China ; epidemiology ; Dental Caries ; epidemiology ; Evidence-Based Medicine ; Humans ; Oral Health ; Oral Hygiene ; Periodontal Diseases ; epidemiology ; Practice Guidelines as Topic ; Prevalence

p9O ribosomal S6 kinases(BSKs)are overexpressed in 50% human breast cancer. RSKs enhance the proliferation of breast cancer cells by regulating several key breast cancer-related proteins. They also improve survival of the breast cancer cells through regulating translocation and translation of mRNA. In addition. RSK promotes tumor angiogenesis by interaction with estrogen receptor. However, RSK4 may play an inhibitory role in breast cancer. In general, except RSK4,RSKs may become promising targets of breast cancer therapy.

Objective To observe effect of Rho kinase inhibitor combined with hyperbaric oxygen on the recovery of neurological function after spinal cord injury in patients with thoracolumbar fractures.Methods A total of 80 cases of thoracolumbar fractures combined with spinal cord injury patients as the research object, the packet by the standards of admission of single and double serial number, odd numbers were divided into control group, even number were divided into treatment group (40 cases in each group); two groups underwent surgical treatment, control group was given single usage fasudil treatment, treatment group was given hyperbaric oxygen therapy for auxiliary therapy on the basis of control group, Continuous treatment for two months.Compared neurological function scoring, grading, blood superoxide dismutase (SOD) , epithelial growth factor (EGF) , lipid peroxide (LPO) and adverse reactions pre-and post-treatment between two groups of patients.Results After treatment,total effective rate of treatment group (92.50%), ASIA sensory, movement scores were significantly higher than control group (75.00%)(P<0.05);Content of SOD and EGF of treatment group were significantly higher than control group(P<0.05);the adverse reaction rate (12.50%) was significantly lower than that of control group (32.50%)(P<0.05).Conclusion Rho kinase inhibitor combined with hyperbaric oxygen can significantly improve the degree of injury, neurological function and blood SOD, EGF, LPO content in patients with lumbar vertebral fractures combined with spinal cord injury, and the adverse reactions are few, has definite clinical effect.

Objective: To explore influence of abnormal glucose metabolism on vascular endothelial injury in patients with essential hypertension (EH). Methods: A total of 46 pure EH patients (EH group) and 33 EH patients complicated type 2 diabetes mellitus (T2DM, EH + T2DM group) were enrolled. Blood glucose, blood lipid, body mass index (BMI), serum concentrations of homocysteine (Hcy) and urine microalbumin were measured and compared between two groups. Relationship among serum Hcy, urine microalbumin concentrations and blood glucose, blood lipids, BMI were analyzed. Results: Compared with EH group, there were significant increase in levels of BMI, fasting blood glucose (FBG), 2h postprandial blood glucose (2hPBG), glycosylated hemoglobin (HbA1c), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C) and apolipoprotein B (ApoB), (P<0.05 or <0.01), and even more significant increase in serum levels of Hcy [(12.78±2.51) μmol/L vs. (16.26±2.91) μmol/L] and urine microalbumin [(19.45±5.24) mg/L vs. (33.65±10.70) mg/L] in EH + T2DM group, P<0.01 both; Pearson correlation analysis indicated that in EH patients with DM, serum Hcy level was positively correlated with BMI, FBG, HbA1c, LDL-C, ApoB and urine microalbumin (r=0.667~0.906，P<0.01 all), while urine microalbumin level was positively correlated with BMI, HbA1c, LDL-C, ApoB and serum Hcy (r=0.566~0.685, P<0.01 all). Conclusion: Abnormal glucose metabolism can aggravate both vascular endothelial injury and renal microvascular injury in patients with essential hypertension, and these are closely related with degree of abnormal glucose metabolism. Therefore, controlling blood glucose level can relieve vascular injury, further relieve pathological development of cardiovascular diseases as well as renal complications.

