Objective To observe the time profile of the changes of mRNA of ? and ? subunit of acetylcholine receptor (AChR) in skeletal muscle and the level of calcitonin gene related peptide (CGRP) in spinal motoneuron after upper motoneuron injury. Method The spinal cord of adult male Wistar rats was transected on the lower thoracic segment. The spinal cords below the transection level and soleus muscles were sampled 0,1,2,4,7 and 14 days after surgery, respectively, to detect the levels of mRNA of ? and ? subunit of acetylcholine receptor in soleus muscle using Northern blot method,and CGRP level in spinal motoneuron using radioimmunoassay.Results The levels of mRNA of ? and ? subunit of acetylcholine receptor siginificantly increased after surgery, but the level of mRNA of ? subunit increased earlier and higher than ? subunit. The level of CGRP in spinal motoneuron markedly reduced after surgery, was maintained at 50 80% of normal level since the 2nd day after surgery. Conclusions The levels of AChR ? and ? subunit in skeletal muscle significantly increase after upper motoneuron injury, the expression of which CGRP may regulate.

Objective To assess the changes in coagulation induced by acute normovolemic hemodilution Methods Twenty ASA Ⅰ Ⅱ patients scheduled for elective surgery were divided into two groups: HES group and GEL group in which hydroxyethyl starch(HES) or succinylated gelatin (GEL) was used as the hemodilution fluid Patients with coagulation abnormalities or kidney dysfunction were excluded from the study Anesthesia was induced with propofol,fentanyl and vecuronium and maintained with inhalation of isoflurane and nitrous oxide and intermittent intravenous boluses of fentanyl and vecuronium The patients were mechanically ventilated and PaO 2 and PaCO 2 were maintained within normal range After induction blood was withdrawn from internal jugular vein and collected in standard blood bags containing anticoagulant and at the same time replaced with HES (equal volume) or GEL (1 5 times the volume of blood withdrawn) The amount of blood withdrawn was estimated from the following equation:blood volume withdrawn=body weight(kg)?60?(Hct 0 Hct 1)/Hct M,where Hct 0=patient′s initial Hct, Hct 1=desired end point Hct and Hct M=the average of Hct 0 and Hct 1 Blood routine,prothrombin time(PT),activated partial thromboplastin time(APTT) and fibrinogen level were checked and thromboelastography(TEG) was performed 15 min before and after hemodilution Results The amount of blood withdrawn ranged between 1000 1100ml Hb was still above 80g/L after hemodilution The decreases in Hb and Hct were significant in both groups (P

OBJECTIVE:To observe the efficacy of Radix Astragali Injection in combination of Kallidinogenase for diabetic nephropathy.METHODS:A total of 78 patients with diabetic nephropathy were enrolled and randomly assigned to receive Kallidinogenase plus Radix Astragali Injection orally(trial group)or Kallidinogenase alone(control group)orally for 4wk.The main outcome measures were UPE,Alb,?2-MG,THP,Scr,BUN etc.RESULTS:After treatment,all the indexes were decreased significantly(P

Whole-body PET-CT scanning has been used in the diagnosis and treatment of tumor to providing functional and morphological imaging. As the most widely used semi-quantitative analysis index, the cutoff value of SUV would affect the final diagnosis, therapeutic regimen and prognosis estimation. Therefore, the article reviews the influential factors of SUV, its correction methods, its cutoff values of differential various tumors and the progress of SUV. Although there is no acknowledged cutoff value of SUV in tumor diagnosis, SUV is still an important semi-quantitative index, and its rational and prudent use would increase both of the sensitivity and specificity in tumor diagnosis.

PET-CT, SPECT, and SPECT-CT examinations could lead to inevitable medical exposure for patients, and the radiation dose of this examinations and whether it was harmful to patients have attracted attention from the public and professionals. At the same time, there was still a controversy about whether the biological effect of low dose radiation was useful to human body. The research investigates and reviews the radiation dose of PET-CT, SPECT, and SPECT-CT scans and their biological effects. Under achieved to the demands of clinical conditions, operators should try to reduce the radiation dose of PET-CT, SPECT, SPECT-CT scans for patients. Although PET-CT, SPECT, SPECT-CT scans deliver inevitable radiation, the dose of single examination will not lead to deterministic effect, and even the view of the random effect of linear no-threshold from these scans also have been questioned.

