The study of blood samples extracted directly from healthy people, who is Vietnamese and not relative, lives over the country. PCR technique and denaturing polyacrylamid gel electrophoresis were applied for determination of allele frequencies of D13S317. The primary results showed that allele frequencies of D13S317 locus was polymorphic AND, repetitive sequence unit of 4 nito- base which located on the long hand of chromosome No 13. The locus contained 9 alleles, 242-270 bp. The numbers of repeat units in population were 7, 8, 9, 10, 11, 12, 13, 14, 15. The highest frequency at allele No 8, and allele No 7 had not appear in the observed samples.
Population ; Gene Frequency ; analysis

Alleles ; Gene Frequency ; Genotype

Experimental study for extracting DNA and determining HLA of I and II class by PCR-SSO was carried out. The percentage of allele frequency was estimated in comparison with that was reported in 1991 conference. Result showed no difference in locus A and DR12. Locus A demonstrated the highest percentage with some differences of 2-4%. In Locus DR12 with the highest percentage of differences of 3%. Highest difference was estimated in locus B. In this study, B15 has 25.7% (in comparing with 0%). B62 had 0% (in comparing with 10.1%), B75 6.7% (in comparing with 0%), B7 12.2% (in comparing with 0%)
DNA ; HLA Antigens ; Gene Frequency

No abstract available.
Alleles* ; AMP Deaminase ; Gene Frequency

Most common diseases are complex genetic traits. Identifying the genes that underlie the variation in the diseases has been challenging. Two major approaches have been used to map genetic variants that influence disease risk: linkage analysis and association study. Linkage analysis tests for the co-segregation of a marker and disease phenotype within a pedigree, whereas association study tests for differences in marker allele frequencies between patients and a control population. Linkage analysis is applied without any prior knowledge of the biological basis of the disease. In association study, genes with a known function with the potential to influence the disease phenotype are investigated for a direct role in disease. No single method is sufficient. A multi-strategy approach to the mapping of complex diseases is required. We review the different types of genetic studies to find genes for complex genetic traits.
Gene Frequency ; Humans ; Pedigree ; Phenotype

To determine allele frequency of GABARB1 Jcus in Korean, first, we sequenced each allele, which was amplified with primers that published before. We found mismatching between allele number and size of each allele when 'GATA' repeats are counted. The sequence revealed new 'GATA' repeat. So we had made new allele designation with total number of in 176 Korean people. The heterozygosity this locus was 0.716 and mean exclusion chance (MEC) and power of discrimination (PD) were 0.433 and 0.845, respectively. In this study, we emphasized necessity of sequencing of 2-3 alleles, which have same size before setting up allelic ladder.
Alleles* ; Discrimination (Psychology) ; Gene Frequency*

No abstract available.
Erythroblastosis, Fetal* ; Gene Frequency* ; Infant, Newborn

Research to build database of Nineplexll allelic frequency of Vietnam population applied for gene forensic purposes. The results as followed: there were 6 alleles in D3S1358; 19 alleles in FGA. In Viet (Kinh) population, polymorphism of vWA, especially of FGA was higher than in some other foreign population. For examples, the number of vWA alleles in Viet population is 9, but in Japanese and Caucasian population is 8; FGA alleles in Viet population is 19, but in Japanese is 15, and in Caucasian is 12. Two alleles of FGA are 14 and 32 were found out. There are many ethnics living in Vietnam, so the authors would like to survey allelic frequency of other ethnics.
Population ; Gene Frequency ; analysis ; Forensic Medicine

BACKGROUND: The FUT2 and FUT3 genes determine the Lewis phenotype of red blood cells (RBCs). Recently, the Lewis genes, the secretor genes, and several mutations that cause Lewis negative and nonsecretor phenotypes have been identified. The purpose of this study was to analyze the gene frequency of FUT2 and FUT3 in a Korean population by comparing the use of the direct sequencing method to the use of the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for mutation detection in the FUT2 and FUT3 genes. METHODS: RBCs and peripheral blood leukocytes were obtained from 225 apparently healthy volunteers to determine the phenotype and genotype of the FUT2 and FUT3 genes. Lewis phenotypes were determined on K3EDTA-stablized fresh blood samples using the column agglutination method. Lewis blood group genotyping was performed by use of the direct sequencing method. For the detection of T59G, C357T, and A385T mutations, the PCR-RFLP method was performed. RESULTS: The frequencies of the Lewis blood group phenotype were 12.4% for Le(a+b-), 70.7% for Le(a-b+), 11.1% for Le(a-b-) and 5.8% for Le(a+b+), respectively. Direct Sequencing of the FUT2 gene identified 92.2% C357T, 56.9% A385T, 0.4% G244A mutations and 1.8% del396. Direct Sequencing of the FUT3 gene identified 46.9% T59G, 30.4% G508A, 1.1% T202C, 1.1% C314T, 0.7% A1029G, 3.0% T1067A and 13.3% G1242A mutations. The PCR-RFLP method results were discordant in nine cases (1 case for C357T, 4 cases for A385T and 2 cases for T59G) as compared to the direct sequencing method results. CONCLUSION: We have determined the frequencies of FUT2 and FUT3 gene mutations in a Korean population. The use of the direct sequencing method was more accurate than the use of the PCR-RFLP method for the determination of the genotype of the FUT2 and FUT3 genes.
Agglutination ; Erythrocytes ; Gene Frequency ; Genotype ; Leukocytes ; Phenotype

Humans ; Siblings ; Gene Frequency ; Microsatellite Repeats