AIM: To establish a method of simultaneous determining 4 components in Shengmai Injection (Radix et Rhizoma Ginseng Rubra,Radix Ophiopogonis,Fructus Schisandrae Chinensis). METHODS: Four components:ginsenoside Rg_1、ginsenoside Re、ginsenoside Rb_1 and schisandrin in Shengmai Injection were determined simultaneously by HPLC, using a waters symmetryshield TMRP_(18) column(4.6 mm?250 mm; 5.0 ?m),acetonitrile-water as a mobile phase. The detection wavelength was at 203nm. RESULTS: The relationship between the concentrations and the peak areas of ginsenoside Rg_1、ginsenoside Re、ginsenoside Rb_1 and schisandrin was linear respectively.The RSD of precision,repeatability and recovery were all less than 1%. CONCLUSION: The method is simultaneous determination for four components in Shengmai Injection,and can be applied to the quality control of Shengmai Injection.

Objective To study the chemical constituents of Phlomis medicinalis.Methods The constituents were isolated and purified by various modern chromatography techniques and the structures were elucidated by physicochemical properties and spectral analyses.Results Ten compounds were obtained and elucidated as 1-dehydroxyshanzhigenin methyl ester(Ⅰ),notohamosin A(Ⅱ),verbascoside(Ⅲ),3,4-dihydroxyphenyl ethanol(Ⅳ),succinic acid(Ⅴ),sucrose(Ⅵ),2,6-difructose(Ⅶ),butyl-?-D-fructopyanoside(Ⅷ),D-glucose(Ⅸ),and D-fructose(Ⅹ).Conclusion All the compounds are isolated from this plant for the first time,compound Ⅱ is obtained from the plants of Phlomis Linn.for the first time,and compound Ⅰ is a new one.

AIM: To study Cordyceps sinesis and its cultured mycelia by HPLC.METHODS: An HPLC established for the fingerprints of cordyceps sinensis and its cultured mycelia was performed on an Agilent C18(4.6 mm ? 25 mm,5 ?m)as column and acetonitrile-0.1% phosphoric acid solution in gradient as mobile phase.The flow rate was 0.5 mL/min,and the detection wavelength was set at 260 nm.RESULTS: There were 6 common peaks in HPLC fingerprint of Cordyceps corensis and its cultured mycelia,three of which were determined as uracil,adenosine,cordycepin.CONCLUSION: The method can be used for the study of fingerprints of cultured mycelia.

Objective To investigate the chemical constituents of Balanophora simaoensis. Methods Chromatography and spectra were used to isolate the constituents and elucidate their structures. Results Four compounds were isolated from whole plant of B. simaoensis and elucidated as methylconiferin (Ⅰ), butylconiferin (Ⅱ), 4-O-?-D-glucopyranosyl confieryl aldehyde (Ⅲ), and brevifolin (Ⅳ), respectively. Conclusion Butylconiferin is a new compound.

Objective: To study the specificity of the determination method for catechin and epicatechin in Catechu. Method: Contents of catechin and epicatechin in 7 kinds of crude drugs and 3 kinds of preparations of Catechu were determined. Results: As sampling 100 times as much as Catechu, catechin and epicatechin could be both detected in Semen Arecae, and catechin could be detected in Radix et Rhizoma Rhei, Herba Ephedrae and Radix Sanguisorbae. Conclusion:This method can be used for the quality control of Catechu and its preparations and is of specificity.

AIM: To affirm marker peaks for the fingerprint chromatography of Shengmai Injection. METHODS: LC-MS/MS method was used, with a Waters symmetryshield TM RP_ 18 column(4.6 mm?250 mm; 5.0 ?m), acetonitrile-water as a mobile phase, The detection wavelength was at 203 nm. Ion trap mass spectrum. RESULTS: Affirming marker peaks for fingerprint chromatography of Shengmai Injection and 10 marker peaks were affirmed. CONCLUSION: The method can affirm marker peaks for the fingerprint chromatography of Shengmai Injection. It is simple, accurate, and has practicality.

A multi-residue analysis method was developed for the determination of 38 kinds of pesticides in nuts (almonds, peanuts, cashew nuts and walnuts) by QuEChERS-ultra-high performance liquid chromatography-tandem mass spectrometry.The pesticide residues were extracted with acetonitrile.The extract was cleaned up with PSA, C18 and Oasis PRiME HLB, and then analyzed by UPLC-MS/MS with multiple reaction monitoring (MRM) mode.External standard method was employed to quantify.The limits of detection (LODs, S/N=3) of this method were between 0.01 and 10 μg/kg, and the limits of quantitation (LOQs, S/N=10) were between 0.05-20 μg/kg.All of the tested pesticides showed good linear relationship (r>0.991).The practical samples were determined at three spiked levels and the average recoveries were between 51.0% and 126.0%.The RSDs were less than 20%.This method was simple, sensitive and accurate, and could be used for the routine analysis of pesticide residues in nuts.

Objective To study the chemical constituents in the roots of Phlomis medicinalis.Methods The compound was isolated and repeatedly purified by macroporous resin,silica gel column chromatography,TLC,and Prep-HPLC.Its stracture was elucidated by physical and chemical properties and NMR spectra.Results One iridoid glucoside was obtained and elucidated as 8,10-dehydropulchelloside.Conclusion The compound is a new one.