Objective To observe the effect of marin algae polysaccharide derivant on the osteoporotic model of the rats. Methods Forty six-month-old female Wistar rats were selected to duplicate the osteoporotic model induced by retinoic acid and performed with marine algae polysaccharide derivant. The rats were randomly divided into two groups: experiment group treated with 10 mg marine algae polysaccharide derivant10mg per day by oral,control group treated with 10 mg glucose per day by oral.The effect of marine algae poly saccharide derivant on osteoporosis were evaluated by morphometry. Results After intake of polysaccharide,the mean trabecular plate density (Tb.N),the mean trabecular plate thickness(Tb.Th),and the percent trabecular area (Tb.Ar%) of t he experiment group, which were treated by polysaccharide were significantly increased more than that of the control group model(P<0.01). Conclusion These results indicate that polysaccharide can increase bone mass and has a therapeutic and preventional effect on the osteoporotic model of the rats induced by retinoic acid.

Objective To establish a method for determination of trace lead in drinking water by intermittent flow HG-AFS was developed. Methods The conditions for K3Fe(CN)6-KBH4-HCl system of generation of PbH4 were optimized and interference of coexisting ions were tested. Results The fluorescence signal of blank solution was decreased evidently by treating K3Fe(CN)6 with activated carbon and the detection limit was effectively improved. The interference of coexisting ions could be suppressed in some degrees in the presence of K3[Fe(CN)6]. Under the optimized conditions, the detection limit of this method was 0.10 ?g/L and RSD(n=6) was in the rang 0.4%-3.8%. The recovery rate was 95.0%-112.0%. Conclusion This method was accurate, simple, sensitive and suitable for determination of trace lead in drinking water.

The same paraffin-embedded specimen of skin biopsy from each of 29 patients with cutaneous malignant lymphomas (CML) and 7 patients with cutaneous lymphoid infiltrates (CLI) was estimated simultaneously and retrospectively by karyomorphometry and proliferating rate assay. The results showed that the differences in nuclear volumes (NVs), count of argyrophilic nucleolar organizer regions(AgNORs) and proliferating cell nuclear antigen (PCNA-PC10) indices (PC10Is) between CML and CLI were statistically very significant. The NVs and PC10Is were correlated to the death rate of patients. NVs were also correlated to AgNORs.

Objective:To translate the English version of Edmonton Frail Scale into Chinese version and to test its reliability and validity.Methods:The translation and cross-cultural adaptation of the English version of Edmonton Frail Scale were generated by Beaton's translation and back-translation method. From April to August of 2019, at the department of geratology of the First affiliated Hospital of China Medical University in Shenyang, a sample of 303 hospitalized elderly adults who met the inclusion and exclusion criteria were selected via convenience sampling method to test its psychometric properties.Results:The scale level content validity index and item level content validity index of the Chinese version of the Edmonton Frail Scale were both 1.0. The criterion validity between the Chinese version of Edmonton Frail Scale and the Tilburg Frailty Indicator was 0.723 ( P<0.01), confirmed by Pearson correlation coefficients. Using the Tilburg Frailty Indicator as an external criterion, the Edmonton Frail Scale showed satisfactory diagnostic accuracy for frailty (area under the curve=0.924). The optimal cut-point for frailty was 6 (sensitivity: 77.6%, specificity: 94.7%). More frail individuals were recognized by the Chinese version of Edmonton Frail Scale among older and female participants than their counterparts( P<0.05). The Cronbach'α of the Chinese version of Edmonton Frail Scale was 0.599, the test-retest reliability within a 7-day interval of the scale was 0.822. Conclusions:The Chinese version of Edmonton Frail Scale presents good validity and reliability and can apply to Chinese hospitalized elderly people.

Objective: To explore a scientific, complicated sampling method and corresponding formulas for sensitive question survey. Methods: Cochran W. G.'s classic sampling theories, two randomized response technique(RRT) models, total probability formulas, and properties of mean and variance were used in this study. Results: Formulas for the estimation of the population proportion and its variance for dichotomous sensitive questions on Greenberg model in cluster sampling and stratified cluster sampling were deduced. Formulas for the estimation of the population mean and its variance for quantitative sensitive question on the additive constant model in cluster sampling and stratified cluster sampling were deduced. Our survey methods and formulas were successfully applied in surveying the pre-marriage sex and cheating in examination in Soochow University. Conclusion: Our survey methods and formulas on two RRT models for sensitive question survey in cluster sampling and stratified cluster sampling have high reliablility.

