1.POSITIVE INOTROPIC ACTION OF DIMETHYLTRILOBINE IODIDE ON CARDIAC MUSCLE
Chinese Pharmacological Bulletin 1986;0(06):-
Dimethyltrilobine iodide (DMT) 1.5 M-M increased the contractile force of isolated guinea pig left atria. This positive inotropic action could not be an-tagonized by either propranolol, cimetidine or diphen-hydramine. DMT 1.5 nM also increased the contractile force of isolated guinea pig left atria exposed to low concentration of calcium or to verapamil.
2.A Report of the Seven Cases with Anomalous Origin of the Left Coronary Artery Abulling from the Pulmonary Arteries.
Liehu TANG ; Gengsheng ZHENG ;
Journal of Interventional Radiology 1994;0(03):-
During the period of 1960~1993,seven cases with anomalous origin of the left coronary artery arising from the pulmonary arteries were admitted to our hospital.Some of them were associated with other complex defects.One combined with mitral regurgitation and the other with patent ductus arterious.(PDA).The ratis of male to female was 2:5.The pa- tients age ranged from 5 to 37 years.(mean=24years).They uselly presented the symp- toms of palpitation,breathlessness,asthenia,vertigo,fatigue and angina;Echocardiography was valuable but selective angiocardiograpohy was the most useful diagnostic measure.Oper- ation of surgical bypass between aorta and left coronary artery with ligation of the fistula,as reconstruction of LCA were the major methords of choice is for the correction.Therapeutic effect of the operations were satisfactory in all cases.
3.Observation on the Myocardial Ceil Action Potential of 12 Snecies Experimental Animals in situ
Xiaoguang LI ; Dengcheng ZHANG ; Zheng MING ; Gengsheng CHAO
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(03):-
With flexibly mounted ultra microelectrode, we have succesfully recorded the myocardial cell action potentials from the following 12 species experimental animals in situ: dog, sheep, pig, cat, rabbit, guinea pig, rat, mouse, chicken, pigeon, toad and frog.
4.Application of VasoCT in stent-assisted coil aneurysm embolization
Xiaowang NIU ; Juan ZHENG ; Shiwei DU ; Qingbin NIE ; Manman GAN ; Dongmei LI ; Youping ZHANG ; Gengsheng MAO
Chinese Journal of Cerebrovascular Diseases 2015;(6):311-315
Objective ToexploretheapplicationofVasoCT,astentimagingtechnique,instent-assisted coilaneurysmembolization.Methods Twentyconsecutivepatientswith23intracranialaneurysmswere treated with stent-assisted aneurysm embolization in the General Hospital of Armed Police Forces from December 2013 to November 2014 were enrolled. The patients performed VasoCT scan immediately after procedure. Then all the available images were used for stent-optimized reconstruction respectively. Under the XpertCT mode,the available images were observed with both volume imaging and maximum intensity projection. The available images were analyzed and they were divided into very clear,less clear,and not clearaccordingtothestentdevelopingclarity.Results Ofthe22aneurysmstreatedwithstent-assisted embolization,16 were occluded completely,6 were occluded partially. All the stents were expanded completely and were released to the expected locations;11 aneurysm stents developed clearly,9 developed less clearly,and 2didnotdevelopclearly.Conclusion VasoCTcanbeusedinthestent-assistedaneurysmembolization.It can clearly reveal the microscopic structure of the stents,location,relationship with the artery wall,and relationship between stents and coils. The clarity of stent development is associated with the diameters of the packed coils,and the stents are also affected by the metal artifacts projecting direction and the dense degree of the packing coils.
