1.Study on the correlation of periodontopathic microflora and gingival crevicular fluid cytokine on periodontal disease progression.
Hae Joon LEE ; Chong Pyoung CHUNG ; Soo Boo HAN ; Seong Heui SON ; Sang Mook CHOI ; Sam Pyo HONG
Journal of the Korean Society for Microbiology 1993;28(1):81-93
No abstract available.
Gingival Crevicular Fluid*
;
Periodontal Diseases*
2.Some opinions of the microbial flora in the gingival fluid and caries
Journal of Vietnamese Medicine 2001;256(2):15-17
A study aimed at the determination of the microbial flora in the gingival fluid and carries and paradontal bursal fluid. 30 persons in Dinh Dong commune, Thanh Tri district Hanoi classified as age, gender participated the study. The results have shown that there was a balance of the microbial flora in the mouth and gingival fluid and carries and paradontal bursal fluid. The imbalance occurred when gingivitis or periodontitis appeared. The highest increased microbials were anaerobic microbial and spirillum.
Gingival Crevicular Fluid
;
Dental Caries
3.Dynamics of gingival crevicular fluid volume and enzyme activities after application of orthodontic force.
Ae Ree KANG ; Hyun Mo RYOO ; Jae Hyun SUNG
Korean Journal of Orthodontics 1989;19(3):137-145
The aim of this investigation was to study the effect of orthodontic force on the flow of gingival crevicular fluid and activities of arylsulfatase and brta-glucuronidase in crevicular fluid. The material consisted of 12 persons between the ages of 13 years and 22 years and all were categorized Class I, 4-4 extraction cases Crevicular fluids were sampled from distal crevis of each canine before treatment (phase 1), after bracketing (phase 2), after application of force (phase 3) and after run out of orthodontic force (phase 4). Crevicular fluid flow did not show any significant changes during the period of treatment. The activities of arylsulfatase increased significantly after setting of orthodontic appliance without application of force, but did not show any significant difference after application of force. The activities of beta-glucuronidase increased significantly after application of orthodontic force and decreased with force deminished. These indicated that beta-glucuronidase was good indicator of bone remodelling resulted from initial orthodontic force.
Gingival Crevicular Fluid*
;
Glucuronidase
;
Humans
;
Orthodontic Appliances
4.The effect of periodontal flap surgery on Matrix metalloproteinases (MMPs) and Tissue inhibitors of matrix metalloproteinase-1 (TIMP-1) levels in gingival crevicular fluids of periodontitis patients.
Jhee Hyun KIM ; Jea Seung KO ; Hyun Man KIM ; Tae Il KIM ; Yang Jo SEOL ; Yong Moo LEE ; Young KU ; Chong Pyoung CHUNG ; Soo Boo HAN ; In Chul RHYU
The Journal of the Korean Academy of Periodontology 2005;35(1):123-132
No abstract available.
Gingival Crevicular Fluid*
;
Humans
;
Matrix Metalloproteinase 1*
;
Matrix Metalloproteinases*
;
Periodontitis*
5.Detection and measurement of interleukin-8 in peri-implant crevicular fluid.
De-rong ZOU ; Hong ZHU ; Xiao-hui QU
West China Journal of Stomatology 2005;23(4):292-294
OBJECTIVEGingival crevicular fluid (GCF) analysis of various inflammatory mediators has been investigated as a means of providing objective criteria of peri-implant tissue health. In this report, the crevicular fluid levels of interleukin-8 (IL-8), GCF volume and clinical parameters were studied.
METHODSGCF was sampled from 35 healthy and 35 inflamed sites of implantation patients. IL-8 levels were analysed using ABC-ELISA. Clinical parameters were measured, and data analysis was performed using the software package SPSS10.0.
RESULTSSignificant difference was observed between healthy implant sites and peri-implantitis sites. GCF volume was positively correlated with PD, PI, GI and MOB. The total amount of IL-8 was positively correlated with PD and GI.
CONCLUSIONThis investigation suggested that GCF volume and IL-8 cytokine may be of value as a diagnostic and prognostic marker for peri-implantitis.
