Aorta, Abdominal ; injuries ; Blood Loss, Surgical ; prevention & control ; Hemorrhage ; therapy ; Hemostasis, Surgical ; methods ; Humans ; Mesenteric Veins ; injuries ; Splenic Diseases ; therapy ; Vena Cava, Inferior ; injuries

BACKGROUND:With the development of researches on periodontal disease etiology and neuroendocrine factors, the role of neuroendocrine factors on the pathogenesis of periodontal disease and tissue restoration has become a hot spot.
OBJECTIVE:To summarize the biological effect of neuropeptides on periodontal tissues and the changes of periodontal tissue after nerve injury.
METHODS:A computer-based search of articles was performed in CNKI, Wanfang and PubMed databases using the key words ofneuropeptides, calcitonin gene related peptide, substance P, vasoactive intestinal peptide, neuropeptide Y and periodontal tissuein Chinese and English. The included articles focused on the effect of various neuropeptides on periodontal tissue.
RESULTS AND CONCLUSION:Calcitonin gene related peptide, substance P, vasoactive intestinal peptide, and neuropeptide Y positive nerve fibers are widely distributed in periodontal support tissues. When sensory nerves are damaged, the target cel s in periodontal tissues are influenced due to changes of nerve fibers distribution and neuropeptide release. They also play a role in the reconstruction of alveolar bone, immune function of periodontal ligament and periodontal tissue. This evidence shows that neuroendocrine factors are closely linked with periodontal tissue. However, these studies concentrated in the animal models for orthodontic and fracture, the effect of the neurotransmitters such as neuropeptides on periodontal disease and the mechanisms need further exploration.

Objective To evaluate the clinical efifcacy of Matrine injection on malignant cancer pain treatment. Methods 100 patients in Central Hospital of Huanggang from February 2010 to April 2013 were randomly divided into two groups(control group and research group) for retrospective study(n=50). Based on the conventional treatment, control group were received three-step analgesic ladder, and research group were treated with matrine injections. The pain scores and Karn of sky scores were evaluated and compared before and after treatment in each group, and were compared between two groups. Results The pain score and Karn of sky score in research group after treatment were signiifcantly better than control group(P<0.05). Conclusion Matrine injection could relief pain level and improve patients' quality of life of malignant cancer patient.

Metebonomics is a branch of system biology,which,based on group index analysis, aims to analyze low molecular weight metabolites of the body both qualitatively and quantitatively through high flux and data processing.In recent years,the application of metabonomics to research of cardio-vascular pharmacology has been attracing more attention.This paper,by researching do mestic and overseas literature,summarizes the applications of metabonomics in the prevention and treatment of co mmon cardiovascular diseases such as hypertension,hyperlipide mia,atherosclerosis and myocardial ische mia.Related pharmacological researches targeted at patients,ani mal models and cell models clas-sified.Evidence for evaluation of effects and mechanis ms of drugs is provided by analyzing the patterns of change of metabolites in the progression of diseases.Finally,meaningful biomarkers are proposed.

Objective:To investigate the effects of oxaliplatin (OXA) and teniposide (VM-26) on proliferation and apoptosis of gastric cancer cell line BGC-823.Methods:MTT assay was used to examine the inhibition rate of cell growth when cells were treated at various concentrations of OXA and VM-26 alone or in combination. The apoptosis was examined by flow cytometry (FCM). The protein expression of cell apoptosis associated proteins caspase-9 and livin were examined by immunocytochemistry. Results:OXA or VM-26 effectively inhibited the growth of BGC-823 gastric cancer cells in a concentration-dependent manner at certain range of concentrations. The inhibitory rate of combined treatment with OXA and VM-26 was significantly higher than that of OXA or VM-26 alone (P<0.05). The value of combination index (CI) was 0.46. The rate of apoptosis cells induced by 1.25 μg/mL OXA was 6.13%, 13.86% and 21.48% at 12, 24 and 48 h. The apoptosis rate induced by 0.625 μg/mL VM-26 was 4.60%, 10.72% and 17.07% for 12, 24 and 48 h. In combined treatment group the apoptosis rate was 11.73%, 24.14% and 44.75% at 12, 24 and 48 h, respectively. The difference was significant between combined treatment group and single drug treatment group (P<0.05). Immunnocytochemical analysis showed that the expression of caspase-9 protein was up-regulated after being exposed to OXA (1.250 μg/mL) or OXA plus VM-26 (0.750 μg/mL), while the expression rate of livin protein was down-regulated. There were significant differences among different treatment groups as well as between treatment groups and control group (P<0.05).Conclusion:OXA combined with VM-26 has synergistic effects in inhibiting the growth and inducing apoptosis of gastric cancer BGC-823 cells.

