1.Hijacking The Eukaryotic MAPK Pathway by Pathogens
Progress in Biochemistry and Biophysics 2006;0(09):-
MAPK(mitogen-activated protein kinase) family,which is conserved throughout high eukaryotes,is implicated in multiple cellular processes including cell growth,migration,proliferation,differentiation,survival and development.Pathogen hijacks hosts' MAPK pathways to facilitate its pathogenesis using diverse strategies.To further explore the mechanism underlying interactions between pathogens and hosts' MAPK pathway,is of benefit to our understanding of nature as well as to our fight against pathogen infection.
2.A genetic transformation study of introducing wild Gastrodia elata DNA into Solanum tuberosum by soaking seedling method
Zhenglong GE ; Hefeng ZHOU ; Min SHAO
Chinese Traditional and Herbal Drugs 1994;0(07):-
Objective Using the soaking seedling method to introduce wild Gastrodia elata DNA into potato plantlets and analyze gastrodin of transformed Solanum tuberosum.Methods After the tuber grown up,the solution of gastrodin was extracted from the potatoes which were transformed by wild G.(elata) DNA.The transformed plants were scaned by ultraviolet.PCR was used to analyze GAFP gene by SDS-PAGE.TLC was used to analyze the gastrodin of transformed S.tuberosum.Results(1)The 21 transformed plants had obvious absorption peak at 220 nm in 200 transformed S.tuberosunr.(2)The fragment of wild G.elata GAFP gene was found by PCR from 5 transformed plants.(3)The protein expressions in transgenic S.tuberosum were obviously different from the normal S.tuberosum;a band was detected in transgenic S.tuberosum and wild G.elata,but wasn't detected in normal S.tuberosum.(4)The expression of the gastrodin was detected by TLC from three transformed plants. Conclusion It is feasible to use the soaking seedling method to introduce exogenous DNA into plants.
3.Curative Effect Observation of Ginkgo Biloba Extract on Back Chapped Skin Model in Rats
Ge SHAO ; Ying CHEN ; Mingjie PAN
China Pharmacist 2016;19(4):656-659
Objective:To determine the curative effect of Ginkgo Biloba extract on the back chapped skin in SD rats. Methods:Totally 60 SD rats were randomly divided into 6 groups with 10 rats in each. One group was the blank control group, while the other five groups with the same back chapped skin were respectively treated with different therapy regimen, namely one group was the model group without any treatment, one group was urea group treated with the commercially available urea, and the last three groups were re-spectively treated with Ginkgo Biloba extract at the dose of 5%, 1% and 0. 2%. The pathological tissue was observed after HE stai-ning, and the content of hydroxyproline ( Hyp) in skin was determined by alkaline hydrolysis. Results:The skin lesion and the score of pathological section were studied and the content of Hyp in the damaged skin was determined. The comprehensive score for the groups was 0, 13. 2, 7. 4, 11. 0, 5. 8 and 6. 6, respectively, and the content of Hyp was (4. 57 ± 0. 07), (2. 41 ± 0. 27), (3. 29 ± 0.57), (2.55 ±0.31), (3.24 ±0.26) and (3.37 ±0.24) μg·mg-1,respectively. There was significant difference in the curative effect between the Ginkgo Biloba extract groups at high dose (5%) and medium dose (1%) and the model group (P<0. 05), which showed no obvious difference when compared with the urea group (P>0. 05). Conclusion:The extract of Ginkgo Biloba has a certain effect on the back chapped skin in SD rats, which may contribute to the external preparations of Ginkgo Biloba extract.
4.Teaching Management with Credit System in Medical Colleges and Universities
Ge CHEN ; Zhifen LI ; Haiying SHAO
Chinese Journal of Medical Education Research 2003;0(02):-
With the development of higher medical education,the traditional academic year system can no longer meet the development of the times.This paper analyzes the advantages of the Credit System and the difficulties met by Medical Colleges and Universities that apply Credit System Management Mode,and make some proposals to the measures of the credit system management.
5.Congenital chloride diarrhea in a case.
