Animals ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Female ; Heat Stress Disorders ; drug therapy ; metabolism ; Hypothalamus ; metabolism ; Male ; Neurotensin ; metabolism ; Phytotherapy ; Rats ; Rats, Wistar ; Tablets

Circulating tumor cells are closely related to the development and progression of malignant tumors. Current methods of detecting circulating tumor cells include reverse transcription polymerase chain reaction method and immunomagnetic enrichment assay. Detection of circulating tumor cells can contribute to early detection of cancer micrometastases, re-classification of clinical stage, monitoring recurrence and metastasis after surgery or radiotherapy and chemotherapy, predicting prognosis, and selecting personalized therapeutic strategy.

Humans ; Immunohistochemistry ; Lymphoma, Large B-Cell, Diffuse ; classification ; pathology

BACKGROUND: Metal on metal (MoM) hip prosthesis has been used for more than 30 years. With the increasing number of young patients who used it, the metal ions cumulative in the body may cause the biological effects, such as cell toxicity, genetic toxicity, carcinogenicity and metal allergy, etc, which have received attention and concern. OBJECTIVE: To describe what influences the production of metal ions, and how metal ions distribute in human tissue after MoM hip prosthesis implantation. In particular the change of the metal ions serum level and the MoM hip prosthesis biological effects of the pathological manifestations were investigated. This paper also focused on the factors influencing metal ions serum level and the mechanism of metal ions biological effects. RETRIEVAL STRATEGY: A computer-based search of Pubmed database was undertaken to identify the articles between January 2004 and November 2007, with the key items of "metals, total hip replacement" and language was limited to English. Meanwhile the China Journal Full-text Database was retrieved for the related articles published from January 2000 to November 2007, with the words of "joint replacement, metal ions" in Chinese. Totally 267 articles were screened out, and checked primarily. Inclusion criteria: ①prosthesis interface described the articles for MoM;②procedure described in the articles for hip replacement;③ articles focused on biological effects of metal ions. Exclusion criteria: ①non-MoM interface of the prosthesis;②non-hip arthroplasty;③articles described the metallurgy;④reproducibility study. LITERATURE EVALUATION: Both titles and abstracts were screened preliminarily, excluding 194 articles of unrelated objectives, 58 articles of repeated contents and 0 Meta analysis. Finally 30 articles were taken into further analysis, including 6 reviews and other basic or clinical trials. DATA SYNTHESIS: MoM prosthesis releases metal ions in the body through electrochemical process (corrosion) and enhanced machinery plus electrochemical process (wear-corrosion). It spreads to various body tissues by body fluid, and raises a number of biological effects. Human body shows a reaction of metal ions in a dose-dependent manner, and metal ions serum level decides the biological effects. Generally we consider the serum concentration of metal ions is influenced by ①ion excretion factors. ② mechanical factors. ③individual factors. ④others. At present, the biological effects of metal ions include ①inflammatory response, ②oxidative damage, ③apoptosis, ④osteoporosis and extracellular matrix dissolution, ⑤carcinogenesis, ⑥host response. Its mechanism is related with corrosion, macrophage activation, reactive oxygen generation, osteoblast function inhibition, osteoclast activation, metal-protein complex formation, and so on. The consequence is a series of pathological changes: ①foreign body granuloma synovitis, ②draining lymph node lesions, ③chromium calmly disease, ④malignant lesions, ⑤metal allergy. CONCLUSION: There is no better product to replace MoM hip prosthesis. Through the study of their biological effects, people gradually concern about two issues. First: What is a safe wear rate? Second: What is harmful serum level of metal ions?

Objective To study the changes of expression levels of vascular endothelial growth factor(VEGF) and its receptors(FLT-1,FLK-1) mRNA in equal area of penumbra in diabetes rats with cerebral infarction(DMCI).Methods Middle cerebral artery occlusion(MACO) model was made in diabetes rats.The expression levels of VEGF and FLT-1,FLK-1 mRNA in equal area of penumbra were measured with situ hybridization in different times after MACO(1 h,3 h,6 h,12 h,24 h).The difference was compared between DMCI group and no diabetes cerebral infarction(NDMCI) group.Results The expression level of VEGF mRNA was lower in DMCI group than that of NDMCI group at every time point(P

Objective: To study the scavenging capacity of extract from four kinds of barley leaves on superoxide free radical and hydroxyl free radical. Methods: Measure the content of superoxide free radical and hydroxyl free radical by nitro blue tetrazolium(NBT) photo-reduction. Results: Along with the flavonoid in the reaction liquid, the scavenging rate shows ascending trend . When the concentration of flavonoid is 12?g/ml, its scavenging rate is 95.56% and 94.12% on superoxide free radical and hydroxyl free radical respectively. Conclusion: The flavonoids of barley leave s have stronger anti-oxidative action.