Objective To investigate the relationship between the expressions of maspin gene in colorectal membrane,and their roles in the occurrence,progression,metastasis and prognosis of colorectal adenocarcinoma.Methods Immunohistochemistry technique was used to detect the expression of maspin in tissues of normal controls,adenoma,colorectal adenocarcinoma primary tumor,liver metastasis,lymph-node metastasis respectively.Results The expressions of maspin in normal controls,adenoma and colorectal adenocarcinoma primary tumor are down-regulated successively; the expression of maspin in colorectal adenocarcinoma with metastasis is lower than that in colorectal adenocarcinoma without metastasis(P＜0.05) ; the down-regulation of maspin expression is significantly correlated with the depth of invasion,differentiation,liver metastasis and lymph node metastasis of colorectal adenocarcinoma(P＜0.01,P＜0.05,P＜0.01,P＜0.05).Conclusion The down-regulation of expression of maspin probably plays an important role in the occurrence,progression and metastasis of colorectal adenocarcinoma.maspin is probable a predictive index for reflecting the metastasis of colorectal adenocarcinoma and provide a clue for early diagnosis of metastasis of colorectal adenocarcinoma.

BACKGROUND:With the continuous development of minimal y invasive techniques and computer navigation assistive technology, they have powerful attraction and development prospects in theory and practice. But there are controversies and misunderstandings in the application and efficacy of total knee arthroplasty.
OBJECTIVE:To summarize and evaluate the definition indications, contraindications and complications of minimal y invasive total knee arthroplasty, as wel as the advantages and disadvantages after replacement and the application of computer navigation assistive technology.
METHODS:A computer-based retrieve was performed in CNKI database and PubMed database for the articles on minimal y invasive total knee arthroplasty from 2003 to 2013. The key words of“minimal y invasive total knee arthroplasty, minimal incision total knee arthroplasty, computer navigated total knee arthroplasty, computer assisted total knee arthroplasty”in English and Chinese were put in the title and abstract. The articles on the minimal y invasive total knee arthroplasty and computer assisted total knee arthroplasty were included, and the old and repetitive articles were eliminated. Final y, 44 articles were included for review according to the inclusion criteria.
RESULTS AND CONCLUSION:Knee joint diseases can seriously affect the life quality of the patients. Minimal y invasive total knee arthroplasty is a newly developed technique in recent ten years, which can provide a new choice for the patients. Studies have shown that minimal y invasive total knee arthroplasty has the advantages of reducing postoperative pain, decreasing the direct cost and fastening the recovery. The computer navigated total knee arthroplasty can make the force line more precise after replacement. But there exist many problems according to the reports at home and abroad, especial y the life and prosthesis and time for revision, those are the fatal factors for the patients. But minimal y invasive surgery is the developing trend of orthopedic surgery.

Chronic myeloid leukemia is a kind of malignant cloning hyperplastic disease of hematopoietic stem cell .The treatments based on molecular biological and immunological techniques will become the new therapies .Gene silence can improve the effects of molecular targeted therapeutic drugs through two ways: one is the target mRNA can be digested by siRNA and the other is target gene lose the stability and reduce the generation of protein mediated by miRNA .Adoptive cel-lular immunotherapy is a treatment method through injecting immunocompetent cells such as CIK , NK, etc into the body of cancerous person .This can improve the immunity of body and the effects of molecular targeted therapeutic drugs .The fur-ther study about basic theory , molecular mechanism and clinical effects will be continued .

Bmi-1 is a core member of the polycomb group genes.As a proto-oncogene,Bmi-1 plays an important role in cell self-renewal,proliferation and apoptosis.Several studies have shown that Bmi-1 is highly expressed in some head and neck malignant tumors,such as nasopharyngeal cancer and laryngeal carcinoma.Furthermore,the expression level of Bmi-1 is closely related to the occurrence,development,incursion and prognosis of tumor.Bmi-1 is expected to become a novel tumor molecular marker,and provides a new direction for the treatment of the head and neck malignant tumor.

Stroke is a type of neurological disease with high morbidity,disability,and mortality.It has become a global public health problem.At present,the stroke burden in countries around the world has shown an upward trend.This article reviews the extent of population health hazards caused by stroke,as well as the evaluation of economic burden of the disease.