Objective: To explore the influence of impaired glucose metabolism (IGM) on cardiovascular function in patients with essential hypertension (EH). Methods: A total of 46 pure EH patients and 36 EH + type 2 diabetes mellitus (T2DM) patients were selected. Levels of blood glucose, blood lipids, serum uric acid (UA), fibrinogen (Fb), serum homocysteine (Hcy) and urinary microalbumin were measured, and patients received 24h ambulatory blood pressure monitoring (ABPM) and color Doppler echocardiography. Clinic indexes, ambulatory blood pressure parameters and incidence rate of cardiac diastolic dysfunction were compared and analyzed between two groups. Cardiac diastolic dysfunction was regarded as a binary dependent variable, and it underwent multi-factor gradual binary regression analysis. Results: Body mass index (BMI), blood glucose, blood lipids (except high density lipoprotein cholesterol, apoprotein AI), UA, Fb, serum Hcy and urinary microalbumin levels in EH + T2DM group were significantly higher than those of pure EH group (P<0.05 or P<0.01), and their 24h mean systolic blood pressure (24hSBP), daytime mean SBP (dSBP), 24h mean pulse pressure (24hPP) and daytime mean PP (dPP) were significantly higher than those of pure EH patients (P<0.05 or P<0.01). Left ventricular ejection fraction (LVEF) of both groups was > 40%, and incidence rate of cardiac diastolic dysfunction in EH + T2DM group (72.2%) was significantly higher than that of pure EH group (45.7%), P<0.05. Binary Logistic regression analysis indicated that age (OR=1.160, 95%CI: 1.002~1.342, P=0.012), DM (OR=3.095, 95%CI: 1.056~9.079, P=0.029) and glycosylated hemoglobin (HbA1c, OR=1.756, 95%CI: 1.261~2.445, P=0.008) were independent risk factors for cardiac diastolic dysfunction in EH patients. Conclusion: Impaired glucose metabolism aggravates cardiovascular system dysfunction in patients with essential hypertension through aggravating atherosclerosis and cardiac early diastolic dysfunction. Therefore, improvement of glucose metabolism in these patients could help to reduce their risk of cardiovascular diseases.

This study was aimed to improve the therapeutic results of composite skin substitutes. Human vascular endothelial cell growth factor (hVEGF 165 ) gene was constructed to an eukaryotic expression vector pcDNA3. Transfection of recombinant vector pcDNA3 hVEGF 165 into human dermal fibroblast cells was performed. VEGF protein level in the supernatant of transfected fibroblasts culture was determined. Its biological activities were tested by observing the growth rate of the human umbilical vein endothelial cells/HUVEC after being stimulated with the said supernatant, and by performing the Miles assay in guinea pigs. The results showed that these transgenic cells were able to secrete VEGF to certain extent, with biological activities to enhance the growth of HUVEC in vitro and improve vascular permeability. It indicated that transgenic fibroblasts could resurface the dermal substitute of a composite skin.

In order to cultivate students' quality of scientific research thinking,self-learning ability,innovation ability and practical ability,Open Innovation Experiment Project which is suitable for the undergraduate students has been carried out in Jilin university.The feature of experiment is based on the teacher's scientific research project,and the experimental design is completed by the students independently.By the stage inspection as a monitoring mechanism,the overall effect evaluation of the experimental project has been conducted by the combination of the process and the results.Through the selection,design,implementation and results of practice,students can not only cultivate innovative consciousness and comprehensive thinking and the spirit of unity and cooperation,but also have a basic understanding of scientific research work.

Objective To study the MRI features of sporadic Creutzfeldt-Jakob disease(sCJD). Methods Three patients with clinically diagnosed sCJD underwent MR study,including SE T_1WI,FSE T_2 WI,and DWI sequences.The MR imaging features were analyzed.Results The lesions were not definite either in SE T_1 WI or in FSE T_2 WI,but were prominent in DWI.Abnormal hyperintensive signal appeared in the cerebral cortex,with the frontal,parietal,and occipital lobes being the mostly involved region.The subcortical white matter was normal.The bilateral caudate nuclei and thalami could also be involved.The abnormal signal could be either symmetrical or asymmetrical.There was diffuse atrophy of the brain parenchyma in the late phase of disease,especially in the cortex.Conclusion With the application of MR study,especially the DWI,combined with its characteristic clinical manifestation,the diagnosis of sCJD can be made definitely.

Objective To establish a cellulose acetate membrane electrophoretic method for separating the exogenous sulfated poly mannuguluronicate(911) from glycosaminoglycans.Methods 911 both in urine and ultrafiltrated urine were extracted with cetylpyridinium chloride (CPC), and then separated by two step electrophoretic method with two different buffer solutions. Results The results indicated that 911 was thoroughly separated from endogenous CSC and DS with its average recovery in urine approximately at 70%. Furthermore, the lowest sensitivity for determination of 911 in aquatic solution was 100 ?g?L -1 , whereas that in urine 250 ?g?L -1 and in ultrafiltrated urine 500 ?g?L -1 . And most importantly 911 in rat's urine was detectable after it's oral administration at dose of 200mg?kg -1 . Conclusions It is the first time for us to conduct the existence of 911 in urine by two step electrophoretic method with two different buffer solutions, suggestive of the feasibility to determine 911 in urine even following it's oral administration.