Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Bone Neoplasms ; pathology ; therapy ; Catheter Ablation ; Humans ; Lung Neoplasms ; secondary ; surgery ; Neoplasm Recurrence, Local ; Osteosarcoma ; pathology ; secondary ; therapy ; Salvage Therapy

Objective: To determine the contents of calycosin-7-O-β-D-glucoside and formononetin in Astragalus membranaceus (Fisch.) Bge. by high performance liquid chromatography (HPLC). Methods: The HPLC condition was as follows: column: Hypersil ODS 2 column (4.6 mm×250 mm, 5 μm); mobile phase: A was ACN-MeOH (9 : 1,V /V), B was H2O, with gradient elution; flow speed: 1.0 ml/min; detection: 260 nm; temperature of column: room temperature; injection volume: 20 μl. Astragalus membranaceus (Fisch.) Bge. was extracted with methanol solution twice, each time 20 min. Results: The theoretical plate numbers of calycosin-7-O-β-D-glucoside and formononetin were 50 134 and 25 258, respectively. The calibration curves were linear within the range of (2.022-101.1) μg/ml for calycosin-7-O-β-D-glucoside and (38.04-1522) μg/ml for formononetin, with their regression function being Y=58 924X - 12 352, r=0.999 9 and Y=9 237X - 124 447, r=0.999 9, respectively. The intra-day and inter-day precisions (RSD) at low, middle and high injection volume were all less than 2.0%. The limits of detection were 0.202 2 mg/ml for calycosin-7-O-β-D-glucoside and 1.522 mg/ml for formononetin. The recovery rates were 98.34% (RSD=1.33%, n=3) for calycosin-7-O-β-D-glucoside and 98.84% (RSD=0.12%, n=3) for formononetin. The contents of calycosin-7-O-β-D-glucoside and formononetin in 10 different batch of Astragalus membranaceus (Fisch.) Bge. were determined. Conclusion: HPLC is a simple and reliable method for determining the contents of calycosin-7-O-β-D-glucoside and formononetin in Astragalus membranaceus (Fisch.) Bge.

Objective: To establish an HPLC method for determination of baicalin and wogonoside contents in Xiaochaihu decoction. Methods: Decoction pieces were mixed and decocted with water. The chromatographic column was Agilent Zorbax XDB-C18 (150 mmX4.6 mm, 5 μm); the mobile phase was composed of 0.2% phosphate acid-water(A) and acetonitrile (B) with gradient elution at a flow rate of 1 ml/min. The detection wavelength was set at 275 nm, the temperature of column was 25°C, and the injection volume was 15 μL. Results: Baicalin and wogonoside were separated at baseline within 30 min with good linearity; the standard curves for baicalin and wogonoside were Y=44.16X-36.22 (r=0.999,9) and Y=52.08X-28.69(r= 0.999,9), respectively. The intra-day and inter-day precisions were both less than 1%, and the limits of qualification were 0.615,6 μg/ml for baicalin and 0.220,8 μg/ml for wogonoside. The recovery rates (n = 6) were 95.73% (RSD = 0.8%) for baicalin and 97.02% (RSD= 1.56%) for wogonoside. Conclusion: The method is simple, accurate, stable, and reliable in determining the contents of baicalin and wogonoside in Xiaochaihu decoction, and it can be used for the quality control of this preparation.

Objective:To deteat N‐terminal pro B type natriuretic peptide (NT‐pro BNP) and cystatin C (Cys C) con‐tent in Kazak patients with hypertensive heart disease (HHD) complicated heart failure (HF) and analyze the corre‐lation between them .Methods :A total of 100 Kazak HHD patients were divided into hypertension complicated HF group (Complicated HF group , n=50) and pure HHD group (n=50) .Venous blood sample was taken within 24h after hospitalization for measuring serum levels of NT‐proBNP and Cys C ,then they were compared and analyzed between two groups .Results:Compared with pure HHD group ,there were significant rise in serum levels of NT‐proBNP [ (246.53 ± 165.65) ng/L vs .(4568.32 ± 2722.36) ng/L] and Cys C [ (0.82 ± 0.31) mg/L vs .(1.93 ± 2.46) mg/L] in complicated HF group , P< 0.01 both .Spearman correlation analysis indicated that serum NT‐proBNP level was positively correlated with Cys C level in complicated HF group , r=0.961 , P<0.01. Conclusion:In Kazak patients with hypertensive heart disease complicated HF ,serum NT‐proBNP and Cys C levels significantly rise and they significantly positively correlate ,so it suggest there may be slight damaged renal function also .

