Matrix metalloproteinases (MMP) are a family of proteolytic enzymes that degrade various components of the extracellular matrix (ECM).Both animal expodments and clinical sample analysis have shown that balance in expression and activation of MMP and inhibition by tissue inhibitors of matrix metallo-proteinase (TIMP)is critical for the development of stenotic change.This review article focuses on the role of MMP relevant to restenosis after vascular injury.

Objective To compare the diagnostic values of different parameters for neonatal sepsis by observing clinical presentations and laboratory tests of hospitalized newborn infants and to find out the the early and fast way in neonatal sepsis diagnosis. Methods Newborn infants admitted to NICU from Sept.2007 to Feb.2008,who met all inclusion and exclusion criteria,were enrolled.The situations in perinatal period,exact time of the onset of infection,clinical manifestations and laboratory indexes were recorded.Infants were classified into 3 groups:septic group(n=13),nonseptic group(n=12) and noninfectious group(n=12).Sensitivity,specificity,false positive rate,false negative rate,Youden's index and positive and negative predictive values(PPV and NPV) were calculated for each test. Receiver-operating characteristic curves were analyzed to determine the optimal thresholds. Results Among all the clinical manifestations,abnormal reaction had the hightest specificity(84.6%) and accuracy(67.9%) in diagnosing neonatal sepsis.SIRS criteria,with the specificity of 95.8%,was helpful in clinical suspected eases.However,routine laboratory tests,such as WBC,PLT and GLU,had poor sensitivity or specificity;I/T had better specificity(100%),but was subject to discrepancy among different observers.The area under the ROC for CRP and PCT were 0.734 and 0.878,with the optimal threshold of 15 mg/L(P=0.020,Youden's index=45.5%) and 0.81 ng/ml(P-0.000,specificity=100%,Youden's index=75.0%),respectively.Conclusions Careful clinical inspection warrants early detection of sick infants.Evaluation of SIRS criteria in clinical suspected septic infants may help in early diagnosis.WBC,I/T,PLT can be used as routinely monitoring indexes for high risk neonates.CRP and PCT concentrations also have significant diagnostic values.

Objective To investigate the diagnosis method of intervertebral disc low back pain and the best treatment clinical efficacy. Methods The imaging data of 132 cases with intervertebral disc low back pain diagnosed by discography for disc imaging was retrospectively analyzed from October 2001 to October 2008. Randomly divided into three groups: group A of pereutaneous laser disc decompression in 63 cases of gasification, group B extraperitoneal anterior disectomy, Cage fusion 21 cases, group C posterior fusion after disectomy arch root 48 cases of screw fixation. Observed in patients with postoperative visual analogue scale (VAS) and Oswestry disability index (ODI) score improvement. Results MRI of lumbar intervertebral disc on T_2-weightod lesions as low signal was important. VAS after treatment in three groups followed by lower values to reduce, group A was (9.7 ± 0.9) points, group B was (7.4 ± 1.2) points, group C was (6.9 ± 1.4) points. There were statistically significance between group A and group B, C (P< 0.05). ODI in order to reduce the value of reducing, group A was (19.4 ± 1.4) points, group B was (18.8 ± 2.9) points, group C was (16.5 ± 2.1) points. There were statistically significance between group A and group B, C (P< 0.05). Conclusions Discography is a reliable method to diagnose intervertebral disc low back pain. Percutaneens laser disc decompression compared with posterior or anterior disectomy and fusion combined with internal fixation has good results in improving pain and function of patients.

Objective:To investigate the effects of soluble factors secreted by MCF-7 breast cancer cells on the differentiation,maturation and function of DCs.Methods:Mononuclear cells were cultured with the culture supernatants from primary MCF-7 cells.Combination of granulocyte-macrophage colony stimulating factor(GM-CSF),interleukin 4(IL-4) and tumor necrosis factor-a(TNF-a) was used to stimulate the cultured mononuclear cells.Then DCs and CTL assays were analyzed.Results:MCF-7 cell supernatant-treated DCs resulted in low expression of CD80,CD83,CD86 and HLA-DR,and the inhibition rate of CTL was 17.35% significantly lower than 56.14% induced by the control DCs(P＜0.01).IL-12 secreted by DCs and IFN-γ produced by PBMNC were all significantly lower than those in the control group (P＜0.01).Conclusion:Experiments in vitro shows that the culture supernatants from MCF-7 breast cancer cells could inhibit the development and functions of DCs.

It has been fully studied about interstitial cells of Cajal in gastrointestinal tract,and in recent years these cells are discovered in other tissues and organs,such as urinary system,cardiovascular system,reproductive system,liver,pancreas and mammary gland.The criterion and process reported by some authors are reliable for invesgating these cells,and can be helped to study the distribution and function of these cells outside gastrointestinal tract.It is showed that the fuction of interstitial cells of Cajal exsited in urinary organs seems to be related with the spontaneous rhythmic contraction of smooth muscle,while in heart seems to cause atrial fibrillation in humans,but the poteinal fuctions of interstitial cells of Cajal in other tissues and organs have not been revealed.

