OBJECTIVETo observe the dynamic changes of membrane-bound proton-translocating ATPase (F-ATPase) in the development of dental caries, the expression of Streptococcus mutans F-ATPase under different pH concentrations and during the development of dental caries is analyzed.

METHODSStreptococcus mutans cultured under different pH (pH4.0-7.0) concentrations and containing 5% glucose and no glucose containing BHI were collected. RNA was extracted. Subsequently, F-ATPase gene was detected using real-time polymerase chain reaction. Male Wistar rats were divided randomly into caries group and control group. The rats in the caries group were fed caries feed and 5% glucose water, whereas those of control group were fed normal feed. Total RNA was extracted from plaque samples, which were collected from rats' oral cavity every two weeks. F-ATPase gene was detected by real-time PCR. In the 11th week, the upper and lower jaw bone specimens of rats were taken, and molar caries damage assessed.

RESULTSThe expression of F-ATPase in the caries group was higher than that in the control group (P<0.05). In addition, the gene was expressed highest in pH5.0 and the lowest in pH4.0 (P<0.05). 2) The expression of F-ATPase progressively increased during the caries development in both groups; expression in the caries group was higher than that in control group (P<0.05).

CONCLUSIONAcid-resisting viru-lence factor F-ATPase is related closely with the incidence and development of dental caries.

Adenosine Triphosphatases ; metabolism ; Animals ; Dental Caries ; metabolism ; microbiology ; Dental Plaque ; microbiology ; Male ; Protons ; Random Allocation ; Rats ; Rats, Wistar ; Real-Time Polymerase Chain Reaction ; Streptococcus mutans ; drug effects ; genetics ; Virulence Factors

Hirschsprung's disease (HD) is a conginental disorder of enteric nervous system (ENS) and is associated with the abnormal migration of gut neural cerst cell (GNCC).Recent studies showed that the regulation of this migration is a complex gene network.By the development of epigenetic and stem cells,this network is becoming much more clear.Transplantation of the modified gut neural crest stem cells/gut neural crest precursors(GNCSC/GNCP) acquired from the HD bowl is becoming the potential therapy of HD.This paper summerizes the recent advances and is to promote better understanding the pathogenesis of HD.

This article consults the literature on acupuncture-moxibustion treatment for the non-spastic stage of cerebral stroke published from 2001 to 2014, makes a summary and analysis from meridians, point selection, acupuncture reinforcing-reducing techniques and electoacupuncture application and points out some problems such as valuing acupuncture and neglecting moxibustion, valuing acupuncture and neglecting medicine, and neglecting point selection based on syndrome differentiation in acupuncture-moxibustion treatment for the non-spastic stage of cerebral stroke. The article also objectively assesses the position of acupuncture-moxibustion treatment, puts forward relevant solving ideas and emphasizes the importance of acupuncture manipulation and the necessity for combining Chinese herbal medicine to treat the non-spastic stage of cerebral stroke.

Objective To investigate effects of sinusitis mixture on functional endoscopic sinus after surgery .Methods According random number table,36 cases of chronic nasosinusitis patients were divided into the treatment group and control group,the two groups were routinely 2%NaCl saline nasal irrigation,use of nasal corticosteroids and mucous discharging agent mixture used in postoperative patients with sinusitis in the treatment group oral therapy. Results The treatment group 100%,total efficiency in the control group (89%) (χ2 =4.36,P<0.05),there was a statistically significant difference.Conclusion Sinusitis mixture has significant efficacy on the recovery of patients afterfunctional endoscopic sinus surgery.

To eliminate cancer hazards to human health,the development of the therapeutic cancer vaccine has been given more and more attention.By contrast of tradition therapy,cancer immunotherapy take the advantage of being able to achieve target therapy and take modest harm to organism.Here,we first idiscussed the mechanisms of cancer immune escape,and reviewed the design principles of therapeutic cancer vaccine,as well as the latest research progress of therapeutic cancer vaccine and its application.

As the completion of human genome project, application of the knowledge and techniques developed during thpast decade to the medical practice become more and more important and gain more and more attention. It is worthwhile to look back to the step prints of the development of medical genetics in China.1 The sprout of genetics in ChinaIt should be mentioned fromthe very beginning of the birth of the genetics in China to understand the historymedical genetics in China. In 1922, C.CHEN (Zhen CHEN) opened genetic courses in National Southeast Unversity after he returned from the T.H.MORGAN’s lab at Columbia University. He used Goldfish as a model fogenetic study. The Chinese scholars started their work on human genetics by survey the frequency of ABO bloogroup in the Chinese. Their result was published in1918. This was the first data of gene frequency of Chinese population, followed by reports on the inherited disease in Chinese on the frequency of color blindness in 1937. I1948, T. C. HSU described the ability to fold up the tip of the tongue as a recessive trait (MIM189300)[1,2]C.C.LI (Ching Chun LI) wrote a book,Introduction to Population Genetics, which became a wellknown book ithe world and from which a whole generation of geneticists benefited.

