Objective Antiaging effects of seal kidney on aged male rats have been studies. Methods: Reproduction system of male rat was damaged by CYP. After ig. seal kidney 30 days, the content of serum T, serum MDA and the activity of SOD were determined. The changes of histology of the testis were observed by microscope with the method of paraffin embedding and H-E staining. Results The contents of serum T not only in normal rat but also in reproduction system damaged rat were markedly enhanced. The function condition of leydig's cells in the testis was improved. The formation and development of spermatozoon were increased. The activity of serum SOD was remarkably raised , while the content of serum MDA was strikingly reduced. Conclusion Seal kidney has the notable antiaging effects.

0.05).⑥Complications:1 case in the prosthetic disc nucleus replacement group had migration of the device in 1 year after the surgery,but no symptom and did not need revision;1 case complained of bilateral leg pain and 2 cases developed right leg pain in the fusion group,which were treated with oral medications and resolved after the 3-month follow-up.All patients with leg symptoms had surgery at the L5S1 disc space.CONCLUSION:①Quantitative clinical outcomes following prosthetic disc nucleus replacement are at least equivalent to clinical outcomes with traditional lumbar spinal fusion.These results support earlier reports in the literature.②Prosthetic disc nucleus replacement is a safe and effective alternative to fusion for the surgical treatment of symptomatic lumbar disc herniation in properly indicated patients.

BACKGROUND: The appearances of spiral computer tomograph (CT) and three-dimensional image processing are of very importance for fully evaluating preoperative pathological changes,which represent various cervical spondylotic myelopathy (CSM) in clinic, and choosing proper clinical therapeutic occasion and methods.OBJECTIVE: To analyze the effects of imageological image and technical advantages of three-dimensional spiral CT on the preoperative evaluation and surgical plan of CSM.DESIGN: Retrospective analysis, controlled observation.SETTING: Nanjing First Hospital Affiliated to Nanjing Medical University. PARTICIPANTS:Altogether 268 patients with CSM admitted to Nanjing First Hospital from January 2002 to January 2005 were involved in this experiment. The involved patients were randomly assigned into preoperative evaluation group (n =146) and control group (n =122). The baseline materials of two groups were comparable.METHODS:Preoperative evaluation group:① CT scan and three-dimensional reconstruction:Helical scanning was conducted at transverse plane with TOSHIBA-HiSpeed/I screw machine. After scanning, reconstruction was performed finally, three-dimensional reconstruction was conducted on computer workstation (RadworkS. 1 ). Correlative data were measured and analyzed with image analysis software (ADW3.1). ② Design of three-dimensional CT-assisted operation plan: Cervical vertebral osseous anatomic landmark and the anatomic relationship of its adjacent structure were revealed stereoscopically.Individual orthopaedic location marker could be provided.Preoperative routine CT examination was performed in the control group with conventional operation method.MAIN OUTCOME MEASURES: ① The hyperostosis of vertebral body, hamular process joint and small articular process were observed. ②The degree and type of intervertebral disc protrusion were observed. ③The changes of hyperostosis before and behind the vertebral canal. Spinal decompression was observed by CT virtual endoscopy (CT-VE).RESULTS: All the 268 patients participated in the result analysis. ① In the preoperative evaluation group, 129 patients were found with intervertebral disc protrusion,109 patients with vertebral posterior marginal hyperostosis, 61 patients with hypertrophic ligamentum flavum and corrugation, 27 patients with posterior longitudinal ligament calcification,31 patients with hyperostosis at small articular process,29 patients with vertebral plate thickening and 18 patients with vertebral olisthy.The visible anatomical structures were in accord with clinical manifestations and imageological structures. Death, laryngeal nerve injury, bone graft displacement, infection and other complications were not found in all the patients. In the postoperative 6th month, X-ray image showed that all the patients had good bone graft fusion, and no titanic plate or bolt loosening or fragmentation was found. The excellent and good rate evaluated by Odom was 95.9%.② In the control group,postoperative X-ray image and CT showed that vertebral canal decompression of 24 patients was not fully, and the positions of titanic plate or bolt of 17 patients were not satisfying. The excellent and good rate evaluated by Odom (84.4%) in the control group was lower than that in the preoperative evaluation group (P＜0.05).CONCLUSION: Preoperative observation of cervical vertebral individual three-dimensional CT is helpful to exactly and generally evaluate cervical spondylotic myelopathy and assistantly make operative plan,whick makes intraoperative manipulation more exactive, safer and easier to be controlled.

Objective To evaluate the application of infrared fluoroscopic navigation guiding pedicle screwfixation of thoracic-lumbarspine.Methods The pedicle screw fixation of thoraic-lumbar spine was adopted in 32 patients,including 19 cases with thoracic-lumbarspine fractures,5 cases with protrusion of lumbar intervertebral disc or vertebral canal stenosis,8 cases with lumbar spine slippage.The timefor pedicle screwfixation and amount of bleeding were recorded.The pedicle screwposition was assessed with post-operative CTby using An-drewgrade.Results Totally148 screws were fixed in 32 cases successfully,including10 screws in T11,20 in T12,18 in L1,36 in L2,16 inL3,26 in L4,28 in L5,and 14 in S1.According to andrewgrade,165 screws were inⅠgrade(98%),2 inⅡgrade,and 1 inⅢ grade.Nonerve and spinal cord damage was observed after operation.The average time of per pedicle screwfixation was(10?2.4) min.The averageamount of bleeding was(400?52.3) ml in thoracic-Lumbar Spine fractures,(200?36.8) ml in protrusion of lumbar intervertebral disc orvertebral canal stenosis,and(300?44.6) ml in Lumbar Spine slippage.Conclusion Infrared fluoroscopic navigation can improve the pre-cision of pedicle screw fixation of thoraic-lumbar spine,reduce the amount of bleeding,and shorten the operative time.