5.Change of ACE2 level in serum during development of coronary heart disease
Juan CHEN ; Yubi LIN ; Gengsheng YIN ; Zicheng LI ; Wanqun CHEN ; Juan HU ; Linlin TAN ; Shaoling XU ; Dongling ZHENG ; Yongquan PAN
Chinese Journal of Pathophysiology 2017;33(6):1086-1090
AIM:To analyze the correlation between serum angiotensin-converting enzyme 2 (ACE2) levels and different stages of coronary heart disease (CHD), and to explore the change of serum ACE2 level during the development of CHD.METHODS:The control group included 85 non-CHD samples, and 174 CHD samples were divided into light stenosis (ls-CHD, stenosis degree <50%) group, moderate stenosis (ms-CHD, stenosis degree 50%~75%) group and severe stenosis (ss-CHD, stenosis degree ≥75%) group.The ACE2 level in each serum sample was detected by ELISA.The relationship between the ACE2 level and the development of coronary heart disease was explored by statistical analysis of serum ACE2 levels in different stages of CHD.RESULTS:The serum ACE2 levels in ls-CHD group, ms-CHD group and ss-CHD group were all higher than that in control group.The more severe the coronary artery stenosis existed, the higher the ACE2 level was observed.The serum ACE2 level in the males was higher than that in the females.In a single sex, the serum ACE2 levels in ls-CHD group, ms-CHD group and ss-CHD group were higher than that in control group with significant differences.Regression analysis found that sex, diabetes and CHD were associated with the serum ACE2 levels.Among them, sex and CHD were the independent factors to affect serum ACE2 levels.CONCLUSION:The serum ACE2 level of males was higher than that of females.Compared with the non-CHD samples, the serum ACE2 level of CHD patients was higher than that of the non-CHD samples.During the development of coronary heart disease, the serum ACE2 level increased constantly.
6. Quantitative structure-activity relationship prediction of carcinogenicity of N-nitroso compounds based on category approach and read-across
Qianqian LIANG ; Weiwei ZHENG ; Gengsheng HE ; Weidong QU
Chinese Journal of Preventive Medicine 2017;51(7):621-627
Objective:
New quantitative structure-activity relationship (QSAR) method was used to predict N-nitroso compounds (NOCs) carcinogenicity. This could provide evidences for health risk assessment of the chemicals.
Methods:
Total 74 chemical substances of NOCs were included as target chemicals for this validation study by using QSAR Toolbox based on category approach and read-across. The included 74 NOCs were categorized and subcategorized respectively using "Organic functional groups, Norbert Haider " profiler and "DNA binding by OASIS V.1.1" profiler. Carcinogenicity of rat were used as target of prediction, the carcinogenicity
results:
of analogues in chemical categories were cross-read to obtain the carcinogenic predictive results of the target chemicals. Results 74 NOCs included 26 nonclic N-nitrosamines, 24 cyclic N-nitrosamines and 24 N-nitrosamides The sensitivity, specificity and concordance of the category approach and read-across for predicting carcinogenicity of 74 NOCs were 75% (48/64), 70%(7/10) and 74% (55/74) respectively. The concordance for noncyclic N-nitrosamines, cyclic N-nitrosamines and N-nitrosamides were 88% (23/26), 71% (17/24) and 63% (15/24) respectively.
Conclusion
QSAR based on category approach and read-across is good for prediction of NOCs carcinogenicity, and can be used for high-throughput qualitative prediction of NOCs carcinogenicity.
7. Management and safety assessment of nanomaterials in food: status and prospects
Ziqi LI ; Weiwei ZHENG ; Ying LIU ; Ying ZHOU ; Gengsheng HE ; Yuxin ZHENG ; Weidong QU
Chinese Journal of Preventive Medicine 2018;52(10):1082-1088
The safety assessment of nanomaterials in food is essential for safeguarding supervision and maintaining public health. However, there are still no safety assessment procedures for nanomaterials established in national-level in China and no specific toxicology and safety assessment procedures about nanomaterials for food, too. These factors lead to restriction on food safety protection and supervision. Current methods of evaluating the safety of nanomaterials mainly rely on traditional toxicological assessment that are extrapolated based on animal experiment from high doses to low doses and from animals to humans. These uncertainties restrict the accuracy of safety assessment for nanomaterials and also limit the development of scientific and effective evaluation procedures and regulatory measures. Currently, the key issues need to be solved including exposure assessment and evaluation methods of nanomaterials in food and the established methods of the toxicity test for nanomaterials that are consistent with the objectives of toxicity test in the 21st century vision and strategy. In this article, we reviewed current administrative regulatory, situations, and existing issues of food nanomaterials either in China or some developed countries in order to provide a scientific basis in establishing safety assessment procedures for nanomaterials in food in the future.