Objective To compare the efficacy and safety of duloxetine and gabapentin in the treatment of diabetic peripheral neuritis .Methods 135 patients with diabetic peripheral neuritis were divided into the observation group(71 cases) and the control group (64 cases) according to random number table method ,and all patients received hypoglycemic therapy based on diabetes treatment principle .Furthermore,the observation group received oral duloxe-tine,while the control group was given oral gabapentin .The pain scores of all patients were evaluated using visual ana-log scale(VAS) and medical outcomes study (SF-36),and the adverse reactions were recorded during treatment . Results (1) Compared with before treatment ,both VAS scores and SF-36 scores in observation group and control group declined significantly at the end of 1st,2nd,4th week after treatment(all P<0.05),while there were no statis-tically significant differences in VAS scores and SF-36 scores between the two groups at the same time point ( all P>0.05).(2)The incidence rates of dry mouth ,drowsiness,fatigue and dizziness in observation group were significantly lower than those in control group (18.31% vs.34.38%,4.23% vs.26.56%,0.00% vs.23.44%,0.00% vs. 15.63%,χ2 =4.059,13.308,4.059,4.059,all P<0.05),while there were no statistically significant differences in incidence rates of nausea ,diarrhea and flustered between the two groups (all P<0.05).Conclusion The duloxetine and gabapentin in the treatment of diabetic peripheral neuritis have similar efficacy , but the duloxetine has better security.

AIM: To detect the expression of ALEX 1 in the breast cancer tissues in order to verify whether ALEX1 has correlation with clinical pathological features in breast cancer .METHODS:Real-time PCR and immmunohis-tochemistry were applied to detect the expression of ALEX 1 at mRNA and protein levels in the breast tissues .The statistical analysis were performed for determining the correlation with the level of ALEX 1 and the clinical pathological features in breast cancer .RESULTS:The protein levels of ALEX1 in the breast cancer tissues were lower than that in the non-breast cancer tissues (P<0.01).The expression of ALEX1 had correlations with pathological grade , clinical stage, molecular type (P<0.05) but had no correlation with the patients ’ age, tumor size and tumor types in breast cancer .Furthermore, the result of real-time PCR showed that mRNA expression of ALEX 1 was also significantly reduced in the breast cancer tis-sues (P<0.01).CONCLUSION:The expression of ALEX1 in the breast cancer tissues is lower than that in non-breast cancer tissues .The pathological grade and clinical stage in breast cancer are negatively correlated with the expression of ALEX1.

LL-37 is the only member of the cathelicidin family known to exist in human acting as an endogenous antibiotic.After removal of the highly conserved N-termini of precursor hCAP18,a 15 000 C-terminal fragment called LL-37 bearing all of the known biological acivity hitherto is liberated by proteinase.LL-37 is active against both Gram-positive and Gram-negtive bacteria.Its antibacterial activity depends on the formation of ?-helical conformation.The cytotoxic activity of LL-37 is inhibited by apolipoprotein A-Ⅰ(apoA-Ⅰ) in the plasma,thereby it can function as a scavenger of LL-37 and prevent host cell damage.In addition to its microbicidal activity,LL-37 has several other functions such as chemotactic,angiogenic action and the ability to bind and neutralize the endotoxin.

Objective To evaluate the effects of mechanical stretch preconditioning on pathological stretch-induced damage to type Ⅱ alveolar epithelial cells.Methods A549 cells cultured in vitro were randomly divided into 6 groups (n =5 each)using a random number table:control group (Ⅰ group),pathological stretch group (Ⅱ group) and different time mechanical stretch preconditioning + pathological stretch group (group Ⅲ-Ⅵ).A549 cells were exposed to 20％ cyclic stretch at 0.3 Hz for 6 h in Ⅱ group.In Ⅲ-Ⅵ groups,A549 cells were exposed to 5％ cyclic stretch at 0.3 Hz for 15,30,60 and 120 min,respectively,and then exposed to 20％ cyclic stretch at 0.3 Hz for 6 h.After the end of pathological stretch,the apoptosis in cells was detected using flow cytometry and was calculated.F-actin expression was determined using flow cytometry.Results Compared with group Ⅰ,the apoptosis rate was significantly increased,and the expression of F-actin was up-regulated in Ⅱ group.Compared with group Ⅱ,the apoptosis rate was significantly increased in groups Ⅳ-Ⅵ,and the expression of F-actin was up-regulated in groups Ⅴ and Ⅵ.Conclusion Preconditioning with mechanical stretch (30-120 min) can mitigate pathological stretch-induced damage to type Ⅱ alveolar epithelial cells.