OBJECTIVE:To observe therapeutic efficacy of citalopram in the treatment of post-stroke depression. METHODS:126 post-stroke depression patient were selected and randomly divided into observation group and control group with 63 cases in each group. Control group was given routine treatment of nervous system drugs;observation group was given citalopram,20 mg, qd,after breakfast,on the basis of control group.They were given relevant medicine for consecutive 2 months. The mental status, the levels of plasma substance P (SP),plasma adrenocorticotropic hormone releasing hormone (CRH) plasma neuropeptide Y (NPY),rehabilitation compliance were observed in 2 groups before and after treatment. RESULTS:After treatment,HAMD and NIHSS score of observation group were decreased obviously,and lower than control group, with statistical significance (P<0.05);MMSE score of observation group increased significantly,and higher than control group,with statistical significance(P<0.05). After treatment,SP and CRH levels of observation group were decreased significantly,and lower than control group;NPY level increased significantly and higher than control group,with statistical significance(P<0.05). After treatment,complete com-pliance of observation group (71.43%) was obviously higher than that of control group (49.21%),with statistical significance (P<0.05). The maximum effect of observation group was significantly better than that of control group,with statistical signifi-cance(P<0.05). CONCLUSIONS:Citalopram in the treatment of post-stroke depression can significantly improve the patients' de-pression,nervous function and rehabilitation compliance of patient,showing significant therapeutic efficacy.

Cancer stem cells (CSC) have been defined as an unique subpopulation in tumors that possess the ability to initiate tumor growth and sustain tumor self-renewal. The tumor stem cell model proposes that tumor stem cells are the origin of most tumors. Accompanying with further extension in the investigation of cancer stem cells, the effects of niche that serves as'their microenvironment in tissue on the functions of cancer stem cells attract more and more attention, which plays important roles in maintaining the characteristics of cancer stem cells. It is essential to understand how cancer stem cells interact with their microenvironment. Although the evidence has been provided to support the existence of CSCs in various solid tumors, the identity of gastric CSCs has not been reported. In this review, we will examine gastric cancer stem cell niches and their impact on stem cell biology. The research on gastric cancer stem cell will provide new approaches for improved understanding of gastric cancer biology and gastric cancer treatment.

Objective To investigate the effects of acupuncture on morphology and apoptosis related gene expression of lacrimal gland in dry eye model rabbit,and explore the possible mechanism of acupuncture on dry eye.Methods Eighteen healthy New Zealand white rabbits were randomly divided into three groups:blank group,model group,acupuncture group,6 rabbits in each group.Blank group did not accept any treatment.Model group and acupuncture group were treated with subcutaneous injections of scopolamine hydrobromide,4 times a day with the dose of 2.0 mg · kg-1,when the acupuncture group was also treated with acupuncture,once a day for fourteen days.Three groups were examined Schirmer Ⅰ test (S Ⅰt) at different stages,and morphological changes of lacrimal gland under light and electron microscope were observed.The immunohistochemical method was used to detect the protein levels of apoptosis related gene in lacrimal gland of all rabbits.Results Between the acupuncture group and model group,the S Ⅰt was significantly different on day 4,7,14 (all P ＜ 0.05).At the same time,compared with that before experiment,S Ⅰt had no significant difference on day 14 in acupuncture group (P ＞ 0.05).Optical and electron microscope showed the lacrimal gland epithelial cells of acupuncture group closely arranged with abundant cytoplasm,closed to that of the blank group.Meanwhile,the number of lacrimal epithelium cells with Bax,Fas and FasL positive expression was significantly less than that in the model group (all P ＜ 0.05).But when it compared with blank group,there was no significant difference of Bax/Bcl-2 and Fas/FasL positive expression in acupuncture group (all P ＞ 0.05).Conclusion Acupuncture can restrain the apoptosis and gland atrophy of lacrimal gland tissue of dry eye rabbit,and promote metabolism,thereby increase the tear secretion.

Objective To evaluate the efficacy of thymosin ɑ1 combined with arsenic trioxide in the treatment of advanced primary liver cancer (PLC).Methods A total of 59 patients with advanced PLC who were admitted to Jiangyan Hospital of Traditional Chinese Medicine from July 201 1 to July 2014 were divided into two groups:control group and combination therapy group.The two groups were given symptom-atic and supportive treatment to protect the liver.In addition,29 cases in the control group were treated with arsenic trioxide,while 30 cases in the combination therapy group were administrated with thymosin ɑ1 combined with arsenic trioxide.Comparison of continuous data be-tween the two groups was made by independent-samples t test,and comparison of categorical data was made by chi-square test.Results There was a significant difference in clinical benefit rate between the control group and the combination therapy group (37.9% vs 66.7%,χ2 =4.88,P<0.05).The combination therapy group had significantly higher improvement rate of the quality of life (63.3%vs 37.9%,χ2 =3.81,P<0.05)and the pain remission rate (76.7%vs 44.8%,χ2 =6.28,P<0.05)than the control group.The levels of alpha fetal protein,carcinoembryonic antigen,carbohydrate antigen 199,and gamma glutamyl transpeptidase decreased in both groups,and the combination therapy group had significantly higher decreases in these indices than the control group (P<0.05).The combination thera-py group had significantly increased percentages of CD3 +and CD4 +T cells and CD4 +/CD8 +ratio (P<0.05)and a significantly reduced percentage of CD8 +T cells (P<0.01)after treatment.However,there was no significant difference in toxic or side effects between the two groups.Conclusion For patients with advanced PLC,the combination of thymosin ɑ1 and arsenic trioxide can improve the quality of life and the immune function of patients,and no obvious toxic or side effects are found.So it holds promise for clinical application.

