In the initial experiment for the evaluation of the correlativity between McAb-and PcAb-ELISA systemes, we found that the serum IgE levels detected by the McAb-ELISA were about 10 times lower than those detected by the PcAb-ELISA system. Subsequent works demonstrated that this is due to the cross-reaction of PcAb with some non-IgE component(s) in human serum whereas the the possibility that the epitopes directed by our McAbs are not universally present on all IgE molecules, although ean not be definitely excluded at the present time, may not be responsible to the differences.

Objective To analyse the associativity among serum thyroid hormone level,brain injury and neuroethology in preterm infants by testing the thyroid hormone level and neuro-behaviour assessment.Methods Fifty-two preterm infants were continuously admitted in neonatal department of Shanghai Children's Hospital from Dec 2009 to Apr 2010. Radio-immunity was used to determine the serum level of T3,T4, TSH within 6 h after birth. Each case received cranial ultrasonic examination within 3 d after birth and rechecked every week. Before discharge, every infant received a cranial MRI examination. The 52 cases were devided into three groups according to the result of ultrasound and MRI:no brain damage group (33 cases),intraventricular hemorrhage greup (10 cases) ,and white matter injury group (9 cases). At the corrected gestation age 40±2 weeks,every infant received a neonatal behavioral neurological assessment (NBNA). Results The level of serum TSH in all the three groups of preterm infants were normal, which could reject congenital hypothyroidism. Eight preterm infants(15.4% ,8/52) had normal thyroid hormone level,another 44 preterm infants(84. 6% ,44/52) got lower thyroid functions. The levels of T3 and T4 were higher in the no brain damage group than those in intraventricular hemorrhage group and white matter injury group. And the preterm infants who had white mauer injury got the lowest level of thyroine hormone T3 and T4. Thyroxine hormone levels had significant difference among three groups (P ＜ 0. 05). The preterm infants who had no brain damage got higher scores in capability, passive muscle tonus,initiative muscle tonus and total score than the other two groups. Intraventricular hemorrhage group always got higher scores in NBNA than the white matter injury group (P ＜ 0. 05). The NBNA scores had significant difference among three groups (P ＜ 0. 05). Conclusion Premature infant who has more severe brain injury always has lower levels of thyroxine hormone. Premature infants with brain injury get lower scores in NBNA test than those without brain injury.

Humans ; Multiple Myeloma

Acute kidney injury ( AKI) in newborns is one of neonatal critical disease,the incidence of AKI is 23%and the mortality is 25%~50%in neonatal intensive care unit (NICU).AKI in newborns is ab-normal renal function due to many various unfavorable factors,such as neonatal asphyxia,low blood volume, shock,hypoxia,hemolysis,low temperature,the clinical manifestations are oliguria or anuria,electrolyte disturb-ance,acid-base imbalance, high concentrations of metabolites in plasma and renal excretion ( urea, creatinine etc.) .At present,there are no effective prevention measures.In recent years,with the progress of blood purifica-tion technology,continuous blood purification ( CBP) has been used from AKI in children to AKI in newborns. Because of the neonatal hemodynamic instability and blood characteristics of small capacity, the application of CBP in the neonatal AKI still faces many problems.The paper summarizes high risk factors and diagnostic crite-ria of AKI in newborns,related problems of neonatal CBP treatment.

The lumbar facet joints osteoarthritis can be a potentially important source of low back pain.The risk factors of lumbar facet joints osteoarthritis has been systematic reviewed.Recent research shows that risk factors for lumbar facet joints osteoarthritis include advanced age(older than 50 years old),male or post-menopause female,relatively more sagittal orientation of the facet joints,a background of intervertebral disk degeneration and L4、5 spinal levels.An up-to-date knowledge of this subject can be helpful in diagnosis and treatment of low back pain.

We report a case of a 47-year-old man who firstly complained of throat pain for half a month accompanied with fever. Specialized examination showed tonsils' hypertrophy and the laryngoscope found his right vocal cord was swelling and hyperemia. The routine blood test counted white blood cell as 31 x 10(9)/L, lymphocyte as 30. 84 x 10(9)/L while prolymphocyte could be seen with microscope. After that B-ultrasound scan gave spleen hypertrophy and multi-lymphatic-node enlargement. Peripheral blood flowcytometry and bone marrow biopsy finally diagnosed the man as actue myelocytic leukemia.
Humans ; Leukemia, Myeloid, Acute ; complications ; diagnosis ; Male ; Middle Aged ; Pain ; etiology ; Pharynx

Lymphoid neoplasms that are derived from natural killer(NK) cells are uncommon but distinct clinicopathologic disease entities.In recent years,the conceptual view of NK cell neoplasms has changed as the result of further understanding of the cell derivations and the characteristics of the malignant counterparts.In this article,we reviewed the recent concepts and progress in clinicopathologic features,pathogenesis,diagnosis,treatment strategies,and outcomes of this group of uncommon neoplasms.

The effect of molecular cytogenetics in pathogenesis of multiple myeloma (MM) is fundamental.The risk stratification system that based on molecular cytogenetics of MM is widely applicable in the prognosis of MM.The aberration of molecular cytogenetics is the most important marker of MM.At the same time,high-risk MM is associated with intra-clonal tumor heterogeneity and clonal evolution.The research of intra-clonal tumor heterogeneity and clonal evolution which based on the aberration of molecular cytogenetics will provide some additional information of the biology of MM and contribute to the optimum treatment for MM patients.

Cell Proliferation ; Cell Transformation, Neoplastic ; Epigenesis, Genetic ; Hodgkin Disease ; genetics ; immunology ; metabolism ; pathology ; Humans ; Immunophenotyping ; NF-kappa B ; metabolism ; Receptor, Notch1 ; metabolism ; Reed-Sternberg Cells ; immunology ; metabolism ; pathology ; Transcription Factor AP-1 ; metabolism

Objective To investigate the clinical results of microsurgical free tissue transfers in pediatric reconstruction.Methods Between Janua~ 2004 and December 2010,twenty-seven free tissue transfers for reconstruction of various defects in 27 pediatric patients were performed.The average age at the time of reconstruction was 10.8 years(range:5-14 years).Fourteen patients were boys and 13 were girls.There were 5 tumor resection defects,sixteen posttraumatic defects and 6 burn contractures which consist of 19 fasciocutaneous flaps,six osteoseptocutaneous flaps and 2 muscle flaps.The mean operative time was 4 hours and 20 minutes.Results All flaps survived except 1 partial necrosis,with 96.3％ success rate.All patients were followed up 3 months to 4 years (mean,one and a half years).The appearance of the flaps was slightly overstaffed,but the color and texture were satisfactory.Conclusion Free tissue flap transfer is a safe,reliable,cost-and time-effective method for the reconstruction of various defects in children.