1.Study of MAPKKK5 gene expression of brain tuissur in patients with cryptogenic refractory temporal lobe epilepsy
Gang ZUO ; Zhen HONG ; Wenshi WEI
Journal of Clinical Neurology 1992;0(01):-
Objective To verify the variation of mitogen-activated protein kinase kinase kinase 5 (MAPKKK5) gene expression in patients with cryptogenic refractory temporal lobe epilepsy (TLE) and to evaluate the possible molecular pathogenesis of intractable TLE. Methods Reverse transcription polymerase chain reaction (RT-PCR) and Western-blot analysis were used to measure the expression alterations of MAPKKK5 mRNA as well as its protein product MAPKKK5 in temporal cortex samples from patients who had undergone temporal lobectomy for intractable epilepsy (n=10). Tissues from 10 subjects who did not have epilepsy served as controls. Results The expression of MAPKKK5 mRNA (1.001?0.321) and its protein MAPKKK5 (0.359?0.299) were significantly increased in epileptic brain compared with the controls (0.648?0.157, 0.137?0.084, respectively) (all P
2.Genetic characteristics of measles virus strains causing two outbreaks in Guizhou province
Xiaomin TANG ; Li ZHANG ; Xufang YE ; Gang REN ; Li ZUO
Chinese Journal of Microbiology and Immunology 2016;36(12):924-929
Objective To analyze the genetic characteristics of measles virus strains causing two outbreaks in Guizhou province from November 2014 to March 2015. Methods Throat swab samples collect-ed from measles cases in two outbreaks were inoculated into Vero/SLAM cells. Viral RNAs were extracted from positive cultures. Nucleoprotein genes were amplified by using reverse transcription-polymerase chain reaction ( RT-PCR) and the PCR products were sequenced and analyzed. Results Eleven strains of wild-type measles virus were isolated from the two measles outbreaks and all of them belonged to H1a sub-geno-type. Phylogenetic analysis showed that those strains were clustered into two distinct branches. Differences in nucleotide and amino acid genetic distances between the 11 strains of measles virus and the WHO reference strain of H1a sub-genotype (Chin9322) were 1. 1%-1. 6% and 0. 7%-3. 4%, respectively. Compared with the reference strain Chin9322 and Guizhou epidemic strains in recent years, six strains showed amino acid sequence mutations in 47 ( G to S) , 82 ( S to G) and 122 ( R to K) sites and two strains had a mutation in 98 ( P-L) site. Conclusion H1a sub-genotype measles virus was the predominant pathogen causing two measles outbreaks in Guizhou province during 2014 to 2015. Moreover, it was also a predominant sub-geno-type circulating in China and Guizhou province. Different measles virus strains of H1a sub-genotype contin-ued to be prevalent in Guizhou province. This study provided some scientific data for the control and elimina-tion of measles in Guizhou province.
3.Adaptation of PBL Teaching Module by Teacher-student Role Transformation
Gang LI ; Zhinong WANG ; Yafei CUI ; Donglan ZUO ; Yiping XU
Chinese Journal of Medical Education Research 2003;0(02):-
PBL teaching module is the developing trend of medical education reform in China.By studying the advanced foreign experiences and modern methods,teachers and students should change the perceptions and roles to adapt to the PBL teaching style in order to propel the reform of the medical education.
4.Preparation of recombination human ?-interferon and its active analysis
Aijun ZUO ; Dongchun LIANG ; Gang GUO ; Jingyu ZHANG
Chinese Journal of Immunology 2001;0(10):-
Objective:To construct an E.coli expressing system of human interferon-?(IFN-?).Methods:Extracted DNA from human blood and PCR human IFN-?, cloned the human IFN-? gene into plasmid T-easy and pBV-220. Expressed human IFN-? in E.coli DH5?, the expressing product was analysed by SDS-PAGE, Western blot and anti-virus capacity test.Results:DNA sequence analysis showed the recombinant plasmid pBV- IFN-? contained human IFN-?. SDS-PAGE and Western blot proved that there were hIFN-? in E.coli DH5? after temperature inducing and the expressing product has anti-virus activity.Conclusion:A human IFN-? E.coli expressing system was constructed successfully, and the recombination human IFN-? has anti-virus activity.
5.THE PERMEABILITY OF BLOOD-BRAIN BARRIER AND PLACENTA TO SELENIUM IN RAT
Zonghao ZHANG ; Qihua ZUO ; Ruiquan TIAN ; Gang YUAN ;
Acta Nutrimenta Sinica 1956;0(01):-
15 rat dams were divided into 2 groups: selenium treated group drank water containing 2 ppm selenium during pregnancy and breast-feeding period, control group drank tap water. The concentration of selenium in brain tissue for dams were 0.73 ?0.11 (Se-treated) and 0.54 ?0.09 ug/g wet tissue (control), for neonates 0.32?0.02 (Se-treated) and 0.25 ? 0.03 (control), and for 20 days old pups 0.53?0.04 (Se-treated) and 0.44?0.06 (control). The results showed that the selenium administered orally could be transfered through placenta or blood-brain barrier of fetus, young pup and adult rat.
6.Design and clinical application of a three-dimensional biomechanical traction appliance for protrusion of intervertebral disc.
