OBJECTIVE:To observe therapeutic efficacy of citalopram in the treatment of post-stroke depression. METHODS:126 post-stroke depression patient were selected and randomly divided into observation group and control group with 63 cases in each group. Control group was given routine treatment of nervous system drugs;observation group was given citalopram,20 mg, qd,after breakfast,on the basis of control group.They were given relevant medicine for consecutive 2 months. The mental status, the levels of plasma substance P (SP),plasma adrenocorticotropic hormone releasing hormone (CRH) plasma neuropeptide Y (NPY),rehabilitation compliance were observed in 2 groups before and after treatment. RESULTS:After treatment,HAMD and NIHSS score of observation group were decreased obviously,and lower than control group, with statistical significance (P<0.05);MMSE score of observation group increased significantly,and higher than control group,with statistical significance(P<0.05). After treatment,SP and CRH levels of observation group were decreased significantly,and lower than control group;NPY level increased significantly and higher than control group,with statistical significance(P<0.05). After treatment,complete com-pliance of observation group (71.43%) was obviously higher than that of control group (49.21%),with statistical significance (P<0.05). The maximum effect of observation group was significantly better than that of control group,with statistical signifi-cance(P<0.05). CONCLUSIONS:Citalopram in the treatment of post-stroke depression can significantly improve the patients' de-pression,nervous function and rehabilitation compliance of patient,showing significant therapeutic efficacy.

Cancer stem cells (CSC) have been defined as an unique subpopulation in tumors that possess the ability to initiate tumor growth and sustain tumor self-renewal. The tumor stem cell model proposes that tumor stem cells are the origin of most tumors. Accompanying with further extension in the investigation of cancer stem cells, the effects of niche that serves as'their microenvironment in tissue on the functions of cancer stem cells attract more and more attention, which plays important roles in maintaining the characteristics of cancer stem cells. It is essential to understand how cancer stem cells interact with their microenvironment. Although the evidence has been provided to support the existence of CSCs in various solid tumors, the identity of gastric CSCs has not been reported. In this review, we will examine gastric cancer stem cell niches and their impact on stem cell biology. The research on gastric cancer stem cell will provide new approaches for improved understanding of gastric cancer biology and gastric cancer treatment.

Bibliometrical method was used in this article to analyze the publications in a hospital from 2004 to 2013.Results show that the number of publications increased during 8 years,and the quality has been improved consistently.However,the rate of clinician participation in research and the present of core authors were still on the lower side.Besides,the age structure of core authors is steady and lack of growth.Based on this analysis,we summarized the past experience and explored the strategies of research management.

Metabolic syndrome (MS) is a complex polygenic inheritance disease, caused by the accumulative effect of multiple genes and some environmental factors. Insulin resistance is the pathophysiologic foundation of MS. From the viewpoint of TCM, phlegm is the basic pathological factor of MS. In different periods and different symptoms of the disease, phlegm can be found during the whole disease course. Genes as an important component of the innate factors, play an important role in the formation of phlegm syndrome. Researches on correlation between susceptibility genes and phlegm syndrome in MS are important to enrich the connotation of phlegm syndrome and improve the level of diagnosis and treatment for this disease.

Objective To observe the effects of Er-YAG and surgical resection in the treatment of oral cav-ity mucous cyst. Methods A total of 63 patients with oral cavity mucous cyst ,selected in our hospital from March 2013 to May 2014,were divided into control group(n=31)and experimental group(n=32)based on dif-ferent therapies. The oral cavity mucous cysts in the experimental group and the control group were treated with Er-YAG and surgical resection ,respectively. The two groups were compared in terms of therapeutic effect. Results The cure rate in the two groups had no significant difference (P>0.05). The operation time in the experiment group was significantly shorter than the control group(P<0.05). In the experiment group,the incidence of intra-operative blood loss,postoperative edema and postoperative scarring were significantly lower than those of the con-trol group(P<0.05),but the two-year recurrence rate in the two groups had no significant difference(P>0.05). The SAS scores had no significant difference between the two groups at 1 week after operation (P > 0.05),but they were significantly higher in the experiment group than in the control group before operation and 3 days after op-eration(P < 0.05). In HAD emotional determination,the numbers of patients who had the manifestation of anxi-ety and depression in the experiment group were smaller than control group before operation and 3 days after opera-tion(P<0.05),but there was no significantly difference between the two groups at 1 week after operation(P >0.05). Conclusions The curative effect of Er-YAG in the treatment of oral mucous cysts is close to that by the conventional surgery,but the former is advantageous for easier operation,less postoperative complications and alle-viation of patientspsychology pressure,worthy of clinical spreading.

Objective To evaluate the short-term clinical results of total hip arthroplasty(THA) through a direct anterior minimally invasive approach aided by lower extremity tractor for hip diseases.Methods Between August 2013 and August 2015,115 patients (138 hips) were admitted for hip diseases.They were 71 men and 44 women,aged from 28 to 77 years (average,59.4 years).Their body mass indexes ranged from 19.7 kg/m2 to 28.9 kg/m2,averaging 24.7 kg/m2.Their hip diseases included avascular necrosis of the femoral head in 56 cases (with an average disease course of 15.7 months),hip osteoarthritis in 35 cases (with an average disease course of 12.6 months),hip dysplasia in 14 cases (with an average disease course of 2.8 years) and inflammatory arthritis in 10 cases (with an average disease course of 6.8 years).All the patients were treated by primary THA through a direct anterior minimally invasive approach and assistance of lower extremity tractor.Results All the 115 patients (138 hips) were followed up for an average of 31.6 months (from 12 to 40 months).Their average length of incision was 9.4 cm,average operative time 115.9 min,average blood loss 210.2 mL,and average hospital stay 7.5 days.Their postoperative imaging results were as follows:the angles of acetabular abduction averaged 41.6° (from 37° to 56°) and the angles of acetabular anteversion 18.1° (from 11° to 23°).Their average Harris hip score one year postoperatively was 91.4 points (from 80 to 96 points).Split fracture of the proximal femur happened in one patient (one hip) (0.7％) intraoperatively;injury to lateral femoral cutaneous never was observed in 6 patients (7 hips) (5.1％).Complications like deep infection,hip dislocation,deep vein thrombosis or prosthetic loosening were not observed during follow-ups.Conclusions THA with assistance of lower extremity tractor through a direct anterior minimally invasive approach can lead to fine short-term clinical results,including quick recovery of hip function and decreased intraoperative and postoperative complications.