Objective To study the clinical characteristics and the effect of enzyme replacement therapy for late-onset glycogen storage disease typeⅡ(GSDⅡ). Methods The clinical, laboratory data and the result of genetic testing were retrospectively analyzed in a GSDⅡchild, the effect of enzyme replacement therapy was followed up and the relevant literature was reviewed. Results The patient had motor regression after 1 year old, the serum creatine kinase level is from 675 to 1286 U/L. The EMG test showed myopathic change, acid alpha-glucosidase activity is 12.0 nmol/(g·min), next generation sequencing of genetic muscle diseases panel found the GAA compound heterozygous mutations, both were tiny variations, and muscle biopsies showed the typical pathological features of GSD. The patient was given human recombinant of alpha glucoside enzyme 20 mg per kilogram of body weight, once every other week for 1 year. The weakness of the patient’s muscle strength had no obvious aggravation. Conclusions Early and adequate enzyme replacement therapy is the only possible treatment for GSDⅡ.

Objective To investigate the relationship between fatty liver prevalence and body mass index(BMI),dyslipidemia in physical examination population in Baoji area.Methods The height and weight of 10 476 cases of physical examination population were measured and then BMI was calculated.Serum TG,TC,LDL-C and HDL-C concentrations were also determined.Type-B ul-trasonic was used to diagnose fatty liver.The test results were analyzed statistically.Results Fatty liver detection rate of 10 476 physical examination people was 16.29%,the detection rate of abnormal blood lipid concentration was 45.31%;the detection rate of abnormal TG,TC,LDL-C and HDL-C concentrations were 33.05%,17.23%,15.48% and 10%;the prevalence of fatty liver in-creased with BMI(P <0.05);In male population,the detection rate of TG,LDL-C concentrations′abnormal increase,HDL-C con-centrations′abnormal decrease,and fatty liver were higher than that in female population(P <0.05);the detection rate of blood lip-id′s abnormal concentrations and fatty liver were different in people of different age(P <0.05).In male population the group with the highest detection rate of dyslipidemia and fatty liver was the middle-aged group,while in women population was the elderly group.Conclusion There was a significant correlation among BMI,dyslipidemia and fatty liver prevalence,which were different in age and gender.

The Non-neuronal Acetylcholine System,including acetylcholine(NNAs),choline acetyltransferase,acetylcholinesterase,muscarinic and nicotinic ACh receptors,has been identified in numerous non-neuronal cells and tissues,including keratinocytes,cancer cells,immune cells and reproductive organs.NNAs is involved in regulation of their function and related to the pathophysiology of several diseases.This review investigates the research progress of NNAs and describes the structure and function of NNAs in different cells,such as glial cell,endothelium,epithelia and lymphocyte.NNAs is not the same as neuronal acetylcholine system,neither is the same in different cells.

Objective To explore the inhibitory effects of RNAi targeting survivin to suppress the expression of survivin and to induce the apoptosis of human renal cancer cell line 786-O.Methods The target gene fragments were cloned into pcDNATM 6.2-GW/EmGFPmiR vectors,and then the recombinant eukaryotic expression vectors were transfected into 786-O cells with liposomes.The expression changes in survivin mRNA and protein were detected by RT-PCR and immunohistochemical assay,and the proliferation inhibitory rate (IR) of 786-O cells was detected by M'TT assay.Results The mRNA and protein expressions of survivin in the siRNA group were significantly inhibited by the eukaryotic expression vectors,and it also inhibited significantly the growth and proliferation of 786-O cells.Conclusion Silencing survivin gene by the RNAi technique can not only effectively decrease the expressions of survivin gene and protein,but also inhibit the cell proliferation in 786-O cells.This may provide the experimental evidence for the survivin gene as a target of gene therapy in renal cancer.

Objective To explore the suitable protocols of thoracotomy inhalation program for newborns, which will help to promote respiratory tract secretion drainage and to prevent the postoperative pulmonary complications.Methods This was a double-blind,randomized clinical trial.From January 2011 to October 2013 ,a total of 60 neonatal cases received thoracotomy were recruited,which included 45 cases of congenital esophageal atresia, and 15 cases of congenital diaphragmatic hernia.All cases were randomly allocated into treatment group and control group.Treatment group received mucosolvan 7.5 mg and saline 2 ml oxygen driven inhalation and the control group was given dexamethasone 1 mg, α-chymotrypsin 1 000 U plus saline 10 ml ultrasonic atomizing inhalation.The postoperative incidence of blood oxygen falling when receiving atomizing inhalation and numbers of sputum suction were compared between two groups.Results Compared with control group, the incidences of drop of blood oxygen were significantly deceased [the first day:(0.40 ± 0.35) times vs.(1.53 ± 1.04) times;the second day: (4.32 ± 0.40) times vs.(1.10 ± 1.06) times;the third day: (0.51 ± 0.39) times vs.(1.20 ± 1.09) times;P ＜ 0.05], and the numbers of artificial sputum suction significantly deceased within 3 days after operation [the first day: (7.37 ± 4.92) times vs.(13.24 ± 5.81)times;the second day: (6.34 ± 3.84) times vs.(11.27 ± 4.64) times;the third day: (6.01 ± 2.23) times vs.(10.20 ± 4.53) times;P ＜ 0.05].No adverse reactions were found during treatment.The incidence of blood oxygen falling and the numbers of artificial sputum suction showed no significant differences between two groups on day 4 and day 5 after operation(P ＞ 0.05).Conclusion It is important to have mucosolvan inhalation program for improving condition of respiration and respiratory secretions drainage for newborns with thoracotomy mucosolvan oxygen driven inhalation is worthy being used widely.