications. Herein we report a case of female epispadias in 29-year old female.
Adult ; Epispadias* ; Female* ; Glutaral ; Humans ; Neck ; Urinary Bladder ; Urinary Incontinence

BACKGROUND: The chemical modification of RBC surface antigen has many advantages for safe transfusion practice. We evaluated the change of antibody reactivity to RBC surface antigen before and after glutaraldehyde crosslinking. MATERIALS AND METHODS: The 10 mL of blood were collected from 20 volunteers and were treated by 2-3% glutaraldehyde at 4degrees C. After 30 minute incubation, Agglutinability of various RBC surface antigen (ABO, Rh-C, c, D, E, e) was measured by titration using anti-sera (Green Cross, Korea, Dade, USA), and compared the agglutinability changes before and after glutaraldehyde crosslinking. RESLUTS: The agglutinability of Rh surface antigens (D, C, c, E, e) was disappeared after glutaraldehyde crosslinking. However, ABO antigens (n=20) still showed strong agglutinability against antisera with some decreased. CONCLUSIONS: It would be useful to apply glutaraldehyde crossliked RBCs for rare blood group transfusion practice, if the safety problem were solved.
Antigens, Surface* ; Blood Substitutes ; Glutaral* ; Immune Sera ; Korea ; Volunteers

BACKGROUND: Bioprosthetic materials have been made using glutaraldehyde fixation of porcine or bovine pericardium during cardiovascular surgery. But these bioprostheses have the problems of calcification and mechanical failure. We determined changes in tensile strength and elasticity of pericardium after glutaraldehyde, solvent, decellularization and detoxification. MATERIAL AND METHOD: Tissues were allocated to four groups: glutaraldehyde with and without solvent, decellularization, and detoxification. We studied tensile strength and strain on tissues. We measured the tensile strength of fresh pericardium stretched in six directions (with 5 mm width), and % strain, which we calculated from the breaking point when we pulled the pericardium in two directions. RESULT: Tensile strength was reduced when we used the usual concentrated glutaraldehyde fixation (n=83, MPa=11.47+/-5.40, p=0.006), but there was no change when we used solvent. Elasticity was increased after glutaraldehyde fixation (n=83, strain (%)= 24.55+/-9.81, p=0.00), but there was no change after solvent. After decellularization of pericardium, the tensile strength was generally reduced. The decrease in tensile strength after concentrated glutaraldehyde fixation for a long time was significantly greater less than after concentrated solvent (p=0.01, p=0.00). After detoxification, the differences in strength and strain were not significant. CONCLUSION: After glutaraldehyde treatment of pericardium there is no loss in tensile strength (even though we did the glutaraldehyde, solvent and detoxification treatments LOGIC IS UNCLEAR). Also, these treatments had a tendency to increase elasticity. Although post-treatment decellularization led to a significant loss in strength, this effect could be attenuated using a low concentration of solvent or hypertonic solution.
Bioprosthesis ; Elasticity ; Glutaral ; Logic ; Pericardium ; Sprains and Strains ; Tensile Strength

We investigated the long-term scleral toxicity after single intraoperative exposure to mitomycin-C(MMC) at 2 different exposure length of time and with or without profuse irrigation after the MMC applications during glaucoma filtering surgery(GFS) in rabbit eyes. Albino rabbits undergoing GFS received a single exposure to MMC 0.5 mg/ml for 1 or 5 minutes with placing MMC-soaked sponges between the conjunctival flap and the scleral bed. A subsequent irrigation of the exposed tissue with 250 ml of balanced salt solution(BSS) was done in one group but not in the other groups. Six months after GFS, the eyes were enucleated and fixed in glutaraldehyde for transmission electron microscopy(TEM). TEM revealed degenerative changes of the fibroblasts at the middle portion of sclera in all MMC treated groups. There were some differences between the groups with 1 minute and 5 minutes exposure to MMC. No discernible differences existed in the ultrastructural findings of the scleral fibroblasts between the groups with or without BSS irrgation after 5-minutes exposure to MMC. Our observation suggest that an exposure to MMC 0.5 mg/ml can cause longterm scleral fibroblast toxicity. There was no discernible differences in scleral fibroblast toxicity between the groups with and without profuse BSS irrigation.
Fibroblasts* ; Filtering Surgery* ; Glaucoma* ; Glutaral ; Mitomycin* ; Porifera ; Rabbits ; Sclera

PURPOSE: Vinyl polyether silicone (VPES) has a different composition from other elastomeric impression materials as it combines vinyl polysiloxane (VPS) and polyether (PE). Therefore, it is important to study its properties and behavior under different test conditions. This study investigated the dimensional stability of 5 VPES consistencies when stored for up to 2 weeks, with and without using a standard disinfection procedure. MATERIALS AND METHODS: 40 discs of each VPES consistency (total 200) were made using a stainless steel die and ring as described by ANSI /ADA specification No. 19. 20 discs of each material were immersed in a 2.5% buffered glutaraldehyde solution for 30 minutes. Dimensional stability measurements were calculated immediately after fabrication and repeated on the same discs after 7 and 14 days of storage. The data was analyzed using two-way ANOVA with a significance level set at α = 0.05. RESULTS: The discs mean contraction was below 0.5% at all test times ranging from 0.200 ± 0.014 to 0.325 ± 0.007. Repeated measures ANOVA showed a statistically significant difference after 2-week storage between the disinfected and non-disinfected groups (P < .001). Although there was no statistically significant difference between the materials at the time of fabrication, the contraction of the materials increased with storage for 1 and 2 weeks. CONCLUSION: The dimensional changes of VPES impression discs after disinfection and prolonged storage complied with ANSI/ADA standard. The tested VPES impression materials were dimensionally stable for clinical use after disinfection for 30 minutes in glutaraldehyde and storage for up to 2 weeks.
Disinfection* ; Elastomers ; Glutaral ; Silicon* ; Silicones* ; Siloxanes ; Stainless Steel