Atherectomy, Coronary ; methods ; Drug-Eluting Stents ; Humans

Objective:To observe the inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG2 and investi-gate its possible mechanisms. Methods:HepG2 cells were treated with different concentration of disodium cantharidinate,The in- hibiton of cell proliferation,cell cycle distribution,expression of Survivin and activity of Caspase-3 were respectively evaluated by MTT assay,flow cytometry,immunocytochemistry,and chromatometry. Results:Disodium cantharidinate could inhibit the proliferation of HepG2 cells. The IC50 at 24,48,72 h after intervention were 2.41?0.48,0.72?0.08,and 0.39?0.04 ug/ml,The cells of G2/M phase and activity of Caspase-3 were increased.the expression respectively of Survivin were inhibited after the intervention of disodium cantharidinate. Conclusion:The inhibitory effect of disodium cantharidinate on HepG2 cells may be mediated by the block of cellcycle,enhancement of Caspase-3,and inhibition of Survivin.

Objective To observe the clinical efficacy of Yang Xin Huo Xue(nourishing the heart and activating blood flow) needling method plus Western medication in treating chronic heart failure due to coronary heart disease (CHD).Method Eighty patients with CHD-induced chronic heart failure were randomized into a treatment group and a control group, to respectively receiveYang Xin Huo Xueneedling method plus Western medicationor Western medication alone for intervention. The heart function was estimated and brain natriuretic peptide (BNP) was detected before and after the intervention in both groups, and the change of heart function was observed.Result Yang Xin Huo Xueneedling method plus Western medication achieved a satisfactory therapeutic efficacy in treating chronic heart failure, and its total effective rate was significantly different from the rate in the control group (P<0.05); compared with the control group, the BNP value declined more significantly in the treatment group, and the difference was statistically significant (P<0.05).Conclusion Yang Xin Huo Xueneedling method plus Western medication can produce a satisfactory efficacy in treating CHD-induced chronicheart failure.

A L-serine producing facuhative methylotroph mutant (Mth~H、Gly~R)was derived from Pseudomonas flava A3 by combination treatment with ultraviolet(UV)and diethylsulfate(DES).It could accumulate 6.2g/L L-serine in the medium containing 30g/L glycine and 1% methanol as carbon source when it was cultured for 3 days,and it has an improvement about 67.6% compared with the origin.The mutant also has a good stability of descendiblity of L-serine producing.

Fracture Fixation ; instrumentation ; methods ; Fractures, Bone ; surgery ; Humans ; Scaphoid Bone ; injuries ; surgery ; Wrist Injuries ; surgery ; Wrist Joint ; surgery

Primary CNS lymphoma (PCNSL) is a rare malignancy with peculiar clinical and biologic features,aggressive course,and unsatisfactory outcome.the particular microenvironment of this malignancy,and sanctuary sites where tumor cells grow undisturbed, strongly affects treatment efficacy. The execution of prospective trials is also difficult because of the rarity of the tumor and the impaired general condition and poor performance status of patients. Chemotherapy is indispensable for the treatment of PCNSL. High-dose methotrexate is the most effctive drug.PCNSL is sensitive to irradiation,but responses to radiotherapy alone are short-lived. MTX-based chemotherapy followed by whole-brain radiotherapy prolonged survival but is associated with delayed neurotoxicity especially in patients older than 60 years. A new approach is attempted to treat PCNSL with chemotherapy alone with defered radiotherapy in old patients with similar survival rencenfly. High-dose chemotherapy with autologous stem cell transplantation HDC/ASCT yielded promising results for recurent PCNSL.Clinical investigation with Temozolomide or rituximab have been reported.Further studies with these new drugs are needed.

SUMMARY This case report concerns a 23-year-old woman with chronic periodontitis who had been pre-viously diagnosed with Takayasu arteritis ( TA ) .Her erythrocyte sedimentation rate ( ESR ) and C-reactive protein ( CRP) level were decreased before and 3 months after non-surgical periodontal therapy with no change in her prescribed medications (ESR from 31.00 mm/h to 23.0 mm/h, CRP from 21.40 mg/L to 18.46 mm/h).Through the occasional findings , we raised a hypothetical analysis about the po-tential relationship between periodontitis and TA with respect to inflammatory factors , bacteria and medi-cation.Further studies of large samples are needed to verify the findings .

Objective　To study the factors effecting the formation of the ventricular aneurysm post myocardial infarction (MI) and evaluate the diagnostic methods.Methods　23 MI patients with ventricular aneurysm confirmed by left ventricular angiography were observed for electrocardiogram (ECG), echocardiogram (ECHO), coronary disease and collateral vessels formation. Results　All patients had LAD disease, 65.2% patients had three coronary disease, and those with class Ⅲ collateral vessels circulation only 17.4%. Compared to ECHO by which only 4 patients were found aneurysm, the positive rate by ECG was 82.6%. Conclusion　Patients with multiple coronary disease and without enough collateral vessels are expected to form ventricular aneurysm. ECG is more sensitive than ECHO for diagnosis of ventricular aneurysm post MI

Culture is an important aspect in the main contents of bioethics.The localization of bioethics and culture are very inseparable.By studying the relationship between bioethics and culture,the author analyzed the difficulties in the development of the localization of bioethics in Chinese diversified cultural context,and proposed some ideas and recommendations on improving the localization of bioethics in China from a cultural perspective.

Objective: To evaluate shaping ability of nickel-titanium rotary instruments (Hero 642) in curved root canals. Methods:The computer assistant measure system of simulated root canal was used for quantitative analysis of root shape in eight simulated root canals during nickel-titanium rotary instruments (Hero 642) shaping process. Results:Nickel-titanium rotary instruments (Hero 642) could keep the curved angles in simulated canals very well. The cutting of Hero 642 in curved root canals was balanced and there was almost no transportation of canals central lines when the simulated canals were prepared to 0.02 taper 25~# and 30~# files. Conclusion:Nickel-titanium rotary instruments (Hero 642) has good shaping ability. In preparation of highly curved canals, the master apical file (MAF) may be Hero 642 0.02 taper 30# files.