Objective To observe the effect of marin algae polysaccharide derivant on the osteoporotic model of the rats. Methods Forty six-month-old female Wistar rats were selected to duplicate the osteoporotic model induced by retinoic acid and performed with marine algae polysaccharide derivant. The rats were randomly divided into two groups: experiment group treated with 10 mg marine algae polysaccharide derivant10mg per day by oral,control group treated with 10 mg glucose per day by oral.The effect of marine algae poly saccharide derivant on osteoporosis were evaluated by morphometry. Results After intake of polysaccharide,the mean trabecular plate density (Tb.N),the mean trabecular plate thickness(Tb.Th),and the percent trabecular area (Tb.Ar%) of t he experiment group, which were treated by polysaccharide were significantly increased more than that of the control group model(P<0.01). Conclusion These results indicate that polysaccharide can increase bone mass and has a therapeutic and preventional effect on the osteoporotic model of the rats induced by retinoic acid.

The same paraffin-embedded specimen of skin biopsy from each of 29 patients with cutaneous malignant lymphomas (CML) and 7 patients with cutaneous lymphoid infiltrates (CLI) was estimated simultaneously and retrospectively by karyomorphometry and proliferating rate assay. The results showed that the differences in nuclear volumes (NVs), count of argyrophilic nucleolar organizer regions(AgNORs) and proliferating cell nuclear antigen (PCNA-PC10) indices (PC10Is) between CML and CLI were statistically very significant. The NVs and PC10Is were correlated to the death rate of patients. NVs were also correlated to AgNORs.

Objective To establish a method for determination of trace lead in drinking water by intermittent flow HG-AFS was developed. Methods The conditions for K3Fe(CN)6-KBH4-HCl system of generation of PbH4 were optimized and interference of coexisting ions were tested. Results The fluorescence signal of blank solution was decreased evidently by treating K3Fe(CN)6 with activated carbon and the detection limit was effectively improved. The interference of coexisting ions could be suppressed in some degrees in the presence of K3[Fe(CN)6]. Under the optimized conditions, the detection limit of this method was 0.10 ?g/L and RSD(n=6) was in the rang 0.4%-3.8%. The recovery rate was 95.0%-112.0%. Conclusion This method was accurate, simple, sensitive and suitable for determination of trace lead in drinking water.

Objective:To translate the English version of Edmonton Frail Scale into Chinese version and to test its reliability and validity.Methods:The translation and cross-cultural adaptation of the English version of Edmonton Frail Scale were generated by Beaton's translation and back-translation method. From April to August of 2019, at the department of geratology of the First affiliated Hospital of China Medical University in Shenyang, a sample of 303 hospitalized elderly adults who met the inclusion and exclusion criteria were selected via convenience sampling method to test its psychometric properties.Results:The scale level content validity index and item level content validity index of the Chinese version of the Edmonton Frail Scale were both 1.0. The criterion validity between the Chinese version of Edmonton Frail Scale and the Tilburg Frailty Indicator was 0.723 ( P<0.01), confirmed by Pearson correlation coefficients. Using the Tilburg Frailty Indicator as an external criterion, the Edmonton Frail Scale showed satisfactory diagnostic accuracy for frailty (area under the curve=0.924). The optimal cut-point for frailty was 6 (sensitivity: 77.6%, specificity: 94.7%). More frail individuals were recognized by the Chinese version of Edmonton Frail Scale among older and female participants than their counterparts( P<0.05). The Cronbach'α of the Chinese version of Edmonton Frail Scale was 0.599, the test-retest reliability within a 7-day interval of the scale was 0.822. Conclusions:The Chinese version of Edmonton Frail Scale presents good validity and reliability and can apply to Chinese hospitalized elderly people.

Objective: To explore a scientific, complicated sampling method and corresponding formulas for sensitive question survey. Methods: Cochran W. G.'s classic sampling theories, two randomized response technique(RRT) models, total probability formulas, and properties of mean and variance were used in this study. Results: Formulas for the estimation of the population proportion and its variance for dichotomous sensitive questions on Greenberg model in cluster sampling and stratified cluster sampling were deduced. Formulas for the estimation of the population mean and its variance for quantitative sensitive question on the additive constant model in cluster sampling and stratified cluster sampling were deduced. Our survey methods and formulas were successfully applied in surveying the pre-marriage sex and cheating in examination in Soochow University. Conclusion: Our survey methods and formulas on two RRT models for sensitive question survey in cluster sampling and stratified cluster sampling have high reliablility.

Objective To explore the classical clinical features of mitochondrial encephalomyopathy with lactic acidosis and stroke like episodes (MELAS) syndrome.Methods The clinical data of 1 MELAS syndrome patient was analyzed retrospectively.Results This patient suffered from the symptoms of stroke such as speech disorder and abnormal behavior at the beginning.She had a medical history of maternal family.There were headache and seizure in the process of the disease.The result after a physical examination showed that the patient had hearing loss, visual field defect and decrease of muscle strength.The CT and MRI scan of head showed that the patient had a stroke like lesion which did not follow the distribution of blood vessels.The blood and cerebrospinal fluid examination has ruled out viral and autoimmune encephalitis.The final diagnosis of MELAS syndrome was confirmed by gene analysis.The gene mutation was the m.3243A>G mutation.The clinical symptoms of the patient were comprehensive and the imaging findings were typical.Conclusions Main clinical manifestations of MELAS syndrome are stroke like seizures, epilepsy, headache, dementia, hearing impairment, peripheral neuropathy, myopathy, lactic acidosis, diabetes and so on.Main imaging features of the disease are stroke like lesions, basal ganglia calcification and brain atrophy.

