Objective: To establish the quality standard for Ruhexiao Capsules(Spica Prunellae,Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Angelicae sinensis,Bombyx Batryticatus, Radix Bupleuri, Rhizoma Corydalis, Carapax Trionycis, etc.) Methods: The Spica Prunellae, Radix Angelicae sinensis, Rhizoma chuanxiong, Radix Bupleuri and Rhizoma Corydails of Ruhexiao Capsules were identified by TLC. Paeoniflorin of the capsules was determined by HPLC. The mobile phase was methanol water(17∶38), flow rate 0.8mL?min -1 . The detection wavelength was 230nm. Results: The linear range of paeoniflorin was 0.532?g～2.66?g, the average recovery rate was 98.29% and RSD was 0.97%, respectively. Conclusion: This method was simple. The results were reliable and accurate. The method can be used for quality control of Ruhexiao Capsules.