Objective:To establish a flow injection chemiluminescence determination method with micropipe-chip for speedy determination of uric acid in clinical samples.Methods:Basing on the rule that uric acid can greatly enhance the chemiluminescence emission intensity of K4Fe(CN)6-K3Fe(CN)6-luminol system,the prepared micropipe-chip was used to analyze the uric acid in serum under the selective conditions.Results:The enhancing chemiluminescence intensity was proportional to uric acid concentration over a range 2.0?10-5～1.0?10-2 g/L.The detection limit(3?) was 4.6?10-7 g/L.The relative standard deviation(RSD) was 3.09% and the recovery test results were 94.0%,99.7%,and 107.8%.Conclusion:The manufacture technique of micropipr-chip is simple but the proposed determination method of uric acid in human serum is sensitive,fast and convenient.

Objective: To study the quenchable effect of Selenium to Luminol-H2O2-KMnO4 Chemiluminescence system and to establish a method for the determination of Selenium in serum.Methods: Chemiluminescence system was used to determine the Chemiluminogenic strength of Luminol-H2O2-KMnO4 and the detection of Selenium in serum was realized.Results:A detection limit with 6.5?10-4?g/ml and a 1.5?10-3～6.5?10-1?g/ml linear range were obtained.The average recovery fraction was 94.21% and the relative standard deviation was 2.97%.Conclusion: A satisfactory result for the determination of Selenium in serum by this method is obtained.

Enophthalmos ; etiology ; surgery ; Humans ; Orbital Fractures ; complications ; surgery ; Polyethylenes ; Prostheses and Implants ; Reconstructive Surgical Procedures ; methods ; Surgery, Computer-Assisted

Purpose:To investigate the clinical characteristics, diagnosis and treatment of accessory breast tumors.Methods:Clinical and pathological data of 16 cases of accessory breast tumors were analyzed retrospectively. Results:There were 5 cases of accessory breast fibroadenoma, 2 cases of intraductal papilloma, 9 cases of accessory breast carcinoma. Local mass resection and radical resection were carried out respectively. All these patients had post operative chemotherapy and radiotherapy. 13 cases (81.3%0 were followed up for 1—10 year. As to results, 3 cases died of metastasis in 2,2.5 and 4 years respectively after operation;one case, reoperated because of local recurrence, still survives (over 3 years):and the others recovered well.Conclusions:It is possible for benign or malignant tumor to occur in the accessory breast,and the manifestation of the accessory breast tumors is similar to that of the primary breast tumor. Operation is the first choice for all treatment benign tumor with local mass resection including the whole accessory breast while accessory breast carcinoma should be treated by radical dissection.

Purpose: To investigate the diagnosis, pathological characteristics and treatment of primary malignant lymphoma of the breast (PMLB). Methods: Clinical and pathological data of 16 cases of PMLB were analyzed retrospectively. Results: No case was correctly diagnosed before operation. All the 12 cases were operated and identified by pathology, in which total mastectomy, radical mastectomy and modified radical mastectomy were carried out in 5, 3 and 4 patients respectively. Moreover, all cases were combined with post-operative chemotherapy or radiotherapy. 10 cases were followed up 1 - 10 years. 4 cases who survived 22 months on the average died of the tumor spread and metastasis during follows-up, and the others are still alive. Especially, 1 case, recurring 2 times locally, has survived for 4. 5 years after reoperation. Conclusions: The final diagnosis of this disease depends on the pathological examination. The majority of PMLB is non-Hodgkins lymphoma ( NHL), and mostly of B cell origin and are mostly related to the mucosa-associated lymphoid tissue ( MALT). Total mastectomy or mastectomy plus axillary nodes dissection is the preferred method of operation and the adequate post-operative chemotherapy is very important.

This paper reports a method for direct determination of ascorbic acid by ultra-violet differential spectrometry and its application to analysis of ascorbic acid contents in vegetables, fruits and tablets. All the four (1st to 4th) derivatives of spectrometry were available for determination, and the better results were obtained from the 2nd derivative spectrometry. The results showed that this method was simple, rapid and accurate.

Arrhythmias, Cardiac ; pathology ; physiopathology ; Humans ; Purkinje Fibers

At the time of phage’s discovery, phage therapy was regarded as a possible treatment method against bacterial infection. Although phage therapy was used to treat and prevent bacterial infection in the former Soviet Union and Eastern Europe, it was abandoned by the West in the 1940s with the arrival of the antibiotic era. However, the ongoing evolution of bacterial multidrug-resistance has recently motivated the Western scientific community to reevaluate phage therapy for bacterial infections that are incurable by conventional chemotherapy. With the indepth study of phages, it’s increasingly acknowledged that phages, as the medicine to cure bacterial infection, are convenient, safe and efficient therapeutics. This paper summarizes the recent years’ advanced researches in this area.

Objective To explore the pathogenesis-related imprinted genes in osteosarcoma and investigate the role of imprinted genes expression in osteosarcoma pathogenesis.Methods The model of malignant transformed immortalized human osteoblastic cells was established and the malignant transformed cells were collected on day 40,55,70,90.The untreated cells passaged from the normal cells on day 90 served as control.After the total RNA extraction,the finally synthesized biotinylated cDNAs were hybridized to CapitalBio Genechip CAP-F0017.2 of the differentially expressed genes.Two genes were chosen at random to further confirm the array results using the SYBR Green real-time PCR in samples of control,day 40 and day 90. Results By an entrance limit of ≥2.0 or ≤0.5,ten imprinted genes which biological functions are mainly related with cell growth/maintenance and signaling transduction were detectable for notablely differential expression,including down-regulated genes of CD81,GRB10,NDN,MEST and up-regulated genes of H19,MKRN3,SLC22A1L,TSSC3,CDKN1C in the malignant transformation of immortalized human osteoblastic cells.The array results were further confirmed by the real-time RT-PCR.Conclusion Ten imprinted genes were detectable for notablely differential expression.Our results will promote our understanding of the molecular mechanisms of imprinted genes in the course of osteosarcoma pathogenesis.

0.05). In hFOB, the expression of P53 was not detected, and that of P63 was weakly positive. The expression of P53 and P63 in HOS transformed by niekel sulfate were higher than that in normal HOS (P