1. NATURALIZING PLANTED HORSEMINT BIOLOGICAL
DENSMAA B. ; GANTOGTOKH G. ; BOLORTSETSEG KH.
Mongolian Pharmacy and Pharmacology 2015;7(2):61-64
We are still doing research on 30 kind of ephemeral and perennialgramineous, bosk and wooded in our country. Nation`s enterprise in industries importing essential oils from abroad and they are using in product of medicine, food and beauty. Whole world`s most of them still researching and using widely Mentha L (Horsemint) plant in crop production and if we naturalize this Mentha L it will show result good result in plant raw material of nature and important to our economy as well.Key words: Mentha longifolia; Menth arvensis, Biological characterIntroductionEach year resource availability of raw material is proved to be not enough and it shows in research of resource in medical plant range and also what plant shows low resourceof raw material has to provide before the plant germinate or during fl owering stage in every year. Nowadays this plant is destroying and possibility of grow again is decreasing, because of the people who has interest in profi t they are taking away a lot of rhizome roots. The result of 100 scrip in Mongolian medicine shows that we supplied 43.75% of raw material in plants are from our fl ora and 56.25% is from other country. So it shows bad affect to our economy that still using other country`s resource and getting import from them. Then we have naturalize the plants in our weather conditions and try to detect innovative biological active ingredients and do research that has explained by modern science. Mongolian medicine need phytogenic of rareplants from other foreign country, so that`s why we are still doing research on how to naturalize plants in our country.Goal: How to plant Metha L. (Horsemint) in fi eld area that in order to determine the nature this plant and next try to know purpose under the following main objectives. There are:1. To recognize the naturalizing plant division2. To identify the qualifi cation of naturalizing plant division and do research ratio with anatomical result3. Get recognition of the importance in Metha L. (Horsemint)Materials and methodsThings that involved in research (blossom) take middle leaf and in order to prepare slice for anatomy research, cut the middle of leaf, get in foam plastic sponge and cut it, blue, paint it with sapphire, put it on the glass shelf make jelly and in order to prepare slice done with 8-10 frequency. According to traditional method with the help of “Enaval” microscope research leaf’s inside anatomy and textural fi nished (Гзырян,1981; Эзау 1980; В.К.Василевская,А.А.Бутник, 1981; Гамалей, 1984). The plants leaf that involved in research anatomy picturedwith the help of “PA-4”that camera.
2.The method of cultivation of Rheum Undulatum L. seeds in the steppe Lands of Mongolia
Densmaa B ; Gantogtokh G ; Kristina Amalia
Mongolian Pharmacy and Pharmacology 2021;18(1):69-75
Abstract
Nowadays on par with the development of Western medicine, such issues as preserving veg- etation and ecological balance, supporting the expansion of the National Pharmaceutical Industry and “National Policy for the Development of Mongolian Traditional Medicine” are being actively discussed in Mongolia. Also, worldwide, the trend of using herbal medicine is growing2.
Due to the continuous drought for many years, the desertification process in most parts of Mongolia was very intense, and as a result rare endangered plants are on the verge of extinction 3, 4, 6. Therefore, there is the need to determine the amount of reserve, rationalize the use of medicinal plant, acclimate rare herbal plants, and create medicine from raw materials.
To resolve this matter, we prepared a methodological recommendation for the cultivation of Rheum undulatum L. With this method, it is possible to plant this kind of seed in the steppes of Mongolia and to use the medicinal raw materials from the roots of a 3-year-old plant.
3.The result on anatomical study of Inula Helenium
Densmaa B ; Gantogtokh G ; Khatanzorig N ; Kristina Amalia
Mongolian Pharmacy and Pharmacology 2022;20(1):72-77
Introduction:
In recent years, some species of plants that are used in traditional medicine and have high practical value have been successfully introduced in our country. It is necessary to carry out phytochemical studies of local medicinal and useful plants to produce food and biologically active food supplements. The tall coin flower (Inula Helenium L.) has been successfully cultivated in Mongolia, and the lower layer of the plant such as roots and stems are used. However, most of the times the top layer of the plant is not used and thrown away, that is why the plant must be fully utilized. Therefore, the aim of this study is to analyze the top layer of the plant and present the results of anatomical studies.
