p9O ribosomal S6 kinases(BSKs)are overexpressed in 50% human breast cancer. RSKs enhance the proliferation of breast cancer cells by regulating several key breast cancer-related proteins. They also improve survival of the breast cancer cells through regulating translocation and translation of mRNA. In addition. RSK promotes tumor angiogenesis by interaction with estrogen receptor. However, RSK4 may play an inhibitory role in breast cancer. In general, except RSK4,RSKs may become promising targets of breast cancer therapy.

Objective: To explore influence of abnormal glucose metabolism on vascular endothelial injury in patients with essential hypertension (EH). Methods: A total of 46 pure EH patients (EH group) and 33 EH patients complicated type 2 diabetes mellitus (T2DM, EH + T2DM group) were enrolled. Blood glucose, blood lipid, body mass index (BMI), serum concentrations of homocysteine (Hcy) and urine microalbumin were measured and compared between two groups. Relationship among serum Hcy, urine microalbumin concentrations and blood glucose, blood lipids, BMI were analyzed. Results: Compared with EH group, there were significant increase in levels of BMI, fasting blood glucose (FBG), 2h postprandial blood glucose (2hPBG), glycosylated hemoglobin (HbA1c), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C) and apolipoprotein B (ApoB), (P<0.05 or <0.01), and even more significant increase in serum levels of Hcy [(12.78±2.51) μmol/L vs. (16.26±2.91) μmol/L] and urine microalbumin [(19.45±5.24) mg/L vs. (33.65±10.70) mg/L] in EH + T2DM group, P<0.01 both; Pearson correlation analysis indicated that in EH patients with DM, serum Hcy level was positively correlated with BMI, FBG, HbA1c, LDL-C, ApoB and urine microalbumin (r=0.667~0.906，P<0.01 all), while urine microalbumin level was positively correlated with BMI, HbA1c, LDL-C, ApoB and serum Hcy (r=0.566~0.685, P<0.01 all). Conclusion: Abnormal glucose metabolism can aggravate both vascular endothelial injury and renal microvascular injury in patients with essential hypertension, and these are closely related with degree of abnormal glucose metabolism. Therefore, controlling blood glucose level can relieve vascular injury, further relieve pathological development of cardiovascular diseases as well as renal complications.

Objective: To explore the influence of impaired glucose metabolism (IGM) on cardiovascular function in patients with essential hypertension (EH). Methods: A total of 46 pure EH patients and 36 EH + type 2 diabetes mellitus (T2DM) patients were selected. Levels of blood glucose, blood lipids, serum uric acid (UA), fibrinogen (Fb), serum homocysteine (Hcy) and urinary microalbumin were measured, and patients received 24h ambulatory blood pressure monitoring (ABPM) and color Doppler echocardiography. Clinic indexes, ambulatory blood pressure parameters and incidence rate of cardiac diastolic dysfunction were compared and analyzed between two groups. Cardiac diastolic dysfunction was regarded as a binary dependent variable, and it underwent multi-factor gradual binary regression analysis. Results: Body mass index (BMI), blood glucose, blood lipids (except high density lipoprotein cholesterol, apoprotein AI), UA, Fb, serum Hcy and urinary microalbumin levels in EH + T2DM group were significantly higher than those of pure EH group (P<0.05 or P<0.01), and their 24h mean systolic blood pressure (24hSBP), daytime mean SBP (dSBP), 24h mean pulse pressure (24hPP) and daytime mean PP (dPP) were significantly higher than those of pure EH patients (P<0.05 or P<0.01). Left ventricular ejection fraction (LVEF) of both groups was > 40%, and incidence rate of cardiac diastolic dysfunction in EH + T2DM group (72.2%) was significantly higher than that of pure EH group (45.7%), P<0.05. Binary Logistic regression analysis indicated that age (OR=1.160, 95%CI: 1.002~1.342, P=0.012), DM (OR=3.095, 95%CI: 1.056~9.079, P=0.029) and glycosylated hemoglobin (HbA1c, OR=1.756, 95%CI: 1.261~2.445, P=0.008) were independent risk factors for cardiac diastolic dysfunction in EH patients. Conclusion: Impaired glucose metabolism aggravates cardiovascular system dysfunction in patients with essential hypertension through aggravating atherosclerosis and cardiac early diastolic dysfunction. Therefore, improvement of glucose metabolism in these patients could help to reduce their risk of cardiovascular diseases.

