Objective:This study was to investigate the interrelations between the acetylase activity and specialization rate of the differentiation of MSCs to the cardiac cells when histon deacetylase enzyme inhibitor trichostatin A (TSA)interferes in the cardiac cells. Method: MSCs of ratswere separated,The already labeled MSCs and cardiac muscle cells were put respectively into the MSCs interfered by different concentrations of TSA cultivated in mix tune with cardiac muscles. Adding TSA into the conventional culture of MSCs for the negative group.One week later,with the help of immunofluorescence,detecting the expression of the function proteinum,ie. myosin heavy chain (MHC)and Connexin43 (Cx43),of the cardiac muscle constitution in MSCs was detected. Results: ① There appeared sample differentiation of the cardiac muscle cells after the coculture of the MSCs and the pulsating cardiac muscle cells for one week,and the expression of the function proteinum of the cardiac muscle constitution;② In the positive group,in the mix culture of the cardiac muscle cells and the MSCs in which the TSA interfered for 48 h,there appeard sample differentiation of the cardiac muscle cells;③ In the negative group,part of the MSCs could express the function proteinum of the cardiac muscle constitution. During the 7 days of coculture, with the induction of TSA,MSCs was double-labeled by Brdu-DAPI. The differentiation rate of the fluorescent manifestation of the MHC and Cx43 in the MSCs was notably different expression that in the positive group,Conclusion: MSCs in which HDAC,the enzyme inhibitor,interferes are in the microenvironment of the cardiac muscle cells. HDAC,the enzyme inhibitor,functions as a promotion factor in the specialization of MSCs,suggesting the interrelation between the acetylase activity and the cell specialization rates.

BACKGROUND:Piperine in models of pancreatitis, gout, middle cerebral artery infarction has anti-inflammatory, antioxidant and immune regulatory effects, but its effects on periodontitis model are not clear.
OBJECTIVE:To observe the protective effect of piperine on bone absorption and degradation of col agen in experimental rat models of periodontitis.
METHODS:Rat models of periodontitis were established by ligaturing the dental cervix of rat mandibular first molar with 3-0 silk. On day 1 before model establishment, rats were intragastrical y administered piperine 50 and 100 mg/kg. There were healthy control group and model group. Related detection was performed 8 weeks after model establishment.
RESULTS AND CONCLUSION:(1) Results of quantitative CT analysis:compared with the healthy control group, the distance from the first molar enamelo-cemental junction to the alveolar ridge crest was significantly lower in the model group (P<0.05). The degree of alveolar damage was significantly improved in the 50 and 100 mg/kg piperine groups compared with the model group (P<0.05). (2) Related factor protein expression:compared with the model group, matrix metal oproteinase-8,-13 and interleukin-1βprotein expression was significantly decreased in the 100 mg/kg piperine group (P<0.05);matrix metal oproteinase-8 protein expression was significantly decreased in the 50 mg/kg piperine group (P<0.05). (3) Col agen fiber morphology:compared with the model group, col agen fibers arranged orderly and col agen fiber area significantly increased in the 50 and 100 mg/kg piperine groups (P<0.05). (4) Results confirmed that piperine could reduce the alveolar bone resorption, reduce the degradation of col agen fibers and protect the periodontal tissues in models of periodontitis. Its mechanism is associated with the inhibition of matrix metal oproteinase-8,-13 and interleukin-1βprotein expression.

Objective To investigate the improvement role of low-dose aspirin combined with metoprolol in high blood coagulation and cardiac function of elderly patients with heart failure. Methods 112 cases of elderly patients with heart failure who were treated in our hospital from February 2014 to February 2016 were selected, these patients were randomly divided into conventional therapy group and combined treatment group (conventional therapy combined with low-dose aspirin beauty metoprolol treatment group) two groups, 56 cases in each group. Conventional therapy group was given routine treatment, combined treatment group wered treated on the basis of conventional therapy combined with metoprolol in the treatment of low-dose of aspirin. The plasma P-selectin, VWF, DD, BNP levels, HR, LVEDV, LVEF and clinical efficacy of the two groups were statistically analyzed. Results The P-selectin, VWF, DD, BNP levels of the combined treatment group were significantly lower than those in the conventional treatment group, the difference was statistically significant (P<0.05), the total treatment effective rate 89.3% (50/56) was significantly higher than the conventional therapy group 78.6% (44/56), the difference was statistically significant (P<0.05), but there was no significant difference between the two groups in HR, LVEDV, and LVEF. Conclusion Low-dose aspirin combined with metoprolol can improve the high blood coagulation and cardiac function of elderly patients with heart failure.

A series of activities on popularization of health sciences including nutrition and food safety, healthy lifestyle promotion, prevention and control of major infectious diseases, and decipher-ing the mystery of life have been carried out by Center for Laboratory teaching and management of Chongqing Medical University, using quality resources of teaching and scientific research, in order to explore the effective mode and long lasting mechanisms of work in popularization of sciences in higher education institutions. A combined method of quantification and qualification has been applied to sci-entifically evaluate the effect of the activities, and the results indicated that huge potentials and adva-ntages of higher education institutions existed in the field of popularization of sciences through coherent management system of the institution, as well as arousing the enthusiasm of teachers and students to take part in the activities.

