ObjectiveTo investigate whether single nucleotide polymorphism (SNP) of macrophage migration inhibitory factor (MIF) gene - 173G/C is associated with severe preeclampsia.Methods Totally 124 severe preeclampsia patients and 160 healthy pregnant women (control group) were included in our study who were recruited consecutively from Affiliated Hospital of Qiugdao University Medical College between March 2010 and March 2011.The SNP was detected through SYBR Green PCR.The levels of fasting blood glucose ( FBG),fasting insulin ( FIN),and serum total cholesterol (TC),triglyceride ( TG),high density lipoprotein (HDL) and light density lipoprotein (LDL) were determined in every participants.The homeostasis model assessment-insulin resistance (HOMA-IR) was calculated.The allele and genotype frequencies between severe preeclampsia patients and control group were compared.The FBG,FIN,body mass index (BMI),HOMA-IR,TC,TG,HDL and LDL in different genotype were compared.Results ( 1 ) The MIF - 173G/C SNP genotype frequencies of GG,CG,and CC were 62.1％ (77/124),30.6％ (38/124),7.3％ (9/124),the allelic frequencies of G and C were 77.4％ ( 192/248 ) and 22.6％ (56/248),respectively,in severe preeclampsia patients; the MIF - 173G/C SNP genotype frequencies of GG,CG,and CC were 64.4％ ( 103/160 ),30.6％ (49/160),5.0％ ( 8/160),the allelic frequencies of G and C were 79.7％ (255/320) and 20.3％ (65/320),respectively,in the control group.No significant differences were observed in the genotypes and allele distributions of MIF - 173G/C SNP between the severe preeclampsia patients and control group (all P ＞ 0.05 ).(2) The severe preeclampsia patients with CG and CC genotypes had higher BMI compared with the GG genotype [ (25 ±4) versus (22 ±4) kg/m2 ; t =3.96,P ＜ 0.05 ].( 3 ) The severe preeclampsia patients with CG and CC genotypes had higher FIN level and higher HOMA-IR compared with the GG genotype [ ( 15.7 ±2.9) versus ( 13.6 ±4.0) mmoL/L,3.3 ±0.5 versus 2.7 ± 0.6 ; t =3.17,t =5.58,all P ＜ 0.05 ].(4) There was no significant difference in FBG,TC,TG,HDL and LDL levels in severe preeclampsia patients with different genotypes (all P ＞0.05 ).Conclusions The present study suggests that the MIF - 173G/C SNP is associated with insulin resistance in severe preeclampsia patients.The CG and CC genotypes increase the degree of insulin resistance,but it is may not associate with susceptibility among severe preeclampsia patients of Han Chinese women.

【Objective】 To explore the risk factors that affect the outcome of testicular torsion in patients with testicular resection, to provide ideas for clinical management. 【Methods】 The clinical data of 117 patients with testicular torsion treated in our hospital during Jan.1, 2012 and Mar.1, 2023 were retrospectively analyzed, 43 of whom underwent testicular detorsion and orchidopexy, and 74 underwent orchiectomy.The correlation between preoperative inflammatory and coagulation-related indicators, testicular torsion angle, TWIST score and orchiectomy was analyzed with univariate analysis.The independent risk factors for testicular torsion patients with testicular resection as the outcome were analyzed with binary logistic regression.The predictive value of each factor for orchiectomy was assessed with receiver operating characteristic (ROC) curve. 【Results】 Binary logistic regression analysis indicated that long duration of onset (OR=1.841, P=0.036), large torsion angle (OR=1.005, P=0.016), high TWIST score (OR=2.225, P=0.003) and elevated FIB level (OR=2.489, P=0.049) were independent risk factors for testicular torsion patients with testicular resection as the outcome.ROC curve analysis showed that the best cut-off value of the above 4 factors to predict orchiectomy were 1.75 days, 225°, 3.5 and 3.155 g/L; the area under the ROC curve (AUC) were 0.893, 0.718, 0.812 and 0.770, respectively. 【Conclusion】 The duration of onset, torsion angle, TWIST score, and FIB level are independent risk factors for testicular torsion patients with testicular resection as the outcome, and these indicators have certain predictive value.

【Objective】 To investigate the clinicopathological features and key points of diagnosis and treatment of malignant perivascular epithelioid cell tumor (PEComa) to increase awareness of the disease. 【Methods】 The clinicopathological data of 2 patients with malignant PEComa treated in our hospital were retrospectively analyzed, and relevant literatures were reviewed. 【Results】 Both patients were male, aged 53 and 16 years, respectively.The sites of occurrence were in the retroperitoneum and pelvis, respectively.Both tumors were resected surgically, and the diagnosis was confirmed with postoperative pathology.Under the microscope, the tumor tissue of one patient was mainly composed of smooth muscle-like cells, and that of the other patient was composed of epithelioid cells, both showing pathological mitotic images and expressing HMB45, Melan-A, SMA and CD34, no tumor recurrence or metastasis was observed during the follow-up.The literatures collected involved 15 patients with retroperitoneal or pelvic PEComa, including 3 males and 12 females, of which 9 were malignant.The clinical manifestations were abdominal pain, bloating, or lower back pain.Some cases were detected during physical examinations. 【Conclusion】 Malignant PEComa is difficult to be diagnosed before surgery and easy to be misdiagnosed.The confirmed diagnosis depends on the postoperative pathological results.The preferred treatment is complete resection of tumor.Long-term follow-up is needed.