Objective To investigate the effect and safety of patients with diclofenac sodium lidocaine analgesia after endoscopic opera-tion. Methods One hundred patients with the implementation of nasal endoscopic treatment in our hospital from August 2012 to June 2014 were randomly divided into observation group and control group with 50 cases in each group. The observation group were injected the diclofe-nac sodium lidocaine,which compared with the control group given fentanyl through a vein for postoperative analgesia with analgesia effect and safety. Results The differences of HR,SBP,DBP,MAP indicators were not significant between and within groups (P>0. 05) with immedi-ately after operation, postoperative 24 h,48 h after surgery. There was no significant difference on the VAS score between the inmmediately after the operation process and compared to the control group (P>0. 05). The observation group with the immediate postoperative VAS score decreased significantly compared with that of the control group after 1 h,4 h,8 h,16 h,24 h,48 h of the surgery,while the observation group VAS scores were significantly lower than that of the control group (P<0. 05). The observation group in analgesia occurred in 6 cases of ad-verse reactions ( 12%) was significantly lower than that of 16 cases of the control group ( 32%) , and the difference was significant (P<0. 05). Conclusion Better effect and low incidence rate of adverse reaction is showed that diclofenac sodium lidocaine analgesia of patients with nasal endoscopic operation.

Bibliometrical method was used in this article to analyze the publications in a hospital from 2004 to 2013.Results show that the number of publications increased during 8 years,and the quality has been improved consistently.However,the rate of clinician participation in research and the present of core authors were still on the lower side.Besides,the age structure of core authors is steady and lack of growth.Based on this analysis,we summarized the past experience and explored the strategies of research management.

BACKGROUND:Sustained-release carriers of insulin shaped as microspheres made of different biomaterials have become an issue of concern. OBJECTIVE:To summarize the carrier materials and methods to prepare sustained-release microspheres of insulin. METHODS:Wanfang and PubMed databases were retrieved by computer for articles related to sustained-release carriers of insulin published from 1997 to 2015. The search terms were“insulin, control ed-release carrier, biomaterials”in Chinese and English, respectively. RESULTS AND CONCLUSION:Natural biodegradable polymer materials are preferred to prepare sustained-release microspheres of insulin, including gelatin, alginate, chitosan and its derivatives. These natural materials have good biocompatibility, degradability, film-forming and microsphere-forming abilities. Synthetic biodegradable polymer materials as carrier materials can promote drug stability and effective utilization, and realize targeted drug delivery. According to different physicochemical properties of materials, sustained-release carriers of insulin that meet different requirements can be prepared using emulsion-chemical crosslinking, spray drying and solvent evaporation methods. This review provides new insight into the development of stable drug carriers.

Metabolic syndrome (MS) is a complex polygenic inheritance disease, caused by the accumulative effect of multiple genes and some environmental factors. Insulin resistance is the pathophysiologic foundation of MS. From the viewpoint of TCM, phlegm is the basic pathological factor of MS. In different periods and different symptoms of the disease, phlegm can be found during the whole disease course. Genes as an important component of the innate factors, play an important role in the formation of phlegm syndrome. Researches on correlation between susceptibility genes and phlegm syndrome in MS are important to enrich the connotation of phlegm syndrome and improve the level of diagnosis and treatment for this disease.

Cerebral vasospasm is the leading cause of disability and death in patients with spontaneous subarachnoid hemorrhage,and there is no definitive and effective treatment for it yet.Triple-H therapy is now the first choice in the treatment of cerebral vasospasm,but there are still more controversies about it. In recent years,there have been a number of studies about Triple-H therapy. This article reviews the implementation of Triple-H therapy,fluid selection,and prophylactic application.

The vessels of the medial skin flap of the leg was investigated on 50 cases of adult cadavers. Cutaneous branches of arteria tiblalis posterior distribute to the lower half of the medial surface of the leg, and the area of distribution is 134.03 cm2 in average. The muscular branches of the arteria tibialis posterior mainly distribute to the muscle flexor digitorum longus, and muscle soleus. Some attentions about taking free medial skin flap of the leg have been discussed.