Objective To observe effect of Rho kinase inhibitor combined with hyperbaric oxygen on the recovery of neurological function after spinal cord injury in patients with thoracolumbar fractures.Methods A total of 80 cases of thoracolumbar fractures combined with spinal cord injury patients as the research object, the packet by the standards of admission of single and double serial number, odd numbers were divided into control group, even number were divided into treatment group (40 cases in each group); two groups underwent surgical treatment, control group was given single usage fasudil treatment, treatment group was given hyperbaric oxygen therapy for auxiliary therapy on the basis of control group, Continuous treatment for two months.Compared neurological function scoring, grading, blood superoxide dismutase (SOD) , epithelial growth factor (EGF) , lipid peroxide (LPO) and adverse reactions pre-and post-treatment between two groups of patients.Results After treatment,total effective rate of treatment group (92.50%), ASIA sensory, movement scores were significantly higher than control group (75.00%)(P<0.05);Content of SOD and EGF of treatment group were significantly higher than control group(P<0.05);the adverse reaction rate (12.50%) was significantly lower than that of control group (32.50%)(P<0.05).Conclusion Rho kinase inhibitor combined with hyperbaric oxygen can significantly improve the degree of injury, neurological function and blood SOD, EGF, LPO content in patients with lumbar vertebral fractures combined with spinal cord injury, and the adverse reactions are few, has definite clinical effect.

China ; epidemiology ; Dental Caries ; epidemiology ; Evidence-Based Medicine ; Humans ; Oral Health ; Oral Hygiene ; Periodontal Diseases ; epidemiology ; Practice Guidelines as Topic ; Prevalence

This study was aimed to improve the therapeutic results of composite skin substitutes. Human vascular endothelial cell growth factor (hVEGF 165 ) gene was constructed to an eukaryotic expression vector pcDNA3. Transfection of recombinant vector pcDNA3 hVEGF 165 into human dermal fibroblast cells was performed. VEGF protein level in the supernatant of transfected fibroblasts culture was determined. Its biological activities were tested by observing the growth rate of the human umbilical vein endothelial cells/HUVEC after being stimulated with the said supernatant, and by performing the Miles assay in guinea pigs. The results showed that these transgenic cells were able to secrete VEGF to certain extent, with biological activities to enhance the growth of HUVEC in vitro and improve vascular permeability. It indicated that transgenic fibroblasts could resurface the dermal substitute of a composite skin.

In order to cultivate students' quality of scientific research thinking,self-learning ability,innovation ability and practical ability,Open Innovation Experiment Project which is suitable for the undergraduate students has been carried out in Jilin university.The feature of experiment is based on the teacher's scientific research project,and the experimental design is completed by the students independently.By the stage inspection as a monitoring mechanism,the overall effect evaluation of the experimental project has been conducted by the combination of the process and the results.Through the selection,design,implementation and results of practice,students can not only cultivate innovative consciousness and comprehensive thinking and the spirit of unity and cooperation,but also have a basic understanding of scientific research work.

A rat model of metabolic syndrome was induced by high glucose plus high fat diet and treated by tea polyphenols for 10 weeks.The results showed that the levels of fasting blood glucose,triglyceride,cholesterol,low-density lipoprotein cholesterol,and free fatty acid in tea polyphenols treatment group were significantly lower than those in control group(all P＜0.05),along with decreased TNF-α,IFN-γ,iNOS mRNA and protein expressions,and IL-1β protein expression in pancreatictissue (all P＜0.05).Under electron microscope,more secretory granules in islet beta cells and impoveddisorganization of cellular organ were shown in tea polyphenols treatment group compared with metabolic syndrome group.The results suggest that tea polyphenols are able to protect islet β cells against oxidative damage via inhibiting the production of inflammatory cytokines.

Objective:To study the expression and clinical significance of hypoxia-inducible factor-1? (HIF-1?) in squamous cell carcinoma of buccal mucosa.Methods:45 cases of paraffin embedded samples of squamous cell carcinoma of buccal mucosa were examined with HIF-1? antibody by immunohistochemistrical method. The relationship between HIF-1? expression and clinico-pathological factors such as sex, age, duration of tumour, T classification, differentiation, lymph node metastasis in the neck, recurrence, clinic classification, tumour thickness, the number of mitosis and the degree of lymphocyte invasion was analyzed.Results:The positive expression of HIF-1? was found in 31 out of the 45 cases(69%). HIF-1? positive reaction was predominately located in the center of tumour nest. While analyzing clinico-pathological factors, only T classification (between T1+T2 and T3+T4, P=0.008), lymph node metastasis of the neck (P=0.037) and clinic classification (between stage I+II and III+IV, P=0.011) showed a significant correlation with HIF-1? expression. HIF-1? possitive expression is higher in T3+T4(d=4.87 mm) than that in T1+T2(3.81 mm) (P=0.046). Logistic analysis showed that the longer the patients lived after operation, the less the HIF-1? positive expression.Conclusions:HIF-1? positive expression in squamous cell carcinoma of the buccal mucosa correlates to T classification, lymph node metastasis of neck, clinical classification and survival