AIM: To explore the kinetics and thermodynamics parameters in the extraction of flavonoids from Fagopyrum tartarjcum . METHODS: Rutin was adopted as marker substance to create a regression equation between Rutin concentration and absorbance by meaus of UV. RESULTS: According to Fick diffusion principle Ⅱ, extraction process was analysed to gain parameters, such as K, Ea, Y, t 1/2 , K ?. CONCLUSION: The method would be useful for the process design and the choice of operation condition to extract flavonoids from Fagopyrum tartarjcum.

AIM: To use secondary regression coherent design to explore the extraction parameters of fruits of Cnidiunm by supercritical CO_2 extraction. METHODS: Extraction pressure,extraction temperature,isolation pressure,isolation temperature Ⅰ and Ⅱ were viewed as factors. RESULTS: The best technological conditions were: extractive kettle pressure was 20 MPa,extractive kettle temperature was at 40(?C);separate kettle Ⅰ pressure was 15 MPa,separate kettle Ⅰ temperature was at 65(?C);separate kettle Ⅱ pressure was 5 MPa,separate ketle Ⅱ temperature was at 38(?C);CO_2 flow rate was 18 L/H,extractive time was 80 min. CONCLUSION: Under these conditions,the separate rate of the extractives from fruits of Cnidium Monnieri(L.) Cuss.could be(24.03%).Using secondary regression coherent design could naturally react the influence of the rule on the every factor to the goal function.

Polylactide is a biodegradable and biocompatible biomaterial. Based on the PLA (Polylactide) membrane, we have produced a new PLA/PTMC (Polytrimethylene carbonate) blends membrane. The biological properties of this membrane were studied by cell toxicity experiment, acute toxicity experiment, skin irritant experiment, sensitization test, hemolytic test, micronucleus test and subcutaneous implantation test. The results demonstrated that the blends membrane has no toxicity and it does not cause skin irritation, hypersensitive reaction and hemolysis. The micronucleus ratio of the membrane is 1.3% +/- 1.0%, being less than 3%. The result of medullary micronucleus test was reported negative. The wounds were free from suppuration and necrosis after subcutaneous implantation in all periods. In the experimental application of this member to preventing adhesion after rabbit intestine operation, the membrane demonstrated good effect. In conclusion, PLA/PTMC blends membrane is a material with good biocompatibility.
Animals ; Biocompatible Materials ; chemistry ; Dioxanes ; chemistry ; Female ; Intestinal Diseases ; prevention & control ; surgery ; Male ; Materials Testing ; Membranes, Artificial ; Mice ; Polyesters ; chemistry ; Polymers ; chemistry ; Rabbits ; Random Allocation ; Tissue Adhesions ; prevention & control

Chronic myeloid leukemia (CML) is a slowly progressing hematopoietic cell disorder. Sphingosine kinase 1 (SPHK1) plays established roles in tumor initiation, progression, and chemotherapy resistance in a wide range of cancers, including leukemia.However, small-molecule inhibitors targeting SPHK1 in CML still need to be developed. This study revealed the role of SPHK1 in CML and investigated the potential anti-leukemic activity of hirsuteine (HST), an indole alkaloid obtained from the oriental plant Uncaria rhynchophylla, in CML cells. These results suggest that SPHK1 is highly expressed in CML cells and that overexpression of SPHK1 represents poor clinical outcomes in CML patients. HST exposure led to G2/M phase arrest, cellular apoptosis, and downregulation of Cyclin B1 and CDC2 and cleavage of Caspase 3 and PARP in CML cells. HST shifted sphingolipid rheostat from sphingosine 1-phosphate (S1P) towards the ceramide coupled with a marked inhibition of SPHK1. Mechanistically, HST significantly blocked SPHK1/S1P/S1PR1 and BCR-ABL/PI3K/Akt pathways. In addition, HST can be docked with residues of SPHK1 and shifts the SPHK1 melting curve, indicating the potential protein-ligand interactions between SPHK1 and HST in both CML cells. SPHK1 overexpression impaired apoptosis and proliferation of CML cells induced by HST alone. These results suggest that HST, which may serve as a novel and specific SPHK1 inhibitor, exerts anti-leukemic activity by inhibiting the SPHK1/S1P/ S1PR1 and BCR-ABL/PI3K/Akt pathways in CML cells, thus conferring HST as a promising anti-leukemic drug for CML therapy in the future.