Dental Implants ; Gingival Crevicular Fluid ; Humans ; Interleukin-8 ; Prostheses and Implants
6.Quantitative Assessment of Dentine Sialophosphoprotein, Aspartate Aminotransferase and Lactate Dehydrogenase in Gingival Crevicular Fluid of Teeth with Root Resorption
Noor Ayuni Ahmad Shafiai ; Rohaya Megat Abdul Wahab
Archives of Orofacial Sciences 2022;17(2):237-246
ABSTRACT
Root resorption is a shortening of root dentine which occurs physiologically in deciduous teeth. The
present study aimed to quantify dentine sialophosphoprotein (DSPP), aspartate aminotransferase (AST)
and lactate dehydrogenase (LDH) in gingival crevicular fluid (GCF) during the physiological process
of root resorption of deciduous teeth. A cross-sectional study was conducted with 25 children aged
between 4 and 10 years old. GCF was collected from the gingival sulcus using periopaper strips from the
upper first deciduous molar (n = 45). The samples were divided equally into three groups, no resorption
(R0), moderate resorption (RM) and severe resorption (RS), based on the existing radiographs taken.
The GCF samples were then analysed using an enzyme-linked immunosorbent assay (ELISA) kit
to determine the DSPP concentration levels and BioAssays System kit for AST and LDH. One-way
ANOVA was used to determine the statistical differences between the means of the DSPP, AST and
LDH concentration level in the three groups. A difference was considered significant when p < 0.05.
High concentration levels of DSPP were significantly noted in RS (p < 0.05), compared to RM and
R0. AST also portrayed significant high activity level (p < 0.05) similar to DSPP but LDH showed no
significant changes between groups (p > 0.05). The high quantification of DSPP and AST levels in
the severe and moderately resorbed roots indicated the potential use of this protein as a biomarker for
detecting moderate-severe stages of root resorption.
Root Resorption
;
Gingival Crevicular Fluid
;
Dentin
;
Aspartate Aminotransferases
;
Lactate Dehydrogenases
7.The Relationship between Periodontal Status and Alkaline Phosphatase Levels in Gingival Crevicular Fluid in Men with Hypergonadotropic Hypogonadism.
Berrin UNSAL ; Isil SAYGUN ; Ozlem DALTABAN ; Belgin BAL ; Erol BOLU
Yonsei Medical Journal 2008;49(1):71-78
PURPOSE: The aim of this preliminary study was to determine the possible relationship between alkaline phosphatase (ALP) levels in the gingival crevicular fluid (GCF) and periodontal disease in men with hypergonadotropic hypogonadism (HH). MATERIALS AND METHODS: A total of 41 patients were divided into four groups. 9 with HH and periodontitis (P/HH), 11 with HH and gingivitis (G/HH), 12 with systemically healthy and periodontally healthy (H/C) and 9 with systemically healthy and periodontitis (P/C). The clinical evaluation of patients was based on the following parameters; the plaque index (PI), gingival index (GI), probing depths (PD) and attachment level (AL). The levels of ALP in the GCF were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: No significant difference could be detected in the mean clinical parameter data between the P/HH and P/C groups (p > 0.05). The periodontitis patients in both groups (P/C and P/HH) had higher mean probing depths than the H/C and G/HH patients (p < 0.001). The concentrations and total amounts of ALP in the GCF were significantly higher in both periodontitis groups compared to healthy and gingivitis groups (p < 0.01). The serum ALP levels were significantly higher in the P/HH group when compared to the other groups (p < 0.001). CONCLUSION: The findings of this study suggested that HH could be implicated as a contributing factor to the progress of periodontal disease.
Adolescent
;
Adult
;
Alkaline Phosphatase/*metabolism
;
Gingival Crevicular Fluid/*enzymology
;
Humans
;
Hypogonadism/diagnosis/*enzymology
;
Male
;
Periodontium/*enzymology
8.Effects of resolution of inflammation for low-power CO2 laser treatment on gingivitis patients.
