Transgender people face many psychological problems due to not fitting into the binary categories (men and women) in Pakistani society. The current study aimed to explore the role of ostracism in subjective well-being (like life satisfaction, positive and negative affect) among transgender. The correlational research design was used in the present study. Through purposive and snowball sampling techniques, the 109 transgender sample was selected from Faisalabad and Lahore city. Urdu versions of the General Ostracism Scale, negative and positive affect scale and satisfaction with life scale were used as study measures. Data were analyzed through pearson product moment correlation, linear regression analysis and Multivariate Analyses of Variance (MANOVA). The results on correlation analysis show a significant positive relationship between ostracism and the subjective well-being component of negative affect. The findings on linear regression analysis revealed that ostracism was a significant positive predictor of negative affect in the transgender sample accounting for 11% of the variance in the model. The results on MANOVA showed substantial differences in life satisfaction and positive affect in terms of education levels. The current study findings can be utilized in various NGOs and mental health settings. They can teach the transgender community different coping strategies to deal with ostracism that will improve the levels of transgender's overall subjective well-being.