Objective To compare the clinical effect of two ways of the closed reduction internal fixation and open reduction internal fixation in the treatment of joint injury.Methods According to the digital table,84 patients with joint injury were randomly divided into control group and treatment group,42 cases in each group.The control group was given closed reduction and internal fixation,and the treatment group was given open reduction and internal fixation.Results The total time of operation,postoperative hospitalization time,postoperative joint function recovery time of the treatment group were (78.51 ±10.20)min,(13.35 ±2.46)d,(88.43 ±5.27)d,those of the control group were (118.73 ±25.71)min,(19.72 ±3.57)d,(107.42 ±10.33)d,the differences were statistically signifi-cant(t =9.424,9.522,10.613;P =0.000,0.000,0.000).The excellent and good rate of the treatment group was 90.48%,which was higher than 71.43% of the control group,the difference was statistically significant(χ2 =4.941, P =0.026).The treatment group had 1 case of adverse reaction,the control group had 8 cases of adverse reaction, there was statistically significant difference between the two groups(χ2 =6.098,P =0.014).After treatment,the VAS pain score in the treatment group was (2.11 ±0.68)points,which was lower than (4.98 ±1.35)points in the control group,the difference was statistically significant(t =12.305,P =0.000).Conclusion The application effect of closed reduction and internal fixation for patients with joint injury disease is very obvious.

Bacterial Infections ; drug therapy ; metabolism ; Biomarkers ; analysis ; Clinical Trials as Topic ; Humans ; Infant, Newborn ; Neonatology ; Research Report

Adult ; Cardiac Catheterization ; adverse effects ; Female ; Heart Septal Defects, Atrial ; therapy ; Hemolysis ; Humans

AIM : To prepare a phase-specific drug delivery system withfloating and pulsatile release of sinome-nine hydrochloride and evaluate in vitro drug release behavior. METHODS: The floating and pulsatile-release of coat-core tablets were prepared by press-coated technics. The effects of factors influencing release characteristic of the drug were investigated by dissolution test, and to elucidate the mechanism of drug releaseof the tablets with erosion and water-uptake test. RESULTS: The tablets had typical floating and pulsatile release properties with a lag time rapid release. The lag-time was shortened with the increase of expansion ratio of tablet core and rotation speed of stirrer. The lag-time was prolonged with the increase of pH and ionic strength of dissolution media.CONCLUSION: The tablet could float and rapidly release drug at the predetermined time.

Objective To observe the morphological changes of mice cortex neuron cultured in vitro under different temperature, and the expression of skeleton protein (?-tubulin) in the neuron, and to study the relationship between ?-tubulin and heat shock protein 70 (HSP70). Methods The cerebral cortex neuron of embryonic mice was cultured in vitro. The cultured neuron was put in different temperature 7 days later. To observe the morphological changes of the neuron using optical microscope and the changes of the expression of ?-tubulin and HSP70 under different temperature using laser scanning confocal images. Results Optical microscopy indicated that drifting cells increased, and neural network became sparse in 38℃; some cells necrosed in 39℃; most cells necrosed, cell broke to pieces, axons drifted or disappeared in 42℃. Results of laser scanning confocal images indicated that after hyperthermia the fluorescence intensity of ?-tubulin was lower than that of controls, and the fluorescence intensity declined as the temperature elevated. The fluorescence intensity of HSP70 showed a bell-shape distribution curve, i.e. the highest value emerged at 39℃, whereas the lower values appeared at 37℃ and 42℃. Conclusion Heat stress leaded to the morphological changes of neuron. The disordered of skeleton protein may be responsible for the changes and HSP70 may take part in the process.