Hyun Jong SONG ; Byung Ock KIM ; Hyun Seon JANG
The Journal of the Korean Academy of Periodontology 2008;38(4):657-668
PURPOSE: In this study, we compared low-power CO2 laser treatment to ultrasonic scaling, which is generally approved as a power-driven mechanical instrumentation, and evaluated both of these treatments regarding their clinical effectiveness and change in the volume of in GCF. MATERIAL AND METHODS: 20 patients who had gingivitis were selected. all of patients has no systemic problems. Randomly selected, one quadrant received ultrasonic scaling only, another quadrant received ultrasonic scaling and CO2 laser irradiation, the other quadrant received CO2 laser irradiation only. Clinical parameters measured at baseline, 1 weeks, 2weeks, 4weeks and 8weeks RESULT: Pocket probing depth and clinical attachment level were not changed during study period. Gingival index of all group were improved after treatment. At 1 weeks after treatment, Gingival index of ultrasonic scaling group was only significantly different compared to control group. At 2 weeks after treatment, gingival index of all experimental group were significantly different compared to control group. At 4 and 8 weeks after treatment, gingival index of all group were increased, but experimental group were lower than control group. Sulcus bleeding index was similar to the results of gingival index. At 1 weeks after treatment, all experimental group were significantly different compared to control group and it maintained during study. At 2 weeks after treatment, sulcus bleeding index of all group were lowest during study. Gingival crevicular fluid were measured with Periotron(R) 8000(Oraflow(R) , Inc. USA). At baseline, all group were showed moderately severe condition. At 1 week after treatment, laser treatment only group was reduced quantity of gingival crevicular fluid mostly, and all group were reduced quantity of gingival crevicular fluid. At 2 weeks after treatment, all group were healty state. At 4 and 8 weeks after treament, all group were showed recurrent of inflammation, and control group was the most significantly increased. CONCLUSION: This study showed that the effects of CO2 laser treatment were similar to conventional ultrasonic scaling and this result remained longer than plaque control only. These results suggest possibility of CO2 laser treatment for altered periodontal therapy.
Gingival Crevicular Fluid
;
Gingivitis
;
Hemorrhage
;
Humans
;
Inflammation
;
Lasers, Gas
;
Periodontal Index
;
Ultrasonics
9.Comparative volumetric and clinical evaluation of peri-implant sulcular fluid and gingival crevicular fluid.
Smiti BHARDWAJ ; Munivenkatappa Lakshmaiah Venkatesh PRABHUJI
Journal of Periodontal & Implant Science 2013;43(5):233-242
PURPOSE: Peri-implant sulcular fluid (PISF) has a production mechanism similar to gingival crevicular fluid (GCF). However, limited research has been performed comparing their behavior in response to inflammation. Hence, the aim of the present study was to comparatively evaluate PISF and GCF volume with varying degrees of clinical inflammatory parameters. METHODS: Screening of patients was conducted. Based on the perimucosal inflammatory status, 39 loaded implant sites were selected from 24 patients, with equal numbers of sites in healthy, peri-implant mucositis, and peri-implantitis subgroups. GCF collection was done from age- and sex-matched dentate patients, selected with gingival inflammatory status corresponding to the implant sites. Assessment of the inflammatory status for dental/implant sites was performed using probing depth (PD), plaque index/modified plaque index (PI/mPI), gingival index/simplified gingival index (GI/sGI), and modified sulcular bleeding index (BI). Sample collection was done using standardized absorbent paper strips with volumetric evaluation performed via an electronic volume quantification device. RESULTS: Positive correlation of the PISF and GCF volume was seen with increasing PD and clinical inflammatory parameters. A higher correlation of GCF with PD (0.843) was found when compared to PISF (0.771). PISF expressed a higher covariation with increasing grades of sGI (0.885), BI (0.841), and mPI (0.734), while GCF established a moderately positive correlation with GI (0.694), BI (0.696), and PI (0.729). CONCLUSIONS: Within the limitations of this study, except for minor fluctuations, GCF and PISF volumes demonstrated a similar nature and volumetric pattern through increasing grades of inflammation, with PISF showing better correlation with the clinical parameters.
Dental Implants
;
Gingival Crevicular Fluid*
;
Hemorrhage
;
Humans
;
Inflammation
;
Mass Screening
;
Mucositis
;
Peri-Implantitis
;
Periodontal Index
10.Matrix metalloproteinases and Tissue inhibitors of matrix metalloproteinases in gingival crevicular fluids of periodontitis patients.
Sun Yun LEE ; Jae Seung KO ; Yeoun Ho JUNG ; Hyun Man KIM ; Kyoung Hwa KIM ; Byung Keun YANG ; Soo Boo HAN ; Chong Pyoung CHUNG ; Tae Il KIM ; Young KU ; Yong Moo LEE ; In Chul RHYU
The Journal of the Korean Academy of Periodontology 2004;34(1):139-148
No abstract available.
Enzyme-Linked Immunosorbent Assay
;
Gingival Crevicular Fluid*
;
Humans
;
Matrix Metalloproteinases*
;
Periodontitis*