Objective To investigate the anti-proliferation effect of Ginseng Rh2(G-Rh2)on mouse MFC gastric cells and its mechanism.Methods MFC cells (1.0?109?L-1) were divided into four gourps:control group and G-Rh2(3,10,30 mg?L-1) groups.The cell viability was determined by MTT;the cell cycle was detected by flow cytometry;the protein expression of cyclin D1 and p27kip1 were observed respectively by qualitative and quantitative analysis.Results Compared with control group,the cell viabilities decreased gradually with the increasing of dose and time in G-Rh2(3,10,30 mg?L-1) groups at different time(1,2,4 h)(P

Aneuploidy ; DNA, Neoplasm ; analysis ; genetics ; Flow Cytometry ; methods ; Humans ; Lymphoma ; diagnosis ; genetics ; immunology ; Lymphoma, B-Cell ; diagnosis ; genetics ; immunology ; Lymphoma, T-Cell ; diagnosis ; genetics ; immunology ; Receptors, Antigen, T-Cell ; analysis ; genetics

In vitro fertilization and embryo transfer (IVF- ET) technology is now widely used in the world in infertility patients. Psychological intervention can help the IVF- ET patients and their spouses reduce the negative emotions and improve pregnancy outcomes. This review provides a summary of the negative emotions in IVF- ET couples and the psychological intervention on them, so as to provide reference to establish a systematic and standardized clinical psychological nursing mode.

Objective To monitor the release of amino acids of the whole retina during and after experimental glaucoma by increasing the intraocular pressure (IOP). Methods Experimental glaucoma was induced in one of the two eyes of rabbits by increasing IOP at 120 mm Hg for 45 min under infusion of saline in anterior chamber;then the pressure was released and the needle inserted into the anterior chamber was removed,this state was maintained for another 45 min.Every 15 min during the experiment 5 rabbits were killed and experimental eyes were enucleated.Aliquots (20 ?l) of the retinal extracts (see below) were mixed with ophthaldialdehyde reagent and analysed for amino acid content by the HPLC method of Wangwei, using a 150 mm?4.6 mm,5 ?m C18 column. Results A large increase in the release of glutamate,but not of the other three amino acids monitored,occurred during initial experimental ocular hypertension.It reached peak value of (111.73?17.46) 10 -5 mmol/g at 15 min of hypertension.15 min after release of intraocular pressure,again,immediately large and specific increase in the concentration of glutamate was reached to (102.96?51.91) 10 -5 mmol/g.In eyes subjected to paracentesis of anterior chamber,no difference was found between experimental eyes and controls. Conclusion These results suggest that glutamate is triggered by increasing the IOP,and it releases not only during the period of experimental ocular hypertension,but also afterwards.

Objective To investigate the possibility of commitment differentiation of embryonic stem cells induced by the medium of cultured retinal neurons of SD rats. Methods The medium from cultured retinal neurons of SD rats were collected, sterilized and mixed with DMEM medium according to 2∶3 proportion, ES cells were cultured with these mixed medium and were observed under the phase contrast microscope daily, the induced cells were identified by NF immunohistochemistry methods. Results The ES cells cultured with these mixed medium can differentiate into neuron like structure, and the induced cells were positive in NF immunofluorescence staining. Conclusion The medium from cultured retinal neurons of SD rats can induce ES cells commitment differentiation into neuron like structure.

Objective To explore an efficient treatment for bladder outlet obstruction caused by small benign prostatic hyperplasia(BPH). Methods From January 1996 to January 2005,69 patients with BPH were surgically treated.Of these patients,27 cases underwent open surgery,22 transurethral resection of prostate(TURP) and 20 TURP plus transurethral incision of bladder neck(TUIBN).There were no significant differences between the 3 groups in age,disease course,preoperative prostate weight and the excised prostate weight.The efficacy of open surgery,TRUP and TURP plus TUIBN were comparatively evaluated by international prostate symptom score(IPSS),peak urinary flow rate(Qmax),post void residual drine volume(PVR) and postoperative complications.Results After operation,the mean score of IPSS of patients which treated with open surgery decreased from(24.6+3.8) to(15.1?3.8),and the Qmax increased from(8.2?3.1)ml/s to(10.5?4.2)ml/s,and the PVR decreased from(96.0?36.0)ml to(54.0?27.0)ml.The IPSS of TURP group decreased from(22.3?5.6) to(11.7?2.7),and the Qmax increased from(8.5?3.6)ml/s to(11.4?4.2)ml/s,and the PVR decreased from(105.0?39.0)ml/s to(32.0?14.0)ml/s.The IPSS of the TURP plus TUIBN group decreased from(23.6?5.7) to(6.4?2.3),and the Qmax increased from(9.1?3.8)ml/s to(19.5?6.2) ml/s,and the PVR decreased from(98.0?37.0)ml to(8.0?5.0)ml.There were significant differences between the TURP plus TUIBN group and the other two groups in the IPSS,Qmax and PVR(P