Methods:
Remove the top layer of the plant and soak it in glycerin for 24 hours. When making an incision, select a green, undamaged part of the plant and place it in the VCM-202III freezer microtome. Chloral hydrate liquid should be used for micro-preparation. In addition to implementing chloral hydrate solution, use the solutions of various concentrations of sodium alkali (NaOH) (5- 15%). Place the slice in alcian blue stain (using the cell wall staining method) for 3 minutes and wash it with distilled water. Then stain it in a drop of safranin staining (0.5–1.0% aqueous solution) for 1 minute. Rinse it twice with distilled water, add a mixture of glycerin and distilled water, cover it with a glass slide and prepare a temporary slide. Examine the prepared temporary slide with a NOVEL light microscope. Cell images of the plant’s anatomical structures are captured on a computer screen with the help of a digital camera.
Conclusion
The anatomy of the leaves, stems and flowers of the tall coin flower (Inula Helenium L.), a plant cultivated in Mongolia, has been analyzed.
4.Results of determination of salidroside content in roots and rhizomes of cultivated and natural Rhodiola rosea L
Khishigjargal B ; Lkhaasuren R ; Batdorj D ; Suvdaa T ; Gantogtokh G ; Orkhon N ; Tsetsegmaa S ; Khurelbaatar L
Mongolian Medical Sciences 2021;195(1):51-55
Introduction:
Rhodiola rosea L. (R.rosea) is a popular plant in traditional medicine of the Nordic countries, Eastern
Europe, and Asia. R.rosea plants are successfully cultivated in Mongolia. The Botanical Garden of
Medicinal Plants under the “Monos” Group started to cultivate R. rosea since May 2015.
Objective:
The aim of this research was to study the salidroside contents of R.rosea collected from Zavkhan
and Khuvsgul province, Mongolia, and cultivated in the Botanical Garden of Medicinal Plants, Drug
research Institute, Monos group.
Material and Methods:
The underground parts of wild roseroot plants were collected from April to May 2020 from Jargalant
soum, Khuvsgul province, and Nomrog soum, Zavkhan province, 3-years and 4-years-old cultivated
R.rosea gathered from the Botanical Garden of Medicinal Plants in April 2020. For comparison,
4-year-old Rhodiola grenulata (R. grenulata) was ordered from Shanxi Zhendong Genuine Medicinal
Materials Development Co., Ltd, China, and used for the study. The quantity of the salidroside
constituents of the underground parts were compared and the sourcing of roseroot raw material was
evaluated. Chemical analysis of roots and rhizome of R. Rosea namely the appearance, identification,
moisture, organic impurities, mineral impurities, residue on ignition, water-soluble extractives, fresh
weight of roots, and salidroside content were determined according to the National Pharmacopoeia of
Mongolia (NPhM) 2011. Microbiological analysis was performed in accordance with the requirements
of grade 3b specified in Annex 1 of the Order No. A / 219 of the Minister of Health dated May 30,
2017 to determine the degree of microbiological purity in medicinal products of roots and rhizome
raw materials.
Result:
The content of salidroside, the main biologically active substance of R.rosea plant, was 1.57% in
samples collected from Zavkhan province, 1.45% in samples collected from Khuvsgul province, 1.7%
in samples grown in China and 0.25% for 3-years-old samples and 1.89% for 4-years-old samples grown in the Botanical Garden of Medicinal Plants, Monos group, Mongolia. In addition, these raw
materials meet the general requirements for plant raw materials and microbiological parameters.
Conclusion
Samples of underground parts of R.rosea cultivated for 4 years in the Botanical Garden of Medicinal
Plants have the highest content (1.89%) of the salidrosde. Therefore, it is suggested that the roots
and rhizomes of R.rosea planted in the future can be standardized and used as a raw materials for
medicines.