A possible ?-Galactosidase gene(pwtsA) was discovered from soil metagenomic DNA of Taishan Mountain.PwtsA gene was inserted into the expression vector pET30a and transferred into E.coli BL21(DE3).Recombinant protein PWTSA was expressed as a soluble form at high level through IPTG induction,with a molecular mass of 57 kD analyzed by SDS-PAGE.PWTSA can produce o-nitrophenol from o-nitrophenol-?-D-galactopyranoside(ONPG),and its specific activity was determined as 13.6 U/mg.The enzymatic studies demonstrated that the recombinant protein PWTSA was a thermostable ?-Galactosidase,its optimum temperature and pH were 85?C～95?C and 6.5 respectively.In standard assays,the Km for ONPG was 0.83 mmol/L.

Adenoma ; pathology ; Carcinoma, Renal Cell ; pathology ; Diagnosis, Differential ; Female ; Humans ; Kidney Neoplasms ; pathology ; Middle Aged

Ligusticum Chuanxiong,a traditional Chinese medicine,has the functions of promoting Qi flow and blood,dispelling pathogenic wind and relieving pain.One of effective constituents extracting from Chuanxiong is tetramethylpyrazine,which has several pharmacological activities and anti-tumor function through inhibiting expression of vascular endothelial growth factor(VEGF).Recent relative studies provided scientific basis for a better application oftetramethylpyrazine clinically.

BACKGROUND: Hippocampal formation of brain, a cerebral area related with learning and memory, is closely related to spatial cognitive activity.Peroxidative stress following the onset of cerebral ischemia can induce DNA injury in hippocampal 'area and dentate gyrus, the fall of the ability of DNA plerosis and correspondingly a decline in the function of learning and memory.OBJECTIVE: To investigate the effect of hongjingtian on the expression of nucleic acid of hippocampal area and dentate gyrus, the learning and memory area of rats with complete cerebral ischemia-reperfusion injury.DESIGN: Randomized controlled study.SETTING: Central Laboratory of Armed Police Medical College.PARTICIPANTS: The experiment was completed at the Central Laboratory of Armed Police Medical College from April 2002 to April 2004. Totally 60 Wistar male rats were selected and divided randomly into 5 groups Model group: Rats were perfused daily with distilled water of a volume the perfused daily with distilled water of a volume the same as that in medication group.METHODS: Rats in each group were perfused incessantly for 7 days.Modified Pulsinelli-4 vessel blocking method was used to reproduce the rat model of acute complete cerebral ischemia-reperfusion. Rats in sham-operation group were not treated with the operation of burning vertebral artery and clipping common carotid artery. The changes of DNA and RNA expressions in cerebral hippocampal area and dentate gyrus were observed with acridine orange staining method after model establishment.MAIN OUTCOME MEASURES: Expression of DNA and RNA in cerebral hippocampal area and dentate gyrus of rats in each group.RESULTS: All 60 rats entered the final analysis. DNA and RNA in shamoperation group distributed evenly, border of fluorescent reflex was legible and response intensity was strong. Border of DNA and RNA fluorescent reflex was illegible and response intensity was obviously weak in model ly, border of fluorescent reflex was legible and response intensity was group was not of obvious difference as compared with that in model group.CONCLUSION: The illegibility of DNA and RNA fluorescent response in operation group is related with the breakage of DNA chain induced by oxidative stress during cerebral ischemia-reperfusion injury. Border of DNA and RNA fluorescent reflex in hippocampal area and dentate gyrus is legihibit the breakage of DNA chain induced by oxidative stress and protect learning and memory function in hippocampal area and dentate gyrus of rats with complete cerebral ischemia-reperfusion.

Ear, Middle ; pathology ; Female ; Granuloma, Plasma Cell ; pathology ; Humans ; Mastoiditis ; pathology ; Middle Aged

Objective To investigate the inhibition mechanism of sodium selenite on HCT116 cells.Methods In the present study,we explored the cytotoxicity induced by sodium selenite and the underlying mechanism by MTS assay,WesternBlot,and small RNA interference technique.Results It was found that the sodium selenite at 5uM concentration could indeed reduce the viability of colon cancer cell line HCT116 by a large margin through increasing the generation of reactive oxygen species (ROS),and that the increased levels of ROS could activate c-Jun Nh2-terninal kinase 1 (JNK1).Additionally,knockdown expression of JNK1 or p53 by using RNAi attenuated the cytotoxicity induced by sodium selenite,indicating that both of JNK1 and p53 are required in the process of cell death induced by sodium selenite.Conclusion The sodium selenite could induces cell death in HCT116 through oxidative stress by involvement of JNK1 and p53,both of which play a critical role in toxicity of sodium selenite.