Shao-Jie YUE ; Fei-Ge TANG ; Xia WANG ; Yujia YANG
Chinese Journal of Pediatrics 2005;43(1):71-72
Chlorides
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metabolism
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Diarrhea
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congenital
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Humans
6.Endoscopic CO2 laser surgery with postcricoid advancement flap for glottic stenosis.
Xiao-Mei SU ; Ping-Jiang GE ; Shao-Hua CHEN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2011;46(1):69-70
Adult
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Female
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Glottis
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Humans
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Laryngeal Mucosa
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transplantation
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Laryngoscopy
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Laryngostenosis
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surgery
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Lasers, Gas
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Male
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Middle Aged
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Reconstructive Surgical Procedures
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methods
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Young Adult
8.Overview on the New Dosage Forms of Sirolimus
Mingjie PAN ; Rong DU ; Ying CHEN ; Ge SHAO
China Pharmacist 2016;19(6):1183-1186
In the middle of 1990 s, it was discovered that sirolimus had inhibitory effect on the proliferation of T lymphocytes , therefore, it is used for the treatment of anti-tumor cells.At present, the dosage forms of sirolimus are capsules , oral liquid and tab-lets.However, because of good fat-soluble and poor water-soluble property, its bioavailability is low with many side reactions .In order to provide reference for the further studies of sirolimus , the literatures in recent years were searched in Pubmed , Springer, CJFD,VIP Chinese periodical database and Wanfang data , and the research progress in the new dosage forms of sirolimus was summarized .
9.Discussion on cultivation of general practitioner at primary levels based on the theory of post competency
Zhujian SHAO ; Chunming LI ; Xiaolong QIN ; Zhenglong GE ; Qi CHEN
Chinese Journal of Medical Education Research 2016;15(3):226-229
The cultivation of general practitioner is a key to improve China's community-based medical and health care system.Zunyi Medical University,in the process of cultivating tuition-free students with the mode of serving and staying in the countryside after their graduation,always upholds the principles of being oriented by post competence,being led by medical humanities,optimizing featured courses about general practitioner,carrying out students-centered teaching methodology,focusing on training students' autonomous learning ability,and fully building the practical teaching system,so as to strive to cultivate high-quality talents in the field of general medicine to make them better serve the rural community-based medical institutions.
10.A correlation between osteophytes in knee-joint and lower limb alignment in adult patients with Kaschin-Beck disease
Guohua CHEN ; Yongjian LIAO ; Pengfei GE ; Jianyun SHAO
Chinese Journal of Endemiology 2015;34(10):739-741
Objective To evaluate the correlation between osteophytes size and lower limb alignment in the knees of patients with Kaschin-Beck disease (KBD).Methods A total of 300 clinically diagnosed patients with KBD were X-rayed on knee-joints which ranged from the distal half of femur to proximal half of tibia.Meanwhile some related parameters in the X film with the anteroposterior position (including osteophytes length,femorotibial angle,femorotibial joint space ratio of inner side to outer side) were measured by DICOM 2.0,a software of medical graphic measuring,then followed by calculating the osteophyte spur index.The association between femorotibial angle,femorotibial joint space ratio and osteophytes spur index was evaluated by Pearson correlation test.Results The average of femorotibial angle of all the tested knee-joints was (165.97 ± 4.02)°,which positively correlated with both the osteophyte spur index of the medial femoral condyle [(6.54 ± 3.12)%,correlation coefficient (r) =0.524,P<0.01] and the osteophyte spur index of medial tibil plateau [(7.14 ± 3.40)%,r =0.578,P <0.01].The femorotibial joint space ratio was 0.61 ± 0.13,which positively correlated with both the osteophyte spur index of medial femoral condyle (r =0.531,P <0.01) and that of the medial tibil plateau (r =0.563,P <0.01).Conclusions The results of this study indicate that there is a positive correlation between lower limb alignment and osteophyte size of both the medial femoral condyle and the medial tibial plateau.This finding may be evidenced by the fact that the changes of lower limb biomechanics may contribute to formation and development of osteophytes in the kneejoint.