OBJECTIVETo investigate of antiatherogenic effect and possible mechanisms of piper longuminine.

METHODThe atherosclerotic model was established by the hypercholesterol feeding rabbits. Male Mew Zealand rabbits were randomly divided into five groups: normal group, model group, the high-dose (5 mg x kg(-1) x d(-1)) and low-dose (2.5 mg x kg(-1) x d(-1) group of piperlonguminine, and simvastatin group (5 mg x kg(-1) x d(-1)). All the rabbits were fed for 60 days. Blood samples were taken from the ear edge vein of rabbits in the day before the experiment, and in the days of 20, 40 and 60 days after the experiment, respectively. All the rabbits were fasted for at least twelve hours before the blood was taken. The blood serum were analyzed for total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C). The blood serum of the 60th day were also analyzed for superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO). At last, the pathological observation of aorta and heart samples were carried out.

RESULTCompared with those in model group, the TC, TG and LDL-C levels were reduced (P < 0.05) and the HDL-C was raised in the piperlonguminine group; also, the serum SOD and NO level was raised (P < 0.05), MDA level was reduced in the piperlonguminine group (P < 0.05). Area percentage of aorta plaque was reduced (P < 0.01) in the piperlonguminine group. The aorta and heart injury was abated and coronary artery angusty extent was markedly abatement (P < 0.01). The results of observation through transmission electron microscope (TEM) indicated that the fine structure of aortal pathological degree was markedly abated.

CONCLUSIONThe piperlonguminine could inhibit the atherogenesis formation and development, which might be due to regulating the lipid metabolism and enhancing the antioxidation.

Animals ; Atherosclerosis ; blood ; chemically induced ; drug therapy ; pathology ; Cholesterol ; blood ; Cholesterol, HDL ; blood ; Cholesterol, LDL ; blood ; Dioxolanes ; pharmacology ; Male ; Malondialdehyde ; blood ; Nitric Oxide ; blood ; Rabbits ; Superoxide Dismutase ; blood ; Triglycerides ; blood

Introduction: Human beings have been using raw materials of plant and animal origin for the treatment of various diseases. Nowadays, in developed and developing countries, plant raw materials are widely used in traditional medicine, health care, and food supplements, and they are used as raw materials in pharmaceutical production, making up a large part of the world pharmaceutical market. Therefore, research activities are intensively carried out to determine the chemical composition and mineral content of plant-derived raw materials and explain the mechanism of action. Goal: Allium polyrhizum Turcz.ex Regel determination of chemical compounds macro and micro elements and amino acids Material and Methods: Allium polyrhizum Turcz.ex Regel was collected and prepared from Huvut shar Khoshun, Shili River Province, Institute of Mongolia medicine chemistry, Inner Mongolia university, Inner Mongolia Key Laboratory Medicine Chemistry. then it was made into a type with 0.075 mm particle size by a grinding machine. Result: Dete-rmining the elemental composition of plant raw materials is important for determining the therapeutic value and safety of the plant. Some elements in Allium polyrhizum Turcz.ex Regel were determined by X-ray fluorescence method. Conclusions
1. In this study, the content of 7 elements in the above-ground part of Allium polyrhizum Turcz was determined using the XRF analysis method. Of the macro and micro elements such as Ca, K, Mn, Fe, Na, Zn, and Mg, which are essential for the human body, potassium (K) 49.7 mg/kg, calcium (Ca) 45.7 mg/kg, and sodium (Na) 31.0 mg/kg are the most had a high concentration.
2. Among various amino acids involved in protein synthesis, glutamic acid 1.89 mg, aspartic acid 1.48 mg, and alanine 1.00 mg were identified.

Introduction: One of the world’s leading causes of mortality, WHO projects that diabetes will be the seventh leading cause of death in 2030. Therefore, there is absolutely need for prevention and treatment of diabetes, and scientists are keen to introduce a variety of drug and non-drug treatment methods. Goal: To examine effect of Artemisia commutata Bess aqueous extract on Wistar rats with diabetes model type II induced by STZ with high fat diet. Material and Methods: Experimental was performed in Institute of Mongolian medicine and Chemistry, Inner Mongolia University. In study, 32 Wistar rats (body weight 180-200 g, healthy) were divided into 4 groups included Normal, Model, Metformin and Artemisia commutata Bess. Type II diabetes were induced by intraperitoneal single dose STZ 56.25 mg/kg with high fat diet except for Normal group. Then Metformin group was received by oral administration at a dosage of 50 mg/kg/day and Artemisia commutata Bess group was received by oral administration at a dosage of 55 mg/kg/day during 30 days. Blood glucose (GLU), triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT) levels were tested at the end of the experiment. Pancreatic sections were stained with hematoxylin-eosin (HE). Results: The level of blood glucose was significantly increased in Model group (23.9±1.33 mmo/L) compare to Normal group (5.64±0.24 mmol/L). Oral amdinistration of Artemisia commutata Bess 55 mg/kg/ day to treated group (9.75±1.84 mmol/L), Metformin 50 mg/kg/day to treated group (9.04±2.75 mmol/L) resulted in significantly decreased of blood glucose level less Model group. Conclusion This study demonstrated that hypoglycemic effect of Artemisia commutata Bess on diabetes model type II induced by STZ with high fat diet in Wistar rats.