8.Effect of Yunvjian with or Without Achyranthis Bidentatae Radix on Glucose and Lipid Metabolism and Inflammatory Response in Diabetic Rats with Syndrome of Yin Deficiency and Internal Heat
Ruifeng LIANG ; Wenjing GE ; Xianmei SONG ; Pengtao SHAN ; Gengsheng LI ; Zheng WEI ; Mingli ZHANG
Chinese Journal of Experimental Traditional Medical Formulae 2024;30(2):46-55
ObjectiveTo investigate the different effects of Yunvjian with or without Achyranthis Bidentatae Radix on glucose and lipid metabolism and inflammatory response in diabetic rats with the syndrome of Yin deficiency and internal heat. MethodThe rat model of diabetes due to Yin deficiency and internal heat was established by feeding with a high-sugar and high-fat diet and injection of thyroxine and streptozotocin. The successfully modeled rats were randomized into model control, Yunvjian without Achyranthis Bidentatae Radix (11.8 g·kg-1), Yunvjian with Achyranthis Bidentatae Radix (12.8 g·kg-1), and Achyranthis Bidentatae Radix (1.0 g·kg-1) groups (n=10), and another 10 rats were taken as the normal control group. Each group was administrated with corresponding drugs or saline by gavage for 28 days. The fasting blood glucose (FBG), fasting insulin (FINS), total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in rats were measured. Enzyme-linked immunosorbent assay was employed to determine the levels of cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), triiodothyronine (T3), thyroxine (T4), interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, and C-reactive protein (CRP) in the serum. The histopathological changes of the liver were observed. The expression of lipoxygenase-2 (COX-2) was detected by immunofluorescence. The mRNA levels of nuclear transcription factors-κB (NF-κB), monocyte chemotactic protein-1 (MCP-1), and intercellular adhesion molecule-1 (ICAM-1) were determined by real-time polymerase chain reaction (Real-time PCR).Western blot was employed to determine the protein levels of NF-κB in hibitory protein(IκB) kinase β (IKKβ), IκBα, and phosphorylated IκBα (p-IκBα) in the liver and the protein levels of NF-κB in the cytoplasm and nucleus. ResultCompared with the normal group, the model group showed elevated levels of FBG, FINS, insulin resistance index, TC, TG, LDL-C, cAMP, T3, T4, IL-1β, IL-6, TNF-α, and CRP, up-regulated mRNA levels of NF-κB, MCP-1, and ICAM-1, and up-regulated protein levels of COX-2, p-IκBα, and nuclear NF-κB (P<0.01). Compared with the model group, Yunvjian without Achyranthis Bidentatae Radix lowered the levels of FBG, FINS, insulin resistance index, TC, TG, LDL-C, cAMP, T3, T4, IL-1β, IL-6, TNF-α, and CRP, down-regulated the mRNA levels of NF-κB, MCP-1, and ICAM-1, and down-regulated the protein levels of COX-2, p-IκBα and nuclear NF-κB (P<0.05, P<0.01). Compared with the Yunvjian without Achyranthis Bidentatae Radix, Yunvjian with Achyranthis Bidentatae Radix showed lowered levels of FBG, FINS, insulin resistance index, and inflammatory cytokines, down-regulated mRNA levels of NF-κB, MCP-1, and ICAM-1, and down-regulated protein levels of p-IκBα and nuclear NF-κB (P<0.05, P<0.01). ConclusionAchyranthis Bidentatae Radix can enhance the performance of Yunvjian in reducing blood glucose and inhibiting inflammation in diabetic rats with the syndrome of yin deficiency and internal heat by down-regulating the IKK/IκB/NF-κB signaling pathway.
9. Research progress on exposure levels and toxic pathways of typical persistent organic pollutants in foods
Wuren MA ; Ying QING ; Ziqi LI ; Zhiyuan CHEN ; Yu HUANG ; Wei LU ; Lan YANG ; Weiwei ZHENG ; Wen CHEN ; Yuxin ZHENG ; Jia CAO ; Gengsheng HE ; Weidong QU
Chinese Journal of Preventive Medicine 2019;53(6):645-652
Dioxins, polybrominated diphenyl ethers, and benzo(a)pyrene are common organic pollutants in food. They have been of concern to academics and government administrations due to high residue and persistence, easy accumulation and strong harmful effects. The National Research Council of the United States of America published