Lei-gang YANG ; Yun-gang YANG ; Xiu-ming YANG ; Zuo-yi LIU ; Huai-xing WEN
Chinese Journal of Medical Instrumentation 2002;26(3):190-191
A three-dimensional biomechanical tracting appliance is introduced in the article, which is used to treat the protrusion of intervertebral disc. The appliance is light, practical, adjustable 3D biomechanic, simple and with multiple functions and convenient operation.
Biomechanical Phenomena
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Equipment Design
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Humans
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Intervertebral Disc Displacement
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therapy
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Lumbar Vertebrae
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pathology
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Traction
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instrumentation
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methods
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Treatment Outcome
7.Effect of RNA interference for OPN on MDA-MB-231 cells and the growth of transplanted tumor in nude mice
Li YANG ; Ling WEI ; Wei ZHAO ; Xianrang SONG ; Xingwu WANG ; Gang ZHENG ; Meizhu ZHENG ; Wenshu ZUO
Chinese Journal of General Surgery 2012;27(4):322-325
ObjectiveTo investigate the role of OPN in human breast cancer cell line ( MDA-MB-231) by using small interfering RNA to specifically knockdown OPN expression. MethodsOPN ShRNA expression vector was stably transfected to MDA-MB-231 cell line.The expression of OPN mRNA and protein were analyzed using reverse transcription polymerase chain reaction (RT-PCR)and Western blot,respectively.The growth of MDA-MB-231 cells were observed by MTT.The effect of OPN siRNA on the transplanted tumor growth and tumor hypoxia were assessed in nude mice. ResultsThe expression level of OPN in MDA-MB-231 cells were significantly lower under hypoxia or normoxia(P < 0.05 ).OPN silence with RNAi significantly inhibited the invasion ability and proliferation of MDA-MB-231 cell lines (P < 0.01 ).Inhibition of OPN with RNAi significantly inhibited the growth ability of MDA-MB-231 cells in vivo(P <0.05).The tumor hypoxia significantly decreased(P < 0.05). ConclusionsOPN silence with RNAi can effectively inhibit cell proliferation and tumor growth of MDA-MB-231 cells,and decrease the bypoxia level of MDA-MB-231 transplanted tumor in nude mice.
8.Transactivation of thyroid hormone receptor βΔ on target genes through thyroid hormone responsive element
Wei XIE ; Ronglan ZHAO ; Ying ZHANG ; Aijun ZUO ; Dongchun LIANG ; Bei SUN ; Gang GUO ; Jingyu ZHANG
Chinese Journal of Endocrinology and Metabolism 2011;27(4):335-336
To study the transactivation of a novel thyroid hormone receptor isoform, TR?. pcDNA3. 1-TR? and pGL3-Promoter/thyroid hormone responsive element were co-transfected into COS-7 cells. The expression of reporter gene was detected. It could be increased up to 45 times by T3. TR? and showed the characteristics of a transcription factor.
9.The thyroid hornone receptor in human osteogenic sarcoma cell line MG-63 cell-T3 binding studies with nuclear extracts
Yuhong YANG ; Aijun ZUO ; Zhifeng ZHU ; Xueqin ZHAO ; Gang GUO ; Jingyu ZHANG
Chinese Journal of Endocrinology and Metabolism 2011;27(2):125-127
To establish a method for radioligand binding assay of thyroid hormone receptors(TR)in human osteoblast-like osteosarcoma cell line MG-63 and to estimate the kinetic parameters of putative receptors. The MG-63cell was cultured in Ham's F12, the soluble TR was prepared from the intact nuclear extracts. The binding properties between TR and T3 were performed by using the traditional Scatchard analysis. The apparent Ka of TR in MG-63 is 7.68× 109 L/mol, and MBC(111. 25+ 10.77)fmol/mg protein. The study indicated that MG-63 cells possessed high affinity and limited-capacity of TR in its nuclear extracts. This may serve as the starting and basic work about TR in bone cell.
10.Effect of thyroid hormones on the expression of thyroid hormone receptor isoforms in human osteogenic sarcoma cell line MG-63
Yuhong YANG ; Aijun ZUO ; Zhifeng ZHU ; Xueqin ZHAO ; Gang GUO ; Jingyu ZHANG
Chinese Journal of Endocrinology and Metabolism 2011;27(3):242-245
Objective To examine the expression of thyroid hormone receptor isoforms (TR α1, α2, β1, and β2) in human osteoblast-like cell line MG-63 at the mRNA level and the effect of thyroid hormone (T3 or T4 ) on the expression. Methods Realtime quantitative PCR was performed. Results The expression of TRα1 mRNA was the highest, that was 10. 70± 0.45, TRβ1 was 5.75 ± 0. 10, TRβ2 was 3.34 ± 0. 08, and TRα2 was very low, only (3.66 ±0. 59) × 10-2. Only the expression of TRαl and TRα2 mRNA was down regulated significantly by the treatment of 10-10 ~ 10-6 mol/L T3, and there was a negative correlation between the expression of TRα1 or TRα2 mRNA and the concentration of T3. Conclusion TRα1 plays a primary role in mediating the effects of thyroid hormones in skeletal development.