OBJECTIVETo observe changes of thromboxane B2 (TXB2), 6-keto-prostaglandin F1 alpha(6-Keto-PGF1 alpha) and TXB2/6-Keto-PGF1 alpha (T/P) in lungs of rats drowned in hypothermic sea water and to assess their influence on the blood-gas.

METHODSRats of different groups were drowned nearly to death in hypothermic sea water and then taken out of the water rapidly, observed at room temperature, after that the following steps were taken in 5, 15, 30, 60, 240 min and 360 min groups, that were 1 ml arterial blood taken from left heart for blood-gas analysis including pH, PaO2 and PaCO2, rectal temperature observed; at last, the ratio of left dry lungs with left wet lungs was assessed, TXB2 and 6-Keto-PGF1 alpha in right lungs were examined in all above groups and dead group(14 rats dead, only 4 examined).

RESULTSThe rectal temperature[(20.13 +/- 0.48) degree C], pH(6.68 +/- 0.03), PaO2[(45.00 +/- 6.30) mm Hg)], TXB2[(97.46 +/- 17.46) ng/L] and 6-Keto-PGF1 alpha[(25.59 +/- 8.12) ng/L] dropped to the lowest point in the 5 minutes group(P < 0.01), while PaCO2[(89.18 +/- 5.10) mm Hg] reached the highest point(P < 0.01), all above items from 5 minutes group then showed a recovering tendency, but only the pH in 240 minutes and 360 minutes groups as well as TXB2 in 360 minutes group and dead group reached near the level of normal control groups (P > 0.05); T/P had a rising tendency and reached the highest point in the 360 minutes group.

CONCLUSIONSThe production and secretion of TXB2 and 6-Keto-PGF1 alpha were influenced by hypothermia, hypoxemia and acidosis, the imbalance of T/P could be one of factors influencing the improvement of blood gas index.

6-Ketoprostaglandin F1 alpha ; analysis ; Animals ; Body Temperature ; Carbon Dioxide ; blood ; Drowning ; metabolism ; Hypothermia ; metabolism ; Lung ; chemistry ; Oxygen ; blood ; Rats ; Seawater ; Thromboxane B2 ; analysis

Objective:To investigate the lower limb muscle strength characteristics in amateur runners with patellofemoral pain(PFP). Method:Thirty PFP amateur runners(20 males and 10 females)were recruited as the knee pain group,and 30 matched non-injured amateur runners were recruited as the non-knee pain group.The hip abductor strength,quadriceps strength,hamstring strength,hamstring/quadriceps ratio,and bilateral limb symmetry index were col-lected for all participants.Independent sample t-tests were used to compare the differences between the groups. Result:The hip abductor strength and bilateral limb symmetry index,quadriceps strength and bilateral limb symmetry index of male PFP amateur runners were significantly lower than those of the non-knee pain group,while the hamstring/quadriceps ratio was significantly higher than that of the non-knee pain group.The hip ab-ductor strength and bilateral limb symmetry index of female PFP amateur runners were significantly lower than those of the non-knee pain group. Conclusion:PFP amateur runners exhibit different lower limb muscle strength characteristics compared to non-knee pain runners,and the lower limb strength characteristics of male and female PFP amateur runners are not consistent.Therefore,functional and gender differences should be considered in the clinical treatment and rehabilitation training of PFP.

Objective:From the perspective of patients'emotions,to compare and study the effectiveness between experiential communication and GLTC communication of outpatient doctors,understand the main communication details that affect patients'emotions,and provide a reference for improving patient emotions and enhancing doctor-patient communication skills in the future.Methods:From July 2021 to January 2022,a pilot study of GLTC communication program in outpatient settings was conducted in a total of 24 departments from 4 tertiary comprehensive hospitals in Jiangsu Province.Descriptive analysis,independent sample t-test,multiple linear regression and other analyses were conducted on the relevant data.Results:After experiential communication in outpatient settings,patients showed improvements in dimensions of emotion such as anxiety,fatigue,confusion,and depression,but there was a decrease in patient vitality(P<0.05).After GLTC communication in outpatient settings,patients'emotions in all six dimensions improved(P<0.05).Meanwhile,the GLTC group had better emotions than the experiential group after communication(P<0.05).After training,the completion rate of relevant communication details of doctors in the GLTC group was mostly significantly higher than that in the experiential within the same unit time(P<0.05).Multiple linear regression showed that smiling,comforting language,and kind gaze were the main factors affecting patients'overall emotions.Conclusion:Experiential communication in outpatient settings has certain communication effects,but there is still a lot of room for improvement compared to GLTC communication in outpatient settings.GLTC communication in outpatient settings can improve patients'emotions,and doctors'smiles,comforting language,and other communication details need to be emphasized in outpatient settings.How to improve the execution of GLTC communication in outpatient settings plans is an important direction for future research.