Objective To evaluate the value of uhrasonography in diagnosing the occult dorsal carpal ganglion. Methods Sixteen patients with chronic dorsal wrist pain received ultrasound examination. Eleven ofthem were treated with surgical therapy. And they were followed up for 1-8 months. Results The ultrasound characteristics of oecuh dorsal carpal ganglion were depicted as follows;①The dorsal cortical bone of scaphoid and lunate was continued and without damage. ② An oval anecho lesion with clear boundany, smooth ganglion wall, lateral shandowing and posterior enhancement could be detected in the dorsal part of scapholunate joint. ③Color Doppler flow imaging showed there was not obvious blood flow signals inside and around the ganglion. Conclusions Uhrasonography could be acted as a routine examination technique after the X-ray examintion in the diagnosis of the the occult dorsal carpal ganglion.

Objective To investigate the effects of acupuncture on morphology and apoptosis related gene expression of lacrimal gland in dry eye model rabbit,and explore the possible mechanism of acupuncture on dry eye.Methods Eighteen healthy New Zealand white rabbits were randomly divided into three groups:blank group,model group,acupuncture group,6 rabbits in each group.Blank group did not accept any treatment.Model group and acupuncture group were treated with subcutaneous injections of scopolamine hydrobromide,4 times a day with the dose of 2.0 mg · kg-1,when the acupuncture group was also treated with acupuncture,once a day for fourteen days.Three groups were examined Schirmer Ⅰ test (S Ⅰt) at different stages,and morphological changes of lacrimal gland under light and electron microscope were observed.The immunohistochemical method was used to detect the protein levels of apoptosis related gene in lacrimal gland of all rabbits.Results Between the acupuncture group and model group,the S Ⅰt was significantly different on day 4,7,14 (all P ＜ 0.05).At the same time,compared with that before experiment,S Ⅰt had no significant difference on day 14 in acupuncture group (P ＞ 0.05).Optical and electron microscope showed the lacrimal gland epithelial cells of acupuncture group closely arranged with abundant cytoplasm,closed to that of the blank group.Meanwhile,the number of lacrimal epithelium cells with Bax,Fas and FasL positive expression was significantly less than that in the model group (all P ＜ 0.05).But when it compared with blank group,there was no significant difference of Bax/Bcl-2 and Fas/FasL positive expression in acupuncture group (all P ＞ 0.05).Conclusion Acupuncture can restrain the apoptosis and gland atrophy of lacrimal gland tissue of dry eye rabbit,and promote metabolism,thereby increase the tear secretion.

BACKGROUND:Sustained-release carriers of insulin shaped as microspheres made of different biomaterials have become an issue of concern. OBJECTIVE:To summarize the carrier materials and methods to prepare sustained-release microspheres of insulin. METHODS:Wanfang and PubMed databases were retrieved by computer for articles related to sustained-release carriers of insulin published from 1997 to 2015. The search terms were“insulin, control ed-release carrier, biomaterials”in Chinese and English, respectively. RESULTS AND CONCLUSION:Natural biodegradable polymer materials are preferred to prepare sustained-release microspheres of insulin, including gelatin, alginate, chitosan and its derivatives. These natural materials have good biocompatibility, degradability, film-forming and microsphere-forming abilities. Synthetic biodegradable polymer materials as carrier materials can promote drug stability and effective utilization, and realize targeted drug delivery. According to different physicochemical properties of materials, sustained-release carriers of insulin that meet different requirements can be prepared using emulsion-chemical crosslinking, spray drying and solvent evaporation methods. This review provides new insight into the development of stable drug carriers.

0.1 mV. The rabbits were randomly divided into 4 equal groups of 12 animals in each group: Ⅰ control group in which normal saline 0.2-0.3 ml?kg-1 was injected through epidural catheter; Ⅱ epidural group in which 1% lidocaine 2-3 mg?kg-1 was injected epidurally; Ⅲ propofol group in which propofol was infused iv at 20 mg ? kg-1 ?h-1 for 30 min; Ⅳ combined epidural-propofol group in which epidural 1% lidocaine and propofol iv were both administered. Blood samples were taken before and 4 h, 8 h after ligation for determination of serum concentrations of myocardial troponin I (cTn I ) and MDA and serum SOD activity.The myocardial tissue was taken from the infarct area for determination of the apoptotic myocyte count (TUNEL) and p53 and Bcl-2 protein expressions (LSAB) . Results In control group serum cTn I and MDA increased and SOD decreased significantly after ligation of LAD. Thoracic epidural anesthesia, propofol iv infusion and especially the combination of them suppressed the increase in serum cTn I and MDA and decrease in serum SOD induced by ligation of LAD.In epidural-propofol group and propofol group the apoptotic myocytes count and p53 protein expression significantly decreased, the Bcl-2 protein expression increased as compared with those in control group. Conclusion Thoracic epidural anesthesia combined with propofol can significantly protect myocardium from injury induced by myocardial infarction. This action is mediated by the depression of myocyte apoptosis and the generation of oxygen-derived free radicals.