The peak of the brain edema after intracerebral hemorrhage usually begins after 3 to 4 days.A large quantity of hemoglobin releases and degrades during this process,and its degradation products iron ion,bilirubin and carbon monoxide,etc play important roles in the process of cerebral edema.They all involve in the formation Of delayed brain edema after intracerebral hemorrhage.The research of hemoglobin and its degradation products is expected to find out a new way for the treatment of intracerebral hemorrhage.

Objective To study the correlativity of laser-induced fluorescence (LIF) spectrum on the same specimen of colorectal carcinoma both in vivo and in vitro and to obtain the best diagnostic parameter in detecting colorectal carcinoma. Methods LIF spectra were analyzed from 10 patients with colorectal carcinoma during colonoscopy, and then analyzed again after resection. The cancer foci and normal mucosa more than 5 cm apart from the foci were excited by a nitrogen laser (337 nm)using a quartz optical fiber in gentle contact with the area of interest, and then the fluorescence emission was collected by the same quartz optical fiber. The LIF spectrum was analyzed using an optical multichannel analyzer (OMAⅢ). Results (1)The LIF spectrum from cancer tissues was same as that of normal tissues, both showed the main and the second peak respectively located at about 460 nm and 390 nm in vivo and in vitro. The main peak intensities in vivo from both cancer and normal tissue were significantly lower than these in vitro. The main peak intensities in vivo and in vitro from cancer tissue were decreased about 1 time to those from normal tissue.(2) The integrated spectral intensity in vivo and in vitro of cancer tissue from each patient was significantly lower than that from the corresponding normal tissue respectively, P

OBJECTIVE:To establish HPLC method for the simultaneous determination of calcium lactate and calcium gluconate in compound calcium gluconate oral solution.METHODS:The test was performed on C 18 column with column tem?perature at25℃;the mobile phase was composed of0.025mol/L NaH 2 PO 4 (pH=2.50)with a flow rate of1.0ml/min and sam ple size of20?l;the detection wavelength was210nm.RESULTS:The calibration curves were linear over the range of0.719～46.040mmol/L(r=0.9999)for lactic acid and0.315～20.160mmol/L(r=0.9999)for gluconic acid.The average recovery rates of which were99.0%(RSD=1.65%)and100.5%(RSD=1.36%),respectively.CONCLUSION:This method is fast,simple,accurate,and it can be employed directly without isolation to determine the content of calcium lactate and calcium glu?conate simultaneously and to control the quality of compound calcium gluconate oral solution as well.

Studies were carried out on the incidence of SCE and chromosomal aberrations in primary cultured cells of mouse embryo exposed to ~(60)Co-? rays and/or treated with chemical carcinogen——benzopyrene.Result shows in the cultured cells exposed to ~(60)Co-? rays at 50 rads, as compared wtih controls there was no statistically significant change in the frequencies of the appearance of both SCE and chromosomal aberrations, In the cells, however, exposed at 300 rads, the frequencies of SCE and chromosomal aberrations were about 1.3 and 24 times higher than that in the unirradiated controls respectively. In the cultured cells treated with 0.05?g of benzopyrene per millilitre of culture medium, the frequency of SCE were increased by about 1.8 times of that of the control group, while the change of chromosomal aberrations had no statistical significance.From the above results, it was suggested that SCE was proved to be a sensitive indicator for evaluating the effects of chemical carcinogens. There is a remarkable difference existing between the frequencies of SCE and chromosomal aberrations induced by ionizing radiation and chemical carcinogens. The difference seems to have a certain relation to their differences in carcinogenic mechanism and effects on carcinogenesis.It was noted that there are some advantages to utilize primary cultured cells of mouse embryo as target cells.

0.999). The characteristic masses and detection limits achieved were as follows,as for As,21 pg and 0.13 ?g/L,as for Se,32 pg and 0.33 ?g/L. The recoveries were 96.0%-103.6% for As,98.3%-104.7% for Se and the RSDs were 0.6%-1.7% for As,0.6%-2.8% for Se. Conclusion This method was accurate, simple and sensitive,it is applicable to the determination of trace As and Se in water.