[Objective]The best treatment method for cemented acetabular component loosening during revision hip replacement has not been established.The purpose of this study is to determine the initial results obtained with a unique implant the modular porous tantalum cup,designed as an alternative treatment for aseptic prosthesis loosening following total hip arthroplasty.[Method]From Apr. 2006 to Nov.2006,porous tantalum cups were implanted during sixteen revision total hip replacements in nine women and seven men who had an average age of 69.2 years at the time of the procedure.Harris score and UCLA activity score were measured before,at 1,2 years and after arthroplasty.Radiographic follow-up was performed simultaneously.All patients were followed-up clinically and radiographically.[Result]The mean follow-up period was 24 month (range18-25months).The mean preoperative Harris hip score(HHS) was 21-54 (32.6) compared with the postoperative HHS of 56-90 (87.5).The mean preoperative UCLA score was 2-5(3.8) compared with the postoperative score of 6-9 (8.2).Radiographic evaluation of the revised components demonstrated no evidence of loosening or subsidence,and all showed radiographic evidence of bone ingrowth.[Conclusion]At the time of short-term follow-up,the porous tantalum cup effectively provided structural stability for acetabular component failure of cemented primary fixation.The potential for long-term biologic fixation may provide durability for porous tantalum cup.Long-term follow-up and comparison with alternative revision techniques will be required to evaluate the true effectiveness of this treatment approach.

Objective Video-assisted thoracoscopic surgery is widely used in clinical practice,due to the characteristics of minimal invasion,quick recovery,low incidence of postoperative chest pain and the same effect of lobectomy as thoracotomy.However,the surgical itself has a certain degree of difficulty,and due to the effect of lymph node,intraoperative blood loss,pleural adhesions,conversion to thoracotomy surgery is needed in some cases. Understanding the risk factors of conversion to thoracotomy can help thoracic surgeons figure out the right time to operate the surgery.

Objective To investigate the role of endoplasmic reticulum stress ( ERS) in asbestos-induced Alveolar epithelial cell apoptosis.Methods A549 cells were treated with asbestos to observe the expression of ERS proteins and apop-tosis gene by immunofluorescence staining and western blotting.Results Under asbestos exposure, the expression of Bip, GRP94, IRE-1α, BAX and BAK are up-regulated.There is a positive correlativity of the expression of proteins to the ex-posure time of asbestos.Conclusion Endoplasmic reticulum stress takes part in asbestos-induced apoptosis.

Objective To study the analgesic effect of sufentanil combined with Dezocine in endoscopic resection of fallopian.MethodsA total of 188 patients with tubal pregnancy from April 2013 to December 2014 in Haining central hospital were enrolled in the study, they were randomly divided into two groups (n=94).On the basis of conventional intravenous analgesia, the patients in control group was were received sufentan, the observation group were given sufentan combined with dezocine.The score of pain at different time points after operation, the Ramsay sedation score, and the level of IL-6 and IL-10 were compared.The adverse reactions were compared between the two groups.ResultsThe score of VAS in the observation group was significantly lower than that in the control at 4h, 8h, 12h and 24h after operation (P<0.05).The score of VAS in the two groups were similar at 48h after operation;the Ramsay score in the observation group was significantly higher than that in the control group(P<0.05) at five time points (4h, 8h, 12h, 24h and 48h after operation);when compared Compared with the indexes before operation, the level of IL-6 was significantly increased in the two groups at 24h and 48h, but the observation group increased less, the IL-6 in observation group was lower than that in the control group at the two points(P<0.05);while the IL-10 was higher than that beforeoperation, and the observation group was more higher(P<0.05).The adverse reactions in the two groups were mainly dizziness, nausea, pruritus and lethargy, and the incidence was only 12.8% in the observation group, which lower than that in the control of 29.8%(P<0.05).ConclusionIt which dazocine combined with sufentanil had a good analgesic effect in the process of analgesia in gynecological laparoscopic resection of fallopian tube after the operation.The VAS score and Ramsay sedation score was improved significantly.The levels of inflammatory factors were lower, and the incidence of adverse reaction was significantly reduced.It is a reliable method for clinical intravenous analgesia.