Objective To investigate the clinical application of serum retinal binding protein and cystain C determination in hem-orrhagic fever with renal syndrome(HFRS).Methods The serum concentrations of RBP,CysC,Urea and Cr were detected for 124 patients with HFRS(patients group)and 100 healthy people who underwent physical examination during the same period(control group),the date were analysed by SPSS19.0 software.Results The concentrations of RBP,CysC,Urea and Cr increased signifi-cantly in febrile stage of HFRS,arrived at peak in oliguria stage,and then declined gradually,there were statistically significant differences between each stage and negative control stage(P <0.05).RBP and CysC had a good positive correlation with Urea and Cr,the correlation coefficient between RBP and Urea or Cr were 0.826 and 0.892,respectively(P <0.05)while with CysC were 0. 841 and 0.924,respectively(P <0.05).The positive rates of RBP,CysC,Urea,Cr in febrile stage were 85.48%,95.16%,69.35%, 83.06% respectively,while in convalescent stage were 67.74%,74.19%,46.77% and 58.06% respectively.Conclusion RBP and CysC are good indicators for diagnosing HFRS,which also have good correlation with Urea and Cr,which are recommended in clini-cal application.

AIM: Eye surface burn is easy to cause blindness. Amniotic membrane transplantation has become a hot spot of research for its special effects in recent years. This study observed the therapeutic outcome of fresh amniotic membrane transplantation for acute chemical or thermal burns at eye surface. METHODS: ①Eight eyes with acid burn in eight cases, ten eyes with alkali burn in ten cases, and seven eyes with thermal burn in six cases were selected from Yan'an University Affiliated Hospital between January 2002 and June 2007 including 23 males and 1 female aged 16-50 years. There were 18 eyes with preoperative vision

BACKGROUND:Heterogeneous deproteinized bone because of wide source and its special biological characteristics maybe a good bone tissue engineering scaffold.Its immunogenicity and mechanical properties are different by different interventions.OBJECTIVE:To proof the preparation technology,immunological and mechanical properties of heterogeneous deproteinized bone.DESIGN,TIME AND SETTING:A control observational experiment was performed in the Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy from February to October 2008.MATERIALS:Fresh femoral cancellous bones extracted from adult pigs,which were not including cartilages and cortical bones,were prepared into size of 3 cm?0.5 cm?0.5 cm.Their long axis direction was the same with orientation of trabeculae.The apparent pore densities of the used bones were almost same.METHODS:The bones were soaked in chloroform-carbinol(1:1) 24 hours for degrease.They were shaken and washed by distilled water at 50 ℃,and then put into 20% H2O2 for 24 hours.The procedure repeated three times.The bones were soaked in distilled water at room temperature for 24 hours,and then dryed.MAIN OUTCOME MEASURES:The gross morphological and histological features of the deproteinized cancellous bones were observed.The contents of amino acid and their biomechanical properties were measured.RESULTS:The three-dimensional space structures of the deproteinized cancellous bones were not damage greatly,and they had a natural pore network system which composited of hydroxyapatite and collagen.The contents of collagen amino acids in the deproteinized cancellous bones had no obvious difference from fresh cancellous bones,but the wave crests of aromatic amino acids such as tyrosine and methionine were disappear.The elastic moduli of deproteinized cancellous bones were significantly higher than those of fresh cancellous bones(P

BACKGROUND:Deproteinized cancellous bone can be used as a new kind of bone graft material due to its wide resources andbiological characteristics if the problem of immunogen can be solved. OBJECTIVE:To study the properties of deproteinized cancellous bone following preparation with physico-chemical methods,in addition,to explore the feasibility of xenogeneic deproteinized cancellous bone used as scaffolds in bone tissue engineering. DESIGN,TIME AND SETTING:A comparison observation was performed at the Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy between February 2008 and January 2009. MATERIALS:Deproteinized bone was derived from pigs and goals femoral cancellous bone followed by treating with 1:1 methanol extract and 30% H2O2. METHODS:The mechanical properties of vector were examined by scanning electron-microscopy,X-rays diffraction analysis,differential scanning calorimetry,and mechanical experimental instrument. The density of third passage of human bone mesenchymal stem cells (MSCs) of iliac bone was regulated to 2?108/L,vaccinated to vector,and the cell adhesion and proliferation was observed by inverted microscope and scanning electron microscope. Forty BALB/C mice were randomly dividedinto the experimental and control groups,with 20 animals in each group. Muscle behind femur was made muscle pouch,which was implanted pig deproteinized bone in the experimental group,and no other treatment in the control group. The level of serumcarnine,glutamic-oxal(o)acetic transaminase (GOT),as well as glutamic-pyruvic transaminase (GPT) were measured at weeks 12,4,8 after operation. Meanwhile,the immunogenicity of pig deproteinized bone and specimen of deproteinized bone in mice were detected at the 4 weeks after implantation. MAIN OUTCOME MEASURES:Physicochemical properties and immunogenicity of deproteinized bone were analyzed by in vitroexperiment. And the bio-safety was evaluated by animal experiment. RESULTS:The inorganic composition of deproteinized cancellous bone was hydroxyapatite ceramic,which is identical with nature composition of human bone. It showed that deproteinized cancellous bone maintained natural network pore system and kept good mechanical property. The MSCs grew well on deproteinized cancellous bone due to its low immunogenicity and toxicity. CONCLUSION:The xenogeneic deproteinized cancellous bone is an excellent vector in bone tissue engineering.